---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
PlanS_conform: '1'
_id: '21231'
abstract:
- lang: eng
text: To assess cell migration in complex spatial environments, microfabricated
chips, such as mazes and pillar forests, are routinely used to impose spatial
and mechanical constraints, and cell trajectories are followed within these structures
by advanced imaging techniques. In systems mechanobiology, computational models
serve as essential tools to uncover how physical geometry influences intracellular
dynamics; however, decoding such complex behaviors requires advanced inference
techniques. Here, we integrated experimental observations of dendritic cell migration
in a geometrically constrained microenvironment into a Cellular Potts model. We
demonstrated that these spatial constraints modulate the motility dynamics, including
speed and directional changes. We show that classical summary statistics, such
as mean squared displacement and turning angle distributions, can resolve key
mechanistic features but fail to extract richer spatiotemporal patterns, limiting
accurate parameter inference. To solve this, we applied neural posterior estimation
with in-the-loop learning of summary features. This learned summary representation
of the data enables robust and flexible parameter inference, providing a data-driven
framework for model calibration and advancing quantitative analysis of cell migration
in structured microenvironments.
acknowledgement: 'This work was supported by the German Federal Ministry of Education
and Research (BMBF) (EMUNE/031L0293C), the European Union via the ERC grant INTEGRATE,
grant agreement number 101126146, and under Germany’s Excellence Strategy by the
Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) (EXC 2047—390685813,
EXC 2151—390873048, FOR5775 — 533863915, and 524747443), the University of Bonn
via the Schlegel Professorship of J.H., and the returning experts fellowship of
the Ministry of Innovation, Science, and Research of North-Rhine-Westphalia (AZ:
421-8.03.03.02-137069). J.M. is a member of the Nanofabrication Facility and is
supported by the Institute of Science and Technology Austria. E.K. acknowledges
the TRA Life and Health (University of Bonn) as part of the Excellence Strategy
of the federal and state governments. The authors thank Laeschkir Würthner for his
insightful comments on the implementation of the authors’ model. The views and opinions
expressed are those of the authors only and do not necessarily reflect those of
the funding agencies. Parts of Fig. 1 were created using BioRender. Open Access
funding enabled and organized by Projekt DEAL.'
article_number: '20'
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Jonas
full_name: Arruda, Jonas
last_name: Arruda
- first_name: Emad
full_name: Alamoudi, Emad
last_name: Alamoudi
- first_name: Robert
full_name: Mueller, Robert
last_name: Mueller
- first_name: Marc
full_name: Vaisband, Marc
last_name: Vaisband
- first_name: Ronja
full_name: Molkenbur, Ronja
last_name: Molkenbur
- first_name: Jack
full_name: Merrin, Jack
id: 4515C308-F248-11E8-B48F-1D18A9856A87
last_name: Merrin
orcid: 0000-0001-5145-4609
- first_name: Eva
full_name: Kiermaier, Eva
last_name: Kiermaier
- first_name: Jan
full_name: Hasenauer, Jan
last_name: Hasenauer
citation:
ama: Arruda J, Alamoudi E, Mueller R, et al. Simulation-based inference of cell
migration dynamics in complex spatial environments. npj Systems Biology and
Applications. 2026;12. doi:10.1038/s41540-026-00648-9
apa: Arruda, J., Alamoudi, E., Mueller, R., Vaisband, M., Molkenbur, R., Merrin,
J., … Hasenauer, J. (2026). Simulation-based inference of cell migration dynamics
in complex spatial environments. Npj Systems Biology and Applications.
Springer Nature. https://doi.org/10.1038/s41540-026-00648-9
chicago: Arruda, Jonas, Emad Alamoudi, Robert Mueller, Marc Vaisband, Ronja Molkenbur,
Jack Merrin, Eva Kiermaier, and Jan Hasenauer. “Simulation-Based Inference of
Cell Migration Dynamics in Complex Spatial Environments.” Npj Systems Biology
and Applications. Springer Nature, 2026. https://doi.org/10.1038/s41540-026-00648-9.
ieee: J. Arruda et al., “Simulation-based inference of cell migration dynamics
in complex spatial environments,” npj Systems Biology and Applications,
vol. 12. Springer Nature, 2026.
ista: Arruda J, Alamoudi E, Mueller R, Vaisband M, Molkenbur R, Merrin J, Kiermaier
E, Hasenauer J. 2026. Simulation-based inference of cell migration dynamics in
complex spatial environments. npj Systems Biology and Applications. 12, 20.
mla: Arruda, Jonas, et al. “Simulation-Based Inference of Cell Migration Dynamics
in Complex Spatial Environments.” Npj Systems Biology and Applications,
vol. 12, 20, Springer Nature, 2026, doi:10.1038/s41540-026-00648-9.
short: J. Arruda, E. Alamoudi, R. Mueller, M. Vaisband, R. Molkenbur, J. Merrin,
E. Kiermaier, J. Hasenauer, Npj Systems Biology and Applications 12 (2026).
date_created: 2026-02-16T10:44:31Z
date_published: 2026-02-05T00:00:00Z
date_updated: 2026-02-23T10:10:10Z
day: '05'
ddc:
- '570'
department:
- _id: NanoFab
doi: 10.1038/s41540-026-00648-9
external_id:
pmid:
- '41611727'
file:
- access_level: open_access
checksum: 99b2e6bbaaedf45f22e07751948669f5
content_type: application/pdf
creator: dernst
date_created: 2026-02-23T10:09:03Z
date_updated: 2026-02-23T10:09:03Z
file_id: '21346'
file_name: 2026_npjSysBioApp_Arruda.pdf
file_size: 10217687
relation: main_file
success: 1
file_date_updated: 2026-02-23T10:09:03Z
has_accepted_license: '1'
intvolume: ' 12'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
pmid: 1
publication: npj Systems Biology and Applications
publication_identifier:
eissn:
- 2056-7189
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Simulation-based inference of cell migration dynamics in complex spatial environments
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 12
year: '2026'
...
---
_id: '21442'
author:
- first_name: Alois
full_name: Schlögl, Alois
id: 45BF87EE-F248-11E8-B48F-1D18A9856A87
last_name: Schlögl
orcid: 0000-0002-5621-8100
citation:
ama: Schlögl A. CA3Simu v1.06 (vargas2026v1). 2026. doi:10.15479/AT-ISTA-21442
apa: Schlögl, A. (2026). CA3Simu v1.06 (vargas2026v1). Institute of Science and
Technology Austria. https://doi.org/10.15479/AT-ISTA-21442
chicago: Schlögl, Alois. “CA3Simu v1.06 (Vargas2026v1).” Institute of Science and
Technology Austria, 2026. https://doi.org/10.15479/AT-ISTA-21442.
ieee: A. Schlögl, “CA3Simu v1.06 (vargas2026v1).” Institute of Science and Technology
Austria, 2026.
ista: Schlögl A. 2026. CA3Simu v1.06 (vargas2026v1), Institute of Science and Technology
Austria, 10.15479/AT-ISTA-21442.
mla: Schlögl, Alois. CA3Simu v1.06 (Vargas2026v1). Institute of Science and
Technology Austria, 2026, doi:10.15479/AT-ISTA-21442.
short: A. Schlögl, (2026).
corr_author: '1'
date_created: 2026-03-12T08:20:46Z
date_published: 2026-03-12T00:00:00Z
date_updated: 2026-03-12T11:28:52Z
day: '12'
department:
- _id: ScienComp
- _id: PeJo
doi: 10.15479/AT-ISTA-21442
ec_funded: 1
file:
- access_level: open_access
checksum: 441c8827717dcda05f91c127d15cf1e9
content_type: application/gzip
creator: schloegl
date_created: 2026-03-12T08:19:14Z
date_updated: 2026-03-12T08:19:14Z
file_id: '21443'
file_name: ca3simu-vargas2026v1.tar.gz
file_size: 160410
relation: main_file
success: 1
- access_level: open_access
checksum: 3c0092076228a15c0a7ae703192d43ea
content_type: text/markdown
creator: schloegl
date_created: 2026-03-12T10:24:45Z
date_updated: 2026-03-12T10:24:45Z
file_id: '21445'
file_name: README.md
file_size: 10923
relation: main_file
success: 1
file_date_updated: 2026-03-12T10:24:45Z
has_accepted_license: '1'
keyword:
- hypocampus
- ca3 simulations
- modelling
license: https://opensource.org/licenses/GPL-3.0
month: '03'
oa: 1
project:
- _id: e62b56fe-ab3c-11f0-94c7-d181dd352b3b
grant_number: '101199096'
name: Synaptic mechanisms of engram storage and retrieval in CA3 hippocampal microcircuits
- _id: bd88be38-d553-11ed-ba76-81d5a70a6ef5
grant_number: P36232
name: Mechanisms of GABA release in hippocampal circuits
- _id: 8d9195e9-16d5-11f0-9cad-d075be887a1e
grant_number: PAT 4178023
name: Synaptic networks of human brain
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '692692'
name: Biophysics and circuit function of a giant cortical glutamatergic synapse
publisher: Institute of Science and Technology Austria
status: public
title: CA3Simu v1.06 (vargas2026v1)
tmp:
legal_code_url: https://www.gnu.org/licenses/gpl-3.0.en.html
name: GNU General Public License 3.0
short: GPL 3.0
type: software
user_id: 68b8ca59-c5b3-11ee-8790-cd641c68093d
year: '2026'
...
---
OA_place: publisher
OA_type: hybrid
_id: '21767'
abstract:
- lang: eng
text: 'The involvement of non-scientific staff in discussions about animal welfare
and scientific quality is essential for biomedical research progress. In this
study, we developed a survey to collect the self-perception of animal care staff
(ACS) and laboratory technicians about their involvement in scientific planning
and conduct. Participants were contacted to complete an anonymous online questionnaire.
We obtained 850 responses, mainly from Europe: 564 from ACS and 286 from laboratory
technicians. Job satisfaction was assessed as positive by ACS and laboratory technicians
despite the low frequency of culture of care activities and mental health meetings.
Both groups expressed their desire to be trained in research planning and conduct;
however, regular training was not reported. In addition, the inability to act
on animal welfare concerns owing to experimental reasons was reported by both
groups. Over half of the participants felt valued and appreciated by the lead
scientists or animal facility manager; however, it is not clear how they are acknowledged,
as their names on the authors list or in the manuscript acknowledgments are barely
included. Our results indicated that involvement of ACS and laboratory technicians
in planning and conducting studies would improve their understanding of how experiments
are done, and therefore communication processes, work satisfaction, animal welfare,
and scientific quality. Finally, we provided recommendations to improve the engagement
of ACS and laboratory technicians in discussions about animal research planning
and conduct.'
- lang: fre
text: 'La participation de personnel non-scientifique aux discussions sur le bien-être
animal et la qualité scientifique est essentielle aux progrès la recherche biomédicale.
Dans cette étude, nous avons développé une enquête pour recueillir l''auto-perception
du personnel chargé des soins prodigués aux animaux (PCSA) et des techniciens
de laboratoire (TL) sur leur implication dans la planification et la conduite
scientifiques. Les participants ont été contactés pour remplir un questionnaire
anonyme en ligne. Nous avons obtenu 850 réponses, principalement en Europe : 564
provenant de PCSA et 286 de TL. La satisfaction au travail a été évaluée comme
positive par le PCSA et les TL malgré la faible fréquence d’activités sur la culture
des soins et de réunions concernant la santé mentale. Bien que les deux groupes
aient exprimé leur désir d''être formés à la planification et à la conduite de
la recherche, aucune formation réelle régulière n''a été signalée. De plus, l''incapacité
d''agir sur les préoccupations relatives au bien-être animal pour des raisons
expérimentales a été signalée par les deux groupes. Plus de la moitié des participants
se sont sentis valorisés et appréciés par les scientifiques principaux ou le gestionnaire
de l’installation animale mais on ne sait pas clairement comment ils sont reconnus,
car leurs noms sur la liste des auteurs ou dans les remerciements sont à peine
inclus dans la documentation. Nos résultats ont indiqué que la participation du
PCSA et des TL à la planification et à la conduite des études améliorerait leur
compréhension de la façon dont les expériences sont effectuées et, par conséquent,
les processus de communication, leur satisfaction au travail ainsi que le bien-être
animal et la qualité scientifique. Enfin, nous avons formulé des recommandations
pour améliorer la participation du PCSA et des TL aux discussions sur la planification
et la conduite de la recherche animale.'
- lang: ger
text: 'Die Einbeziehung von nichtwissenschaftlichem Personal in Diskussionen über
Tierschutz und wissenschaftliche Qualität ist für Fortschritte in biomedizinischer
Forschung von entscheidender Bedeutung. In dieser Studie haben wir eine Umfrage
entwickelt, um die Selbsteinschätzung von Tierpflegern (ACS) und Labortechnikern
(LT) hinsichtlich ihrer Beteiligung an der wissenschaftlichen Planung und Durchführung
zu erfassen. Die Teilnehmer wurden gebeten, einen anonymen Online-Fragebogen auszufüllen.
Wir erhielten 850 Rückmeldungen, hauptsächlich aus Europa: 564 von ACS und 286
von LT. Die Arbeitszufriedenheit wurde von ACS und LT trotz der geringen Häufigkeit
von Pflegeaktivitäten und Treffen zum Thema psychische Gesundheit als positiv
bewertet. Beide Gruppen äußerten den Wunsch, in der Forschungsplanung und -durchführung
geschult zu werden, doch regelmäßig stattfindende Schulungen wurden nicht berichtet.
Außerdem wurde von beiden Gruppen vermeldet, dass sie aus versuchstechnischen
Gründen nicht in der Lage waren, auf Tierschutzbedenken zu reagieren. Über die
Hälfte der Teilnehmer fühlte sich von den leitenden Wissenschaftlern oder dem
Leiter der Tierhaltungseinrichtung geschätzt und anerkannt; es ist jedoch unklar,
inwiefern sie wirklich gewürdigt werden, da ihre Namen kaum in der Autorenliste
oder in den Danksagungen des Manuskripts aufgeführt sind. Unsere Ergebnisse deuteten
darauf hin, dass die Einbeziehung von ACS und LT in die Planung und Durchführung
von Studien ihr Verständnis für die Durchführung von Experimenten verbessern würde
– und damit auch Kommunikationsprozesse, Arbeitszufriedenheit, Tierwohl und wissenschaftliche
Qualität. Abschließend gaben wir Empfehlungen zur Verbesserung der Einbeziehung
von ACS und LT in Diskussionen über die Planung und Durchführung von Tierversuchen.'
- lang: spa
text: 'La participación del personal no científico en los debates sobre el bienestar
animal y la calidad científica es fundamental para el avance de la investigación
biomédica. En este estudio, desarrollamos una encuesta para recoger la autopercepción
del personal encargado del cuidado de los animales (ACS) y de los técnicos de
laboratorio (LT) sobre su implicación en la planificación y la realización científicas.
Se contactó con los participantes para que cumplimentaran un cuestionario anónimo
en línea. Obtuvimos 850 respuestas, principalmente de Europa: 564 de ACS y 286
de LT. La satisfacción laboral fue evaluada como positiva por ACS y técnicos de
laboratorio a pesar de la baja frecuencia de actividades de cultura del cuidado
y reuniones sobre bienestar mental. Ambos grupos expresaron su deseo de recibir
formación en planificación y realización de investigaciones, sin embargo, no se
informó sobre una formación regular. Asimismo, ambos grupos señalaron la incapacidad
de actuar ante las preocupaciones sobre el bienestar animal por motivos experimentales.
Más de la mitad de los participantes se sintieron valorados y apreciados por los
científicos principales o el responsable de las instalaciones de animales; sin
embargo, no está claro cómo se les reconoce, ya que apenas se incluyen sus nombres
en la lista de autores o en los agradecimientos del manuscrito. Nuestros resultados
indicaron que la participación de los ACS y los LT en la planificación y realización
de los estudios mejoraría su comprensión de cómo se hacen los experimentos y,
por tanto, los procesos de comunicación, la satisfacción laboral, el bienestar
animal y la calidad científica. Finalmente, proporcionamos recomendaciones para
mejorar el compromiso de la AEC y la LT en los debates sobre la planificación
y la realización de investigaciones con animales.'
acknowledgement: "We deeply acknowledge all the animal care staff and laboratory technicians
who participated in this study! We acknowledge Working Groups 1 and 4 from COST
Action IMPROVE (“3Rs concepts to improve the quality of biomedical science”), CA21139,
supported by COST (European Cooperation in Science and Technology) for their feedback
and support. We also acknowledge Aoife Milford for her comments and contributions
to the final draft of the manuscript.\r\nThis publication was based on work from
the COST Action IMPROVE (“3Rs concepts to improve the quality of biomedical science”),
CA21139, supported by COST (European Cooperation in Science and Technology)."
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Fernando
full_name: Gonzalez-Uarquin, Fernando
last_name: Gonzalez-Uarquin
- first_name: Paulin
full_name: Jirkof, Paulin
last_name: Jirkof
- first_name: Bettina
full_name: Bert, Bettina
last_name: Bert
- first_name: Penny
full_name: Hawkins, Penny
last_name: Hawkins
- first_name: Ljupco
full_name: Angelovski, Ljupco
last_name: Angelovski
- first_name: Jan
full_name: Baumgart, Jan
last_name: Baumgart
- first_name: Nadine
full_name: Baumgart, Nadine
last_name: Baumgart
- first_name: Özge S.
full_name: Cevik, Özge S.
last_name: Cevik
- first_name: Nuno H.
full_name: Franco, Nuno H.
last_name: Franco
- first_name: Erdal
full_name: Horata, Erdal
last_name: Horata
- first_name: Rohish
full_name: Kaura, Rohish
last_name: Kaura
- first_name: Winfried
full_name: Neuhaus, Winfried
last_name: Neuhaus
- first_name: Brigida
full_name: Riso, Brigida
last_name: Riso
- first_name: Adrian J.
full_name: Smith, Adrian J.
last_name: Smith
- first_name: Athanassia
full_name: Sotiropoulos, Athanassia
last_name: Sotiropoulos
- first_name: Augusto
full_name: Vitale, Augusto
last_name: Vitale
- first_name: Sophie
full_name: Schober, Sophie
id: 80b0a0ef-4b9f-11ec-b119-8d9d94c4a1d8
last_name: Schober
citation:
ama: 'Gonzalez-Uarquin F, Jirkof P, Bert B, et al. Building bridges: Involvement
of animal care staff and laboratory technicians in experimental planning and conduct
of animal studies for better job satisfaction and science. Laboratory Animals.
2026. doi:10.1177/00236772251400976'
apa: 'Gonzalez-Uarquin, F., Jirkof, P., Bert, B., Hawkins, P., Angelovski, L., Baumgart,
J., … Schober, S. (2026). Building bridges: Involvement of animal care staff and
laboratory technicians in experimental planning and conduct of animal studies
for better job satisfaction and science. Laboratory Animals. SAGE Publications.
https://doi.org/10.1177/00236772251400976'
chicago: 'Gonzalez-Uarquin, Fernando, Paulin Jirkof, Bettina Bert, Penny Hawkins,
Ljupco Angelovski, Jan Baumgart, Nadine Baumgart, et al. “Building Bridges: Involvement
of Animal Care Staff and Laboratory Technicians in Experimental Planning and Conduct
of Animal Studies for Better Job Satisfaction and Science.” Laboratory Animals.
SAGE Publications, 2026. https://doi.org/10.1177/00236772251400976.'
ieee: 'F. Gonzalez-Uarquin et al., “Building bridges: Involvement of animal
care staff and laboratory technicians in experimental planning and conduct of
animal studies for better job satisfaction and science,” Laboratory Animals.
SAGE Publications, 2026.'
ista: 'Gonzalez-Uarquin F, Jirkof P, Bert B, Hawkins P, Angelovski L, Baumgart J,
Baumgart N, Cevik ÖS, Franco NH, Horata E, Kaura R, Neuhaus W, Riso B, Smith AJ,
Sotiropoulos A, Vitale A, Schober S. 2026. Building bridges: Involvement of animal
care staff and laboratory technicians in experimental planning and conduct of
animal studies for better job satisfaction and science. Laboratory Animals.'
mla: 'Gonzalez-Uarquin, Fernando, et al. “Building Bridges: Involvement of Animal
Care Staff and Laboratory Technicians in Experimental Planning and Conduct of
Animal Studies for Better Job Satisfaction and Science.” Laboratory Animals,
SAGE Publications, 2026, doi:10.1177/00236772251400976.'
short: F. Gonzalez-Uarquin, P. Jirkof, B. Bert, P. Hawkins, L. Angelovski, J. Baumgart,
N. Baumgart, Ö.S. Cevik, N.H. Franco, E. Horata, R. Kaura, W. Neuhaus, B. Riso,
A.J. Smith, A. Sotiropoulos, A. Vitale, S. Schober, Laboratory Animals (2026).
date_created: 2026-04-26T22:01:47Z
date_published: 2026-04-14T00:00:00Z
date_updated: 2026-04-28T12:22:17Z
day: '14'
department:
- _id: PreCl
doi: 10.1177/00236772251400976
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1177/00236772251400976
month: '04'
oa: 1
oa_version: Published Version
publication: Laboratory Animals
publication_identifier:
eissn:
- 1758-1117
issn:
- 0023-6772
publication_status: epub_ahead
publisher: SAGE Publications
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Building bridges: Involvement of animal care staff and laboratory technicians
in experimental planning and conduct of animal studies for better job satisfaction
and science'
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2026'
...
---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '21485'
abstract:
- lang: eng
text: Insulating oxides are among the most abundant solid materials in the universe1,2,3.
Of the many ways in which they influence natural phenomena, perhaps the most consequential
is their capacity to transfer electrical charge during contact4,5,6,7,8,9,10—which
occurs even between samples of the same oxide—yet the symmetry-breaking parameter
that causes this remains unidentified11,12. Here we show that adventitious carbonaceous
molecules adsorbed from the environment are the symmetry-breaking factor in same-material
oxide contact electrification (CE). We use acoustic levitation to measure charge
exchange between a sphere and a plate composed of identical amorphous silicon
dioxide (SiO2). Although charging polarity is random for co-prepared samples,
we control it with baking or plasma treatment. Observing the charge-exchange relaxation
afterwards, we see dynamics over a timescale of hours and connect this directly
to the presence of adventitious carbon with time-of-flight mass spectrometry,
low-energy ion scattering and infrared spectroscopy. Going further, we confirm
that adventitious carbon can even determine charge exchange among different oxides.
Our results identify the symmetry-breaking parameter that causes insulating oxides
to exchange charge in settings ranging from desert sands4 to volcanic plumes5,6,
while simultaneously highlighting an overlooked factor in CE more broadly.
acknowledged_ssus:
- _id: M-Shop
- _id: NanoFab
- _id: ScienComp
- _id: LifeSc
acknowledgement: This project has received support from the European Research Council
(ERC) under the European Union’s Horizon 2020 research and innovation programme
(grant agreement no. 949120) and from the Marie Skłodowska-Curie programme (grant
agreement no. 754411). We acknowledge the state of Lower Austria and the European
Regional Development Fund under grant no. WST3-F-542638/004-2021. N.M. acknowledges
support from grant Fondecyt 1221597. G.G. is a Serra Húnter fellow. This research
was supported by the Scientific Service Units of the Institute of Science and Technology
Austria through resources provided by the Miba Machine Shop, Nanofabrication Facility,
Scientific Computing facility and Lab Support Facility. We thank the Modic group
for the use of the Laue camera, T. Zauner for the photography of the experimental
set-up and R. Möller for insightful discussions. Open access funding provided by
Institute of Science and Technology (IST Austria).
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Galien M
full_name: Grosjean, Galien M
id: 0C5FDA4A-9CF6-11E9-8939-FF05E6697425
last_name: Grosjean
orcid: 0000-0001-5154-417X
- first_name: Markus
full_name: Ostermann, Markus
last_name: Ostermann
- first_name: Markus
full_name: Sauer, Markus
last_name: Sauer
- first_name: Michael
full_name: Hahn, Michael
last_name: Hahn
- first_name: Christian M.
full_name: Pichler, Christian M.
last_name: Pichler
- first_name: Florian
full_name: Fahrnberger, Florian
last_name: Fahrnberger
- first_name: Felix
full_name: Pertl, Felix
id: 6313aec0-15b2-11ec-abd3-ed67d16139af
last_name: Pertl
orcid: 0000-0003-0463-5794
- first_name: Daniel
full_name: Balazs, Daniel
id: 302BADF6-85FC-11EA-9E3B-B9493DDC885E
last_name: Balazs
orcid: 0000-0001-7597-043X
- first_name: Mason M.
full_name: Link, Mason M.
last_name: Link
- first_name: Seong H.
full_name: Kim, Seong H.
last_name: Kim
- first_name: Devin L.
full_name: Schrader, Devin L.
last_name: Schrader
- first_name: Adriana
full_name: Blanco, Adriana
last_name: Blanco
- first_name: Francisco
full_name: Gracia, Francisco
last_name: Gracia
- first_name: Nicolás
full_name: Mujica, Nicolás
last_name: Mujica
- first_name: Scott R
full_name: Waitukaitis, Scott R
id: 3A1FFC16-F248-11E8-B48F-1D18A9856A87
last_name: Waitukaitis
orcid: 0000-0002-2299-3176
citation:
ama: Grosjean GM, Ostermann M, Sauer M, et al. Adventitious carbon breaks symmetry
in oxide contact electrification. Nature. 2026;651(8106):626-631. doi:10.1038/s41586-025-10088-w
apa: Grosjean, G. M., Ostermann, M., Sauer, M., Hahn, M., Pichler, C. M., Fahrnberger,
F., … Waitukaitis, S. R. (2026). Adventitious carbon breaks symmetry in oxide
contact electrification. Nature. Springer Nature. https://doi.org/10.1038/s41586-025-10088-w
chicago: Grosjean, Galien M, Markus Ostermann, Markus Sauer, Michael Hahn, Christian
M. Pichler, Florian Fahrnberger, Felix Pertl, et al. “Adventitious Carbon Breaks
Symmetry in Oxide Contact Electrification.” Nature. Springer Nature, 2026.
https://doi.org/10.1038/s41586-025-10088-w.
ieee: G. M. Grosjean et al., “Adventitious carbon breaks symmetry in oxide
contact electrification,” Nature, vol. 651, no. 8106. Springer Nature,
pp. 626–631, 2026.
ista: Grosjean GM, Ostermann M, Sauer M, Hahn M, Pichler CM, Fahrnberger F, Pertl
F, Balazs D, Link MM, Kim SH, Schrader DL, Blanco A, Gracia F, Mujica N, Waitukaitis
SR. 2026. Adventitious carbon breaks symmetry in oxide contact electrification.
Nature. 651(8106), 626–631.
mla: Grosjean, Galien M., et al. “Adventitious Carbon Breaks Symmetry in Oxide Contact
Electrification.” Nature, vol. 651, no. 8106, Springer Nature, 2026, pp.
626–31, doi:10.1038/s41586-025-10088-w.
short: G.M. Grosjean, M. Ostermann, M. Sauer, M. Hahn, C.M. Pichler, F. Fahrnberger,
F. Pertl, D. Balazs, M.M. Link, S.H. Kim, D.L. Schrader, A. Blanco, F. Gracia,
N. Mujica, S.R. Waitukaitis, Nature 651 (2026) 626–631.
corr_author: '1'
date_created: 2026-03-23T15:04:00Z
date_published: 2026-03-18T00:00:00Z
date_updated: 2026-04-28T12:06:01Z
day: '18'
ddc:
- '540'
department:
- _id: ScWa
- _id: GradSch
- _id: LifeSc
doi: 10.1038/s41586-025-10088-w
ec_funded: 1
external_id:
pmid:
- '41851325'
file:
- access_level: open_access
checksum: dafef9ed575b44be4263e948a47ae056
content_type: application/pdf
creator: dernst
date_created: 2026-03-24T06:57:08Z
date_updated: 2026-03-24T06:57:08Z
file_id: '21494'
file_name: 2026_Nature_Grosjean.pdf
file_size: 12245694
relation: main_file
success: 1
file_date_updated: 2026-03-24T06:57:08Z
has_accepted_license: '1'
intvolume: ' 651'
issue: '8106'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
page: 626-631
pmid: 1
project:
- _id: 0aa60e99-070f-11eb-9043-a6de6bdc3afa
call_identifier: H2020
grant_number: '949120'
name: 'Tribocharge: a multi-scale approach to an enduring problem in physics'
- _id: 260C2330-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '754411'
name: ISTplus - Postdoctoral Fellowships
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
- 0028-0836
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- description: News on ISTA website
relation: press_release
url: https://ista.ac.at/en/news/colliding-dust-and-the-sparks-of-creation/
status: public
title: Adventitious carbon breaks symmetry in oxide contact electrification
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
volume: 651
year: '2026'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
PlanS_conform: '1'
_id: '21382'
abstract:
- lang: eng
text: The exceptional energy-harvesting efficiency of lead-halide perovskites arises
from unusually long photocarrier diffusion lengths and recombination lifetimes
that persist even in defect-rich, solution-grown samples. Paradoxically, perovskites
are also known for having very short exciton decay times. Here, we resolve this
apparent contradiction by showing that key optoelectronic properties of perovskites
can be explained by localized flexoelectric polarization confined to interfaces
between domains of spontaneous strain. Using birefringence imaging, electrochemical
staining, and zero-bias photocurrent measurements, we visualize the domain structure
and directly probe the associated internal fields in nominally cubic single crystals
of methylammonium lead bromide. We demonstrate that localized flexoelectric fields
spatially separate electrons and holes to opposite sides of domain walls, exponentially
suppressing recombination. Domain walls thus act as efficient mesoscopic transport
channels for long-lived photocarriers, microscopically linking structural heterogeneity
to charge transport and offering mechanistically informed design principles for
perovskite solar-energy technologies.
acknowledged_ssus:
- _id: Bio
- _id: M-Shop
acknowledgement: We are grateful to A. G. Volosniev for the valuable discussions.
We thank D. Milius for the assistance with microscopy. D. R. would like to thank
F. Filakovský and T. Čuchráč for the valuable discussions. This research was supported
by the Scientific Service Units (SSU) of ISTA through resources provided by the
Imaging & Optics Facility (IOF) and the Miba Machine Shop Facility (MS).
article_number: '946'
article_processing_charge: Yes
article_type: original
author:
- first_name: Dmytro
full_name: Rak, Dmytro
id: 70313b46-47c2-11ec-9e88-cd79101918fe
last_name: Rak
- first_name: Dusan
full_name: Lorenc, Dusan
id: 40D8A3E6-F248-11E8-B48F-1D18A9856A87
last_name: Lorenc
- first_name: Daniel
full_name: Balazs, Daniel
id: 302BADF6-85FC-11EA-9E3B-B9493DDC885E
last_name: Balazs
orcid: 0000-0001-7597-043X
- first_name: Ayan A.
full_name: Zhumekenov, Ayan A.
last_name: Zhumekenov
- first_name: Osman M.
full_name: Bakr, Osman M.
last_name: Bakr
- first_name: Zhanybek
full_name: Alpichshev, Zhanybek
id: 45E67A2A-F248-11E8-B48F-1D18A9856A87
last_name: Alpichshev
orcid: 0000-0002-7183-5203
citation:
ama: Rak D, Lorenc D, Balazs D, Zhumekenov AA, Bakr OM, Alpichshev Z. Flexoelectric
domain walls enable charge separation and transport in cubic perovskites. Nature
Communications. 2026;17. doi:10.1038/s41467-026-68660-5
apa: Rak, D., Lorenc, D., Balazs, D., Zhumekenov, A. A., Bakr, O. M., & Alpichshev,
Z. (2026). Flexoelectric domain walls enable charge separation and transport in
cubic perovskites. Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-026-68660-5
chicago: Rak, Dmytro, Dusan Lorenc, Daniel Balazs, Ayan A. Zhumekenov, Osman M.
Bakr, and Zhanybek Alpichshev. “Flexoelectric Domain Walls Enable Charge Separation
and Transport in Cubic Perovskites.” Nature Communications. Springer Nature,
2026. https://doi.org/10.1038/s41467-026-68660-5.
ieee: D. Rak, D. Lorenc, D. Balazs, A. A. Zhumekenov, O. M. Bakr, and Z. Alpichshev,
“Flexoelectric domain walls enable charge separation and transport in cubic perovskites,”
Nature Communications, vol. 17. Springer Nature, 2026.
ista: Rak D, Lorenc D, Balazs D, Zhumekenov AA, Bakr OM, Alpichshev Z. 2026. Flexoelectric
domain walls enable charge separation and transport in cubic perovskites. Nature
Communications. 17, 946.
mla: Rak, Dmytro, et al. “Flexoelectric Domain Walls Enable Charge Separation and
Transport in Cubic Perovskites.” Nature Communications, vol. 17, 946, Springer
Nature, 2026, doi:10.1038/s41467-026-68660-5.
short: D. Rak, D. Lorenc, D. Balazs, A.A. Zhumekenov, O.M. Bakr, Z. Alpichshev,
Nature Communications 17 (2026).
corr_author: '1'
date_created: 2026-03-02T10:06:58Z
date_published: 2026-02-16T00:00:00Z
date_updated: 2026-04-28T12:12:46Z
day: '16'
ddc:
- '530'
department:
- _id: ZhAl
- _id: LifeSc
doi: 10.1038/s41467-026-68660-5
external_id:
pmid:
- '41698893'
file:
- access_level: open_access
checksum: dd7a98de892d0b5abefca7e290ca0f77
content_type: application/pdf
creator: dernst
date_created: 2026-03-02T14:27:56Z
date_updated: 2026-03-02T14:27:56Z
file_id: '21390'
file_name: 2026_NatureComm_Rak.pdf
file_size: 2570918
relation: main_file
success: 1
file_date_updated: 2026-03-02T14:27:56Z
has_accepted_license: '1'
intvolume: ' 17'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
pmid: 1
publication: Nature Communications
publication_identifier:
eissn:
- 2041-1723
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- description: News on ISTA website
relation: press_release
url: https://ista.ac.at/en/news/explaining-next-generation-solar-cells/
scopus_import: '1'
status: public
title: Flexoelectric domain walls enable charge separation and transport in cubic
perovskites
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
volume: 17
year: '2026'
...
---
OA_type: closed access
_id: '21762'
abstract:
- lang: eng
text: Bacteria, like eukaryotes, use conserved cytoskeletal systems for intracellular
organization. The plasmid-encoded ParMRC system forms actin-like filaments that
segregate low–copy number plasmids. In multicellular cyanobacteria such as Anabaena
sp., we found that a chromosomally encoded ParMR system has evolved into a cytoskeletal
system named CorMR with a function in cell shape control rather than DNA segregation.
Live-cell imaging, in vitro reconstitution, and cryo–electron microscopy revealed
that CorM formed dynamically unstable, antiparallel double-stranded filaments
that were recruited to the membrane by CorR through an amphipathic helix conserved
in multicellular cyanobacteria. CorMR filaments were regulated by MinC, which
excluded them from the poles and division plane. Comparative genomics indicated
that the repurposing of ParMR and Min systems coevolved with cyanobacterial multicellularity,
highlighting the evolutionary plasticity of cytoskeletal systems in bacteria.
acknowledged_ssus:
- _id: Bio
- _id: ScienComp
- _id: EM-Fac
- _id: LifeSc
acknowledgement: "We thank all members of the Loose lab at ISTA for helpful discussions;
M. Kojic for critical reading of the manuscript; A. Herrero (Sevilla University)
for sharing her extensive BACTH plasmid library and other plasmids, as well as cyanobacterial
strains; T. Dagan and F. Nies (both Kiel University) for sharing cyanobacterial
strains and plasmids and for valuable discussions; N. Sapay and A. Michon for providing
the Amphipaseek code, which enabled us to perform our large-scale amphipathic helix
screen of cyanobacterial CorR proteins; V.-V. Hodirnau for support in cryo-ET data
collection; and J. Hansen for advice about cryo-EM data processing.\r\nThis work
was supported by the Scientific Service Units (SSU) of ISTA through resources provided
by the Imaging & Optics Facility (IOF), the Scientific Computing (SciComp), the
Electron Microscopy Facility (EMF), and the Lab Support Facility (LSF). This work
was funded by the European Union’s Horizon 2020 research and innovation program
(Marie Skłodowska-Curie grant 101034413 to B.L.S.); the European Research Council
(ERC) of the European Union (grant ActinID 101076260 to F.K.M.S.); the Swiss National
Science Foundation (starting grant TMSGI3_226208 to G.L.W.); and the Jean-Jacques
et Letitia Lopez-Loreta Foundation (G.L.W.)."
article_number: eaea6343
article_processing_charge: No
article_type: original
author:
- first_name: Benjamin L
full_name: Springstein, Benjamin L
id: b4eb62ef-ac72-11ed-9503-ed3b4d66c083
last_name: Springstein
orcid: 0000-0002-3461-5391
- first_name: Manjunath
full_name: Javoor, Manjunath
id: 305ab18b-dc7d-11ea-9b2f-b58195228ea2
last_name: Javoor
orcid: 0000-0003-2311-2112
- first_name: Daniela
full_name: Megrian, Daniela
last_name: Megrian
- first_name: Roman
full_name: Hajdu, Roman
id: ffab949d-133f-11ed-8f02-94de21ace503
last_name: Hajdu
- first_name: Dustin M.
full_name: Hanke, Dustin M.
last_name: Hanke
- first_name: Bettina
full_name: Zens, Bettina
id: 45FD126C-F248-11E8-B48F-1D18A9856A87
last_name: Zens
orcid: 0000-0002-9561-1239
- first_name: Gregor L.
full_name: Weiss, Gregor L.
last_name: Weiss
- first_name: Florian Km
full_name: Schur, Florian Km
id: 48AD8942-F248-11E8-B48F-1D18A9856A87
last_name: Schur
orcid: 0000-0003-4790-8078
- first_name: Martin
full_name: Loose, Martin
id: 462D4284-F248-11E8-B48F-1D18A9856A87
last_name: Loose
orcid: 0000-0001-7309-9724
citation:
ama: Springstein BL, Javoor M, Megrian D, et al. Repurposing of a DNA segregation
machinery into a cytoskeletal system controlling cell shape. Science. 2026;392(6795).
doi:10.1126/science.aea6343
apa: Springstein, B. L., Javoor, M., Megrian, D., Hajdu, R., Hanke, D. M., Zens,
B., … Loose, M. (2026). Repurposing of a DNA segregation machinery into a cytoskeletal
system controlling cell shape. Science. AAAS. https://doi.org/10.1126/science.aea6343
chicago: Springstein, Benjamin L, Manjunath Javoor, Daniela Megrian, Roman Hajdu,
Dustin M. Hanke, Bettina Zens, Gregor L. Weiss, Florian KM Schur, and Martin Loose.
“Repurposing of a DNA Segregation Machinery into a Cytoskeletal System Controlling
Cell Shape.” Science. AAAS, 2026. https://doi.org/10.1126/science.aea6343.
ieee: B. L. Springstein et al., “Repurposing of a DNA segregation machinery
into a cytoskeletal system controlling cell shape,” Science, vol. 392,
no. 6795. AAAS, 2026.
ista: Springstein BL, Javoor M, Megrian D, Hajdu R, Hanke DM, Zens B, Weiss GL,
Schur FK, Loose M. 2026. Repurposing of a DNA segregation machinery into a cytoskeletal
system controlling cell shape. Science. 392(6795), eaea6343.
mla: Springstein, Benjamin L., et al. “Repurposing of a DNA Segregation Machinery
into a Cytoskeletal System Controlling Cell Shape.” Science, vol. 392,
no. 6795, eaea6343, AAAS, 2026, doi:10.1126/science.aea6343.
short: B.L. Springstein, M. Javoor, D. Megrian, R. Hajdu, D.M. Hanke, B. Zens, G.L.
Weiss, F.K. Schur, M. Loose, Science 392 (2026).
corr_author: '1'
date_created: 2026-04-26T22:01:46Z
date_published: 2026-04-16T00:00:00Z
date_updated: 2026-04-28T13:29:05Z
day: '16'
department:
- _id: MaLo
- _id: FlSc
- _id: GradSch
- _id: EM-Fac
doi: 10.1126/science.aea6343
ec_funded: 1
external_id:
pmid:
- '41990175'
intvolume: ' 392'
issue: '6795'
language:
- iso: eng
month: '04'
oa_version: None
pmid: 1
project:
- _id: fc2ed2f7-9c52-11eb-aca3-c01059dda49c
call_identifier: H2020
grant_number: '101034413'
name: 'IST-BRIDGE: International postdoctoral program'
- _id: bd980d18-d553-11ed-ba76-ceaa645c97eb
grant_number: '101076260'
name: A molecular atlas of Actin filament IDentities in the cell motility machinery
publication: Science
publication_identifier:
eissn:
- 1095-9203
issn:
- 0036-8075
publication_status: published
publisher: AAAS
quality_controlled: '1'
scopus_import: '1'
status: public
title: Repurposing of a DNA segregation machinery into a cytoskeletal system controlling
cell shape
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 392
year: '2026'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
PlanS_conform: '1'
_id: '21711'
abstract:
- lang: eng
text: 'Background: Low-volume trapping columns are essential for sample enrichment,
desalting, and injection profile focusing on nano-LC–MS-based proteomics. They
enable higher sample loading, improve chromatographic performance, and protect
the analytical column by removing salts and contaminants. Recently, monolithic
trap columns with micropillar architecture have emerged as alternatives to conventionally
packed traps. This study compares the performance of a packed and a micropillar
monolithic trap column for the analysis of tryptic peptides. Methods: A tryptic
digest of HeLa cell lysate was analyzed under identical LC–MS conditions using
both trap types. Peptides were detected at 214 nm and analyzed by nano-ESI on
a Q Exactive Plus Orbitrap. Data were searched against the human UniProt database
(February 2023) using FragPipe v20.0, and statistical evaluation of MaxLFQ intensities
was performed in Perseus using Welch’s t-test and clustering analysis. Results:
Over 2500 proteins were identified with both setups. The packed trap column yielded
more total peptides, particularly those with post-translational modifications
and higher hydrophilicity, whereas the monolithic column favored peptides of intermediate
hydrophobicity. Chromatographic profiles confirmed a slight reduction in the trapping
efficiency of hydrophilic peptides by the monolithic trap. Conclusions: Trap column
design significantly influences peptide recovery and proteome coverage.'
acknowledgement: 'The authors thank Gábor Tóth, Uppsala University, Sweden, and Armel
Nicolas, Institute for Science and Technology Austria, for their support. This research
was conducted during a student residency in Vienna under the auspices of OeAD. ZI:
ICM-2016-03196.'
article_number: '10'
article_processing_charge: Yes
article_type: original
author:
- first_name: Jadranka
full_name: Miletić Vukajlović, Jadranka
last_name: Miletić Vukajlović
- first_name: Bojana
full_name: Ilić, Bojana
last_name: Ilić
- first_name: Bella
full_name: Bruszel, Bella
id: 70abbbb3-88ea-11ec-8e0a-e8c939944834
last_name: Bruszel
- first_name: Tanja
full_name: Panić-Janković, Tanja
last_name: Panić-Janković
- first_name: Goran
full_name: Mitulović, Goran
last_name: Mitulović
citation:
ama: Miletić Vukajlović J, Ilić B, Bruszel B, Panić-Janković T, Mitulović G. Comparison
of the trapping efficiency for tryptic peptides on particle-packed and micro-pillar
trap columns for proteomics analyses. Proteomes. 2026;14(1). doi:10.3390/proteomes14010010
apa: Miletić Vukajlović, J., Ilić, B., Bruszel, B., Panić-Janković, T., & Mitulović,
G. (2026). Comparison of the trapping efficiency for tryptic peptides on particle-packed
and micro-pillar trap columns for proteomics analyses. Proteomes. MDPI.
https://doi.org/10.3390/proteomes14010010
chicago: Miletić Vukajlović, Jadranka, Bojana Ilić, Bella Bruszel, Tanja Panić-Janković,
and Goran Mitulović. “Comparison of the Trapping Efficiency for Tryptic Peptides
on Particle-Packed and Micro-Pillar Trap Columns for Proteomics Analyses.” Proteomes.
MDPI, 2026. https://doi.org/10.3390/proteomes14010010.
ieee: J. Miletić Vukajlović, B. Ilić, B. Bruszel, T. Panić-Janković, and G. Mitulović,
“Comparison of the trapping efficiency for tryptic peptides on particle-packed
and micro-pillar trap columns for proteomics analyses,” Proteomes, vol.
14, no. 1. MDPI, 2026.
ista: Miletić Vukajlović J, Ilić B, Bruszel B, Panić-Janković T, Mitulović G. 2026.
Comparison of the trapping efficiency for tryptic peptides on particle-packed
and micro-pillar trap columns for proteomics analyses. Proteomes. 14(1), 10.
mla: Miletić Vukajlović, Jadranka, et al. “Comparison of the Trapping Efficiency
for Tryptic Peptides on Particle-Packed and Micro-Pillar Trap Columns for Proteomics
Analyses.” Proteomes, vol. 14, no. 1, 10, MDPI, 2026, doi:10.3390/proteomes14010010.
short: J. Miletić Vukajlović, B. Ilić, B. Bruszel, T. Panić-Janković, G. Mitulović,
Proteomes 14 (2026).
date_created: 2026-04-12T22:01:49Z
date_published: 2026-03-01T00:00:00Z
date_updated: 2026-05-04T10:36:21Z
day: '01'
ddc:
- '540'
department:
- _id: MassSpec
doi: 10.3390/proteomes14010010
external_id:
pmid:
- '41893725'
file:
- access_level: open_access
checksum: 1e0c66bbf4b6e0be626a8639ea664b63
content_type: application/pdf
creator: dernst
date_created: 2026-05-04T10:31:35Z
date_updated: 2026-05-04T10:31:35Z
file_id: '21790'
file_name: 2026_Proteomes_Vukajlovic.pdf
file_size: 1009723
relation: main_file
success: 1
file_date_updated: 2026-05-04T10:31:35Z
has_accepted_license: '1'
intvolume: ' 14'
issue: '1'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
pmid: 1
publication: Proteomes
publication_identifier:
eissn:
- 2227-7382
publication_status: published
publisher: MDPI
quality_controlled: '1'
scopus_import: '1'
status: public
title: Comparison of the trapping efficiency for tryptic peptides on particle-packed
and micro-pillar trap columns for proteomics analyses
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 14
year: '2026'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
PlanS_conform: '1'
_id: '21746'
abstract:
- lang: eng
text: As vertebrates transitioned from water to land, locomotion shifted from undulatory
swimming to limb-based movement. How spinal circuits and their cell types evolved
to support this transition remains unclear. We leverage frog metamorphosis, which
recapitulates this transition within a single organism, to define how spinal circuits
generate aquatic versus terrestrial motor patterns. At swim stages, spinal architecture
is uniform, with a transcriptionally and anatomically homogeneous motor and interneurons.
As limbs develop and their movement complexifies, spinal circuits expand in neuron
number and subtype diversity. This expansion is most pronounced for V1 inhibitory
neurons, which increase ∼70-fold and diversify into transcriptionally distinct
subtypes. Disrupting transcription factors defining emerging motor and V1 populations
reveals molecular segregation between swim and limb circuits, highlighting the
role of subtype diversity in motor coordination. A multifold increase in inhibitory
neuron diversity thus underlies the tail-to-limb locomotor transition, providing
a framework for spinal circuit adaptation during vertebrate evolution.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: 'We would like to thank the members of the Sweeney Lab, Mario de
Bono, Michael Forsthofer, Katharina Lust, and Meital Oren, for comments on the manuscript.
We are also grateful to Tom Jessell and Chris Kintner for their scientific insight
and mentorship during the conception of this project. It would also have not been
possible without the technical support of the Aquatics and Imaging and Optics Facility
support teams (ISTA). We thank Martin Estermann for preparing the initial draft
of the graphical abstract and Niki Barolini for the final version. In addition,
we thank our funding sources for providing the resources to do these experiments:
GFF NÖ FTI Strategy Lower Austria dissertation grant FT121-D-046 (to D.V.), Horizon
Europe ERC starting grant 101041551 (to Y.I., L.B.S., F.A.T., and D.V.), Special
Research Program (SFB) of the Austrian Science Fund (FWF) project F7814-B (to L.B.S.),
Austrian Science Fund (FWF) 10.55776/COE16 (to Y.I. and L.B.S.), NINDS 5R35NS116858
(to J.S.D.), CZI grant DAF2020-225401 (DOI) 10.37921/120055ratwvi (to R.H.), NIH
grant R01NS123116 (to J.B.B.), American Lebanese Syrian Associated Charities (ALSAC)
(to J.B.B.), German Academic Exchange Service (DAAD) IFI grant 57515251-91853472
(to Z.H.), and Project A.L.S. (to S.B.-M.).'
article_number: '117227'
article_processing_charge: Yes
article_type: original
author:
- first_name: David
full_name: Vijatovic, David
id: cf391e77-ec3c-11ea-a124-d69323410b58
last_name: Vijatovic
- first_name: 'Florina Alexandra '
full_name: 'Toma, Florina Alexandra '
id: 2f73f876-f128-11eb-9611-b96b5a30cb0e
last_name: Toma
- first_name: Y
full_name: Ignatyev, Y
last_name: Ignatyev
- first_name: Zoe P
full_name: Harrington, Zoe P
id: a8144562-32c9-11ee-b5ce-d9800628bda2
last_name: Harrington
orcid: 0009-0008-0158-4032
- first_name: Christoph M
full_name: Sommer, Christoph M
id: 4DF26D8C-F248-11E8-B48F-1D18A9856A87
last_name: Sommer
orcid: 0000-0003-1216-9105
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Matthijs Geert
full_name: Smits, Matthijs Geert
id: 7a231d52-e216-11ee-a0bb-8acd55f8f1f0
last_name: Smits
- first_name: Marco
full_name: Dalla Vecchia, Marco
id: 02a7a869-ff06-11ed-a87f-86649d6077e5
last_name: Dalla Vecchia
- first_name: Alexandra J.
full_name: Trevisan, Alexandra J.
last_name: Trevisan
- first_name: Phillip
full_name: Chapman, Phillip
last_name: Chapman
- first_name: Mara
full_name: Julseth, Mara
id: 1cf464b2-dc7d-11ea-9b2f-f9b1aa9417d1
last_name: Julseth
- first_name: Susan
full_name: Brenner-Morton, Susan
last_name: Brenner-Morton
- first_name: Mariano I.
full_name: Gabitto, Mariano I.
last_name: Gabitto
- first_name: Jeremy S.
full_name: Dasen, Jeremy S.
last_name: Dasen
- first_name: Jay B.
full_name: Bikoff, Jay B.
last_name: Bikoff
- first_name: Lora Beatrice Jaeger
full_name: Sweeney, Lora Beatrice Jaeger
id: 56BE8254-C4F0-11E9-8E45-0B23E6697425
last_name: Sweeney
orcid: 0000-0001-9242-5601
citation:
ama: Vijatovic D, Toma FA, Ignatyev Y, et al. Multifold increase in spinal inhibitory
cell types with emergence of limb movement. Cell Reports. 2026;45(4). doi:10.1016/j.celrep.2026.117227
apa: Vijatovic, D., Toma, F. A., Ignatyev, Y., Harrington, Z. P., Sommer, C. M.,
Hauschild, R., … Sweeney, L. B. (2026). Multifold increase in spinal inhibitory
cell types with emergence of limb movement. Cell Reports. Elsevier. https://doi.org/10.1016/j.celrep.2026.117227
chicago: Vijatovic, David, Florina Alexandra Toma, Y Ignatyev, Zoe P Harrington,
Christoph M Sommer, Robert Hauschild, Matthijs Geert Smits, et al. “Multifold
Increase in Spinal Inhibitory Cell Types with Emergence of Limb Movement.” Cell
Reports. Elsevier, 2026. https://doi.org/10.1016/j.celrep.2026.117227.
ieee: D. Vijatovic et al., “Multifold increase in spinal inhibitory cell
types with emergence of limb movement,” Cell Reports, vol. 45, no. 4. Elsevier,
2026.
ista: Vijatovic D, Toma FA, Ignatyev Y, Harrington ZP, Sommer CM, Hauschild R, Smits
MG, Dalla Vecchia M, Trevisan AJ, Chapman P, Julseth M, Brenner-Morton S, Gabitto
MI, Dasen JS, Bikoff JB, Sweeney LB. 2026. Multifold increase in spinal inhibitory
cell types with emergence of limb movement. Cell Reports. 45(4), 117227.
mla: Vijatovic, David, et al. “Multifold Increase in Spinal Inhibitory Cell Types
with Emergence of Limb Movement.” Cell Reports, vol. 45, no. 4, 117227,
Elsevier, 2026, doi:10.1016/j.celrep.2026.117227.
short: D. Vijatovic, F.A. Toma, Y. Ignatyev, Z.P. Harrington, C.M. Sommer, R. Hauschild,
M.G. Smits, M. Dalla Vecchia, A.J. Trevisan, P. Chapman, M. Julseth, S. Brenner-Morton,
M.I. Gabitto, J.S. Dasen, J.B. Bikoff, L.B. Sweeney, Cell Reports 45 (2026).
corr_author: '1'
date_created: 2026-04-19T22:07:43Z
date_published: 2026-04-28T00:00:00Z
date_updated: 2026-05-04T12:27:06Z
day: '28'
ddc:
- '570'
department:
- _id: LoSw
- _id: GradSch
- _id: TiVo
- _id: Bio
- _id: NiBa
doi: 10.1016/j.celrep.2026.117227
external_id:
pmid:
- '41964955 '
file:
- access_level: open_access
checksum: 0d26cdb5b8d8dec3a911d8261a65cdef
content_type: application/pdf
creator: dernst
date_created: 2026-05-04T12:20:10Z
date_updated: 2026-05-04T12:20:10Z
file_id: '21795'
file_name: 2026_CellReports_Vijatovic.pdf
file_size: 14925958
relation: main_file
success: 1
file_date_updated: 2026-05-04T12:20:10Z
has_accepted_license: '1'
intvolume: ' 45'
issue: '4'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: ebb66355-77a9-11ec-83b8-b8ac210a4dae
grant_number: '101041551'
name: Development and Evolution of Tetrapod Motor Circuits
- _id: 8da85f50-16d5-11f0-9cad-eab8b0ff6c9e
grant_number: F7814
name: 'Stem Cell Modulation in Neural Development and Regeneration/ P14-Swim-to-limb
transition: cell type to connection diversity'
- _id: c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473
grant_number: CZI01
name: Tools for automation and feedback microscopy
- _id: bd73af52-d553-11ed-ba76-912049f0ac7a
grant_number: FTI21-D-046
name: Development of V1 interneuron diversity during swim-to-walk transition of
Xenopus metamorphosis
publication: Cell Reports
publication_identifier:
eissn:
- 2211-1247
issn:
- 2639-1856
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Multifold increase in spinal inhibitory cell types with emergence of limb movement
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 45
year: '2026'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '21779'
abstract:
- lang: eng
text: Acidomycin is an anti-mycobacterial antibiotic with a unique mode of action,
targeting the biotin biosynthesis pathway. Despite being highly active against
mycobacteria in vitro, its development as an anti-tubercular agent has been hindered
due to suboptimal pharmacokinetics. Engineering of the acidomycin biosynthesis
may yield new analogues with improved pharmacological properties. Here, we describe
the identification of the acidomycin biosynthetic gene cluster (BGC) in a Streptomyces
bacterium isolated from the rhizosphere of Edelweiss. Notably, the acidomycin
BGC is located in proximity to the genes for the biosynthesis of stravidins, secondary
metabolites targeting a different enzyme in the biotin biosynthesis pathway, and
two genes for streptavidins, proteins that strongly bind and sequester biotin.
The identity of the acidomycin BGC was confirmed via both gene knock-out and heterologous
expression, which suggested that the fatty acid required for the formation of
acidomycin's acyl chain is most likely scavenged from the biotin biosynthesis
pathway. CRISPR/Cas9-assisted knock-out of the cytochrome P450-encoding gene in
the acidomycin BGC resulted in a significant decrease in its yield but did not
abrogate the biosynthesis completely.
acknowledgement: This work was supported by the University of Vienna. The authors
thank Anna Fabisikova from the Mass Spectrometry Centre and the team of the NMR
Centre (both of the Faculty of Chemistry, University of Vienna and members of the
Vienna Life Science Instruments) for assistance with data acquisition. Open Access
funding provided by Universitat Wien. This work was supported by Universität Wien.
article_number: e70357
article_processing_charge: Yes
article_type: original
author:
- first_name: Anna
full_name: Vignolle, Anna
last_name: Vignolle
- first_name: Martin
full_name: Zehl, Martin
id: 8e016d5b-5d77-11f0-86d2-96cdb3922a55
last_name: Zehl
orcid: 0000-0001-9685-0373
- first_name: Jaime Felipe Guerrero
full_name: Garzón, Jaime Felipe Guerrero
last_name: Garzón
- first_name: Olha
full_name: Schneider, Olha
last_name: Schneider
- first_name: Johannes
full_name: Gafriller, Johannes
last_name: Gafriller
- first_name: Ulrike
full_name: Grienke, Ulrike
last_name: Grienke
- first_name: Rasmus H.
full_name: Kirkegaard, Rasmus H.
last_name: Kirkegaard
- first_name: Sergey B.
full_name: Zotchev, Sergey B.
last_name: Zotchev
citation:
ama: Vignolle A, Zehl M, Garzón JFG, et al. Identification and characterisation
of the gene cluster governing biosynthesis of the anti-mycobacterial antibiotic
acidomycin. Microbial Biotechnology. 2026;19(4). doi:10.1111/1751-7915.70357
apa: Vignolle, A., Zehl, M., Garzón, J. F. G., Schneider, O., Gafriller, J., Grienke,
U., … Zotchev, S. B. (2026). Identification and characterisation of the gene cluster
governing biosynthesis of the anti-mycobacterial antibiotic acidomycin. Microbial
Biotechnology. Wiley. https://doi.org/10.1111/1751-7915.70357
chicago: Vignolle, Anna, Martin Zehl, Jaime Felipe Guerrero Garzón, Olha Schneider,
Johannes Gafriller, Ulrike Grienke, Rasmus H. Kirkegaard, and Sergey B. Zotchev.
“Identification and Characterisation of the Gene Cluster Governing Biosynthesis
of the Anti-Mycobacterial Antibiotic Acidomycin.” Microbial Biotechnology.
Wiley, 2026. https://doi.org/10.1111/1751-7915.70357.
ieee: A. Vignolle et al., “Identification and characterisation of the gene
cluster governing biosynthesis of the anti-mycobacterial antibiotic acidomycin,”
Microbial Biotechnology, vol. 19, no. 4. Wiley, 2026.
ista: Vignolle A, Zehl M, Garzón JFG, Schneider O, Gafriller J, Grienke U, Kirkegaard
RH, Zotchev SB. 2026. Identification and characterisation of the gene cluster
governing biosynthesis of the anti-mycobacterial antibiotic acidomycin. Microbial
Biotechnology. 19(4), e70357.
mla: Vignolle, Anna, et al. “Identification and Characterisation of the Gene Cluster
Governing Biosynthesis of the Anti-Mycobacterial Antibiotic Acidomycin.” Microbial
Biotechnology, vol. 19, no. 4, e70357, Wiley, 2026, doi:10.1111/1751-7915.70357.
short: A. Vignolle, M. Zehl, J.F.G. Garzón, O. Schneider, J. Gafriller, U. Grienke,
R.H. Kirkegaard, S.B. Zotchev, Microbial Biotechnology 19 (2026).
date_created: 2026-05-03T22:01:37Z
date_published: 2026-04-01T00:00:00Z
date_updated: 2026-05-07T08:22:41Z
day: '01'
ddc:
- '570'
department:
- _id: MassSpec
doi: 10.1111/1751-7915.70357
external_id:
pmid:
- '42036976'
file:
- access_level: open_access
checksum: 8c8aa660cef5394167e06f187adbabf0
content_type: application/pdf
creator: dernst
date_created: 2026-05-07T08:21:06Z
date_updated: 2026-05-07T08:21:06Z
file_id: '21835'
file_name: 2026_MicrobialBiotechnology_Vignolle.pdf
file_size: 575492
relation: main_file
success: 1
file_date_updated: 2026-05-07T08:21:06Z
has_accepted_license: '1'
intvolume: ' 19'
issue: '4'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
publication: Microbial Biotechnology
publication_identifier:
eissn:
- 1751-7915
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Identification and characterisation of the gene cluster governing biosynthesis
of the anti-mycobacterial antibiotic acidomycin
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 19
year: '2026'
...
---
OA_place: repository
OA_type: green
_id: '21848'
abstract:
- lang: eng
text: 'Despite the success of mRNA therapeutics, challenges remain in optimizing
immune responses and minimizing side effects. Cell-specific antigen delivery may
help reduce required doses and improve vaccine efficacy. In this study, we report
on a targeted delivery system for mRNA to a specific subset of skin-resident antigen-presenting
cells: Langerhans cells. By functionalizing lipid nanoparticles with a langerin-specific
glycomimetic ligand, we achieve selective mRNA delivery to both murine and human
primary Langerhans cells with minimal off-target uptake, at the same time resulting
in significantly increased mRNA translation. This targeted mRNA delivery not only
enhances antigen presentation and T-cell responses but also enables dose-sparing
and superior antitumor immunity compared with conventional immunization in a B16-OVA
tumor model. Importantly, our platform’s high compatibility with various lipid
nanoparticle formulations offers a flexible and precise tool for skin-directed
mRNA delivery.'
acknowledged_ssus:
- _id: PreCl
acknowledgement: We thank Mareike Rentzsch for her intellectual contributions during
the course of our discussions. We thank Michael Schunn from the Preclinical Facility
of the Institute of Science and Technology Austria for his continuous technical
support. Guarantor of the work is FS. This project was supported by “Seedfinancing”
(P2282679) of the Austrian Federal Ministry of Digital and Economic Affairs and
the Ministry of Climate Action and Energy, handled by the Austrian Wirtschaftsservice,
as well as by...
article_processing_charge: No
article_type: original
author:
- first_name: Klara
full_name: Klein, Klara
last_name: Klein
- first_name: Litty
full_name: Johnson, Litty
last_name: Johnson
- first_name: Ramona
full_name: Rîca, Ramona
last_name: Rîca
- first_name: Mirza
full_name: Sarcevic, Mirza
last_name: Sarcevic
- first_name: Gabriele
full_name: Carta, Gabriele
last_name: Carta
- first_name: Saskia
full_name: Seiser, Saskia
last_name: Seiser
- first_name: Adelheid
full_name: Elbe-Bürger, Adelheid
last_name: Elbe-Bürger
- first_name: Freyja
full_name: Langer, Freyja
id: 3C1BE782-F248-11E8-B48F-1D18A9856A87
last_name: Langer
- first_name: Nowras
full_name: Rahhal, Nowras
last_name: Rahhal
- first_name: Christoph
full_name: Rademacher, Christoph
last_name: Rademacher
- first_name: Robert
full_name: Wawrzinek, Robert
last_name: Wawrzinek
- first_name: Federica
full_name: Quattrone, Federica
last_name: Quattrone
- first_name: Florian
full_name: Sparber, Florian
last_name: Sparber
citation:
ama: 'Klein K, Johnson L, Rîca R, et al. Langerhans cell–targeted mRNA delivery:
A strategy for dose-sparing and enhanced antitumor immunity. Journal of Investigative
Dermatology. doi:10.1016/j.jid.2026.03.026'
apa: 'Klein, K., Johnson, L., Rîca, R., Sarcevic, M., Carta, G., Seiser, S., … Sparber,
F. (n.d.). Langerhans cell–targeted mRNA delivery: A strategy for dose-sparing
and enhanced antitumor immunity. Journal of Investigative Dermatology.
Elsevier. https://doi.org/10.1016/j.jid.2026.03.026'
chicago: 'Klein, Klara, Litty Johnson, Ramona Rîca, Mirza Sarcevic, Gabriele Carta,
Saskia Seiser, Adelheid Elbe-Bürger, et al. “Langerhans Cell–Targeted MRNA Delivery:
A Strategy for Dose-Sparing and Enhanced Antitumor Immunity.” Journal of Investigative
Dermatology. Elsevier, n.d. https://doi.org/10.1016/j.jid.2026.03.026.'
ieee: 'K. Klein et al., “Langerhans cell–targeted mRNA delivery: A strategy
for dose-sparing and enhanced antitumor immunity,” Journal of Investigative
Dermatology. Elsevier.'
ista: 'Klein K, Johnson L, Rîca R, Sarcevic M, Carta G, Seiser S, Elbe-Bürger A,
Langer F, Rahhal N, Rademacher C, Wawrzinek R, Quattrone F, Sparber F. Langerhans
cell–targeted mRNA delivery: A strategy for dose-sparing and enhanced antitumor
immunity. Journal of Investigative Dermatology.'
mla: 'Klein, Klara, et al. “Langerhans Cell–Targeted MRNA Delivery: A Strategy for
Dose-Sparing and Enhanced Antitumor Immunity.” Journal of Investigative Dermatology,
Elsevier, doi:10.1016/j.jid.2026.03.026.'
short: K. Klein, L. Johnson, R. Rîca, M. Sarcevic, G. Carta, S. Seiser, A. Elbe-Bürger,
F. Langer, N. Rahhal, C. Rademacher, R. Wawrzinek, F. Quattrone, F. Sparber, Journal
of Investigative Dermatology (n.d.).
date_created: 2026-05-10T22:02:16Z
date_published: 2026-04-07T00:00:00Z
date_updated: 2026-05-11T06:07:32Z
day: '07'
department:
- _id: PreCl
doi: 10.1016/j.jid.2026.03.026
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1101/2025.06.25.661517
month: '04'
oa: 1
oa_version: Preprint
publication: Journal of Investigative Dermatology
publication_identifier:
eissn:
- 1523-1747
issn:
- 0022-202X
publication_status: inpress
publisher: Elsevier
scopus_import: '1'
status: public
title: 'Langerhans cell–targeted mRNA delivery: A strategy for dose-sparing and enhanced
antitumor immunity'
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2026'
...
---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '21860'
abstract:
- lang: eng
text: Glutamate excitotoxicity is a cell death mechanism triggered by accumulation
of glutamate in the extracellular space. The α-ketoglutarate dehydrogenase complex
(αKGDHC), an enzyme of the tricarboxylic acid cycle, represents a branching point
controlling glutamate formation and its consumption as a fuel. Hence, modulation
of the activity of αKGDHC might alter the amount of glutamate available for excitotoxic
effects. To address this hypothesis, hippocampal neurons in primary co-culture
with glial cells were exposed to zero-Mg2 buffer to elicit excitotoxicity through
N-methyl-D-aspartic acid (NMDA) receptor disinhibition. Pretreatment of the cultures
with succinyl phosphonate, to inhibit αKGDHC, enhanced excitotoxity, whereas promotion
of αKGDHC activity by pretreatment with thiamine caused an opposite action. Moreover,
NMDA receptor currents – but not those mediated by α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic
acid (AMPA) receptors – were potentiated in neurons with impaired αKGDHC activity
and diminished in neurons with boosted αKGDHC activity. The sensitization of NMDA
receptors involved mGluR1 activation and was accompanied by enhanced neuronal
discharge activity, elevated basal cytosolic Ca2+ levels, and augmented Ca2+ responses
evoked by glutamate application. These results suggest that mGluR1-mediated potentiation
of NMDA receptors contributes to a mechanism by which inhibition of αKGDHC might
exacerbate glutamate excitotoxicity.
acknowledgement: The technical assistance by Tanja Wagner and Elena Lilliu is gratefully
acknowledged. This research was funded in whole or in part by the Austrian Science
Fund (FWF) (P36145 to H.K., PAT8605623 to M.H. and P33799 to A.V.K.]. Open Access
funding provided by Medical University of Vienna and the Austrian Science Fund (FWF).
Deposited in PMC for immediate release.
article_number: jcs264420
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Vanessa
full_name: Goeschl, Vanessa
last_name: Goeschl
- first_name: Matej
full_name: Hotka, Matej
last_name: Hotka
- first_name: Bernhard
full_name: Hochreiter, Bernhard
id: e6cab3de-17f6-11ed-9210-c1e42e045e9d
last_name: Hochreiter
- first_name: Karlheinz
full_name: Hilber, Karlheinz
last_name: Hilber
- first_name: Stefan
full_name: Boehm, Stefan
last_name: Boehm
- first_name: Andrey V.
full_name: Kozlov, Andrey V.
last_name: Kozlov
- first_name: Helmut
full_name: Kubista, Helmut
last_name: Kubista
citation:
ama: Goeschl V, Hotka M, Hochreiter B, et al. α-ketoglutarate dehydrogenase complex
activity modulates glutamate excitotoxicity via metabotropic regulation of NMDA
receptors in primary cultures. Journal of Cell Science. 2026;139(8). doi:10.1242/jcs.264420
apa: Goeschl, V., Hotka, M., Hochreiter, B., Hilber, K., Boehm, S., Kozlov, A. V.,
& Kubista, H. (2026). α-ketoglutarate dehydrogenase complex activity modulates
glutamate excitotoxicity via metabotropic regulation of NMDA receptors in primary
cultures. Journal of Cell Science. The Company of Biologists. https://doi.org/10.1242/jcs.264420
chicago: Goeschl, Vanessa, Matej Hotka, Bernhard Hochreiter, Karlheinz Hilber, Stefan
Boehm, Andrey V. Kozlov, and Helmut Kubista. “α-Ketoglutarate Dehydrogenase Complex
Activity Modulates Glutamate Excitotoxicity via Metabotropic Regulation of NMDA
Receptors in Primary Cultures.” Journal of Cell Science. The Company of
Biologists, 2026. https://doi.org/10.1242/jcs.264420.
ieee: V. Goeschl et al., “α-ketoglutarate dehydrogenase complex activity
modulates glutamate excitotoxicity via metabotropic regulation of NMDA receptors
in primary cultures,” Journal of Cell Science, vol. 139, no. 8. The Company
of Biologists, 2026.
ista: Goeschl V, Hotka M, Hochreiter B, Hilber K, Boehm S, Kozlov AV, Kubista H.
2026. α-ketoglutarate dehydrogenase complex activity modulates glutamate excitotoxicity
via metabotropic regulation of NMDA receptors in primary cultures. Journal of
Cell Science. 139(8), jcs264420.
mla: Goeschl, Vanessa, et al. “α-Ketoglutarate Dehydrogenase Complex Activity Modulates
Glutamate Excitotoxicity via Metabotropic Regulation of NMDA Receptors in Primary
Cultures.” Journal of Cell Science, vol. 139, no. 8, jcs264420, The Company
of Biologists, 2026, doi:10.1242/jcs.264420.
short: V. Goeschl, M. Hotka, B. Hochreiter, K. Hilber, S. Boehm, A.V. Kozlov, H.
Kubista, Journal of Cell Science 139 (2026).
date_created: 2026-05-11T10:52:27Z
date_published: 2026-04-27T00:00:00Z
date_updated: 2026-05-12T06:40:18Z
day: '27'
ddc:
- '570'
department:
- _id: Bio
doi: 10.1242/jcs.264420
external_id:
pmid:
- '41834724'
file:
- access_level: open_access
checksum: 8db35c97588c2f6ef88c7e8d5924cf8c
content_type: application/pdf
creator: dernst
date_created: 2026-05-12T06:27:54Z
date_updated: 2026-05-12T06:27:54Z
file_id: '21861'
file_name: 2026_JourCellScience_Goeschl.pdf
file_size: 1957057
relation: main_file
success: 1
file_date_updated: 2026-05-12T06:27:54Z
has_accepted_license: '1'
intvolume: ' 139'
issue: '8'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
publication: Journal of Cell Science
publication_identifier:
eissn:
- 1477-9137
issn:
- 0021-9533
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
scopus_import: '1'
status: public
title: α-ketoglutarate dehydrogenase complex activity modulates glutamate excitotoxicity
via metabotropic regulation of NMDA receptors in primary cultures
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 139
year: '2026'
...
---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '21883'
abstract:
- lang: eng
text: Three-dimensional (3D) printing has rapidly developed from a niche hobbyist
activity into a widely accessible and indispensable technology across multiple
scientific disciplines. Within microscopy, optical engineering laboratories and
imaging core facilities, 3D printing enables creating customised solutions for
sample holders, optical components and everyday laboratory tools that traditionally
required specialised machining. By providing rapid prototyping, low-cost production
and reproducibility, 3D printing facilitates innovation and efficiency in facility
operations. This article provides a perspective on the possibilities, challenges,
and practical aspects of implementing 3D printing within microscopy core facilities.
Instead of providing technical review about 3D printing, we focus on service organisation,
user engagement, resource management and community-driven repositories for design
dissemination. Our aim is to share insights with those considering the implementation
of 3D printing as a service for developing add-on components to ease the operation
of different aspects of the machine-park driven services and those who are managing
advanced instrumentation within research groups.
acknowledged_ssus:
- _id: Bio
- _id: M-Shop
acknowledgement: "This work was supported by the Scientific Service Units (SSU) of
Institute of Science and Technology Austria (ISTA) through resources provided by
the Imaging & Optics Facility (IOF) and the MiBa Machine Shop. Specifically; Robert
Hauschild (IOF), sharing designs, insights and pioneering 3D printing activities
at the Imaging and Optics Facility; Bernhard Hochreiter (IOF), for support and testing
of anoxic chamber. We also thank Ana Rita Carvalho Faria and Oliver Biehlmaier (Biozentrum
University of Basel, Imaging Core Facility) for sharing the design of the adopted
power meter.\r\nOpen Access funding provided by Institute of Science and Technology
Austria."
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Mohammad
full_name: Goudarzi, Mohammad
id: 3384113A-F248-11E8-B48F-1D18A9856A87
last_name: Goudarzi
- first_name: Maximilian
full_name: Schuster, Maximilian
id: 37e65def-d415-11eb-ae59-a7b67be103db
last_name: Schuster
- first_name: Arthur
full_name: Milberger, Arthur
last_name: Milberger
- first_name: Manuel
full_name: Gunkel, Manuel
last_name: Gunkel
- first_name: Stefan
full_name: Terjung, Stefan
last_name: Terjung
- first_name: Gabriel
full_name: Krens, Gabriel
id: 2B819732-F248-11E8-B48F-1D18A9856A87
last_name: Krens
orcid: 0000-0003-4761-5996
citation:
ama: Goudarzi M, Schuster M, Milberger A, Gunkel M, Terjung S, Krens G. 3D printing
in core facilities – Low pain, high gain. Journal of Microscopy. 2026.
doi:10.1111/jmi.70106
apa: Goudarzi, M., Schuster, M., Milberger, A., Gunkel, M., Terjung, S., & Krens,
G. (2026). 3D printing in core facilities – Low pain, high gain. Journal of
Microscopy. Wiley. https://doi.org/10.1111/jmi.70106
chicago: Goudarzi, Mohammad, Maximilian Schuster, Arthur Milberger, Manuel Gunkel,
Stefan Terjung, and Gabriel Krens. “3D Printing in Core Facilities – Low Pain,
High Gain.” Journal of Microscopy. Wiley, 2026. https://doi.org/10.1111/jmi.70106.
ieee: M. Goudarzi, M. Schuster, A. Milberger, M. Gunkel, S. Terjung, and G. Krens,
“3D printing in core facilities – Low pain, high gain,” Journal of Microscopy.
Wiley, 2026.
ista: Goudarzi M, Schuster M, Milberger A, Gunkel M, Terjung S, Krens G. 2026. 3D
printing in core facilities – Low pain, high gain. Journal of Microscopy.
mla: Goudarzi, Mohammad, et al. “3D Printing in Core Facilities – Low Pain, High
Gain.” Journal of Microscopy, Wiley, 2026, doi:10.1111/jmi.70106.
short: M. Goudarzi, M. Schuster, A. Milberger, M. Gunkel, S. Terjung, G. Krens,
Journal of Microscopy (2026).
corr_author: '1'
date_created: 2026-05-17T22:02:11Z
date_published: 2026-05-09T00:00:00Z
date_updated: 2026-05-18T08:55:42Z
day: '09'
ddc:
- '600'
department:
- _id: Bio
doi: 10.1111/jmi.70106
external_id:
pmid:
- '42104760'
has_accepted_license: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1111/jmi.70106
month: '05'
oa: 1
oa_version: Published Version
pmid: 1
publication: Journal of Microscopy
publication_identifier:
eissn:
- 1365-2818
issn:
- 0022-2720
publication_status: epub_ahead
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: 3D printing in core facilities – Low pain, high gain
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2026'
...
---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '18449'
abstract:
- lang: eng
text: "Research involving human subjects or identifiable human material and data
must be assessed by an ethics committee. The Karl Landsteiner University of Health
Sciences has established a Commission on Ethics and Scientific Integrity to evaluate
medical research conducted by its faculty and students and at its affiliated hospitals.\r\nAll
projects submitted to the Commission on Ethics and Scientific Integrity between
2018 and 2023 were analyzed regarding their major characteristics, the duration
of the evaluation process, and votes issued.\r\nA total of 520 applications were
electronically submitted during the observation period. Most of the studies were
retrospective data analyses in the field of oncology, psychology and surgery.
Most studies included less than 100 volunteers. Of the applications 50% received
a final vote within 5 months, during which several revision rounds took place.
Overall, about 77% of votes issued during the observation period were positive
and 2% were rejections. In 11% files were closed due to withdrawal. In 11% final
votes were pending at the end of the observation period due to requests for revisions.\r\nOur
results emphasize the importance of institutional ethics committees using the
example of the Commission on Ethics and Scientific Integrity at the Karl Landsteiner
University. Such committees fill a gap in evaluating research not covered by Austrian
legal regulations. Continuous development of standards, operating procedures,
and national and international collaborations are required to assess and minimize
risks to trial subjects and to provide a safe and productive environment for research
in human medicine and related fields."
acknowledgement: Open access funding provided by Karl Landsteiner University.
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Sophie
full_name: Schober, Sophie
id: 80b0a0ef-4b9f-11ec-b119-8d9d94c4a1d8
last_name: Schober
- first_name: Sascha
full_name: Klee, Sascha
last_name: Klee
- first_name: Franz
full_name: Trautinger, Franz
last_name: Trautinger
citation:
ama: 'Schober S, Klee S, Trautinger F. The role of institutional ethics committees
in Austria: Report of the Commission on Ethics and Scientific Integrity of the
Karl Landsteiner University of Health Sciences 2018–2023. Wiener Klinische
Wochenschrift. 2025;137:432-437. doi:10.1007/s00508-024-02462-x'
apa: 'Schober, S., Klee, S., & Trautinger, F. (2025). The role of institutional
ethics committees in Austria: Report of the Commission on Ethics and Scientific
Integrity of the Karl Landsteiner University of Health Sciences 2018–2023. Wiener
Klinische Wochenschrift. Springer Nature. https://doi.org/10.1007/s00508-024-02462-x'
chicago: 'Schober, Sophie, Sascha Klee, and Franz Trautinger. “The Role of Institutional
Ethics Committees in Austria: Report of the Commission on Ethics and Scientific
Integrity of the Karl Landsteiner University of Health Sciences 2018–2023.” Wiener
Klinische Wochenschrift. Springer Nature, 2025. https://doi.org/10.1007/s00508-024-02462-x.'
ieee: 'S. Schober, S. Klee, and F. Trautinger, “The role of institutional ethics
committees in Austria: Report of the Commission on Ethics and Scientific Integrity
of the Karl Landsteiner University of Health Sciences 2018–2023,” Wiener Klinische
Wochenschrift, vol. 137. Springer Nature, pp. 432–437, 2025.'
ista: 'Schober S, Klee S, Trautinger F. 2025. The role of institutional ethics committees
in Austria: Report of the Commission on Ethics and Scientific Integrity of the
Karl Landsteiner University of Health Sciences 2018–2023. Wiener Klinische Wochenschrift.
137, 432–437.'
mla: 'Schober, Sophie, et al. “The Role of Institutional Ethics Committees in Austria:
Report of the Commission on Ethics and Scientific Integrity of the Karl Landsteiner
University of Health Sciences 2018–2023.” Wiener Klinische Wochenschrift,
vol. 137, Springer Nature, 2025, pp. 432–37, doi:10.1007/s00508-024-02462-x.'
short: S. Schober, S. Klee, F. Trautinger, Wiener Klinische Wochenschrift 137 (2025)
432–437.
corr_author: '1'
date_created: 2024-10-20T22:02:07Z
date_published: 2025-07-01T00:00:00Z
date_updated: 2025-12-30T06:55:59Z
day: '01'
ddc:
- '570'
department:
- _id: PreCl
doi: 10.1007/s00508-024-02462-x
external_id:
isi:
- '001329812000001'
file:
- access_level: open_access
checksum: 321be8a584117feaea9f3feaa28caabd
content_type: application/pdf
creator: dernst
date_created: 2025-12-30T06:54:03Z
date_updated: 2025-12-30T06:54:03Z
file_id: '20880'
file_name: 2025_WrKlinischeWochenschrift_Schober.pdf
file_size: 580791
relation: main_file
success: 1
file_date_updated: 2025-12-30T06:54:03Z
has_accepted_license: '1'
intvolume: ' 137'
isi: 1
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
page: 432-437
publication: Wiener Klinische Wochenschrift
publication_identifier:
eissn:
- 1613-7671
issn:
- 0043-5325
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'The role of institutional ethics committees in Austria: Report of the Commission
on Ethics and Scientific Integrity of the Karl Landsteiner University of Health
Sciences 2018–2023'
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 137
year: '2025'
...
---
OA_type: closed access
_id: '20055'
abstract:
- lang: eng
text: Supercrystals represent three-dimensional orderings of colloidal nanocrystals
(NCs), showcasing collective properties in photonics, phononics, and electronics
applications.1,2 Recent studies have shown that such assemblies are directly produced
during nanocrystal reactions.3–6 However, a fundamental understanding of in situ
formed supercrystals that withstand typical NC purification processes remains
underexplored, which is important for further use. Herein, we report the reaction
precursor-mediated formation of stable PbTe supercrystals. Rationalizing the formation
of these assemblies through small-angle x-ray scattering (SAXS) measurements,
we unveil their formation mechanism. Our findings reveal that the supercrystal
formation occurs in the presence of an excess of lead oleates in the crude solution.
It should be noted that the formed supercrystals can be stabilized under specific
conditions determined by the lead oleate cluster concentration, content of trioctylphosphine
telluride (TOP-Te), NC size and the need of an annealing step at mild conditions.
Furthermore, this approach allows for the continuous growth of a secondary phase
within the supercrystal; for example in the case of PbTe supercrystals, a PbS
shell can be grown on each PbTe NC constituent, resulting in core-shell PbTe-PbS
supercrystals. Our work elucidates that reaction precursors play an important
role in in situ SC formation and stabilization, implying the possibility of applying
this knowledge to other NC reactions.
acknowledged_ssus:
- _id: EM-Fac
- _id: NMR
- _id: LifeSc
acknowledgement: ISTA and the Werner Siemens Foundation financially supported this
work. The Scientific Service Units (SSU) of ISTA supported this research through
resources provided by the Electron Microscopy Facility (EMF), NMR Facility and the
Lab Support Facility (LSF).
article_number: '173'
article_processing_charge: No
author:
- first_name: Seungho
full_name: Lee, Seungho
id: BB243B88-D767-11E9-B658-BC13E6697425
last_name: Lee
orcid: 0000-0002-6962-8598
- first_name: Daniel
full_name: Balazs, Daniel
id: 302BADF6-85FC-11EA-9E3B-B9493DDC885E
last_name: Balazs
orcid: 0000-0001-7597-043X
- first_name: Sharona
full_name: Horta, Sharona
id: 03a7e858-01b1-11ec-8b71-99ae6c4a05bc
last_name: Horta
- first_name: Aiswarya
full_name: Rayaroth Puthiyaveettil, Aiswarya
id: 8aceb01b-8972-11ed-ae7b-d5fe53775add
last_name: Rayaroth Puthiyaveettil
- first_name: Maria
full_name: Ibáñez, Maria
id: 43C61214-F248-11E8-B48F-1D18A9856A87
last_name: Ibáñez
orcid: 0000-0001-5013-2843
citation:
ama: 'Lee S, Balazs D, Horta S, Rayaroth Puthiyaveettil A, Ibáñez M. Reaction precursor-mediated
formation of stable supercrystals in colloidal nanocrystal synthesis: PbTe case.
In: Proceedings of the MATSUS Spring 2025 Conference. Fundació de la comunitat
valenciana SCITO; 2025. doi:10.29363/nanoge.matsusspring.2025.173'
apa: 'Lee, S., Balazs, D., Horta, S., Rayaroth Puthiyaveettil, A., & Ibáñez,
M. (2025). Reaction precursor-mediated formation of stable supercrystals in colloidal
nanocrystal synthesis: PbTe case. In Proceedings of the MATSUS Spring 2025
Conference. Sevilla, Spain: Fundació de la comunitat valenciana SCITO. https://doi.org/10.29363/nanoge.matsusspring.2025.173'
chicago: 'Lee, Seungho, Daniel Balazs, Sharona Horta, Aiswarya Rayaroth Puthiyaveettil,
and Maria Ibáñez. “Reaction Precursor-Mediated Formation of Stable Supercrystals
in Colloidal Nanocrystal Synthesis: PbTe Case.” In Proceedings of the MATSUS
Spring 2025 Conference. Fundació de la comunitat valenciana SCITO, 2025. https://doi.org/10.29363/nanoge.matsusspring.2025.173.'
ieee: 'S. Lee, D. Balazs, S. Horta, A. Rayaroth Puthiyaveettil, and M. Ibáñez, “Reaction
precursor-mediated formation of stable supercrystals in colloidal nanocrystal
synthesis: PbTe case,” in Proceedings of the MATSUS Spring 2025 Conference,
Sevilla, Spain, 2025.'
ista: 'Lee S, Balazs D, Horta S, Rayaroth Puthiyaveettil A, Ibáñez M. 2025. Reaction
precursor-mediated formation of stable supercrystals in colloidal nanocrystal
synthesis: PbTe case. Proceedings of the MATSUS Spring 2025 Conference. MATSUS:
Materials for Sustainable Development Conference, 173.'
mla: 'Lee, Seungho, et al. “Reaction Precursor-Mediated Formation of Stable Supercrystals
in Colloidal Nanocrystal Synthesis: PbTe Case.” Proceedings of the MATSUS Spring
2025 Conference, 173, Fundació de la comunitat valenciana SCITO, 2025, doi:10.29363/nanoge.matsusspring.2025.173.'
short: S. Lee, D. Balazs, S. Horta, A. Rayaroth Puthiyaveettil, M. Ibáñez, in:,
Proceedings of the MATSUS Spring 2025 Conference, Fundació de la comunitat valenciana
SCITO, 2025.
conference:
end_date: 2025-03-07
location: Sevilla, Spain
name: 'MATSUS: Materials for Sustainable Development Conference'
start_date: 2025-03-03
corr_author: '1'
date_created: 2025-07-21T08:33:20Z
date_published: 2025-03-15T00:00:00Z
date_updated: 2026-02-19T09:25:57Z
day: '15'
department:
- _id: MaIb
- _id: LifeSc
doi: 10.29363/nanoge.matsusspring.2025.173
language:
- iso: eng
month: '03'
oa_version: None
project:
- _id: 9B8F7476-BA93-11EA-9121-9846C619BF3A
name: 'HighTE: The Werner Siemens Laboratory for the High Throughput Discovery of
Semiconductors for Waste Heat Recovery'
publication: Proceedings of the MATSUS Spring 2025 Conference
publication_status: published
publisher: Fundació de la comunitat valenciana SCITO
quality_controlled: '1'
status: public
title: 'Reaction precursor-mediated formation of stable supercrystals in colloidal
nanocrystal synthesis: PbTe case'
type: conference
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2025'
...
---
OA_place: publisher
OA_type: gold
_id: '20146'
abstract:
- lang: eng
text: "This criteria catalogue and the accompanying assessment questions were developed
by a working group of KEMÖ (Kooperation E-Medien Österreich, the Austrian Academic
Library Consortium). They are intended to support research institutions and organisations
in the evaluation of Open Science Infrastructures. The 20 criteria outlined in
the catalogue provide a structured basis for making informed decisions regarding
the financial support of these infrastructures.\r\n\r\nThe assessment questions
are intended to be completed by Open Science Infrastructures and can be shared
with them accordingly."
article_processing_charge: No
author:
- first_name: Paul
full_name: Gredler, Paul
last_name: Gredler
- first_name: Christian
full_name: Kaier, Christian
last_name: Kaier
- first_name: Patrick
full_name: Danowski, Patrick
id: 2EBD1598-F248-11E8-B48F-1D18A9856A87
last_name: Danowski
orcid: 0000-0002-6026-4409
- first_name: Michael
full_name: Zoyer, Michael
last_name: Zoyer
- first_name: Katharina
full_name: Rieck, Katharina
last_name: Rieck
- first_name: Andreas
full_name: Ferus, Andreas
last_name: Ferus
- first_name: Elisabeth
full_name: Rosenberger, Elisabeth
last_name: Rosenberger
- first_name: Alexander
full_name: Löffler, Alexander
last_name: Löffler
- first_name: Lisa
full_name: Hofer, Lisa
last_name: Hofer
- first_name: Laura
full_name: Still, Laura
last_name: Still
citation:
ama: Gredler P, Kaier C, Danowski P, et al. Catalogue of Criteria for Assessing
the Funding Eligibility of Open Science Infrastructures. Zenodo; 2025. doi:10.5281/zenodo.15269364
apa: Gredler, P., Kaier, C., Danowski, P., Zoyer, M., Rieck, K., Ferus, A., … Still,
L. (2025). Catalogue of criteria for assessing the funding eligibility of Open
Science infrastructures. Zenodo. https://doi.org/10.5281/zenodo.15269364
chicago: Gredler, Paul, Christian Kaier, Patrick Danowski, Michael Zoyer, Katharina
Rieck, Andreas Ferus, Elisabeth Rosenberger, Alexander Löffler, Lisa Hofer, and
Laura Still. Catalogue of Criteria for Assessing the Funding Eligibility of
Open Science Infrastructures. Zenodo, 2025. https://doi.org/10.5281/zenodo.15269364.
ieee: P. Gredler et al., Catalogue of criteria for assessing the funding
eligibility of Open Science infrastructures. Zenodo, 2025.
ista: Gredler P, Kaier C, Danowski P, Zoyer M, Rieck K, Ferus A, Rosenberger E,
Löffler A, Hofer L, Still L. 2025. Catalogue of criteria for assessing the funding
eligibility of Open Science infrastructures, Zenodo,p.
mla: Gredler, Paul, et al. Catalogue of Criteria for Assessing the Funding Eligibility
of Open Science Infrastructures. Zenodo, 2025, doi:10.5281/zenodo.15269364.
short: P. Gredler, C. Kaier, P. Danowski, M. Zoyer, K. Rieck, A. Ferus, E. Rosenberger,
A. Löffler, L. Hofer, L. Still, Catalogue of Criteria for Assessing the Funding
Eligibility of Open Science Infrastructures, Zenodo, 2025.
date_created: 2025-08-07T11:10:14Z
date_published: 2025-08-07T00:00:00Z
date_updated: 2025-08-11T07:20:03Z
day: '07'
ddc:
- '020'
department:
- _id: E-Lib
doi: 10.5281/zenodo.15269364
has_accepted_license: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.5281/zenodo.15269364
month: '08'
oa: 1
oa_version: Published Version
publication_status: published
publisher: Zenodo
status: public
title: Catalogue of criteria for assessing the funding eligibility of Open Science
infrastructures
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: working_paper
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2025'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
PlanS_conform: '1'
_id: '20295'
abstract:
- lang: eng
text: 'Scanning Kelvin probe microscopy (SKPM) is a powerful technique for macroscopic
imaging of the electrostatic potential above a surface. Though most often used
to image work-function variations of conductive surfaces, it can also be used
to probe the surface charge on insulating surfaces. In both cases, relating the
measured potential to the underlying signal is non-trivial. Here, general relationships
are derived between the measured SKPM voltage and the underlying source, revealing
either can be cast as a convolution with an appropriately scaled point spread
function (PSF). For charge that exists on a thin insulating layer above a conductor,
the PSF has the same shape as what would occur from a work-function variation
alone, differing by a simple scaling factor. This relationship is confirmed by:
(1) backing it out from finite-element simulations of work-function and charge
signals, and (2) experimentally comparing the measured PSF from a small work-function
target to that from a small charge spot. This scaling factor is further validated
by comparing SKPM charge measurements with Faraday cup measurements for highly
charged samples from contact-charging experiments. These results highlight a heretofore
unappreciated connection between SKPM voltage and charge signals, offering a rigorous
recipe to extract either from experimental data.'
acknowledged_ssus:
- _id: M-Shop
- _id: NanoFab
- _id: ScienComp
- _id: LifeSc
acknowledgement: This project received funding from the European Research Council
(ERC) under the European Union's Horizon 2020 research and innovation programme
(Grant agreement No. 949120). This research was supported by the Scientific Service
Units of The Institute of Science and Technology Austria (ISTA) through resources
provided by the Miba Machine Shop, Nanofabrication Facility, Scientific Computing
Facility, and Lab Support Facility. The authors wish to thank Dmytro Rak and Juan
Carlos Sobarzo for letting us use their equipment. The authors wish to thank Evgeniia
Volobueva for advice in preparing PFIB samples. The authors wish to thank the contributions
of the whole Waitukaitis group for useful discussions and feedback.
article_number: e00521
article_processing_charge: Yes
article_type: original
arxiv: 1
author:
- first_name: Isaac C
full_name: Lenton, Isaac C
id: a550210f-223c-11ec-8182-e2d45e817efb
last_name: Lenton
orcid: 0000-0002-5010-6984
- first_name: Felix
full_name: Pertl, Felix
id: 6313aec0-15b2-11ec-abd3-ed67d16139af
last_name: Pertl
orcid: 0000-0003-0463-5794
- first_name: Lubuna B
full_name: Shafeek, Lubuna B
id: 3CD37A82-F248-11E8-B48F-1D18A9856A87
last_name: Shafeek
orcid: 0000-0001-7180-6050
- first_name: Scott R
full_name: Waitukaitis, Scott R
id: 3A1FFC16-F248-11E8-B48F-1D18A9856A87
last_name: Waitukaitis
orcid: 0000-0002-2299-3176
citation:
ama: Lenton IC, Pertl F, Shafeek LB, Waitukaitis SR. A duality between surface charge
and work function in scanning Kelvin probe microscopy. Advanced Materials Interfaces.
2025;12(19). doi:10.1002/admi.202500521
apa: Lenton, I. C., Pertl, F., Shafeek, L. B., & Waitukaitis, S. R. (2025).
A duality between surface charge and work function in scanning Kelvin probe microscopy.
Advanced Materials Interfaces. Wiley. https://doi.org/10.1002/admi.202500521
chicago: Lenton, Isaac C, Felix Pertl, Lubuna B Shafeek, and Scott R Waitukaitis.
“A Duality between Surface Charge and Work Function in Scanning Kelvin Probe Microscopy.”
Advanced Materials Interfaces. Wiley, 2025. https://doi.org/10.1002/admi.202500521.
ieee: I. C. Lenton, F. Pertl, L. B. Shafeek, and S. R. Waitukaitis, “A duality between
surface charge and work function in scanning Kelvin probe microscopy,” Advanced
Materials Interfaces, vol. 12, no. 19. Wiley, 2025.
ista: Lenton IC, Pertl F, Shafeek LB, Waitukaitis SR. 2025. A duality between surface
charge and work function in scanning Kelvin probe microscopy. Advanced Materials
Interfaces. 12(19), e00521.
mla: Lenton, Isaac C., et al. “A Duality between Surface Charge and Work Function
in Scanning Kelvin Probe Microscopy.” Advanced Materials Interfaces, vol.
12, no. 19, e00521, Wiley, 2025, doi:10.1002/admi.202500521.
short: I.C. Lenton, F. Pertl, L.B. Shafeek, S.R. Waitukaitis, Advanced Materials
Interfaces 12 (2025).
corr_author: '1'
date_created: 2025-09-07T22:01:33Z
date_published: 2025-10-01T00:00:00Z
date_updated: 2025-12-30T09:31:25Z
day: '01'
ddc:
- '530'
department:
- _id: ScWa
- _id: NanoFab
doi: 10.1002/admi.202500521
ec_funded: 1
external_id:
arxiv:
- '2506.07187'
isi:
- '001560163400001'
file:
- access_level: open_access
checksum: 906fcc7733be8ce8a83600427b82cd5a
content_type: application/pdf
creator: dernst
date_created: 2025-12-30T09:31:11Z
date_updated: 2025-12-30T09:31:11Z
file_id: '20908'
file_name: 2025_AdvMaterialsInterfaces_Lenton.pdf
file_size: 1830117
relation: main_file
success: 1
file_date_updated: 2025-12-30T09:31:11Z
has_accepted_license: '1'
intvolume: ' 12'
isi: 1
issue: '19'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
project:
- _id: 0aa60e99-070f-11eb-9043-a6de6bdc3afa
call_identifier: H2020
grant_number: '949120'
name: 'Tribocharge: a multi-scale approach to an enduring problem in physics'
publication: Advanced Materials Interfaces
publication_identifier:
eissn:
- 2196-7350
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: A duality between surface charge and work function in scanning Kelvin probe
microscopy
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 12
year: '2025'
...
---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '20321'
abstract:
- lang: eng
text: Microsecond-to-millisecond motions are instrumental for many biomolecular
functions, including enzymatic activity and ligand binding. Bloch-McConnell Relaxation
Dispersion (BMRD) Nuclear Magnetic Resonance (NMR) spectroscopy is a key technique
for studying these dynamic processes. While BMRD experiments are routinely used
to probe protein motions in solution, the experiment is more demanding in the
solid state, where dipolar couplings complicate the spin dynamics. It is believed
that high deuteration levels are required and sufficient to obtain accurate and
quantitative data. Here we show that even under fast magic-angle spinning and
high levels of deuteration artifactual “bumps” in 15N R1ρ BMRD profiles are common.
The origin of these artifacts is identified as a second-order three-spin Mixed
Rotational and Rotary Resonance (MIRROR) recoupling condition. These artifacts
are found to be a significant confounding factor for the accurate quantification
of microsecond protein dynamics using BMRD in the solid state. We show that the
application of low-power continuous wave (CW) decoupling simultaneously with the
15N spin-lock leads to the suppression of these conditions and enables quantitative
measurements of microsecond exchange in the solid state. Remarkably, the application
of decoupling allows the measurement of accurate BMRD even in fully protonated
proteins at 100 kHz MAS, thus extending the scope of μs dynamics measurements
in MAS NMR.
acknowledged_ssus:
- _id: NMR
- _id: LifeSc
acknowledgement: The authors thank Alexey Krushelnitsky for useful discussions. C.P.J.
thanks NSF (MCB-2303862) and NIH (R35GM156238 and S10OD012303) for funding. This
research was supported by the Scientific Service Units (SSU) of Institute of Science
and Technology Austria (ISTA) through resources provided by the Nuclear Magnetic
Resonance and the Lab Support Facilities.
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Benjamin
full_name: Tatman, Benjamin
id: 71cda2f3-e604-11ee-a1df-da10587eda3f
last_name: Tatman
- first_name: Vidhyalakshmi
full_name: Sridharan, Vidhyalakshmi
last_name: Sridharan
- first_name: Motilal
full_name: Uttarkabat, Motilal
last_name: Uttarkabat
- first_name: Christopher P.
full_name: Jaroniec, Christopher P.
last_name: Jaroniec
- first_name: Matthias
full_name: Ernst, Matthias
last_name: Ernst
- first_name: Petra
full_name: Rovo, Petra
id: c316e53f-b965-11eb-b128-bb26acc59c00
last_name: Rovo
orcid: 0000-0001-8729-7326
- first_name: Paul
full_name: Schanda, Paul
id: 7B541462-FAF6-11E9-A490-E8DFE5697425
last_name: Schanda
orcid: 0000-0002-9350-7606
citation:
ama: 'Tatman B, Sridharan V, Uttarkabat M, et al. Bumps on the road: The way to
clean relaxation dispersion magic-angle spinning NMR. Journal of the American
Chemical Society. 2025;147(32):29315-29326. doi:10.1021/jacs.5c09057'
apa: 'Tatman, B., Sridharan, V., Uttarkabat, M., Jaroniec, C. P., Ernst, M., Rovo,
P., & Schanda, P. (2025). Bumps on the road: The way to clean relaxation dispersion
magic-angle spinning NMR. Journal of the American Chemical Society. American
Chemical Society. https://doi.org/10.1021/jacs.5c09057'
chicago: 'Tatman, Benjamin, Vidhyalakshmi Sridharan, Motilal Uttarkabat, Christopher
P. Jaroniec, Matthias Ernst, Petra Rovo, and Paul Schanda. “Bumps on the Road:
The Way to Clean Relaxation Dispersion Magic-Angle Spinning NMR.” Journal of
the American Chemical Society. American Chemical Society, 2025. https://doi.org/10.1021/jacs.5c09057.'
ieee: 'B. Tatman et al., “Bumps on the road: The way to clean relaxation
dispersion magic-angle spinning NMR,” Journal of the American Chemical Society,
vol. 147, no. 32. American Chemical Society, pp. 29315–29326, 2025.'
ista: 'Tatman B, Sridharan V, Uttarkabat M, Jaroniec CP, Ernst M, Rovo P, Schanda
P. 2025. Bumps on the road: The way to clean relaxation dispersion magic-angle
spinning NMR. Journal of the American Chemical Society. 147(32), 29315–29326.'
mla: 'Tatman, Benjamin, et al. “Bumps on the Road: The Way to Clean Relaxation Dispersion
Magic-Angle Spinning NMR.” Journal of the American Chemical Society, vol.
147, no. 32, American Chemical Society, 2025, pp. 29315–26, doi:10.1021/jacs.5c09057.'
short: B. Tatman, V. Sridharan, M. Uttarkabat, C.P. Jaroniec, M. Ernst, P. Rovo,
P. Schanda, Journal of the American Chemical Society 147 (2025) 29315–29326.
corr_author: '1'
date_created: 2025-09-10T05:37:19Z
date_published: 2025-08-01T00:00:00Z
date_updated: 2026-01-28T12:36:30Z
day: '01'
ddc:
- '540'
department:
- _id: PaSc
- _id: NMR
doi: 10.1021/jacs.5c09057
external_id:
isi:
- '001542746200001'
pmid:
- '40748291'
file:
- access_level: open_access
checksum: b350d56ddddefea96cebd62c277c0ff5
content_type: application/pdf
creator: dernst
date_created: 2025-09-10T07:53:10Z
date_updated: 2025-09-10T07:53:10Z
file_id: '20337'
file_name: 2025_JACS_Tatman.pdf
file_size: 5235353
relation: main_file
success: 1
file_date_updated: 2025-09-10T07:53:10Z
has_accepted_license: '1'
intvolume: ' 147'
isi: 1
issue: '32'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
page: 29315-29326
pmid: 1
publication: Journal of the American Chemical Society
publication_identifier:
eissn:
- 1520-5126
issn:
- 0002-7863
publication_status: published
publisher: American Chemical Society
quality_controlled: '1'
related_material:
record:
- id: '19696'
relation: used_in_publication
status: public
scopus_import: '1'
status: public
title: 'Bumps on the road: The way to clean relaxation dispersion magic-angle spinning
NMR'
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 147
year: '2025'
...
---
OA_place: publisher
OA_type: hybrid
_id: '19076'
abstract:
- lang: eng
text: For accurate perception and motor control, an animal must distinguish between
sensory experiences elicited by external stimuli and those elicited by its own
actions. The diversity of behaviors and their complex influences on the senses
make this distinction challenging. Here, we uncover an action–cue hub that coordinates
motor commands with visual processing in the brain’s first visual relay. We show
that the ventral lateral geniculate nucleus (vLGN) acts as a corollary discharge
center, integrating visual translational optic flow signals with motor copies
from saccades, locomotion and pupil dynamics. The vLGN relays these signals to
correct action-specific visual distortions and to refine perception, as shown
for the superior colliculus and in a depth-estimation task. Simultaneously, brain-wide
vLGN projections drive corrective actions necessary for accurate visuomotor control.
Our results reveal an extended corollary discharge architecture that refines early
visual transformations and coordinates actions via a distributed hub-and-spoke
network to enable visual perception during action.
acknowledged_ssus:
- _id: ScienComp
- _id: PreCl
- _id: LifeSc
- _id: Bio
acknowledgement: We thank Y. Ben-Simon for generously making viral vectors for retrograde
tracing available, as well as J. Watson and F. Marr for reagents. We also thank
R. Shigemoto, W. Młynarski and members of the Neuroethology group for their comments
on the manuscript and L. Burnett for her schematic drawings. This research was supported
by the Scientific Service Units of ISTA through resources provided by Scientific
Computing, the Preclinical Facility, the Lab Support Facility and the Imaging and
Optics Facility, in particular F. Lange, M. Schunn and T. Asenov. This work was
supported by European Research Council Starting Grant no. 756502 (M.J.) and European
Research Council Consolidator Grant no. 101086580 (M.J.); and EMBO ALTF grant no.
1098-2017 (A.S.) and Human Frontiers Science Program grant no. LT000256/2018-L (A.S.).
Open access funding provided by Institute of Science and Technology (IST Austria).
article_number: '7278'
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Tomas A
full_name: Vega Zuniga, Tomas A
id: 2E7C4E78-F248-11E8-B48F-1D18A9856A87
last_name: Vega Zuniga
- first_name: Anton L
full_name: Sumser, Anton L
id: 3320A096-F248-11E8-B48F-1D18A9856A87
last_name: Sumser
orcid: 0000-0002-4792-1881
- first_name: Olga
full_name: Symonova, Olga
id: 3C0C7BC6-F248-11E8-B48F-1D18A9856A87
last_name: Symonova
orcid: 0000-0003-2012-9947
- first_name: Peter
full_name: Koppensteiner, Peter
id: 3B8B25A8-F248-11E8-B48F-1D18A9856A87
last_name: Koppensteiner
orcid: 0000-0002-3509-1948
- first_name: Florian
full_name: Schmidt, Florian
id: A2EF226A-AF19-11E9-924C-0525E6697425
last_name: Schmidt
- first_name: Maximilian A
full_name: Jösch, Maximilian A
id: 2BD278E6-F248-11E8-B48F-1D18A9856A87
last_name: Jösch
orcid: 0000-0002-3937-1330
citation:
ama: Vega Zuniga TA, Sumser AL, Symonova O, Koppensteiner P, Schmidt F, Jösch MA.
A thalamic hub-and-spoke network enables visual perception during action by coordinating
visuomotor dynamics. Nature Neuroscience. 2025;28. doi:10.1038/s41593-025-01874-w
apa: Vega Zuniga, T. A., Sumser, A. L., Symonova, O., Koppensteiner, P., Schmidt,
F., & Jösch, M. A. (2025). A thalamic hub-and-spoke network enables visual
perception during action by coordinating visuomotor dynamics. Nature Neuroscience.
Springer Nature. https://doi.org/10.1038/s41593-025-01874-w
chicago: Vega Zuniga, Tomas A, Anton L Sumser, Olga Symonova, Peter Koppensteiner,
Florian Schmidt, and Maximilian A Jösch. “A Thalamic Hub-and-Spoke Network Enables
Visual Perception during Action by Coordinating Visuomotor Dynamics.” Nature
Neuroscience. Springer Nature, 2025. https://doi.org/10.1038/s41593-025-01874-w.
ieee: T. A. Vega Zuniga, A. L. Sumser, O. Symonova, P. Koppensteiner, F. Schmidt,
and M. A. Jösch, “A thalamic hub-and-spoke network enables visual perception during
action by coordinating visuomotor dynamics,” Nature Neuroscience, vol.
28. Springer Nature, 2025.
ista: Vega Zuniga TA, Sumser AL, Symonova O, Koppensteiner P, Schmidt F, Jösch MA.
2025. A thalamic hub-and-spoke network enables visual perception during action
by coordinating visuomotor dynamics. Nature Neuroscience. 28, 7278.
mla: Vega Zuniga, Tomas A., et al. “A Thalamic Hub-and-Spoke Network Enables Visual
Perception during Action by Coordinating Visuomotor Dynamics.” Nature Neuroscience,
vol. 28, 7278, Springer Nature, 2025, doi:10.1038/s41593-025-01874-w.
short: T.A. Vega Zuniga, A.L. Sumser, O. Symonova, P. Koppensteiner, F. Schmidt,
M.A. Jösch, Nature Neuroscience 28 (2025).
corr_author: '1'
date_created: 2025-02-23T23:01:58Z
date_published: 2025-03-01T00:00:00Z
date_updated: 2025-09-30T10:40:49Z
day: '01'
department:
- _id: MaJö
- _id: PreCl
doi: 10.1038/s41593-025-01874-w
ec_funded: 1
external_id:
isi:
- '001416866800001'
pmid:
- '39930095'
has_accepted_license: '1'
intvolume: ' 28'
isi: 1
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1038/s41593-025-01874-w
month: '03'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 2634E9D2-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '756502'
name: Circuits of Visual Attention
- _id: bdaf81a8-d553-11ed-ba76-c95961984540
grant_number: '101086580'
name: 'Action Selection in the Midbrain: Neuromodulation of Visuomotor Senses'
- _id: 264FEA02-B435-11E9-9278-68D0E5697425
grant_number: ALTF 1098-2017
name: Connecting sensory with motor processing in the superior colliculus
- _id: 266D407A-B435-11E9-9278-68D0E5697425
grant_number: LT000256
name: Neuronal networks of salience and spatial detection in the murine superior
colliculus
publication: Nature Neuroscience
publication_identifier:
eissn:
- 1546-1726
issn:
- 1097-6256
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- description: News on ISTA Website
relation: press_release
url: https://ista.ac.at/en/news/high-tech-video-optimization-in-our-brain/
record:
- id: '18579'
relation: research_data
status: public
scopus_import: '1'
status: public
title: A thalamic hub-and-spoke network enables visual perception during action by
coordinating visuomotor dynamics
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 28
year: '2025'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '19404'
abstract:
- lang: eng
text: Cell migration is a fundamental process during embryonic development. Most
studies in vivo have focused on the migration of cells using the extracellular
matrix (ECM) as their substrate for migration. In contrast, much less is known
about how cells migrate on other cells, as found in early embryos when the ECM
has not yet formed. Here, we show that lateral mesendoderm (LME) cells in the
early zebrafish gastrula use the ectoderm as their substrate for migration. We
show that the lateral ectoderm is permissive for the animal-pole-directed migration
of LME cells, while the ectoderm at the animal pole halts it. These differences
in permissiveness depend on the lateral ectoderm being more cohesive than the
animal ectoderm, a property controlled by bone morphogenetic protein (BMP) signaling
within the ectoderm. Collectively, these findings identify ectoderm tissue cohesion
as one critical factor in regulating LME migration during zebrafish gastrulation.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
- _id: ScienComp
acknowledgement: 'We are grateful to the colleagues who contributed to this work with
discussions, technical advice, and feedback on the manuscript: Irene Steccari, David
Labrousse Arias and the other members of the Heisenberg lab, Nicole Amberg, Florian
Pauler, Nicoletta Petridou, Elena Scarpa, and Edouard Hannezo. We also thank the
Imaging and Optics Facility, the Life Science Facility, and the Scientific Computing
Unit at ISTA for support. The Next Generation Sequencing Facility at Vienna BioCenter
Core Facilities performed the RNA-seq for animal and lateral ectoderm. D.B.B. was
supported by the NOMIS Foundation as a NOMIS Fellow and by an EMBO Postdoctoral
Fellowship (ALTF 343-2022). S. Tavano was supported by an EMBO Postdoctoral Fellowship
(ALTF 1159-2018).'
article_number: '115387'
article_processing_charge: Yes
article_type: original
author:
- first_name: Ste
full_name: Tavano, Ste
id: 2F162F0C-F248-11E8-B48F-1D18A9856A87
last_name: Tavano
orcid: 0000-0001-9970-7804
- first_name: David
full_name: Brückner, David
id: e1e86031-6537-11eb-953a-f7ab92be508d
last_name: Brückner
orcid: 0000-0001-7205-2975
- first_name: Saren
full_name: Tasciyan, Saren
id: 4323B49C-F248-11E8-B48F-1D18A9856A87
last_name: Tasciyan
orcid: 0000-0003-1671-393X
- first_name: Xin
full_name: Tong, Xin
id: 50F65CDC-AA30-11E9-A72B-8A12E6697425
last_name: Tong
- first_name: Roland
full_name: Kardos, Roland
id: 4039350E-F248-11E8-B48F-1D18A9856A87
last_name: Kardos
- first_name: Alexandra
full_name: Schauer, Alexandra
id: 30A536BA-F248-11E8-B48F-1D18A9856A87
last_name: Schauer
orcid: 0000-0001-7659-9142
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Tavano S, Brückner D, Tasciyan S, et al. BMP-dependent patterning of ectoderm
tissue material properties modulates lateral mesendoderm cell migration during
early zebrafish gastrulation. Cell Reports. 2025;44(3). doi:10.1016/j.celrep.2025.115387
apa: Tavano, S., Brückner, D., Tasciyan, S., Tong, X., Kardos, R., Schauer, A.,
… Heisenberg, C.-P. J. (2025). BMP-dependent patterning of ectoderm tissue material
properties modulates lateral mesendoderm cell migration during early zebrafish
gastrulation. Cell Reports. Elsevier. https://doi.org/10.1016/j.celrep.2025.115387
chicago: Tavano, Ste, David Brückner, Saren Tasciyan, Xin Tong, Roland Kardos, Alexandra
Schauer, Robert Hauschild, and Carl-Philipp J Heisenberg. “BMP-Dependent Patterning
of Ectoderm Tissue Material Properties Modulates Lateral Mesendoderm Cell Migration
during Early Zebrafish Gastrulation.” Cell Reports. Elsevier, 2025. https://doi.org/10.1016/j.celrep.2025.115387.
ieee: S. Tavano et al., “BMP-dependent patterning of ectoderm tissue material
properties modulates lateral mesendoderm cell migration during early zebrafish
gastrulation,” Cell Reports, vol. 44, no. 3. Elsevier, 2025.
ista: Tavano S, Brückner D, Tasciyan S, Tong X, Kardos R, Schauer A, Hauschild R,
Heisenberg C-PJ. 2025. BMP-dependent patterning of ectoderm tissue material properties
modulates lateral mesendoderm cell migration during early zebrafish gastrulation.
Cell Reports. 44(3), 115387.
mla: Tavano, Ste, et al. “BMP-Dependent Patterning of Ectoderm Tissue Material Properties
Modulates Lateral Mesendoderm Cell Migration during Early Zebrafish Gastrulation.”
Cell Reports, vol. 44, no. 3, 115387, Elsevier, 2025, doi:10.1016/j.celrep.2025.115387.
short: S. Tavano, D. Brückner, S. Tasciyan, X. Tong, R. Kardos, A. Schauer, R. Hauschild,
C.-P.J. Heisenberg, Cell Reports 44 (2025).
corr_author: '1'
date_created: 2025-03-16T23:01:24Z
date_published: 2025-03-25T00:00:00Z
date_updated: 2025-10-22T07:00:04Z
day: '25'
ddc:
- '570'
department:
- _id: CaHe
- _id: EdHa
- _id: MiSi
- _id: Bio
doi: 10.1016/j.celrep.2025.115387
external_id:
isi:
- '001443652700001'
pmid:
- '40057955'
file:
- access_level: open_access
checksum: 57e05dd1598c807af0afdb32cec039d3
content_type: application/pdf
creator: dernst
date_created: 2025-03-17T10:26:54Z
date_updated: 2025-03-17T10:26:54Z
file_id: '19413'
file_name: 2025_CellReports_Tavano.pdf
file_size: 9067797
relation: main_file
success: 1
file_date_updated: 2025-03-17T10:26:54Z
has_accepted_license: '1'
intvolume: ' 44'
isi: 1
issue: '3'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-nd/4.0/
month: '03'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 34e2a5b5-11ca-11ed-8bc3-b2265616ef0b
grant_number: ALTF 343-2022
name: A mechano-chemical theory for stem cell fate decisions in organoid development
- _id: 269CD5C4-B435-11E9-9278-68D0E5697425
grant_number: ALTF 1159-2018
name: 'Mechanosensation in cell migration: the role of friction forces in cell polarization
and directed migration'
publication: Cell Reports
publication_identifier:
eissn:
- 2211-1247
issn:
- 2639-1856
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: BMP-dependent patterning of ectoderm tissue material properties modulates lateral
mesendoderm cell migration during early zebrafish gastrulation
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 44
year: '2025'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '19663'
abstract:
- lang: eng
text: The centrosome is a microtubule orchestrator, nucleating and anchoring microtubules
that grow radially and exert forces on cargos. At the same time, mechanical stresses
from the microenvironment and cellular shape changes compress and bend microtubules.
Yet, centrosomes are membraneless organelles, raising the question of how centrosomes
withstand mechanical forces. Here, we discover that centrosomes can deform and
even fracture. We reveal that centrosomes experience deformations during navigational
pathfinding within motile cells. Coherence of the centrosome is maintained by
Dyrk3 and cNAP1, preventing fracturing by forces. While cells can compensate for
the depletion of centriolar-based centrosomes, the fracturing of centrosomes impedes
cellular function by generating coexisting microtubule organizing centers that
compete during path navigation and thereby cause cellular entanglement in the
microenvironment. Our findings show that cells actively maintain the integrity
of the centrosome to withstand mechanical forces. These results suggest that centrosome
stability preservation is fundamental, given that almost all cells in multicellular
organisms experience forces.
acknowledgement: "We thank L. Pelkmans and D. Dormann for providing Dyrk3-EGFP plasmids;
M. Heuzé for providing a RFP-Pericentrin plasmid; T. Balla for providing a PH-Akt-GFP
plasmid; E. Snaar-Jagalska for providing a pLenti-V6.3 Ultra-Chili plasmid; T. Tang
for providing CEP120 a plasmid; D. Trono for providing pMD2.G and psSPAX2 plasmids;
M. Sixt for providing EB3-mCherry and EMTB-mCherry plasmids as well as 3T3 fibroblasts,
Lifeact-GFP Hoxb8 cells, and LX293 cells; M. Duggan for RNA isolation from migrating
DCs; M. Schuster from the Biomedical Sequencing Facility at CeMM; J. Schwarz for
providing Jurkat T cells; M. Götz for initial transcriptome analysis; M. Götz and
F. Merino for discussion and sharing reagents; F. Gärtner for discussions and support;
M. Benjamin Braun for critical reading of the manuscript; and the Core Facility
Bioimaging, the Core Facility Flow Cytometry, and the Animal Core Facility of the
Biomedical Center (BMC) for excellent support.\r\nThis work was supported by Peter
Hans Hofschneider Professorship of the Stiftung Experimentelle Biomedizin (J.R.);
German Research Foundation grant “CRC914, project A12” (J.R); German Research Foundation
grant “SPP2332, project 492014049” (J.R.); LMU Institutional Strategy LMU-Excellent
within the framework of the German Excellence Initiative (J.R.); Medical & Clinician
Scientist Program (MCSP) LMU Munich (J.K.); Deutsche Forschungsgemeinschaft (DFG;
German Research Foundation) under Germany’s Excellence Strategy – EXC2151 – 390873048
(D.B.); Deutsche Forschungsgemeinschaft (DFG; German Research Foundation) Grossgeräteantrag
457838313 and under Germany’s Excellence Strategy – EXC 2151 – 390873048 (E.K.);
Ministry of Innovation, Science and Research of North-Rhine-Westphalia (fellowship
AZ: 421-8.03.03.02-137069) (E.K.); TRA Life and Health (University of Bonn) as part
of the Excellence Strategy of the federal and state governments (E.K.); and CZI
grant DAF2020-225401 and grant (DOI https://doi.org/10.37921/120055ratwvi) from
the Chan Zuckerberg Initiative DAF (R.H.)."
article_number: eadx4047
article_processing_charge: Yes
article_type: original
author:
- first_name: Madeleine T.
full_name: Schmitt, Madeleine T.
last_name: Schmitt
- first_name: Janina
full_name: Kroll, Janina
last_name: Kroll
- first_name: Mauricio J.A.
full_name: Ruiz-Fernandez, Mauricio J.A.
last_name: Ruiz-Fernandez
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Shaunak
full_name: Ghosh, Shaunak
last_name: Ghosh
- first_name: Petra
full_name: Kameritsch, Petra
last_name: Kameritsch
- first_name: Jack
full_name: Merrin, Jack
id: 4515C308-F248-11E8-B48F-1D18A9856A87
last_name: Merrin
orcid: 0000-0001-5145-4609
- first_name: Johanna
full_name: Schmid, Johanna
last_name: Schmid
- first_name: Kasia
full_name: Stefanowski, Kasia
last_name: Stefanowski
- first_name: Andreas W.
full_name: Thomae, Andreas W.
last_name: Thomae
- first_name: Jingyuan
full_name: Cheng, Jingyuan
last_name: Cheng
- first_name: Gamze Naz
full_name: Öztan, Gamze Naz
last_name: Öztan
- first_name: Peter
full_name: Konopka, Peter
last_name: Konopka
- first_name: Germán Camargo
full_name: Ortega, Germán Camargo
last_name: Ortega
- first_name: Thomas
full_name: Penz, Thomas
last_name: Penz
- first_name: Luisa
full_name: Bach, Luisa
last_name: Bach
- first_name: Dirk
full_name: Baumjohann, Dirk
last_name: Baumjohann
- first_name: Christoph
full_name: Bock, Christoph
last_name: Bock
- first_name: Tobias
full_name: Straub, Tobias
last_name: Straub
- first_name: Felix
full_name: Meissner, Felix
last_name: Meissner
- first_name: Eva
full_name: Kiermaier, Eva
id: 3EB04B78-F248-11E8-B48F-1D18A9856A87
last_name: Kiermaier
orcid: 0000-0001-6165-5738
- first_name: Jörg
full_name: Renkawitz, Jörg
id: 3F0587C8-F248-11E8-B48F-1D18A9856A87
last_name: Renkawitz
orcid: 0000-0003-2856-3369
citation:
ama: Schmitt MT, Kroll J, Ruiz-Fernandez MJA, et al. Protecting centrosomes from
fracturing enables efficient cell navigation. Science Advances. 2025;11(17).
doi:10.1126/sciadv.adx4047
apa: Schmitt, M. T., Kroll, J., Ruiz-Fernandez, M. J. A., Hauschild, R., Ghosh,
S., Kameritsch, P., … Renkawitz, J. (2025). Protecting centrosomes from fracturing
enables efficient cell navigation. Science Advances. AAAS. https://doi.org/10.1126/sciadv.adx4047
chicago: Schmitt, Madeleine T., Janina Kroll, Mauricio J.A. Ruiz-Fernandez, Robert
Hauschild, Shaunak Ghosh, Petra Kameritsch, Jack Merrin, et al. “Protecting Centrosomes
from Fracturing Enables Efficient Cell Navigation.” Science Advances. AAAS,
2025. https://doi.org/10.1126/sciadv.adx4047.
ieee: M. T. Schmitt et al., “Protecting centrosomes from fracturing enables
efficient cell navigation,” Science Advances, vol. 11, no. 17. AAAS, 2025.
ista: Schmitt MT, Kroll J, Ruiz-Fernandez MJA, Hauschild R, Ghosh S, Kameritsch
P, Merrin J, Schmid J, Stefanowski K, Thomae AW, Cheng J, Öztan GN, Konopka P,
Ortega GC, Penz T, Bach L, Baumjohann D, Bock C, Straub T, Meissner F, Kiermaier
E, Renkawitz J. 2025. Protecting centrosomes from fracturing enables efficient
cell navigation. Science Advances. 11(17), eadx4047.
mla: Schmitt, Madeleine T., et al. “Protecting Centrosomes from Fracturing Enables
Efficient Cell Navigation.” Science Advances, vol. 11, no. 17, eadx4047,
AAAS, 2025, doi:10.1126/sciadv.adx4047.
short: M.T. Schmitt, J. Kroll, M.J.A. Ruiz-Fernandez, R. Hauschild, S. Ghosh, P.
Kameritsch, J. Merrin, J. Schmid, K. Stefanowski, A.W. Thomae, J. Cheng, G.N.
Öztan, P. Konopka, G.C. Ortega, T. Penz, L. Bach, D. Baumjohann, C. Bock, T. Straub,
F. Meissner, E. Kiermaier, J. Renkawitz, Science Advances 11 (2025).
date_created: 2025-05-11T22:02:38Z
date_published: 2025-04-25T00:00:00Z
date_updated: 2025-09-30T12:26:21Z
day: '25'
ddc:
- '570'
department:
- _id: Bio
- _id: NanoFab
doi: 10.1126/sciadv.adx4047
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title: Protecting centrosomes from fracturing enables efficient cell navigation
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