---
res:
  bibo_abstract:
  - "Plant motions occur across a wide spectrum of timescales, ranging from seed dispersal
    through bursting (milliseconds) and stomatal opening (minutes) to long-term adaptation
    of gross architecture. Relatively fast motions include water-driven growth as
    exemplified by root cell expansion under abiotic/biotic stresses or during gravitropism.
    A showcase is a root growth inhibition in 30 seconds triggered by the phytohormone
    auxin. However, the cellular and molecular mechanisms are still largely unknown.
    This thesis covers the studies about this topic as follows. By taking advantage
    of microfluidics combined with live imaging, pharmaceutical tools, and transgenic
    lines, we examined the kinetics of and causal relationship among various auxininduced
    rapid cellular changes in root growth, apoplastic pH, cytosolic Ca2+, cortical
    microtubule (CMT) orientation, and vacuolar morphology. We revealed that CMT reorientation
    and vacuolar constriction are the consequence of growth itself instead of responding
    directly to auxin. In contrast, auxin induces apoplast alkalinization to rapidly
    inhibit root growth in 30 seconds. This auxin-triggered apoplast alkalinization
    results from rapid H+- influx that is contributed by Ca2+ inward channel CYCLIC
    NUCLEOTIDE-GATED CHANNEL 14 (CNGC14)-dependent Ca2+ signaling. To dissect which
    auxin signaling mediates the rapid apoplast alkalinization, we\r\ncombined microfluidics
    and genetic engineering to verify that TIR1/AFB receptors conduct a non-transcriptional
    regulation on Ca2+ and H+ -influx. This non-canonical pathway is mostly mediated
    by the cytosolic portion of TIR1/AFB. On the other hand, we uncovered, using biochemical
    and phospho-proteomic analysis, that auxin cell surface signaling component TRANSMEMBRANE
    KINASE 1 (TMK1) plays a negative role during auxin-trigger apoplast\r\nalkalinization
    and root growth inhibition through directly activating PM H+ -ATPases. Therefore,
    we discovered that PM H+ -ATPases counteract instead of mediate the auxintriggered
    rapid H+ -influx, and that TIR1/AFB and TMK1 regulate root growth antagonistically.
    This opposite effect of TIR1/AFB and TMK1 is consistent during auxin-induced hypocotyl
    elongation, leading us to explore the relation of two signaling pathways. Assisted
    with biochemistry and fluorescent imaging, we verified for the first time that
    TIR1/AFB and TMK1 can interact with each other. The ability of TIR1/AFB binding
    to membrane lipid provides a basis for the interaction of plasma membrane- and
    cytosol-localized proteins.\r\nBesides, transgenic analysis combined with genetic
    engineering and biochemistry showed that  vi\r\nthey do function in the same pathway.
    Particularly, auxin-induced TMK1 increase is TIR1/AFB dependent, suggesting TIR1/AFB
    regulation on TMK1. Conversely, TMK1 also regulates TIR1/AFB protein levels and
    thus auxin canonical signaling. To follow the study of rapid growth regulation,
    we analyzed another rapid growth regulator, signaling peptide RALF1. We showed
    that RALF1 also triggers a rapid and reversible growth inhibition caused by H
    + influx, highly resembling but not dependent on auxin. Besides, RALF1 promotes
    auxin biosynthesis by increasing expression of auxin biosynthesis enzyme YUCCAs
    and thus induces auxin signaling in ca. 1 hour, contributing to the sustained
    RALF1-triggered growth inhibition. These studies collectively contribute to understanding
    rapid regulation on plant cell\r\ngrowth, novel auxin signaling pathway as well
    as auxin-peptide crosstalk. @eng"
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Lanxin
      foaf_name: Li, Lanxin
      foaf_surname: Li
      foaf_workInfoHomepage: http://www.librecat.org/personId=367EF8FA-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0002-5607-272X
  bibo_doi: 10.15479/at:ista:10083
  dct_date: 2021^xs_gYear
  dct_isPartOf:
  - http://id.crossref.org/issn/2663-337X
  dct_language: eng
  dct_publisher: Institute of Science and Technology Austria@
  dct_title: Rapid cell growth regulation in Arabidopsis@
...