---
OA_place: repository
OA_type: green
_id: '21963'
abstract:
- lang: eng
text: The cerebral cortex consists of immense numbers of neuronal and glial cell-types
derived from radial glial progenitor (RGP) cells. How RGPs generate appropriate
quantities of distinct cortical cell-types to safeguard a brain of correct size,
is not well understood. However, genetic aberration in human, including mutations
in PTEN, lead to cortical malformation such as macrocephaly, albeit with unknown
etiology. Here we utilized Mosaic Analysis with Double Markers (MADM)-based clonal
analysis and single cell phenotyping to decipher the role of Pten in neurogenic
and gliogenic RGP lineage progression during cortical ontogeny. While neurogenic
RGP lineage progression and projection neuron production was moderately altered
in the absence of Pten, cortical astrocyte production was drastically increased.
Through genetic epistasis experiments we show that the loss of Pten uncouples
astrocyte generation from essential growth factor signaling hubs, funneling into
MAPK. Collectively, our results suggest that Pten regulates RGP lineage progression
with distinct sequential functions in cortical projection neurogenesis and astrocyte
production to ensure the emergence of a correctly-sized cerebral cortex.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
- _id: PreCl
acknowledgement: "We thank Kay-Uwe Wagner (Wayne State University) for generously
sharing Jak1/2–flox mouse lines; A.\r\nSommer (VBCF GmbH, NGS Unit) for technical
support; N. Kim, V. Mick, S. Schnabl, S. Gobeil, and L.\r\nAndersen for technical
assistance; all members of the Hippenmeyer lab for discussion and B. Novitch for\r\ncomments
on earlier versions of the manuscript. This research was supported by the Scientific
Service Units\r\n(SSU) of IST Austria through resources provided by the Imaging
and Optics Facility (IOF), Lab Support-\r\n(LSF) and Preclinical Facilities (PCF).
O.A.M received support from the Austrian Academy of Sciences\r\nÖAW (DOC 186584),
and N.A. from FWF Elise Richter Program (Grant V1041T). This work was also\r\nsupported
by IST Austria institutional funds; FWF SFB F78 (Neuro Stem Modulation) to S.H.,
and the\r\nEuropean Research Council (ERC) under the European Union’s Horizon 2020
research and innovation\r\nprogramme (grant agreement No 725780 LinPro) to S.H."
article_processing_charge: No
author:
- first_name: Osvaldo
full_name: Miranda, Osvaldo
id: 862A3C56-A8BF-11E9-B4FA-D9E3E5697425
last_name: Miranda
orcid: 0000-0001-6618-6889
- first_name: Ximena
full_name: Contreras, Ximena
id: 475990FE-F248-11E8-B48F-1D18A9856A87
last_name: Contreras
- first_name: Florian
full_name: Pauler, Florian
id: 48EA0138-F248-11E8-B48F-1D18A9856A87
last_name: Pauler
orcid: 0000-0002-7462-0048
- first_name: Amarbayasgalan
full_name: Davaatseren, Amarbayasgalan
id: 70ADC922-B424-11E9-99E3-BA18E6697425
last_name: Davaatseren
- first_name: Nicole
full_name: Amberg, Nicole
id: 4CD6AAC6-F248-11E8-B48F-1D18A9856A87
last_name: Amberg
orcid: 0000-0002-3183-8207
- first_name: Carmen
full_name: Streicher, Carmen
id: 36BCB99C-F248-11E8-B48F-1D18A9856A87
last_name: Streicher
- first_name: Ana
full_name: Villalba Requena, Ana
id: 68cb85a0-39f7-11eb-9559-9aaab4f6a247
last_name: Villalba Requena
orcid: 0000-0002-5615-5277
- first_name: Anna-Magdalena
full_name: Heger, Anna-Magdalena
id: 4B76FFD2-F248-11E8-B48F-1D18A9856A87
last_name: Heger
- first_name: Corentine
full_name: Marie, Corentine
last_name: Marie
- first_name: Bassem A.
full_name: Hassan, Bassem A.
last_name: Hassan
- first_name: Thomas
full_name: Rülicke, Thomas
last_name: Rülicke
- first_name: Simon
full_name: Hippenmeyer, Simon
id: 37B36620-F248-11E8-B48F-1D18A9856A87
last_name: Hippenmeyer
orcid: 0000-0003-2279-1061
citation:
ama: Miranda O, Contreras X, Pauler F, et al. Pten orchestrates neurogenic radial
glia lineage progression and tunes neocortical astrocyte production. bioRxiv.
doi:10.64898/2026.05.01.722191
apa: Miranda, O., Contreras, X., Pauler, F., Davaatseren, A., Amberg, N., Streicher,
C., … Hippenmeyer, S. (n.d.). Pten orchestrates neurogenic radial glia lineage
progression and tunes neocortical astrocyte production. bioRxiv. https://doi.org/10.64898/2026.05.01.722191
chicago: Miranda, Osvaldo, Ximena Contreras, Florian Pauler, Amarbayasgalan Davaatseren,
Nicole Amberg, Carmen Streicher, Ana Villalba Requena, et al. “Pten Orchestrates
Neurogenic Radial Glia Lineage Progression and Tunes Neocortical Astrocyte Production.”
BioRxiv, n.d. https://doi.org/10.64898/2026.05.01.722191.
ieee: O. Miranda et al., “Pten orchestrates neurogenic radial glia lineage
progression and tunes neocortical astrocyte production,” bioRxiv. .
ista: Miranda O, Contreras X, Pauler F, Davaatseren A, Amberg N, Streicher C, Villalba
Requena A, Heger A-M, Marie C, Hassan BA, Rülicke T, Hippenmeyer S. Pten orchestrates
neurogenic radial glia lineage progression and tunes neocortical astrocyte production.
bioRxiv, 10.64898/2026.05.01.722191.
mla: Miranda, Osvaldo, et al. “Pten Orchestrates Neurogenic Radial Glia Lineage
Progression and Tunes Neocortical Astrocyte Production.” BioRxiv, doi:10.64898/2026.05.01.722191.
short: O. Miranda, X. Contreras, F. Pauler, A. Davaatseren, N. Amberg, C. Streicher,
A. Villalba Requena, A.-M. Heger, C. Marie, B.A. Hassan, T. Rülicke, S. Hippenmeyer,
BioRxiv (n.d.).
corr_author: '1'
date_created: 2026-06-09T08:08:53Z
date_published: 2026-05-05T00:00:00Z
date_updated: 2026-06-16T08:57:20Z
day: '05'
ddc:
- '570'
department:
- _id: SiHi
- _id: PreCl
- _id: GradSch
doi: 10.64898/2026.05.01.722191
ec_funded: 1
fulldoi: https://doi.org/10.64898/2026.05.01.722191
has_accepted_license: '1'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc/4.0/
main_file_link:
- open_access: '1'
url: https://doi.org/10.64898/2026.05.01.722191
month: '05'
oa: 1
oa_version: Preprint
project:
- _id: 059F6AB4-7A3F-11EA-A408-12923DDC885E
grant_number: F7805
name: Stem Cell Modulation in Neural Development and Regeneration/ P05-Molecular
Mechanisms of Neural Stem Cell Lineage Progression
- _id: 260018B0-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '725780'
name: Principles of Neural Stem Cell Lineage Progression in Cerebral Cortex Development
publication: bioRxiv
publication_status: submitted
status: public
title: Pten orchestrates neurogenic radial glia lineage progression and tunes neocortical
astrocyte production
tmp:
image: /images/cc_by_nc.png
legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode
name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
short: CC BY-NC (4.0)
type: preprint
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2026'
...
---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '21948'
abstract:
- lang: eng
text: The cerebral cortex comprises diverse neuron and glial cell types generated
by radial glial progenitors (RGPs) during development. Although RGPs broadly differentiate
according to temporally and spatially regulated molecular logics, the lineage
hierarchies linking individual progenitors to defined cell (sub)types are not
well understood. Clone-resolved transcriptomics, combining molecular barcoding
and single-cell RNA sequencing, allow high-resolution lineage tracing at the single-clone/cell
level across different species and models. In this mini-review, we synthesize
recent advances in this field, uncovering unexpected lineage relationships in
the developing brain, with a particular focus on the cerebral cortex. We further
highlight new insights into species-specific differences in the developmental
programs generating cell-type diversity, linking changes in clonal architecture
to lineage diversification during cortical evolution.
acknowledgement: We wish to thank all members of the Hippenmeyer laboratory at ISTA
for exciting discussions on the subject of this review. We apologize to colleagues
whose work we could not cite and/or discuss in the frame of the available space.
Work in the Hippenmeyer laboratory on the discussed topic is supported by ISTA institutional
funds, an EMBO LTF (ALTF 994–2023) to F.P., FWF SFB F78 (10.55776/F78) to S.H.,
and FWF Cluster of Excellence COE16 (10.55776/COE16) to S.H.
article_number: '102487'
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Irene
full_name: Varela Martínez, Irene
id: a69b5985-8829-11f0-8fc2-d0af58f64471
last_name: Varela Martínez
- first_name: Fabrizia
full_name: Pipicelli, Fabrizia
id: 649134fd-d012-11ed-8f82-db1e5050f9ba
last_name: Pipicelli
- first_name: Simon
full_name: Hippenmeyer, Simon
id: 37B36620-F248-11E8-B48F-1D18A9856A87
last_name: Hippenmeyer
orcid: 0000-0003-2279-1061
citation:
ama: 'Varela Martínez I, Pipicelli F, Hippenmeyer S. Tracing cell lineages in the
developing brain: Insights from mosaic analysis and clone-resolved transcriptomics.
Current Opinion in Genetics & Development. 2026;99. doi:10.1016/j.gde.2026.102487'
apa: 'Varela Martínez, I., Pipicelli, F., & Hippenmeyer, S. (2026). Tracing
cell lineages in the developing brain: Insights from mosaic analysis and clone-resolved
transcriptomics. Current Opinion in Genetics & Development. Elsevier.
https://doi.org/10.1016/j.gde.2026.102487'
chicago: 'Varela Martínez, Irene, Fabrizia Pipicelli, and Simon Hippenmeyer. “Tracing
Cell Lineages in the Developing Brain: Insights from Mosaic Analysis and Clone-Resolved
Transcriptomics.” Current Opinion in Genetics & Development. Elsevier,
2026. https://doi.org/10.1016/j.gde.2026.102487.'
ieee: 'I. Varela Martínez, F. Pipicelli, and S. Hippenmeyer, “Tracing cell lineages
in the developing brain: Insights from mosaic analysis and clone-resolved transcriptomics,”
Current Opinion in Genetics & Development, vol. 99. Elsevier, 2026.'
ista: 'Varela Martínez I, Pipicelli F, Hippenmeyer S. 2026. Tracing cell lineages
in the developing brain: Insights from mosaic analysis and clone-resolved transcriptomics.
Current Opinion in Genetics & Development. 99, 102487.'
mla: 'Varela Martínez, Irene, et al. “Tracing Cell Lineages in the Developing Brain:
Insights from Mosaic Analysis and Clone-Resolved Transcriptomics.” Current
Opinion in Genetics & Development, vol. 99, 102487, Elsevier, 2026, doi:10.1016/j.gde.2026.102487.'
short: I. Varela Martínez, F. Pipicelli, S. Hippenmeyer, Current Opinion in Genetics
& Development 99 (2026).
corr_author: '1'
date_created: 2026-06-07T22:01:35Z
date_published: 2026-05-29T00:00:00Z
date_updated: 2026-08-12T09:56:19Z
day: '29'
ddc:
- '570'
department:
- _id: SiHi
doi: 10.1016/j.gde.2026.102487
external_id:
pmid:
- '42214837'
fulldoi: https://doi.org/10.1016/j.gde.2026.102487
has_accepted_license: '1'
intvolume: ' 99'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1016/j.gde.2026.102487
month: '05'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 7c084566-9f16-11ee-852c-c88a1dbbf1cf
grant_number: ALTF 994-2023
name: Role of cell lineage in generating cell-type diversity in developing neocortex’
- _id: 059F6AB4-7A3F-11EA-A408-12923DDC885E
grant_number: F7805
name: Stem Cell Modulation in Neural Development and Regeneration/ P05-Molecular
Mechanisms of Neural Stem Cell Lineage Progression
publication: Current Opinion in Genetics & Development
publication_identifier:
eissn:
- 1879-0380
issn:
- 0959-437X
publication_status: epub_ahead
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Tracing cell lineages in the developing brain: Insights from mosaic analysis
and clone-resolved transcriptomics'
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 99
year: '2026'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
PlanS_conform: '1'
_id: '22714'
abstract:
- lang: eng
text: Radial glial progenitors (RGPs) generate all projection neurons (PNs) in the
cerebral cortex through incompletely understood processes. We combined Mosaic
Analysis with Double Markers at embryonic stages (E)12.5 and E13.5 with early
postnatal callosal tracing to dissect RGP lineage progression. We find that multipotent
RGPs generate all extra-telencephalic (ET) and intra-telencephalic (IT) PNs via
parallel sublineages that emerge simultaneously at neurogenesis onset. ET-PN production
progresses exclusively via small, self-consuming lineages; IT-PN lineages feature
RGPs generating large translaminar outputs. The early emergence of IT-PN–fated
RGPs, coinciding with a switch to direct neurogenesis, contributes to the stereotyped
population-level progression of the multipotent lineage. We also identify POU3F
transcription factors as candidate regulators of IT-PN fate via noncanonical mitotic
chromatin binding. The results support a model whereby IT- and ET-PNs arise from
an early bifurcation and parallel specification within the multipotent RGP lineage.
acknowledgement: 'We thank M. caouyette for the plasmid construction for Pou3f1overexpression;
d. Pinto-Benito for valuable assistance with shRnA validation in n2A cells andqPcR
experiments; c. Varela-Martínez for help with the code for graphical analysis; allmembers
from the nieto’s lab for comment on the manuscript, specially to F. Martín for theinsightful
discussions; J. c. Oliveros and J. A. García from the computational service of the
cnBfor help with the analysis of RnAseq dataset; c. O. Sorzano for help with statistical
analysis; andA. Oña and the service of Advance Optical Microscopy of the cnB for
technical advice.Funding: i.V.-M. holds a fellowship funded by MciciU (PRe-2018-083376)
and 2023 eMBOscientific exchange grant 10214. the work was funded by grants to M.n.
(Pid2020-112831GB- i00 and Pid2023-146322nB- i00 by Mcin/Aei/10.13039/501100011033
and by“eRdF A way of making europe”).'
article_processing_charge: Yes
article_type: original
author:
- first_name: Irene
full_name: Varela Martínez, Irene
id: a69b5985-8829-11f0-8fc2-d0af58f64471
last_name: Varela Martínez
- first_name: Ana
full_name: Villalba Requena, Ana
id: 68cb85a0-39f7-11eb-9559-9aaab4f6a247
last_name: Villalba Requena
orcid: 0000-0002-5615-5277
- first_name: Jorge
full_name: García-Marqués, Jorge
last_name: García-Marqués
- first_name: Alfonso
full_name: Aguilera, Alfonso
last_name: Aguilera
- first_name: Diogo S.
full_name: Castro, Diogo S.
last_name: Castro
- first_name: Simon
full_name: Hippenmeyer, Simon
id: 37B36620-F248-11E8-B48F-1D18A9856A87
last_name: Hippenmeyer
orcid: 0000-0003-2279-1061
- first_name: Marta
full_name: Nieto, Marta
last_name: Nieto
citation:
ama: Varela Martínez I, Villalba Requena A, García-Marqués J, et al. Early fate
diversification of radial glial progenitors during corticogenesis. Science
Advances. 2026;12(32):eadw5487. doi:10.1126/sciadv.adw5487
apa: Varela Martínez, I., Villalba Requena, A., García-Marqués, J., Aguilera, A.,
Castro, D. S., Hippenmeyer, S., & Nieto, M. (2026). Early fate diversification
of radial glial progenitors during corticogenesis. Science Advances. AAAS.
https://doi.org/10.1126/sciadv.adw5487
chicago: Varela Martínez, Irene, Ana Villalba Requena, Jorge García-Marqués, Alfonso
Aguilera, Diogo S. Castro, Simon Hippenmeyer, and Marta Nieto. “Early Fate Diversification
of Radial Glial Progenitors during Corticogenesis.” Science Advances. AAAS,
2026. https://doi.org/10.1126/sciadv.adw5487.
ieee: I. Varela Martínez et al., “Early fate diversification of radial glial
progenitors during corticogenesis,” Science Advances, vol. 12, no. 32.
AAAS, p. eadw5487, 2026.
ista: Varela Martínez I, Villalba Requena A, García-Marqués J, Aguilera A, Castro
DS, Hippenmeyer S, Nieto M. 2026. Early fate diversification of radial glial progenitors
during corticogenesis. Science Advances. 12(32), eadw5487.
mla: Varela Martínez, Irene, et al. “Early Fate Diversification of Radial Glial
Progenitors during Corticogenesis.” Science Advances, vol. 12, no. 32,
AAAS, 2026, p. eadw5487, doi:10.1126/sciadv.adw5487.
short: I. Varela Martínez, A. Villalba Requena, J. García-Marqués, A. Aguilera,
D.S. Castro, S. Hippenmeyer, M. Nieto, Science Advances 12 (2026) eadw5487.
das_tickbox: '1'
dataavailabilitystatement: 'All data and code needed to evaluate and reproduce the
results in the paper are present in the paper and/or the Supplementary Materials.
Source data underlying all figures (including the clonal atlas) are provided in
the Supplementary Materials. RnA-seq analysis code has been deposited in Zenodo
(dOi: 10.5281/zenodo.14609057). this study did not generate new materials.'
date_created: 2026-08-16T22:01:43Z
date_published: 2026-08-07T00:00:00Z
date_updated: 2026-08-20T05:45:28Z
day: '07'
ddc:
- '570'
department:
- _id: SiHi
doi: 10.1126/sciadv.adw5487
external_id:
pmid:
- '42555737'
file:
- access_level: open_access
checksum: 487c3703387080e8f3c4675d67763f0e
content_type: application/pdf
creator: dernst
date_created: 2026-08-20T05:39:32Z
date_updated: 2026-08-20T05:39:32Z
file_id: '22738'
file_name: 2026_ScienceAdv_VarelaMartinez.pdf
file_size: 3056744
relation: main_file
success: 1
file_date_updated: 2026-08-20T05:39:32Z
fulldoi: https://doi.org/10.1126/sciadv.adw5487
has_accepted_license: '1'
intvolume: ' 12'
issue: '32'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
page: eadw5487
pmid: 1
publication: Science Advances
publication_identifier:
eissn:
- 2375-2548
publication_status: published
publisher: AAAS
quality_controlled: '1'
researchdata_availability: yes
scopus_import: '1'
status: public
supplementarymaterial: yes
title: Early fate diversification of radial glial progenitors during corticogenesis
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 12
year: '2026'
...
---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '22753'
abstract:
- lang: eng
text: Radial glial progenitors (RGPs) produce all excitatory neurons in the developing
cerebral cortex. Mosaic analysis with double markers (MADM)-based lineage tracing
in vivo has revealed a quantitative framework of RGP lineage progression1. Here
we established MADM technology2,3 in mouse embryonic stem cells to probe RGP lineage
progression in a self-organizing cortical organoid system. We found that RGPs
exhibit a high level of plasticity in proliferative potential in organoids rather
than strict temporally stereotyped lineage progression as observed in vivo. RGPs
in organoids showed increased lineage restriction, diminishing cell-type diversity
in clones of cortical projection neurons, despite uniform single-cell transcriptional
signatures of RGPs and a unitary lineage trajectory. Thus, critical non-cell-autonomous
cues that are absent in self-organizing systems and/or the genuine stem cell niche
are essential for faithful temporal control of RGP lineage progression and the
generation of clonal cortical cell-type diversity.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
- _id: PreCl
acknowledgement: We thank M. L. de Guevara, S. Jayaram and A. Heger for technical
assistance with mESC derivation; M. Goudarzi for assistance with organoid imaging;
M. Leeb and F. Freeman for advice in culturing mESCs and organoids; S. Gobeil and
L. Sweeney for reagents and advice for organoid clearing; A. Heger for mouse colony
management; J. Hauser for technical assistance; the Stanford Brain Organogenesis
Workshop; and all members of the Hippenmeyer laboratory for discussion and/or comments
on the manuscript. This study was supported by the Scientific Service Units (SSU)
of the Institute of Science and Technology, Austria through resources provided by
the Imaging and Optics Facility (IOF), Laboratory Support Facility (LSF) and Preclinical
Facility (PCF). M.S. received funding from the European Commission (IST plus postdoctoral
fellowship). This work was supported by ISTA institutional funds to S.H., FWF SFB
F78 Neuro Stem Modulation to S.H., and by the European Research Council (ERC) under
the European Union’s Horizon 2020 Research And Innovation Program (grant agreement
725780 LinPro) to S.H. Open access funding provided by Institute of Science and
Technology (IST Austria).
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Melissa A
full_name: Stouffer, Melissa A
id: 4C9372C4-F248-11E8-B48F-1D18A9856A87
last_name: Stouffer
- first_name: Osvaldo
full_name: Miranda, Osvaldo
id: 862A3C56-A8BF-11E9-B4FA-D9E3E5697425
last_name: Miranda
orcid: 0000-0001-6618-6889
- first_name: Florian
full_name: Pauler, Florian
id: 48EA0138-F248-11E8-B48F-1D18A9856A87
last_name: Pauler
orcid: 0000-0002-7462-0048
- first_name: Fabrizia
full_name: Pipicelli, Fabrizia
id: 649134fd-d012-11ed-8f82-db1e5050f9ba
last_name: Pipicelli
- first_name: Carmen
full_name: Streicher, Carmen
id: 36BCB99C-F248-11E8-B48F-1D18A9856A87
last_name: Streicher
- first_name: Giselle T
full_name: Cheung, Giselle T
id: 471195F6-F248-11E8-B48F-1D18A9856A87
last_name: Cheung
orcid: 0000-0001-8457-2572
- first_name: Simon
full_name: Hippenmeyer, Simon
id: 37B36620-F248-11E8-B48F-1D18A9856A87
last_name: Hippenmeyer
orcid: 0000-0003-2279-1061
citation:
ama: Stouffer MA, Miranda O, Pauler F, et al. Temporal uncoupling of radial glia
lineage progression in cortical organoids. Nature. 2026. doi:10.1038/s41586-026-10916-7
apa: Stouffer, M. A., Miranda, O., Pauler, F., Pipicelli, F., Streicher, C., Cheung,
G. T., & Hippenmeyer, S. (2026). Temporal uncoupling of radial glia lineage
progression in cortical organoids. Nature. Springer Nature. https://doi.org/10.1038/s41586-026-10916-7
chicago: Stouffer, Melissa A, Osvaldo Miranda, Florian Pauler, Fabrizia Pipicelli,
Carmen Streicher, Giselle T Cheung, and Simon Hippenmeyer. “Temporal Uncoupling
of Radial Glia Lineage Progression in Cortical Organoids.” Nature. Springer
Nature, 2026. https://doi.org/10.1038/s41586-026-10916-7.
ieee: M. A. Stouffer et al., “Temporal uncoupling of radial glia lineage
progression in cortical organoids,” Nature. Springer Nature, 2026.
ista: Stouffer MA, Miranda O, Pauler F, Pipicelli F, Streicher C, Cheung GT, Hippenmeyer
S. 2026. Temporal uncoupling of radial glia lineage progression in cortical organoids.
Nature.
mla: Stouffer, Melissa A., et al. “Temporal Uncoupling of Radial Glia Lineage Progression
in Cortical Organoids.” Nature, Springer Nature, 2026, doi:10.1038/s41586-026-10916-7.
short: M.A. Stouffer, O. Miranda, F. Pauler, F. Pipicelli, C. Streicher, G.T. Cheung,
S. Hippenmeyer, Nature (2026).
corr_author: '1'
das_tickbox: '1'
dataavailabilitystatement: All data generated and analysed in this study are included
in the paper, source data and/or Supplementary Tables 2 and 3. Raw sequencing data
have been deposited with Gene Expression Omnibus (GEO) accession number GSE327470.
Source data are provided with this paper. All scripts used to prepare data and figures
for this manuscript are accessible on GitHub at https://github.com/fpauler/Temporal-Uncoupling-of-Radial-Glia-Lineage-Progression-in-Cortical-Organoid.
date_created: 2026-08-23T22:01:47Z
date_published: 2026-08-12T00:00:00Z
date_updated: 2026-09-09T07:11:02Z
day: '12'
ddc:
- '570'
department:
- _id: SiHi
doi: 10.1038/s41586-026-10916-7
ec_funded: 1
external_id:
pmid:
- '42587153'
file:
- access_level: open_access
checksum: 11383e28fc430b28d2666f73833e8b56
content_type: application/pdf
creator: dernst
date_created: 2026-09-07T13:24:10Z
date_updated: 2026-09-07T13:24:10Z
file_id: '22844'
file_name: 2026_Nature_Stouffer.pdf
file_size: 48513068
relation: main_file
success: 1
file_date_updated: 2026-09-07T13:24:10Z
fulldoi: https://doi.org/10.1038/s41586-026-10916-7
has_accepted_license: '1'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 059F6AB4-7A3F-11EA-A408-12923DDC885E
grant_number: F7805
name: Stem Cell Modulation in Neural Development and Regeneration/ P05-Molecular
Mechanisms of Neural Stem Cell Lineage Progression
- _id: 260018B0-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '725780'
name: Principles of Neural Stem Cell Lineage Progression in Cerebral Cortex Development
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
- 0028-0836
publication_status: epub_ahead
publisher: Springer Nature
quality_controlled: '1'
researchdata_availability: yes
scopus_import: '1'
status: public
supplementarymaterial: yes
title: Temporal uncoupling of radial glia lineage progression in cortical organoids
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2026'
...
---
OA_place: publisher
OA_type: gold
_id: '14647'
abstract:
- lang: eng
text: "In the developing vertebrate central nervous system, neurons and glia typically
arise\r\nsequentially from common progenitors. Here, we report that the transcription
factor Forkhead\r\nBox G1 (Foxg1) regulates gliogenesis in the mouse neocortex
via distinct cell-autonomous roles in progenitors and postmitotic neurons that
regulate different aspects of the gliogenic FGF signalling pathway. We demonstrate
that loss of Foxg1 in cortical progenitors at neurogenic stages causes premature
astrogliogenesis. We identify a novel FOXG1 target, the pro-gliogenic FGF pathway
component Fgfr3, which is suppressed by FOXG1 cell-autonomously to maintain neurogenesis.
Furthermore, FOXG1 can also suppress premature astrogliogenesis triggered by the
augmentation of FGF signalling. We identify a second novel function of FOXG1 in
regulating the expression of gliogenic cues in newborn neocortical upper-layer
neurons. Loss of FOXG1 in postmitotic neurons non-autonomously enhances gliogenesis
in the progenitors via FGF signalling. These results fit well with the model that
newborn neurons secrete cues that trigger progenitors to produce the next wave
of cell types, astrocytes. If FGF signalling is attenuated in Foxg1 null progenitors,
they progress to oligodendrocyte production. Therefore, loss of FOXG1 transitions
the progenitor to a gliogenic state, producing either astrocytes or oligodendrocytes
depending on FGF signalling levels. Our results uncover how FOXG1 integrates extrinsic
signalling via the FGF pathway to regulate the sequential generation of neurons,
astrocytes, and oligodendrocytes in the cerebral cortex. "
acknowledgement: "We thank the animal house staff of the Tata Institute of Fundamental
Research, Mumbai (TIFR), for their excellent support; Gordon Fishell (Harvard Medical
School, USA), and Goichi Miyoshi (Gunma University, Japan) for the Foxg1 floxed
mouse line; Hiroshi Kawasaki (Kanazawa University, Japan) for the plasmids pCAG-FGF8
and pCAG-sFgfr3c; Soo Kyung Lee (University at Buffalo, The State University of
New York, USA) for the Foxg1lox/lox genotyping primers and protocol. We thank Deepak
Modi and Vainav Patel (National Institute for Research in Reproductive and Child
Health, NIRRCH, Mumbai, India) for the use of the NIRRCH FACS Facility, and the
staff of the NIRRCH and TIFR FACS facilities for their assistance. We thank Denis
Jabaudon (University of Geneva, Switzerland) for his critical comments on the manuscript
and members of the Jabaudon lab for helpful discussions. This work was funded by
the Department of Atomic Energy (DAE), Govt. of India (Project Identification no.
RTI4003,\r\nDAE OM no. 1303/2/2019/R&D-II/DAE/2079). "
article_number: '101851'
article_processing_charge: Yes
article_type: original
author:
- first_name: Mahima
full_name: Bose, Mahima
last_name: Bose
- first_name: Varun
full_name: Suresh, Varun
last_name: Suresh
- first_name: Urvi
full_name: Mishra, Urvi
last_name: Mishra
- first_name: Ishita
full_name: Talwar, Ishita
last_name: Talwar
- first_name: Anuradha
full_name: Yadav, Anuradha
last_name: Yadav
- first_name: Shiona
full_name: Biswas, Shiona
last_name: Biswas
- first_name: Simon
full_name: Hippenmeyer, Simon
id: 37B36620-F248-11E8-B48F-1D18A9856A87
last_name: Hippenmeyer
orcid: 0000-0003-2279-1061
- first_name: Shubha
full_name: Tole, Shubha
last_name: Tole
citation:
ama: Bose M, Suresh V, Mishra U, et al. Dual role of FOXG1 in regulating gliogenesis
in the developing neocortex via the FGF signalling pathway. eLife. 2025;13.
doi:10.7554/elife.101851.3
apa: Bose, M., Suresh, V., Mishra, U., Talwar, I., Yadav, A., Biswas, S., … Tole,
S. (2025). Dual role of FOXG1 in regulating gliogenesis in the developing neocortex
via the FGF signalling pathway. ELife. eLife Sciences Publications. https://doi.org/10.7554/elife.101851.3
chicago: Bose, Mahima, Varun Suresh, Urvi Mishra, Ishita Talwar, Anuradha Yadav,
Shiona Biswas, Simon Hippenmeyer, and Shubha Tole. “Dual Role of FOXG1 in Regulating
Gliogenesis in the Developing Neocortex via the FGF Signalling Pathway.” ELife.
eLife Sciences Publications, 2025. https://doi.org/10.7554/elife.101851.3.
ieee: M. Bose et al., “Dual role of FOXG1 in regulating gliogenesis in the
developing neocortex via the FGF signalling pathway,” eLife, vol. 13. eLife
Sciences Publications, 2025.
ista: Bose M, Suresh V, Mishra U, Talwar I, Yadav A, Biswas S, Hippenmeyer S, Tole
S. 2025. Dual role of FOXG1 in regulating gliogenesis in the developing neocortex
via the FGF signalling pathway. eLife. 13, 101851.
mla: Bose, Mahima, et al. “Dual Role of FOXG1 in Regulating Gliogenesis in the Developing
Neocortex via the FGF Signalling Pathway.” ELife, vol. 13, 101851, eLife
Sciences Publications, 2025, doi:10.7554/elife.101851.3.
short: M. Bose, V. Suresh, U. Mishra, I. Talwar, A. Yadav, S. Biswas, S. Hippenmeyer,
S. Tole, ELife 13 (2025).
date_created: 2023-12-06T13:07:01Z
date_published: 2025-03-14T00:00:00Z
date_updated: 2025-05-14T11:41:52Z
day: '14'
ddc:
- '570'
department:
- _id: SiHi
doi: 10.7554/elife.101851.3
external_id:
pmid:
- '40085500'
file:
- access_level: open_access
checksum: 64a6a6f86e24b21fe72c7a7fd6056fed
content_type: application/pdf
creator: dernst
date_created: 2025-04-03T11:19:26Z
date_updated: 2025-04-03T11:19:26Z
file_id: '19467'
file_name: 2025_eLife_Bose.pdf
file_size: 17462771
relation: main_file
success: 1
file_date_updated: 2025-04-03T11:19:26Z
fulldoi: https://doi.org/10.7554/elife.101851.3
has_accepted_license: '1'
intvolume: ' 13'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
pmid: 1
publication: eLife
publication_identifier:
eissn:
- 2050-084X
publication_status: published
publisher: eLife Sciences Publications
quality_controlled: '1'
scopus_import: '1'
status: public
title: Dual role of FOXG1 in regulating gliogenesis in the developing neocortex via
the FGF signalling pathway
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 13
year: '2025'
...
---
OA_type: closed access
_id: '18765'
abstract:
- lang: eng
text: Mosaic Analysis with Double Markers (MADM) represents a mouse genetic approach
coupling differential fluorescent labeling to genetic manipulations in dividing
cells and their lineages. MADM uniquely enables the generation and visualization
of individual control or homozygous mutant cells in a heterozygous genetic environment.
Among its diverse applications, MADM has been used to dissect cell-autonomous
gene functions important for cortical development and neural development in general.
The high cellular resolution offered by MADM also permits the analysis of transcriptomic
changes of individual cells upon genetic manipulations. In this chapter, we describe
an experimental protocol combining the generation and isolation of MADM-labeled
cells with downstream single-cell RNA-sequencing technologies to probe cell-type
specific phenotypes due to genetic mutations at single-cell resolution.
acknowledged_ssus:
- _id: Bio
acknowledgement: 'We thank all Hippenmeyer lab members for support and discussions.
Experimental steps described were optimized with support provided by the Imaging
& Optics Facility (IOF) and Preclinical Facility (PCF) at ISTA, Vienna BioCenter
Core Facilities (VBCF), and Christoph Bock lab at Center for Molecular Medicine
(CeMM). G.C. received funding from European Commission (IST plus postdoctoral fellowship).
This work was supported by ISTA institutional funds: The Austrian Science Fund Special
Research Programmes (FWF SFB F78 Neuro Stem Modulation) to S.H.'
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Giselle T
full_name: Cheung, Giselle T
id: 471195F6-F248-11E8-B48F-1D18A9856A87
last_name: Cheung
orcid: 0000-0001-8457-2572
- first_name: Florian
full_name: Pauler, Florian
id: 48EA0138-F248-11E8-B48F-1D18A9856A87
last_name: Pauler
orcid: 0000-0002-7462-0048
- first_name: Simon
full_name: Hippenmeyer, Simon
id: 37B36620-F248-11E8-B48F-1D18A9856A87
last_name: Hippenmeyer
orcid: 0000-0003-2279-1061
citation:
ama: 'Cheung GT, Pauler F, Hippenmeyer S. Probing Cell-Type Specificity of Mutant
Phenotype at Transcriptomic Level Using Mosaic Analysis with Double Markers (MADM).
In: Garcia-Marques J, Lee T, eds. Lineage Tracing. Vol 2886. MIMB. New
York, NY: Springer Nature; 2025:139-151. doi:10.1007/978-1-0716-4310-5_7'
apa: 'Cheung, G. T., Pauler, F., & Hippenmeyer, S. (2025). Probing Cell-Type
Specificity of Mutant Phenotype at Transcriptomic Level Using Mosaic Analysis
with Double Markers (MADM). In J. Garcia-Marques & T. Lee (Eds.), Lineage
Tracing (Vol. 2886, pp. 139–151). New York, NY: Springer Nature. https://doi.org/10.1007/978-1-0716-4310-5_7'
chicago: 'Cheung, Giselle T, Florian Pauler, and Simon Hippenmeyer. “Probing Cell-Type
Specificity of Mutant Phenotype at Transcriptomic Level Using Mosaic Analysis
with Double Markers (MADM).” In Lineage Tracing, edited by Jorge Garcia-Marques
and Tzumin Lee, 2886:139–51. MIMB. New York, NY: Springer Nature, 2025. https://doi.org/10.1007/978-1-0716-4310-5_7.'
ieee: 'G. T. Cheung, F. Pauler, and S. Hippenmeyer, “Probing Cell-Type Specificity
of Mutant Phenotype at Transcriptomic Level Using Mosaic Analysis with Double
Markers (MADM),” in Lineage Tracing, vol. 2886, J. Garcia-Marques and T.
Lee, Eds. New York, NY: Springer Nature, 2025, pp. 139–151.'
ista: 'Cheung GT, Pauler F, Hippenmeyer S. 2025.Probing Cell-Type Specificity of
Mutant Phenotype at Transcriptomic Level Using Mosaic Analysis with Double Markers
(MADM). In: Lineage Tracing. Methods in Molecular Biology, vol. 2886, 139–151.'
mla: Cheung, Giselle T., et al. “Probing Cell-Type Specificity of Mutant Phenotype
at Transcriptomic Level Using Mosaic Analysis with Double Markers (MADM).” Lineage
Tracing, edited by Jorge Garcia-Marques and Tzumin Lee, vol. 2886, Springer
Nature, 2025, pp. 139–51, doi:10.1007/978-1-0716-4310-5_7.
short: G.T. Cheung, F. Pauler, S. Hippenmeyer, in:, J. Garcia-Marques, T. Lee (Eds.),
Lineage Tracing, Springer Nature, New York, NY, 2025, pp. 139–151.
corr_author: '1'
date_created: 2025-01-07T08:36:47Z
date_published: 2025-01-03T00:00:00Z
date_updated: 2025-04-14T07:43:46Z
day: '03'
department:
- _id: SiHi
doi: 10.1007/978-1-0716-4310-5_7
ec_funded: 1
editor:
- first_name: Jorge
full_name: Garcia-Marques, Jorge
last_name: Garcia-Marques
- first_name: Tzumin
full_name: Lee, Tzumin
last_name: Lee
external_id:
pmid:
- '39745639'
fulldoi: https://doi.org/10.1007/978-1-0716-4310-5_7
intvolume: ' 2886'
language:
- iso: eng
month: '01'
oa_version: None
page: 139-151
place: New York, NY
pmid: 1
project:
- _id: 260C2330-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '754411'
name: ISTplus - Postdoctoral Fellowships
publication: Lineage Tracing
publication_identifier:
eisbn:
- '9781071643105'
eissn:
- 1940-6029
isbn:
- '9781071643099'
issn:
- 1064-3745
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
series_title: MIMB
status: public
title: Probing Cell-Type Specificity of Mutant Phenotype at Transcriptomic Level Using
Mosaic Analysis with Double Markers (MADM)
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 2886
year: '2025'
...
---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '19718'
abstract:
- lang: eng
text: The cerebral cortex is arguably the most complex organ in humans. The cortical
architecture is characterized by a remarkable diversity of neuronal and glial
cell types that make up its neuronal circuits. Following a precise temporally
ordered program, radial glia progenitor (RGP) cells generate all cortical excitatory
projection neurons and glial cell-types. Cortical excitatory projection neurons
are produced either directly or via intermediate progenitors, through indirect
neurogenesis. How the extensive cortical cell-type diversity is generated during
cortex development remains, however, a fundamental open question. How do RGPs
quantitatively and qualitatively generate all the neocortical neurons? How does
direct and indirect neurogenesis contribute to the establishment of neuronal and
lineage heterogeneity? Whether RGPs represent a homogeneous and/or multipotent
progenitor population, or if RGPs consist of heterogeneous groups is currently
also not known. In this review, we will summarize the latest findings that contributed
to a deeper insight into the above key questions.
acknowledgement: We wish to thank all members of the Hippenmeyer laboratory at ISTA
for exciting discussions on the subject of this review. We apologize to colleagues
whose work we could not cite and/or discuss in the frame of the available space.
Work in the Hippenmeyer laboratory on the discussed topic is supported by ISTA institutional
funds, an EMBO LTF (ALTF 994–2023) to F.P, and FWF SFB F78 to S.H.
article_number: '103046'
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Fabrizia
full_name: Pipicelli, Fabrizia
id: 649134fd-d012-11ed-8f82-db1e5050f9ba
last_name: Pipicelli
- first_name: Ana
full_name: Villalba Requena, Ana
id: 68cb85a0-39f7-11eb-9559-9aaab4f6a247
last_name: Villalba Requena
orcid: 0000-0002-5615-5277
- first_name: Simon
full_name: Hippenmeyer, Simon
id: 37B36620-F248-11E8-B48F-1D18A9856A87
last_name: Hippenmeyer
orcid: 0000-0003-2279-1061
citation:
ama: Pipicelli F, Villalba Requena A, Hippenmeyer S. How radial glia progenitor
lineages generate cell-type diversity in the developing cerebral cortex. Current
Opinion in Neurobiology. 2025;93. doi:10.1016/j.conb.2025.103046
apa: Pipicelli, F., Villalba Requena, A., & Hippenmeyer, S. (2025). How radial
glia progenitor lineages generate cell-type diversity in the developing cerebral
cortex. Current Opinion in Neurobiology. Elsevier. https://doi.org/10.1016/j.conb.2025.103046
chicago: Pipicelli, Fabrizia, Ana Villalba Requena, and Simon Hippenmeyer. “How
Radial Glia Progenitor Lineages Generate Cell-Type Diversity in the Developing
Cerebral Cortex.” Current Opinion in Neurobiology. Elsevier, 2025. https://doi.org/10.1016/j.conb.2025.103046.
ieee: F. Pipicelli, A. Villalba Requena, and S. Hippenmeyer, “How radial glia progenitor
lineages generate cell-type diversity in the developing cerebral cortex,” Current
Opinion in Neurobiology, vol. 93. Elsevier, 2025.
ista: Pipicelli F, Villalba Requena A, Hippenmeyer S. 2025. How radial glia progenitor
lineages generate cell-type diversity in the developing cerebral cortex. Current
Opinion in Neurobiology. 93, 103046.
mla: Pipicelli, Fabrizia, et al. “How Radial Glia Progenitor Lineages Generate Cell-Type
Diversity in the Developing Cerebral Cortex.” Current Opinion in Neurobiology,
vol. 93, 103046, Elsevier, 2025, doi:10.1016/j.conb.2025.103046.
short: F. Pipicelli, A. Villalba Requena, S. Hippenmeyer, Current Opinion in Neurobiology
93 (2025).
corr_author: '1'
date_created: 2025-05-20T10:20:09Z
date_published: 2025-08-01T00:00:00Z
date_updated: 2025-12-30T10:54:14Z
day: '01'
ddc:
- '570'
department:
- _id: SiHi
doi: 10.1016/j.conb.2025.103046
external_id:
isi:
- '001496227000001'
pmid:
- '40383049'
file:
- access_level: open_access
checksum: 05bacb4acbe6275d43e873dec9ba1d52
content_type: application/pdf
creator: dernst
date_created: 2025-12-30T08:25:49Z
date_updated: 2025-12-30T08:25:49Z
file_id: '20894'
file_name: 2025_CurrentOpNeurobiology_Pipicelli.pdf
file_size: 1592649
relation: main_file
success: 1
file_date_updated: 2025-12-30T08:25:49Z
fulldoi: https://doi.org/10.1016/j.conb.2025.103046
has_accepted_license: '1'
intvolume: ' 93'
isi: 1
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 059F6AB4-7A3F-11EA-A408-12923DDC885E
grant_number: F7805
name: Stem Cell Modulation in Neural Development and Regeneration/ P05-Molecular
Mechanisms of Neural Stem Cell Lineage Progression
- _id: 7c084566-9f16-11ee-852c-c88a1dbbf1cf
grant_number: ALTF 994-2023
name: Role of cell lineage in generating cell-type diversity in developing neocortex’
publication: Current Opinion in Neurobiology
publication_identifier:
issn:
- 0959-4388
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: How radial glia progenitor lineages generate cell-type diversity in the developing
cerebral cortex
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 93
year: '2025'
...
---
OA_type: closed access
_id: '20079'
abstract:
- lang: eng
text: "Research question: Is LINC01638 involved in regulation of epithelial-to-mesenchymal
transition (EMT) in endometriosis?\r\nDesign: A prospective patient cohort study
was combined with functional experiments in the 12Z endometriosis epithelial cell
line to investigate the role of LINC01638 in endometriosis. Eutopic endometrial
samples were collected by curettage, and ectopic endometrial lesion samples were
collected by laparoscopic surgery from 24 control patients and 41 patients with
endometriosis. The phenotype of 12Z cells was assessed following LINC01638 knockdown
using siRNA, performing proliferation, adhesion, migration and invasion assays,
as well as assessing apoptosis and cell cycle changes with flow cytometry assays.
In order to assess the relationship between LINC01638 and histone deacetylase
class 1 enzyme (HDAC1), LINC01638 knockdown was combined with HDAC inhibition
with the specific HDAC inhibitor romidepsin.\r\nResults: LINC01638 was up-regulated
in the epithelial layer of endometriotic lesions, and LINC01638 knockdown in 12Z
cells led to reduced proliferation, adhesion, migration and invasion. The reduction
in proliferation was associated with increased p21 and p27 expression, and G1
phase arrest. Further analysis of LINC01638 control and knockdown cells revealed
that a number of transcription factors associated with EMT are down-regulated
in knockdown cells, along with the cytoskeleton regulatory gene RHOB, while HDAC1
was up-regulated. Chromatin immunoprecipitation analysis and HDAC1 inhibitory
treatment combined with LINC01638 knockdown indicated that LINC01638 regulates
RHOB expression via HDAC1-mediated promoter deacetylation. RHOB is up-regulated
in the epithelial layer of endometriotic lesions compared with eutopic endometrium,
supporting a role in the disease.\r\nConclusions: LINC01638 is an epigenetic regulator
of the pathogenesis of endometriosis, promoting proliferation and EMT of endometriotic
lesions."
acknowledgement: "The authors wish to thank all the participants and health professionals
involved in this study. In addition, the authors wish to thank technical assistants
Barbara Widmar, Matthias Witzmann-Stern and Isabella Haslinger for their work assisting
with this study; and Simon Hippenmeyer for access to bioinformatic infrastructure
and resources.\r\nOpen access funding was provided by the Medical University of
Vienna."
article_number: '104942'
article_processing_charge: No
article_type: original
author:
- first_name: Iveta
full_name: Yotova, Iveta
last_name: Yotova
- first_name: Katharina
full_name: Proestling, Katharina
last_name: Proestling
- first_name: Florian
full_name: Pauler, Florian
id: 48EA0138-F248-11E8-B48F-1D18A9856A87
last_name: Pauler
orcid: 0000-0002-7462-0048
- first_name: Lisa
full_name: Rainer, Lisa
last_name: Rainer
- first_name: Leonie
full_name: Kaup, Leonie
last_name: Kaup
- first_name: Jana
full_name: Heine, Jana
last_name: Heine
- first_name: Lejla
full_name: Sandrieser, Lejla
last_name: Sandrieser
- first_name: René
full_name: Wenzl, René
last_name: Wenzl
- first_name: Quanah J.
full_name: Hudson, Quanah J.
last_name: Hudson
citation:
ama: Yotova I, Proestling K, Pauler F, et al. LINC01638 promotes epithelial-to-mesenchymal
transition in endometriosis epithelial cells by up-regulating RHOB via HDAC1 suppression.
Reproductive Biomedicine Online. 2025;51(3). doi:10.1016/j.rbmo.2025.104942
apa: Yotova, I., Proestling, K., Pauler, F., Rainer, L., Kaup, L., Heine, J., …
Hudson, Q. J. (2025). LINC01638 promotes epithelial-to-mesenchymal transition
in endometriosis epithelial cells by up-regulating RHOB via HDAC1 suppression.
Reproductive Biomedicine Online. Elsevier. https://doi.org/10.1016/j.rbmo.2025.104942
chicago: Yotova, Iveta, Katharina Proestling, Florian Pauler, Lisa Rainer, Leonie
Kaup, Jana Heine, Lejla Sandrieser, René Wenzl, and Quanah J. Hudson. “LINC01638
Promotes Epithelial-to-Mesenchymal Transition in Endometriosis Epithelial Cells
by up-Regulating RHOB via HDAC1 Suppression.” Reproductive Biomedicine Online.
Elsevier, 2025. https://doi.org/10.1016/j.rbmo.2025.104942.
ieee: I. Yotova et al., “LINC01638 promotes epithelial-to-mesenchymal transition
in endometriosis epithelial cells by up-regulating RHOB via HDAC1 suppression,”
Reproductive Biomedicine Online, vol. 51, no. 3. Elsevier, 2025.
ista: Yotova I, Proestling K, Pauler F, Rainer L, Kaup L, Heine J, Sandrieser L,
Wenzl R, Hudson QJ. 2025. LINC01638 promotes epithelial-to-mesenchymal transition
in endometriosis epithelial cells by up-regulating RHOB via HDAC1 suppression.
Reproductive Biomedicine Online. 51(3), 104942.
mla: Yotova, Iveta, et al. “LINC01638 Promotes Epithelial-to-Mesenchymal Transition
in Endometriosis Epithelial Cells by up-Regulating RHOB via HDAC1 Suppression.”
Reproductive Biomedicine Online, vol. 51, no. 3, 104942, Elsevier, 2025,
doi:10.1016/j.rbmo.2025.104942.
short: I. Yotova, K. Proestling, F. Pauler, L. Rainer, L. Kaup, J. Heine, L. Sandrieser,
R. Wenzl, Q.J. Hudson, Reproductive Biomedicine Online 51 (2025).
date_created: 2025-07-27T22:01:25Z
date_published: 2025-07-17T00:00:00Z
date_updated: 2025-09-30T14:10:46Z
day: '17'
department:
- _id: SiHi
doi: 10.1016/j.rbmo.2025.104942
external_id:
isi:
- '001549819000002'
pmid:
- '40680553'
fulldoi: https://doi.org/10.1016/j.rbmo.2025.104942
intvolume: ' 51'
isi: 1
issue: '3'
language:
- iso: eng
month: '07'
oa_version: None
pmid: 1
publication: Reproductive Biomedicine Online
publication_identifier:
eissn:
- 1472-6491
issn:
- 1472-6483
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: LINC01638 promotes epithelial-to-mesenchymal transition in endometriosis epithelial
cells by up-regulating RHOB via HDAC1 suppression
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 51
year: '2025'
...
---
OA_place: publisher
_id: '20737'
acknowledged_ssus:
- _id: Bio
- _id: PreCl
acknowledgement: "I also want to thank ISTA and the Austrian Science Fund FWF SFB
F78 (F7805) for financially\r\nsupporting my research."
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Raquel
full_name: Casado Polanco, Raquel
id: 15240fc1-dbcd-11ea-9d1d-ac5a786425fd
last_name: Casado Polanco
orcid: 0000-0001-8293-4568
citation:
ama: Casado Polanco R. Role of NOTCH signaling in radial glial progenitor lineage
progression. 2025. doi:10.15479/AT-ISTA-20737
apa: Casado Polanco, R. (2025). Role of NOTCH signaling in radial glial progenitor
lineage progression. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-20737
chicago: Casado Polanco, Raquel. “Role of NOTCH Signaling in Radial Glial Progenitor
Lineage Progression.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-20737.
ieee: R. Casado Polanco, “Role of NOTCH signaling in radial glial progenitor lineage
progression,” Institute of Science and Technology Austria, 2025.
ista: Casado Polanco R. 2025. Role of NOTCH signaling in radial glial progenitor
lineage progression. Institute of Science and Technology Austria.
mla: Casado Polanco, Raquel. Role of NOTCH Signaling in Radial Glial Progenitor
Lineage Progression. Institute of Science and Technology Austria, 2025, doi:10.15479/AT-ISTA-20737.
short: R. Casado Polanco, Role of NOTCH Signaling in Radial Glial Progenitor Lineage
Progression, Institute of Science and Technology Austria, 2025.
corr_author: '1'
date_created: 2025-12-09T09:04:18Z
date_published: 2025-12-09T00:00:00Z
date_updated: 2026-04-14T08:16:58Z
day: '09'
ddc:
- '570'
degree_awarded: PhD
department:
- _id: GradSch
- _id: SiHi
doi: 10.15479/AT-ISTA-20737
file:
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checksum: 71e0fdf4619b0d70d03657ad7348137d
content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
creator: rcasadop
date_created: 2025-12-11T09:28:09Z
date_updated: 2025-12-11T11:18:37Z
file_id: '20793'
file_name: 2025_CasadoPolanco_Raquel_Thesis.docx
file_size: 78207207
relation: source_file
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checksum: 58cf2f25c33567723bc754a019c3e396
content_type: application/pdf
creator: rcasadop
date_created: 2025-12-11T09:28:04Z
date_updated: 2025-12-11T09:28:04Z
embargo: 2026-12-01
embargo_to: open_access
file_id: '20794'
file_name: 2025_CasadoPolanco_Raquel_Thesis.pdf
file_size: 6261874
relation: main_file
file_date_updated: 2025-12-11T11:18:37Z
fulldoi: https://doi.org/10.15479/AT-ISTA-20737
has_accepted_license: '1'
keyword:
- NOTCH
- radial glial progenitor
- lineage progression
- cortical development
language:
- iso: eng
month: '12'
oa_version: Published Version
page: '133'
project:
- _id: 059F6AB4-7A3F-11EA-A408-12923DDC885E
grant_number: F7805
name: Stem Cell Modulation in Neural Development and Regeneration/ P05-Molecular
Mechanisms of Neural Stem Cell Lineage Progression
publication_identifier:
isbn:
- 978-3-99078-072-5
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
status: public
supervisor:
- first_name: Simon
full_name: Hippenmeyer, Simon
id: 37B36620-F248-11E8-B48F-1D18A9856A87
last_name: Hippenmeyer
orcid: 0000-0003-2279-1061
title: Role of NOTCH signaling in radial glial progenitor lineage progression
type: dissertation
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
year: '2025'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '8616'
abstract:
- lang: eng
text: The brain vasculature supplies neurons with glucose and oxygen, but little
is known about how vascular plasticity contributes to brain function. Using longitudinal
in vivo imaging, we report that a substantial proportion of blood vessels in the
adult mouse brain sporadically occlude and regress. Their regression proceeds
through sequential stages of blood-flow occlusion, endothelial cell collapse,
relocation or loss of pericytes, and retraction of glial endfeet. Regressing vessels
are found to be widespread in mouse, monkey and human brains. We further reveal
that blood vessel regression cause a reduction of neuronal activity due to a dysfunction
in mitochondrial metabolism and glutamate production. Our results elucidate the
mechanism of vessel regression and its role in neuronal function in the adult
brain.
acknowledgement: 'The project was initiated in the Jan lab at UCSF. We thank Lily
Jan and Yuh-Nung Jan’s generous support. We thank Liqun Luo’s lab for providing
MADM-7 mice and Rolf A Brekken for VEGF-antibodies. Drs. Yuanquan Song (UPenn),
Zhaozhu Hu (JHU), Ji Hu (ShanghaiTech), Yang Xiang (U. Mass), Hao Wang (Zhejiang
U.) and Ruikang Wang (U. Washington) for critical input, colleagues at Children’s
Research Institute, Departments of Neuroscience, Neurology and Neurotherapeutics,
Pediatrics from UT Southwestern, and colleagues from the Jan lab for discussion.
Dr. Bridget Samuels, Sean Morrison (UT Southwestern), and Nannan Lu (Zhejiang U.)
for critical reading. We acknowledge the assistance of the CIBR Imaging core. We
also thank UT Southwestern Live Cell Imaging Facility, a Shared Resource of the
Harold C. Simmons Cancer Center, supported in part by an NCI Cancer Center Support
Grant, P30 CA142543K. This work is supported by CIBR funds and the American Heart
Association AWRP Summer 2016 Innovative Research Grant (17IRG33410377) to W-P.G.;
National Natural Science Foundation of China (No.81370031) to Z.Z.;National Key
Research and Development Program of China (2016YFE0125400)to F.H.;National Natural
Science Foundations of China (No. 81473202) to Y.L.; National Natural Science Foundation
of China (No.31600839) and Shenzhen Science and Technology Research Program (JCYJ20170818163320865)
to B.P.; National Natural Science Foundation of China (No. 31800864) and Westlake
University start-up funds to J-M. J. NIH R01NS088627 to W.L.J.; NIH: R01 AG020670
and RF1AG054111 to H.Z.; R01 NS088555 to A.M.S., and European Research Council No.725780
to S.H.;W-P.G. was a recipient of Bugher-American Heart Association Dan Adams Thinking
Outside the Box Award.'
article_number: '5840'
article_processing_charge: Yes
article_type: original
author:
- first_name: Xiaofei
full_name: Gao, Xiaofei
last_name: Gao
- first_name: Jun-Liszt
full_name: Li, Jun-Liszt
last_name: Li
- first_name: Xingjun
full_name: Chen, Xingjun
last_name: Chen
- first_name: Bo
full_name: Ci, Bo
last_name: Ci
- first_name: Fei
full_name: Chen, Fei
last_name: Chen
- first_name: Nannan
full_name: Lu, Nannan
last_name: Lu
- first_name: Bo
full_name: Shen, Bo
last_name: Shen
- first_name: Lijun
full_name: Zheng, Lijun
last_name: Zheng
- first_name: Jie-Min
full_name: Jia, Jie-Min
last_name: Jia
- first_name: Yating
full_name: Yi, Yating
last_name: Yi
- first_name: Shiwen
full_name: Zhang, Shiwen
last_name: Zhang
- first_name: Ying-Chao
full_name: Shi, Ying-Chao
last_name: Shi
- first_name: Kaibin
full_name: Shi, Kaibin
last_name: Shi
- first_name: Nicholas E
full_name: Propson, Nicholas E
last_name: Propson
- first_name: Yubin
full_name: Huang, Yubin
last_name: Huang
- first_name: Katherine
full_name: Poinsatte, Katherine
last_name: Poinsatte
- first_name: Zhaohuan
full_name: Zhang, Zhaohuan
last_name: Zhang
- first_name: Yuanlei
full_name: Yue, Yuanlei
last_name: Yue
- first_name: Dale B
full_name: Bosco, Dale B
last_name: Bosco
- first_name: Ying-mei
full_name: Lu, Ying-mei
last_name: Lu
- first_name: Shi-bing
full_name: Yang, Shi-bing
last_name: Yang
- first_name: Ralf H.
full_name: Adams, Ralf H.
last_name: Adams
- first_name: Volkhard
full_name: Lindner, Volkhard
last_name: Lindner
- first_name: Fen
full_name: Huang, Fen
last_name: Huang
- first_name: Long-Jun
full_name: Wu, Long-Jun
last_name: Wu
- first_name: Hui
full_name: Zheng, Hui
last_name: Zheng
- first_name: Feng
full_name: Han, Feng
last_name: Han
- first_name: Simon
full_name: Hippenmeyer, Simon
id: 37B36620-F248-11E8-B48F-1D18A9856A87
last_name: Hippenmeyer
orcid: 0000-0003-2279-1061
- first_name: Ann M.
full_name: Stowe, Ann M.
last_name: Stowe
- first_name: Bo
full_name: Peng, Bo
last_name: Peng
- first_name: Marta
full_name: Margeta, Marta
last_name: Margeta
- first_name: Xiaoqun
full_name: Wang, Xiaoqun
last_name: Wang
- first_name: Qiang
full_name: Liu, Qiang
last_name: Liu
- first_name: Jakob
full_name: Körbelin, Jakob
last_name: Körbelin
- first_name: Martin
full_name: Trepel, Martin
last_name: Trepel
- first_name: Hui
full_name: Lu, Hui
last_name: Lu
- first_name: Bo O.
full_name: Zhou, Bo O.
last_name: Zhou
- first_name: Hu
full_name: Zhao, Hu
last_name: Zhao
- first_name: Wenzhi
full_name: Su, Wenzhi
last_name: Su
- first_name: Robert M.
full_name: Bachoo, Robert M.
last_name: Bachoo
- first_name: Woo-ping
full_name: Ge, Woo-ping
last_name: Ge
citation:
ama: Gao X, Li J-L, Chen X, et al. Reduction of neuronal activity mediated by blood-vessel
regression in the brain. Nature Communications. 2025;16. doi:10.1038/s41467-025-60308-0
apa: Gao, X., Li, J.-L., Chen, X., Ci, B., Chen, F., Lu, N., … Ge, W. (2025). Reduction
of neuronal activity mediated by blood-vessel regression in the brain. Nature
Communications. Springer Nature. https://doi.org/10.1038/s41467-025-60308-0
chicago: Gao, Xiaofei, Jun-Liszt Li, Xingjun Chen, Bo Ci, Fei Chen, Nannan Lu, Bo
Shen, et al. “Reduction of Neuronal Activity Mediated by Blood-Vessel Regression
in the Brain.” Nature Communications. Springer Nature, 2025. https://doi.org/10.1038/s41467-025-60308-0.
ieee: X. Gao et al., “Reduction of neuronal activity mediated by blood-vessel
regression in the brain,” Nature Communications, vol. 16. Springer Nature,
2025.
ista: Gao X, Li J-L, Chen X, Ci B, Chen F, Lu N, Shen B, Zheng L, Jia J-M, Yi Y,
Zhang S, Shi Y-C, Shi K, Propson NE, Huang Y, Poinsatte K, Zhang Z, Yue Y, Bosco
DB, Lu Y, Yang S, Adams RH, Lindner V, Huang F, Wu L-J, Zheng H, Han F, Hippenmeyer
S, Stowe AM, Peng B, Margeta M, Wang X, Liu Q, Körbelin J, Trepel M, Lu H, Zhou
BO, Zhao H, Su W, Bachoo RM, Ge W. 2025. Reduction of neuronal activity mediated
by blood-vessel regression in the brain. Nature Communications. 16, 5840.
mla: Gao, Xiaofei, et al. “Reduction of Neuronal Activity Mediated by Blood-Vessel
Regression in the Brain.” Nature Communications, vol. 16, 5840, Springer
Nature, 2025, doi:10.1038/s41467-025-60308-0.
short: X. Gao, J.-L. Li, X. Chen, B. Ci, F. Chen, N. Lu, B. Shen, L. Zheng, J.-M.
Jia, Y. Yi, S. Zhang, Y.-C. Shi, K. Shi, N.E. Propson, Y. Huang, K. Poinsatte,
Z. Zhang, Y. Yue, D.B. Bosco, Y. Lu, S. Yang, R.H. Adams, V. Lindner, F. Huang,
L.-J. Wu, H. Zheng, F. Han, S. Hippenmeyer, A.M. Stowe, B. Peng, M. Margeta, X.
Wang, Q. Liu, J. Körbelin, M. Trepel, H. Lu, B.O. Zhou, H. Zhao, W. Su, R.M. Bachoo,
W. Ge, Nature Communications 16 (2025).
date_created: 2020-10-06T08:58:59Z
date_published: 2025-07-01T00:00:00Z
date_updated: 2025-09-04T07:08:37Z
day: '01'
ddc:
- '570'
department:
- _id: SiHi
doi: 10.1038/s41467-025-60308-0
ec_funded: 1
external_id:
isi:
- '001523450500035'
file:
- access_level: open_access
checksum: f59748cb67232cfb210035d9aef60836
content_type: application/pdf
creator: dernst
date_created: 2025-07-07T09:52:46Z
date_updated: 2025-07-07T09:52:46Z
file_id: '19971'
file_name: 2025_NatureComm_Gao.pdf
file_size: 17018106
relation: main_file
success: 1
file_date_updated: 2025-07-07T09:52:46Z
fulldoi: https://doi.org/10.1038/s41467-025-60308-0
has_accepted_license: '1'
intvolume: ' 16'
isi: 1
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
project:
- _id: 260018B0-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '725780'
name: Principles of Neural Stem Cell Lineage Progression in Cerebral Cortex Development
publication: Nature Communications
publication_identifier:
eissn:
- 2041-1723
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Reduction of neuronal activity mediated by blood-vessel regression in the brain
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 16
year: '2025'
...
---
OA_place: repository
OA_type: green
_id: '19762'
abstract:
- lang: eng
text: The cerebral cortex must contain the appropriate numbers of neurons in each
layer to acquire its proper functional organization. Accordingly, neurogenesis
requires precise regulation along development. Cortical neurons are made either
directly by Radial Glia Cells (RGCs) that self- consume, or indirectly from RGCs
via Intermediate Progenitor Cells (IPCs) and largely preserving the RGC pool.
According to the standing model of cortical development, Direct Neurogenesis predominates
at early stages of development, and progressively shifts to Indirect Neurogenesis,
which predominates at late stages. However, neurogenesis at early stages should
be compatible with RGC amplification, and neurogenesis at late stages needs to
involve RGC consumption, which seems in conflict with the standing model. Here
we studied the modes of neurogenesis along cortical development using multiple
approaches, including birthdating, live imaging and MADM clone labeling. Contrary
to the established dogma, our data show that Indirect Neurogenesis clearly predominates
at early developmental stages, gradually shifting to Direct Neurogenesis at late
stages. These findings challenge the current model of cortical neurogenesis, and
prompt a re-evaluation of previous and ongoing work about the genetic and molecular
mechanisms regulating this process.
acknowledgement: "We thank A. Iñigo for assistance with imaging, and members of the
Borrell and Herrera labs for\r\ninsightful discussions and critical reading of the
manuscript. Funding to our lab members was\r\nprovided by the Spanish Research Agency
(AEI): FPI contract (BES-2016-077737) to L.dV.A., FPI SO contract (SEV-2017-0723-18-1)
to A.E., JdC-Incorporación contract (IJC2020-044653-I) to V.F., and JAE-Intro fellowship
(JAEICU23EX_0071) to I.C., as well as by La Caixa Foundation: La Caixa-Severo Ochoa
fellowship (E-03-2016-0557140) to S.A., INPhINIT-Retaining fellowship (LCF/BQ/DR21/11880012)
to E.F.O., INPhINIT-Incoming fellowship (LCF/BQ/DI22/11940006) to E.N., and Junior
Leader-Retaining grant to A.C. (LCF/BQ/PR23/11980051). Work was supported by grants
from FWF (SFB F78) to S.H.; AEI (PID2021-125618NB-I00) and European Research Council
(101118729) to V.B., who also acknowledges financial support from AEI through the
“Severo Ochoa” Programme for Centers of Excellence in R&D (CEX2021-001165-S)."
article_processing_charge: No
author:
- first_name: Adrián
full_name: Cárdenas, Adrián
last_name: Cárdenas
- first_name: Irem
full_name: Çelik, Irem
last_name: Çelik
- first_name: Alexandre
full_name: Espinós, Alexandre
last_name: Espinós
- first_name: Carmen
full_name: Streicher, Carmen
id: 36BCB99C-F248-11E8-B48F-1D18A9856A87
last_name: Streicher
- first_name: Lara
full_name: López-González, Lara
last_name: López-González
- first_name: Lucia
full_name: del-Valle-Anton, Lucia
last_name: del-Valle-Anton
- first_name: Virginia
full_name: Fernández, Virginia
last_name: Fernández
- first_name: Salma
full_name: Amin, Salma
last_name: Amin
- first_name: Enrico
full_name: Negri, Enrico
last_name: Negri
- first_name: Eduardo Fernández
full_name: Ortuño, Eduardo Fernández
last_name: Ortuño
- first_name: Simon
full_name: Hippenmeyer, Simon
id: 37B36620-F248-11E8-B48F-1D18A9856A87
last_name: Hippenmeyer
orcid: 0000-0003-2279-1061
- first_name: Víctor
full_name: Borrell, Víctor
last_name: Borrell
biorxivid: 1
citation:
ama: Cárdenas A, Çelik I, Espinós A, et al. Early indirect neurogenesis transitions
to late direct neurogenesis in mouse cerebral cortex development. bioRxiv.
doi:10.1101/2025.05.22.655488
apa: Cárdenas, A., Çelik, I., Espinós, A., Streicher, C., López-González, L., del-Valle-Anton,
L., … Borrell, V. (n.d.). Early indirect neurogenesis transitions to late direct
neurogenesis in mouse cerebral cortex development. bioRxiv. https://doi.org/10.1101/2025.05.22.655488
chicago: Cárdenas, Adrián, Irem Çelik, Alexandre Espinós, Carmen Streicher, Lara
López-González, Lucia del-Valle-Anton, Virginia Fernández, et al. “Early Indirect
Neurogenesis Transitions to Late Direct Neurogenesis in Mouse Cerebral Cortex
Development.” BioRxiv, n.d. https://doi.org/10.1101/2025.05.22.655488.
ieee: A. Cárdenas et al., “Early indirect neurogenesis transitions to late
direct neurogenesis in mouse cerebral cortex development,” bioRxiv. .
ista: Cárdenas A, Çelik I, Espinós A, Streicher C, López-González L, del-Valle-Anton
L, Fernández V, Amin S, Negri E, Ortuño EF, Hippenmeyer S, Borrell V. Early indirect
neurogenesis transitions to late direct neurogenesis in mouse cerebral cortex
development. bioRxiv, 10.1101/2025.05.22.655488.
mla: Cárdenas, Adrián, et al. “Early Indirect Neurogenesis Transitions to Late Direct
Neurogenesis in Mouse Cerebral Cortex Development.” BioRxiv, doi:10.1101/2025.05.22.655488.
short: A. Cárdenas, I. Çelik, A. Espinós, C. Streicher, L. López-González, L. del-Valle-Anton,
V. Fernández, S. Amin, E. Negri, E.F. Ortuño, S. Hippenmeyer, V. Borrell, BioRxiv
(n.d.).
date_created: 2025-05-29T10:45:55Z
date_published: 2025-05-23T00:00:00Z
date_updated: 2026-08-13T07:18:38Z
day: '23'
department:
- _id: SiHi
doi: 10.1101/2025.05.22.655488
external_id:
biorxivid:
- 10.1101/2025.05.22.655488
fulldoi: https://doi.org/10.1101/2025.05.22.655488
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1101/2025.05.22.655488
month: '05'
oa: 1
oa_version: Preprint
project:
- _id: 059F6AB4-7A3F-11EA-A408-12923DDC885E
grant_number: F7805
name: Stem Cell Modulation in Neural Development and Regeneration/ P05-Molecular
Mechanisms of Neural Stem Cell Lineage Progression
publication: bioRxiv
publication_status: submitted
status: public
title: Early indirect neurogenesis transitions to late direct neurogenesis in mouse
cerebral cortex development
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: preprint
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2025'
...
---
OA_place: repository
OA_type: green
_id: '19717'
abstract:
- lang: eng
text: Radial glial progenitors (RGPs) generate all projection neurons (PNs) in the
cerebral cortex through incompletely understood processes. Herein, we combine
Mosaic Analysis with Double Markers (MADM)-based clonal analysis at embryonic
days 12.5 and 13.5 with early postnatal callosal tracing to reveal a lineage progression
that challenges the inside-outside model of cortical development and the conventional
view of an invariable sequence of asymmetric neurogenic divisions. Our data demonstrate
that early multipotent RGPs generate all extra-telencephalic (ET) and intra-telencephalic
(IT) PNs across all layers through parallel sublineages and the random specification,
during the earliest neurogenic divisions, of fate-restricted daughter RGPs. While
the neuronal production of the parental multipotent RGPs consists of small ET-PN
or IT-PN outputs, fate-restricted RGPs produce larger translaminar outputs spanning
deep and upper layers of only IT-PNs, the predominant mammalian PN subtype. We
further show that the emergence of IT-PN fate-restricted RGPs also leads to quantitatively
and temporally stereotyped neurogenesis population-wise.
acknowledgement: "We thank M. Caouyette for the plasmid construction for Pou3f1 overexpression;
C. Varela747 Martínez for help with the code for graphical analysis; all members
from the Nieto’s lab for\r\ncomment on the manuscript, specially to F. Martín for
the insightful discussions;J.C. Oliveros\r\nand J.A. García from the computational
service of the CNB for help with the analysis of\r\nRNAseq dataset, C.O. Sorzano
for the help with statistical analysis, and the service of\r\nAdvance Optical Microscopy
of the CNB for their technical advice.\r\nI.V.M holds a fellowship funded by MCICIU
(PRE-2018-083376), the work was funded by\r\nPID2020-112831GB-I00 funded by MCIN/AEI
/10.13039/501100011033.\r\n"
article_processing_charge: No
author:
- first_name: I
full_name: Varela-Martínez, I
last_name: Varela-Martínez
- first_name: Ana
full_name: Villalba Requena, Ana
id: 68cb85a0-39f7-11eb-9559-9aaab4f6a247
last_name: Villalba Requena
orcid: 0000-0002-5615-5277
- first_name: J.
full_name: Garcia-Marqués, J.
last_name: Garcia-Marqués
- first_name: Simon
full_name: Hippenmeyer, Simon
id: 37B36620-F248-11E8-B48F-1D18A9856A87
last_name: Hippenmeyer
orcid: 0000-0003-2279-1061
- first_name: M.
full_name: Nieto, M.
last_name: Nieto
citation:
ama: Varela-Martínez I, Villalba Requena A, Garcia-Marqués J, Hippenmeyer S, Nieto
M. Early emergence of projection-subtype fate-restricted radial glial progenitors
orchestrates neocortical neurogenesis. bioRxiv. doi:10.1101/2025.05.07.652665
apa: Varela-Martínez, I., Villalba Requena, A., Garcia-Marqués, J., Hippenmeyer,
S., & Nieto, M. (n.d.). Early emergence of projection-subtype fate-restricted
radial glial progenitors orchestrates neocortical neurogenesis. bioRxiv.
https://doi.org/10.1101/2025.05.07.652665
chicago: Varela-Martínez, I, Ana Villalba Requena, J. Garcia-Marqués, Simon Hippenmeyer,
and M. Nieto. “Early Emergence of Projection-Subtype Fate-Restricted Radial Glial
Progenitors Orchestrates Neocortical Neurogenesis.” BioRxiv, n.d. https://doi.org/10.1101/2025.05.07.652665.
ieee: I. Varela-Martínez, A. Villalba Requena, J. Garcia-Marqués, S. Hippenmeyer,
and M. Nieto, “Early emergence of projection-subtype fate-restricted radial glial
progenitors orchestrates neocortical neurogenesis,” bioRxiv. .
ista: Varela-Martínez I, Villalba Requena A, Garcia-Marqués J, Hippenmeyer S, Nieto
M. Early emergence of projection-subtype fate-restricted radial glial progenitors
orchestrates neocortical neurogenesis. bioRxiv, 10.1101/2025.05.07.652665.
mla: Varela-Martínez, I., et al. “Early Emergence of Projection-Subtype Fate-Restricted
Radial Glial Progenitors Orchestrates Neocortical Neurogenesis.” BioRxiv,
doi:10.1101/2025.05.07.652665.
short: I. Varela-Martínez, A. Villalba Requena, J. Garcia-Marqués, S. Hippenmeyer,
M. Nieto, BioRxiv (n.d.).
date_created: 2025-05-20T10:19:29Z
date_published: 2025-05-07T00:00:00Z
date_updated: 2026-08-13T07:16:48Z
day: '07'
department:
- _id: SiHi
doi: 10.1101/2025.05.07.652665
fulldoi: https://doi.org/10.1101/2025.05.07.652665
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1101/2025.05.07.652665
month: '05'
oa: 1
oa_version: Preprint
publication: bioRxiv
publication_status: submitted
status: public
title: Early emergence of projection-subtype fate-restricted radial glial progenitors
orchestrates neocortical neurogenesis
type: preprint
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2025'
...
---
OA_place: publisher
_id: '20212'
acknowledged_ssus:
- _id: Bio
- _id: PreCl
acknowledgement: "I would also like to\r\nthank the Austrian Academy of Sciences for
awarding me a 2-year DOC fellowship\r\n(DOC26253)."
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Osvaldo
full_name: Miranda, Osvaldo
id: 862A3C56-A8BF-11E9-B4FA-D9E3E5697425
last_name: Miranda
orcid: 0000-0001-6618-6889
citation:
ama: Miranda O. Unraveling the role of Pten in cortical stem cell lineage progression
using MADM. 2025. doi:10.15479/AT-ISTA-20212
apa: Miranda, O. (2025). Unraveling the role of Pten in cortical stem cell lineage
progression using MADM. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-20212
chicago: Miranda, Osvaldo. “Unraveling the Role of Pten in Cortical Stem Cell Lineage
Progression Using MADM.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-20212.
ieee: O. Miranda, “Unraveling the role of Pten in cortical stem cell lineage progression
using MADM,” Institute of Science and Technology Austria, 2025.
ista: Miranda O. 2025. Unraveling the role of Pten in cortical stem cell lineage
progression using MADM. Institute of Science and Technology Austria.
mla: Miranda, Osvaldo. Unraveling the Role of Pten in Cortical Stem Cell Lineage
Progression Using MADM. Institute of Science and Technology Austria, 2025,
doi:10.15479/AT-ISTA-20212.
short: O. Miranda, Unraveling the Role of Pten in Cortical Stem Cell Lineage Progression
Using MADM, Institute of Science and Technology Austria, 2025.
corr_author: '1'
date_created: 2025-08-22T14:07:00Z
date_published: 2025-08-22T00:00:00Z
date_updated: 2026-08-26T22:30:03Z
day: '22'
ddc:
- '570'
degree_awarded: PhD
department:
- _id: GradSch
- _id: SiHi
doi: 10.15479/AT-ISTA-20212
file:
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checksum: 3331f76bbef74ff4908e2d2c9262045c
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date_created: 2025-08-26T09:03:50Z
date_updated: 2026-08-26T22:30:02Z
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file_name: 2025_MirandaRomero_OsvaldoAntonio_Thesis.docx
file_size: 32887334
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checksum: 02509d50cff8e35c5bcbf71e8d658176
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date_created: 2025-08-26T09:05:55Z
date_updated: 2026-08-26T22:30:02Z
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file_id: '20231'
file_name: 2025_MirandaRomero_OsvaldoAntonio_Thesis.pdf
file_size: 28636240
relation: main_file
file_date_updated: 2026-08-26T22:30:02Z
fulldoi: https://doi.org/10.15479/AT-ISTA-20212
has_accepted_license: '1'
keyword:
- Pten
- mtor
- cortical development
- MADM
- Mapk
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
page: '119'
project:
- _id: 34c9fbcb-11ca-11ed-8bc3-98fa5658610d
grant_number: '26253'
name: Molecular Mechanisms Regulating Cortical Neural Stem Cell Lineage Progression
and Astrocyte Development
publication_identifier:
isbn:
- 978-3-99078-063-3
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '17425'
relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Simon
full_name: Hippenmeyer, Simon
id: 37B36620-F248-11E8-B48F-1D18A9856A87
last_name: Hippenmeyer
orcid: 0000-0003-2279-1061
title: Unraveling the role of Pten in cortical stem cell lineage progression using
MADM
type: dissertation
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
year: '2025'
...
---
_id: '12875'
abstract:
- lang: eng
text: The superior colliculus (SC) in the mammalian midbrain is essential for multisensory
integration and is composed of a rich diversity of excitatory and inhibitory neurons
and glia. However, the developmental principles directing the generation of SC
cell-type diversity are not understood. Here, we pursued systematic cell lineage
tracing in silico and in vivo, preserving full spatial information, using genetic
mosaic analysis with double markers (MADM)-based clonal analysis with single-cell
sequencing (MADM-CloneSeq). The analysis of clonally related cell lineages revealed
that radial glial progenitors (RGPs) in SC are exceptionally multipotent. Individual
resident RGPs have the capacity to produce all excitatory and inhibitory SC neuron
types, even at the stage of terminal division. While individual clonal units show
no pre-defined cellular composition, the establishment of appropriate relative
proportions of distinct neuronal types occurs in a PTEN-dependent manner. Collectively,
our findings provide an inaugural framework at the single-RGP/-cell level of the
mammalian SC ontogeny.
acknowledged_ssus:
- _id: Bio
- _id: M-Shop
- _id: LifeSc
- _id: PreCl
acknowledgement: "We thank Liqun Luo for his continued support, for providing essential
resources for generating Fzd10-CreER mice which were generated in his laboratory,
and for comments on the manuscript; W. Zhong for providing Nestin-Cre transgenic
mouse line for this study; A. Heger for mouse colony management; R. Beattie and
T. Asenov for designing and producing components of acute slice recovery chamber
for MADM-CloneSeq experiments; and K. Leopold, J. Rodarte and N. Amberg for initial
experiments, technical support and/or assistance. This study was supported by the
Scientific Service Units (SSU) of IST Austria through resources provided by the
Imaging & Optics Facility (IOF), Laboratory Support Facility (LSF), Miba Machine
Shop, and Pre-clinical Facility (PCF). G.C. received funding from European Commission
(IST plus postdoctoral fellowship). This work was supported by ISTA institutional\r\nfunds;
the Austrian Science Fund Special Research Programmes (FWF SFB F78 Neuro Stem Modulation)
to S.H. "
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Giselle T
full_name: Cheung, Giselle T
id: 471195F6-F248-11E8-B48F-1D18A9856A87
last_name: Cheung
orcid: 0000-0001-8457-2572
- first_name: Florian
full_name: Pauler, Florian
id: 48EA0138-F248-11E8-B48F-1D18A9856A87
last_name: Pauler
orcid: 0000-0002-7462-0048
- first_name: Peter
full_name: Koppensteiner, Peter
id: 3B8B25A8-F248-11E8-B48F-1D18A9856A87
last_name: Koppensteiner
orcid: 0000-0002-3509-1948
- first_name: Thomas
full_name: Krausgruber, Thomas
last_name: Krausgruber
- first_name: Carmen
full_name: Streicher, Carmen
id: 36BCB99C-F248-11E8-B48F-1D18A9856A87
last_name: Streicher
- first_name: Martin
full_name: Schrammel, Martin
id: f13e7cae-e8bd-11ed-841a-96dedf69f46d
last_name: Schrammel
- first_name: Natalie Y
full_name: Özgen, Natalie Y
id: e68ece33-f6e0-11ea-865d-ae1031dcc090
last_name: Özgen
- first_name: Alexis
full_name: Ivec, Alexis
id: 1d144691-e8be-11ed-9b33-bdd3077fad4c
last_name: Ivec
- first_name: Christoph
full_name: Bock, Christoph
last_name: Bock
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Simon
full_name: Hippenmeyer, Simon
id: 37B36620-F248-11E8-B48F-1D18A9856A87
last_name: Hippenmeyer
orcid: 0000-0003-2279-1061
citation:
ama: Cheung GT, Pauler F, Koppensteiner P, et al. Multipotent progenitors instruct
ontogeny of the superior colliculus. Neuron. 2024;112(2):230-246.e11. doi:10.1016/j.neuron.2023.11.009
apa: Cheung, G. T., Pauler, F., Koppensteiner, P., Krausgruber, T., Streicher, C.,
Schrammel, M., … Hippenmeyer, S. (2024). Multipotent progenitors instruct ontogeny
of the superior colliculus. Neuron. Elsevier. https://doi.org/10.1016/j.neuron.2023.11.009
chicago: Cheung, Giselle T, Florian Pauler, Peter Koppensteiner, Thomas Krausgruber,
Carmen Streicher, Martin Schrammel, Natalie Y Özgen, et al. “Multipotent Progenitors
Instruct Ontogeny of the Superior Colliculus.” Neuron. Elsevier, 2024.
https://doi.org/10.1016/j.neuron.2023.11.009.
ieee: G. T. Cheung et al., “Multipotent progenitors instruct ontogeny of
the superior colliculus,” Neuron, vol. 112, no. 2. Elsevier, p. 230–246.e11,
2024.
ista: Cheung GT, Pauler F, Koppensteiner P, Krausgruber T, Streicher C, Schrammel
M, Özgen NY, Ivec A, Bock C, Shigemoto R, Hippenmeyer S. 2024. Multipotent progenitors
instruct ontogeny of the superior colliculus. Neuron. 112(2), 230–246.e11.
mla: Cheung, Giselle T., et al. “Multipotent Progenitors Instruct Ontogeny of the
Superior Colliculus.” Neuron, vol. 112, no. 2, Elsevier, 2024, p. 230–246.e11,
doi:10.1016/j.neuron.2023.11.009.
short: G.T. Cheung, F. Pauler, P. Koppensteiner, T. Krausgruber, C. Streicher, M.
Schrammel, N.Y. Özgen, A. Ivec, C. Bock, R. Shigemoto, S. Hippenmeyer, Neuron
112 (2024) 230–246.e11.
corr_author: '1'
date_created: 2023-04-27T09:41:48Z
date_published: 2024-01-17T00:00:00Z
date_updated: 2025-12-30T10:54:12Z
day: '17'
ddc:
- '570'
department:
- _id: SiHi
- _id: RySh
doi: 10.1016/j.neuron.2023.11.009
external_id:
isi:
- '001163937900001'
pmid:
- '38096816'
file:
- access_level: open_access
checksum: 32b3788f7085cf44a84108d8faaff3ce
content_type: application/pdf
creator: dernst
date_created: 2024-02-06T13:56:15Z
date_updated: 2024-02-06T13:56:15Z
file_id: '14944'
file_name: 2024_Neuron_Cheung.pdf
file_size: 5942467
relation: main_file
success: 1
file_date_updated: 2024-02-06T13:56:15Z
fulldoi: https://doi.org/10.1016/j.neuron.2023.11.009
has_accepted_license: '1'
intvolume: ' 112'
isi: 1
issue: '2'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
page: 230-246.e11
pmid: 1
project:
- _id: 059F6AB4-7A3F-11EA-A408-12923DDC885E
grant_number: F7805
name: Stem Cell Modulation in Neural Development and Regeneration/ P05-Molecular
Mechanisms of Neural Stem Cell Lineage Progression
publication: Neuron
publication_identifier:
issn:
- 0896-6273
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
link:
- description: News on ISTA Website
relation: press_release
url: https://ista.ac.at/en/news/the-pedigree-of-brain-cells/
scopus_import: '1'
status: public
title: Multipotent progenitors instruct ontogeny of the superior colliculus
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 112
year: '2024'
...
---
_id: '14683'
abstract:
- lang: eng
text: "Mosaic analysis with double markers (MADM) technology enables the generation
of genetic mosaic tissue in mice and high-resolution phenotyping at the individual
cell level. Here, we present a protocol for isolating MADM-labeled cells with
high yield for downstream molecular analyses using fluorescence-activated cell
sorting (FACS). We describe steps for generating MADM-labeled mice, perfusion,
single-cell suspension, and debris removal. We then detail procedures for cell
sorting by FACS and downstream analysis. This protocol is suitable for embryonic
to adult mice.\r\nFor complete details on the use and execution of this protocol,
please refer to Contreras et al. (2021).1"
acknowledged_ssus:
- _id: Bio
- _id: PreCl
acknowledgement: This research was supported by the Scientific Service Units (SSU)
at IST Austria through resources provided by the Imaging & Optics Facility (IOF)
and Preclinical Facilities (PCF). N.A. received support from FWF Firnberg-Programme
(T 1031). G.C. received support from the European Union’s Horizon 2020 research
and innovation programme under the Marie Skłodowska-Curie grant agreement no. 754411
as an ISTplus postdoctoral fellow. This work was also supported by IST Austria institutional
funds, FWF SFB F78 to S.H., and the European Research Council (ERC) under the European
Union’s Horizon 2020 research and innovation programme (grant agreement no. 725780
LinPro) to S.H.
article_number: '102771'
article_processing_charge: Yes (in subscription journal)
article_type: review
author:
- first_name: Nicole
full_name: Amberg, Nicole
id: 4CD6AAC6-F248-11E8-B48F-1D18A9856A87
last_name: Amberg
orcid: 0000-0002-3183-8207
- first_name: Giselle T
full_name: Cheung, Giselle T
id: 471195F6-F248-11E8-B48F-1D18A9856A87
last_name: Cheung
orcid: 0000-0001-8457-2572
- first_name: Simon
full_name: Hippenmeyer, Simon
id: 37B36620-F248-11E8-B48F-1D18A9856A87
last_name: Hippenmeyer
orcid: 0000-0003-2279-1061
citation:
ama: Amberg N, Cheung GT, Hippenmeyer S. Protocol for sorting cells from mouse brains
labeled with mosaic analysis with double markers by flow cytometry. STAR Protocols.
2024;5(1). doi:10.1016/j.xpro.2023.102771
apa: Amberg, N., Cheung, G. T., & Hippenmeyer, S. (2024). Protocol for sorting
cells from mouse brains labeled with mosaic analysis with double markers by flow
cytometry. STAR Protocols. Elsevier. https://doi.org/10.1016/j.xpro.2023.102771
chicago: Amberg, Nicole, Giselle T Cheung, and Simon Hippenmeyer. “Protocol for
Sorting Cells from Mouse Brains Labeled with Mosaic Analysis with Double Markers
by Flow Cytometry.” STAR Protocols. Elsevier, 2024. https://doi.org/10.1016/j.xpro.2023.102771.
ieee: N. Amberg, G. T. Cheung, and S. Hippenmeyer, “Protocol for sorting cells from
mouse brains labeled with mosaic analysis with double markers by flow cytometry,”
STAR Protocols, vol. 5, no. 1. Elsevier, 2024.
ista: Amberg N, Cheung GT, Hippenmeyer S. 2024. Protocol for sorting cells from
mouse brains labeled with mosaic analysis with double markers by flow cytometry.
STAR Protocols. 5(1), 102771.
mla: Amberg, Nicole, et al. “Protocol for Sorting Cells from Mouse Brains Labeled
with Mosaic Analysis with Double Markers by Flow Cytometry.” STAR Protocols,
vol. 5, no. 1, 102771, Elsevier, 2024, doi:10.1016/j.xpro.2023.102771.
short: N. Amberg, G.T. Cheung, S. Hippenmeyer, STAR Protocols 5 (2024).
corr_author: '1'
date_created: 2023-12-13T11:48:05Z
date_published: 2024-03-15T00:00:00Z
date_updated: 2025-04-15T08:23:06Z
day: '15'
ddc:
- '570'
department:
- _id: SiHi
doi: 10.1016/j.xpro.2023.102771
ec_funded: 1
external_id:
pmid:
- '38070137'
file:
- access_level: open_access
checksum: 3f0ee62e04bf5a44b45b035662826e95
content_type: application/pdf
creator: dernst
date_created: 2024-07-16T11:50:03Z
date_updated: 2024-07-16T11:50:03Z
file_id: '17260'
file_name: 2024_STARProtoc_Amberg.pdf
file_size: 8871807
relation: main_file
success: 1
file_date_updated: 2024-07-16T11:50:03Z
fulldoi: https://doi.org/10.1016/j.xpro.2023.102771
has_accepted_license: '1'
intvolume: ' 5'
issue: '1'
keyword:
- General Immunology and Microbiology
- General Biochemistry
- Genetics and Molecular Biology
- General Neuroscience
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 268F8446-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: T01031
name: Role of Eed in neural stem cell lineage progression
- _id: 260C2330-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '754411'
name: ISTplus - Postdoctoral Fellowships
- _id: 059F6AB4-7A3F-11EA-A408-12923DDC885E
grant_number: F7805
name: Stem Cell Modulation in Neural Development and Regeneration/ P05-Molecular
Mechanisms of Neural Stem Cell Lineage Progression
- _id: 260018B0-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '725780'
name: Principles of Neural Stem Cell Lineage Progression in Cerebral Cortex Development
publication: STAR Protocols
publication_identifier:
issn:
- 2666-1667
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Protocol for sorting cells from mouse brains labeled with mosaic analysis with
double markers by flow cytometry
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 5
year: '2024'
...
---
_id: '14794'
abstract:
- lang: eng
text: "Mosaic analysis with double markers (MADM) technology enables the sparse
labeling of genetically defined neurons. We present a protocol for time-lapse
imaging of cortical projection neuron migration in mice using MADM. We describe
steps for the isolation, culturing, and 4D imaging of neuronal dynamics in MADM-labeled
brain tissue. While this protocol is compatible with other single-cell labeling
methods, the MADM approach provides a genetic platform for the functional assessment
of cell-autonomous candidate gene function and the relative contribution of non-cell-autonomous
effects.\r\n\r\nFor complete details on the use and execution of this protocol,
please refer to Hansen et al. (2022),1 Contreras et al. (2021),2 and Amberg and
Hippenmeyer (2021).3"
acknowledged_ssus:
- _id: Bio
- _id: PreCl
acknowledgement: We thank Florian Pauler for discussion and his expert technical support.
This research was supported by the Scientific Service Units (SSU) at IST Austria
through resources provided by the Imaging and Optics Facility (IOF) and Preclinical
Facility (PCF). A.H.H. was a recipient of a DOC Fellowship (24812) of the Austrian
Academy of Sciences.
article_number: '102795'
article_processing_charge: Yes
article_type: review
author:
- first_name: Andi H
full_name: Hansen, Andi H
id: 38853E16-F248-11E8-B48F-1D18A9856A87
last_name: Hansen
- first_name: Simon
full_name: Hippenmeyer, Simon
id: 37B36620-F248-11E8-B48F-1D18A9856A87
last_name: Hippenmeyer
orcid: 0000-0003-2279-1061
citation:
ama: Hansen AH, Hippenmeyer S. Time-lapse imaging of cortical projection neuron
migration in mice using mosaic analysis with double markers. STAR Protocols.
2024;5(1). doi:10.1016/j.xpro.2023.102795
apa: Hansen, A. H., & Hippenmeyer, S. (2024). Time-lapse imaging of cortical
projection neuron migration in mice using mosaic analysis with double markers.
STAR Protocols. Elsevier. https://doi.org/10.1016/j.xpro.2023.102795
chicago: Hansen, Andi H, and Simon Hippenmeyer. “Time-Lapse Imaging of Cortical
Projection Neuron Migration in Mice Using Mosaic Analysis with Double Markers.”
STAR Protocols. Elsevier, 2024. https://doi.org/10.1016/j.xpro.2023.102795.
ieee: A. H. Hansen and S. Hippenmeyer, “Time-lapse imaging of cortical projection
neuron migration in mice using mosaic analysis with double markers,” STAR Protocols,
vol. 5, no. 1. Elsevier, 2024.
ista: Hansen AH, Hippenmeyer S. 2024. Time-lapse imaging of cortical projection
neuron migration in mice using mosaic analysis with double markers. STAR Protocols.
5(1), 102795.
mla: Hansen, Andi H., and Simon Hippenmeyer. “Time-Lapse Imaging of Cortical Projection
Neuron Migration in Mice Using Mosaic Analysis with Double Markers.” STAR Protocols,
vol. 5, no. 1, 102795, Elsevier, 2024, doi:10.1016/j.xpro.2023.102795.
short: A.H. Hansen, S. Hippenmeyer, STAR Protocols 5 (2024).
corr_author: '1'
date_created: 2024-01-14T23:00:56Z
date_published: 2024-03-15T00:00:00Z
date_updated: 2025-04-15T07:32:40Z
day: '15'
ddc:
- '570'
department:
- _id: SiHi
doi: 10.1016/j.xpro.2023.102795
external_id:
pmid:
- '38165800'
file:
- access_level: open_access
checksum: 4644d537451c5c114a9d7c7829b65bba
content_type: application/pdf
creator: dernst
date_created: 2024-07-16T12:04:46Z
date_updated: 2024-07-16T12:04:46Z
file_id: '17264'
file_name: 2024_STARProtoc_Hansen.pdf
file_size: 3758943
relation: main_file
success: 1
file_date_updated: 2024-07-16T12:04:46Z
fulldoi: https://doi.org/10.1016/j.xpro.2023.102795
has_accepted_license: '1'
intvolume: ' 5'
issue: '1'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 2625A13E-B435-11E9-9278-68D0E5697425
grant_number: '24812'
name: Molecular mechanisms of radial neuronal migration
publication: STAR Protocols
publication_identifier:
eissn:
- 2666-1667
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
link:
- relation: software
url: http://github.com/hippenmeyerlab
scopus_import: '1'
status: public
title: Time-lapse imaging of cortical projection neuron migration in mice using mosaic
analysis with double markers
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 5
year: '2024'
...
---
_id: '15385'
abstract:
- lang: eng
text: "Relevant information about the data can be found in the 'Readme_Data.txt'
file. \r\nA previous version of the publication can be found on BioRxiv: https://www.biorxiv.org/content/10.1101/2022.10.11.511691v4\r\nand
published in Plos Biology (2024)"
acknowledged_ssus:
- _id: PreCl
- _id: M-Shop
- _id: LifeSc
- _id: Bio
acknowledgement: 'We thank Armel Nicolas, Bella Bruszel and Ewelina Dutkiewicz from
the ISTA Mass Spectrometry Service (Lab Services Facilities) for all Proteomics
work, including samples preparation, LC/MS data acquisition, searches and data evaluation.
We thank Prof. Peter Jonas for his suggestion on the involvement of potassium channels
and members of the Neuroethology group for their comments on the manuscript. Katalin
Szigeti and Julie Murmann for experimental help. This research was supported by
the Scientific Service Units of ISTA through resources provided by the Lab Support
Facility, the Imaging and Optics Facility, the Machine Shop Unit and the Preclinical
Facility, especially Freyja Langer and Michael Schunn. '
article_processing_charge: No
author:
- first_name: Laura
full_name: Burnett, Laura
id: 3B717F68-F248-11E8-B48F-1D18A9856A87
last_name: Burnett
orcid: 0000-0002-8937-410X
- first_name: Peter
full_name: Koppensteiner, Peter
id: 3B8B25A8-F248-11E8-B48F-1D18A9856A87
last_name: Koppensteiner
orcid: 0000-0002-3509-1948
- first_name: Olga
full_name: Symonova, Olga
id: 3C0C7BC6-F248-11E8-B48F-1D18A9856A87
last_name: Symonova
orcid: 0000-0003-2012-9947
- first_name: Tomas
full_name: Masson, Tomas
id: 93ac43e8-8599-11eb-9b86-f6efb0a4c207
last_name: Masson
orcid: 0000-0002-2634-6283
- first_name: Tomas A
full_name: Vega Zuniga, Tomas A
id: 2E7C4E78-F248-11E8-B48F-1D18A9856A87
last_name: Vega Zuniga
- first_name: Ximena
full_name: Contreras, Ximena
id: 475990FE-F248-11E8-B48F-1D18A9856A87
last_name: Contreras
- first_name: Thomas
full_name: Rülicke, Thomas
last_name: Rülicke
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Gaia
full_name: Novarino, Gaia
id: 3E57A680-F248-11E8-B48F-1D18A9856A87
last_name: Novarino
orcid: 0000-0002-7673-7178
- first_name: Maximilian A
full_name: Jösch, Maximilian A
id: 2BD278E6-F248-11E8-B48F-1D18A9856A87
last_name: Jösch
orcid: 0000-0002-3937-1330
citation:
ama: Burnett L, Koppensteiner P, Symonova O, et al. Shared behavioural impairments
in visual perception and place avoidance across different autism models are driven
by periaqueductal grey hypoexcitability in Setd5 haploinsufficient mice. 2024.
doi:10.15479/AT:ISTA:15385
apa: Burnett, L., Koppensteiner, P., Symonova, O., Masson, T., Vega Zuniga, T. A.,
Contreras, X., … Jösch, M. A. (2024). Shared behavioural impairments in visual
perception and place avoidance across different autism models are driven by periaqueductal
grey hypoexcitability in Setd5 haploinsufficient mice. Institute of Science and
Technology Austria. https://doi.org/10.15479/AT:ISTA:15385
chicago: Burnett, Laura, Peter Koppensteiner, Olga Symonova, Tomas Masson, Tomas
A Vega Zuniga, Ximena Contreras, Thomas Rülicke, Ryuichi Shigemoto, Gaia Novarino,
and Maximilian A Jösch. “Shared Behavioural Impairments in Visual Perception and
Place Avoidance across Different Autism Models Are Driven by Periaqueductal Grey
Hypoexcitability in Setd5 Haploinsufficient Mice.” Institute of Science and Technology
Austria, 2024. https://doi.org/10.15479/AT:ISTA:15385.
ieee: L. Burnett et al., “Shared behavioural impairments in visual perception
and place avoidance across different autism models are driven by periaqueductal
grey hypoexcitability in Setd5 haploinsufficient mice.” Institute of Science and
Technology Austria, 2024.
ista: Burnett L, Koppensteiner P, Symonova O, Masson T, Vega Zuniga TA, Contreras
X, Rülicke T, Shigemoto R, Novarino G, Jösch MA. 2024. Shared behavioural impairments
in visual perception and place avoidance across different autism models are driven
by periaqueductal grey hypoexcitability in Setd5 haploinsufficient mice, Institute
of Science and Technology Austria, 10.15479/AT:ISTA:15385.
mla: Burnett, Laura, et al. Shared Behavioural Impairments in Visual Perception
and Place Avoidance across Different Autism Models Are Driven by Periaqueductal
Grey Hypoexcitability in Setd5 Haploinsufficient Mice. Institute of Science
and Technology Austria, 2024, doi:10.15479/AT:ISTA:15385.
short: L. Burnett, P. Koppensteiner, O. Symonova, T. Masson, T.A. Vega Zuniga, X.
Contreras, T. Rülicke, R. Shigemoto, G. Novarino, M.A. Jösch, (2024).
corr_author: '1'
date_created: 2024-05-13T15:04:04Z
date_published: 2024-05-15T00:00:00Z
date_updated: 2025-09-08T07:57:11Z
day: '15'
ddc:
- '570'
department:
- _id: MaJö
- _id: PreCl
- _id: SiHi
- _id: RySh
- _id: GaNo
doi: 10.15479/AT:ISTA:15385
file:
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success: 1
file_date_updated: 2024-05-16T09:08:20Z
fulldoi: https://doi.org/10.15479/AT:ISTA:15385
has_accepted_license: '1'
keyword:
- ASD
- periaqueductal gray
- perception
- behavior
- potassium channels
month: '05'
oa: 1
oa_version: Published Version
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '17142'
relation: used_in_publication
status: public
status: public
title: Shared behavioural impairments in visual perception and place avoidance across
different autism models are driven by periaqueductal grey hypoexcitability in Setd5
haploinsufficient mice
tmp:
image: /images/cc_by_nc.png
legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode
name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
short: CC BY-NC (4.0)
type: research_data
user_id: 68b8ca59-c5b3-11ee-8790-cd641c68093d
year: '2024'
...
---
APC_amount: 804 EUR
OA_place: publisher
OA_type: gold
_id: '17187'
abstract:
- lang: eng
text: "The generation of diverse cell types during development is fundamental to
brain\r\nfunctions. We outline a protocol to quantitatively assess the clonal
output of individual neural progenitors using mosaic analysis with double markers
(MADM) in\r\nmice. We first describe steps to acquire and reconstruct adult MADM
clones in\r\nthe superior colliculus. Then we detail analysis pipelines to determine
clonal\r\ncomposition and architecture. This protocol enables the buildup of quantitative\r\nframeworks
of lineage progression with precise spatial resolution in the brain.\r\nFor complete
details on the use and execution of this protocol, please refer to\r\nCheung et
al.1"
acknowledged_ssus:
- _id: Bio
- _id: PreCl
acknowledgement: We thank A. Heger for mouse breeding support. This work was supported
by the Scientific Service Units of IST Austria through resources provided by the
Imaging & Optics and Preclinical facilities. G.C. received funding from the European
Commission (IST plus postdoctoral fellowship); S.H. was funded by ISTA institutional
funds and the Austrian Science Fund Special Research Programmes (FWF SFB-F78 Neuro
Stem Modulation).
article_number: '103157'
article_processing_charge: Yes
article_type: original
author:
- first_name: Giselle T
full_name: Cheung, Giselle T
id: 471195F6-F248-11E8-B48F-1D18A9856A87
last_name: Cheung
orcid: 0000-0001-8457-2572
- first_name: Carmen
full_name: Streicher, Carmen
id: 36BCB99C-F248-11E8-B48F-1D18A9856A87
last_name: Streicher
- first_name: Simon
full_name: Hippenmeyer, Simon
id: 37B36620-F248-11E8-B48F-1D18A9856A87
last_name: Hippenmeyer
orcid: 0000-0003-2279-1061
citation:
ama: Cheung GT, Streicher C, Hippenmeyer S. Protocol for quantitative reconstruction
of cell lineage using mosaic analysis with double markers in mice. STAR Protocols.
2024;5(3). doi:10.1016/j.xpro.2024.103157
apa: Cheung, G. T., Streicher, C., & Hippenmeyer, S. (2024). Protocol for quantitative
reconstruction of cell lineage using mosaic analysis with double markers in mice.
STAR Protocols. Elsevier. https://doi.org/10.1016/j.xpro.2024.103157
chicago: Cheung, Giselle T, Carmen Streicher, and Simon Hippenmeyer. “Protocol for
Quantitative Reconstruction of Cell Lineage Using Mosaic Analysis with Double
Markers in Mice.” STAR Protocols. Elsevier, 2024. https://doi.org/10.1016/j.xpro.2024.103157.
ieee: G. T. Cheung, C. Streicher, and S. Hippenmeyer, “Protocol for quantitative
reconstruction of cell lineage using mosaic analysis with double markers in mice,”
STAR Protocols, vol. 5, no. 3. Elsevier, 2024.
ista: Cheung GT, Streicher C, Hippenmeyer S. 2024. Protocol for quantitative reconstruction
of cell lineage using mosaic analysis with double markers in mice. STAR Protocols.
5(3), 103157.
mla: Cheung, Giselle T., et al. “Protocol for Quantitative Reconstruction of Cell
Lineage Using Mosaic Analysis with Double Markers in Mice.” STAR Protocols,
vol. 5, no. 3, 103157, Elsevier, 2024, doi:10.1016/j.xpro.2024.103157.
short: G.T. Cheung, C. Streicher, S. Hippenmeyer, STAR Protocols 5 (2024).
corr_author: '1'
date_created: 2024-06-30T22:01:04Z
date_published: 2024-09-20T00:00:00Z
date_updated: 2025-12-30T10:54:11Z
day: '20'
ddc:
- '570'
department:
- _id: SiHi
doi: 10.1016/j.xpro.2024.103157
ec_funded: 1
external_id:
pmid:
- '38935508'
file:
- access_level: open_access
checksum: d8a8cdba82a394e731aa699ace1ae433
content_type: application/pdf
creator: dernst
date_created: 2025-01-09T12:12:40Z
date_updated: 2025-01-09T12:12:40Z
file_id: '18809'
file_name: 2024_STARProtoc_Cheung.pdf
file_size: 5186071
relation: main_file
success: 1
file_date_updated: 2025-01-09T12:12:40Z
fulldoi: https://doi.org/10.1016/j.xpro.2024.103157
has_accepted_license: '1'
intvolume: ' 5'
issue: '3'
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 260C2330-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '754411'
name: ISTplus - Postdoctoral Fellowships
- _id: 059F6AB4-7A3F-11EA-A408-12923DDC885E
grant_number: F7805
name: Stem Cell Modulation in Neural Development and Regeneration/ P05-Molecular
Mechanisms of Neural Stem Cell Lineage Progression
publication: STAR Protocols
publication_identifier:
eissn:
- 2666-1667
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Protocol for quantitative reconstruction of cell lineage using mosaic analysis
with double markers in mice
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 5
year: '2024'
...
---
APC_amount: 804 EUR
OA_place: publisher
OA_type: gold
_id: '17232'
abstract:
- lang: eng
text: "The lineage relationship of clonally-related cells offers important insights
into the ontogeny and cytoarchitecture of the brain in health and disease. Here,
we provide a protocol to concurrently assess cell lineage relationship and cell-type
identity among clonally-related cells in situ. We first describe the preparation
and screening of acute brain slices containing clonally-related cells labeled
using mosaic analysis with double markers (MADM). We then outline steps to collect
RNA from individual cells for downstream applications and cell-type identification
using RNA sequencing.\r\nFor complete details on the use and execution of this
protocol, please refer to Cheung et al.\r\n1"
acknowledged_ssus:
- _id: Bio
- _id: M-Shop
- _id: PreCl
acknowledgement: We thank R. Beattie and T. Asenov for designing and producing components
of the multi-well slice recover chamber. We thank R. Shigemoto for providing equipment
access. We thank C. Streicher and A. Heger for mouse breeding support. This work
was supported by the Scientific Service Units of IST Austria through resources provided
by the Imaging & Optics, Miba Machine Shop, and Preclinical facilities. G.C. received
funding from the European Commission (IST plus postdoctoral fellowship) and S.H.
was funded by ISTA institutional funds and the Austrian Science Fund Special Research
Programmes (FWF SFB-F78 Neuro Stem Modulation).
article_number: '103168'
article_processing_charge: Yes
article_type: original
author:
- first_name: Giselle T
full_name: Cheung, Giselle T
id: 471195F6-F248-11E8-B48F-1D18A9856A87
last_name: Cheung
orcid: 0000-0001-8457-2572
- first_name: Florian
full_name: Pauler, Florian
id: 48EA0138-F248-11E8-B48F-1D18A9856A87
last_name: Pauler
orcid: 0000-0002-7462-0048
- first_name: Peter
full_name: Koppensteiner, Peter
id: 3B8B25A8-F248-11E8-B48F-1D18A9856A87
last_name: Koppensteiner
orcid: 0000-0002-3509-1948
- first_name: Simon
full_name: Hippenmeyer, Simon
id: 37B36620-F248-11E8-B48F-1D18A9856A87
last_name: Hippenmeyer
orcid: 0000-0003-2279-1061
citation:
ama: Cheung GT, Pauler F, Koppensteiner P, Hippenmeyer S. Protocol for mapping cell
lineage and cell-type identity of clonally-related cells in situ using MADM-CloneSeq.
STAR Protocols. 2024;5(3). doi:10.1016/j.xpro.2024.103168
apa: Cheung, G. T., Pauler, F., Koppensteiner, P., & Hippenmeyer, S. (2024).
Protocol for mapping cell lineage and cell-type identity of clonally-related cells
in situ using MADM-CloneSeq. STAR Protocols. Elsevier. https://doi.org/10.1016/j.xpro.2024.103168
chicago: Cheung, Giselle T, Florian Pauler, Peter Koppensteiner, and Simon Hippenmeyer.
“Protocol for Mapping Cell Lineage and Cell-Type Identity of Clonally-Related
Cells in Situ Using MADM-CloneSeq.” STAR Protocols. Elsevier, 2024. https://doi.org/10.1016/j.xpro.2024.103168.
ieee: G. T. Cheung, F. Pauler, P. Koppensteiner, and S. Hippenmeyer, “Protocol for
mapping cell lineage and cell-type identity of clonally-related cells in situ
using MADM-CloneSeq,” STAR Protocols, vol. 5, no. 3. Elsevier, 2024.
ista: Cheung GT, Pauler F, Koppensteiner P, Hippenmeyer S. 2024. Protocol for mapping
cell lineage and cell-type identity of clonally-related cells in situ using MADM-CloneSeq.
STAR Protocols. 5(3), 103168.
mla: Cheung, Giselle T., et al. “Protocol for Mapping Cell Lineage and Cell-Type
Identity of Clonally-Related Cells in Situ Using MADM-CloneSeq.” STAR Protocols,
vol. 5, no. 3, 103168, Elsevier, 2024, doi:10.1016/j.xpro.2024.103168.
short: G.T. Cheung, F. Pauler, P. Koppensteiner, S. Hippenmeyer, STAR Protocols
5 (2024).
corr_author: '1'
date_created: 2024-07-14T22:01:10Z
date_published: 2024-09-20T00:00:00Z
date_updated: 2025-12-30T10:54:12Z
day: '20'
ddc:
- '570'
department:
- _id: SiHi
- _id: PreCl
doi: 10.1016/j.xpro.2024.103168
external_id:
pmid:
- '38968076'
file:
- access_level: open_access
checksum: 464f52ecc6ec92f509552823bb82bf79
content_type: application/pdf
creator: dernst
date_created: 2025-01-09T12:16:53Z
date_updated: 2025-01-09T12:16:53Z
file_id: '18810'
file_name: 2024_STARProtoc_Cheung2.pdf
file_size: 6445556
relation: main_file
success: 1
file_date_updated: 2025-01-09T12:16:53Z
fulldoi: https://doi.org/10.1016/j.xpro.2024.103168
has_accepted_license: '1'
intvolume: ' 5'
issue: '3'
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 059F6AB4-7A3F-11EA-A408-12923DDC885E
grant_number: F7805
name: Stem Cell Modulation in Neural Development and Regeneration/ P05-Molecular
Mechanisms of Neural Stem Cell Lineage Progression
publication: STAR Protocols
publication_identifier:
eissn:
- 2666-1667
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Protocol for mapping cell lineage and cell-type identity of clonally-related
cells in situ using MADM-CloneSeq
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 5
year: '2024'
...
---
_id: '17425'
abstract:
- lang: eng
text: Mosaic Analysis with Double Markers (MADM) is a powerful genetic method typically
used for lineage tracing and to disentangle cell autonomous and tissue-wide roles
of candidate genes with single cell resolution. Given the relatively sparse labeling,
depending on which of the 19 MADM chromosomes one chooses, the MADM approach represents
the perfect opportunity for cell morphology analysis. Various MADM studies include
reports of morphological anomalies and phenotypes in the central nervous system
(CNS). MADM for any candidate gene can easily incorporate morphological analysis
within the experimental workflow. Here, we describe the methods of morphological
cell analysis which we developed in the course of diverse recent MADM studies.
This chapter will specifically focus on methods to quantify aspects of the morphology
of neurons and astrocytes within the CNS, but these methods can broadly be applied
to any MADM-labeled cells throughout the entire organism. We will cover two analyses—soma
volume and dendrite characterization—of physical characteristics of pyramidal
neurons in the somatosensory cortex, and two analyses—volume and Sholl analysis—of
astrocyte morphology.
acknowledged_ssus:
- _id: Bio
acknowledgement: We thank all Hippenmeyer lab members for support and discussions.
This work was supported by the Scientific Service Units (SSU) at ISTA through resources
provided by the Imaging & Optics Facility (IOF). O.A.M was a recipient of a DOC
Fellowship (26253) of the Austrian Academy of Sciences. This work was supported
by ISTA institutional funds, and The Austrian Science Fund Special Research Programmes
(FWF SFB F78 Neuro Stem Modulation) to S.H.
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Osvaldo
full_name: Miranda, Osvaldo
id: 862A3C56-A8BF-11E9-B4FA-D9E3E5697425
last_name: Miranda
orcid: 0000-0001-6618-6889
- first_name: Giselle T
full_name: Cheung, Giselle T
id: 471195F6-F248-11E8-B48F-1D18A9856A87
last_name: Cheung
orcid: 0000-0001-8457-2572
- first_name: Simon
full_name: Hippenmeyer, Simon
id: 37B36620-F248-11E8-B48F-1D18A9856A87
last_name: Hippenmeyer
orcid: 0000-0003-2279-1061
citation:
ama: 'Miranda O, Cheung GT, Hippenmeyer S. Morphological Analysis of Neurons and
Glia Using Mosaic Analysis with Double Markers. In: Toyooka K, ed. Neuronal
Morphogenesis. Vol 2831. 1st ed. MIMB. New York, NY: Springer Nature; 2024:283-299.
doi:10.1007/978-1-0716-3969-6_19'
apa: 'Miranda, O., Cheung, G. T., & Hippenmeyer, S. (2024). Morphological Analysis
of Neurons and Glia Using Mosaic Analysis with Double Markers. In K. Toyooka (Ed.),
Neuronal Morphogenesis (1st ed., Vol. 2831, pp. 283–299). New York, NY:
Springer Nature. https://doi.org/10.1007/978-1-0716-3969-6_19'
chicago: 'Miranda, Osvaldo, Giselle T Cheung, and Simon Hippenmeyer. “Morphological
Analysis of Neurons and Glia Using Mosaic Analysis with Double Markers.” In Neuronal
Morphogenesis, edited by Kazuhito Toyooka, 1st ed., 2831:283–99. MIMB. New
York, NY: Springer Nature, 2024. https://doi.org/10.1007/978-1-0716-3969-6_19.'
ieee: 'O. Miranda, G. T. Cheung, and S. Hippenmeyer, “Morphological Analysis of
Neurons and Glia Using Mosaic Analysis with Double Markers,” in Neuronal Morphogenesis,
1st ed., vol. 2831, K. Toyooka, Ed. New York, NY: Springer Nature, 2024, pp. 283–299.'
ista: 'Miranda O, Cheung GT, Hippenmeyer S. 2024.Morphological Analysis of Neurons
and Glia Using Mosaic Analysis with Double Markers. In: Neuronal Morphogenesis.
Methods in Molecular Biology, vol. 2831, 283–299.'
mla: Miranda, Osvaldo, et al. “Morphological Analysis of Neurons and Glia Using
Mosaic Analysis with Double Markers.” Neuronal Morphogenesis, edited by
Kazuhito Toyooka, 1st ed., vol. 2831, Springer Nature, 2024, pp. 283–99, doi:10.1007/978-1-0716-3969-6_19.
short: O. Miranda, G.T. Cheung, S. Hippenmeyer, in:, K. Toyooka (Ed.), Neuronal
Morphogenesis, 1st ed., Springer Nature, New York, NY, 2024, pp. 283–299.
corr_author: '1'
date_created: 2024-08-13T12:16:41Z
date_published: 2024-08-13T00:00:00Z
date_updated: 2026-09-13T22:30:11Z
day: '13'
department:
- _id: GradSch
- _id: SiHi
doi: 10.1007/978-1-0716-3969-6_19
edition: '1'
editor:
- first_name: Kazuhito
full_name: Toyooka, Kazuhito
last_name: Toyooka
external_id:
pmid:
- '39134857'
fulldoi: https://doi.org/10.1007/978-1-0716-3969-6_19
intvolume: ' 2831'
language:
- iso: eng
month: '08'
oa_version: None
page: 283-299
place: New York, NY
pmid: 1
project:
- _id: 34c9fbcb-11ca-11ed-8bc3-98fa5658610d
grant_number: '26253'
name: Molecular Mechanisms Regulating Cortical Neural Stem Cell Lineage Progression
and Astrocyte Development
- _id: 059F6AB4-7A3F-11EA-A408-12923DDC885E
grant_number: F7805
name: Stem Cell Modulation in Neural Development and Regeneration/ P05-Molecular
Mechanisms of Neural Stem Cell Lineage Progression
publication: Neuronal Morphogenesis
publication_identifier:
eisbn:
- '9781071639696'
eissn:
- 1940-6029
isbn:
- '9781071639689'
issn:
- 1064-3745
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
record:
- id: '20212'
relation: dissertation_contains
status: public
scopus_import: '1'
series_title: MIMB
status: public
title: Morphological Analysis of Neurons and Glia Using Mosaic Analysis with Double
Markers
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 2831
year: '2024'
...