---
res:
  bibo_abstract:
  - Proximity labeling provides a powerful in vivo tool to characterize the proteome
    of subcellular structures and the interactome of specific proteins. The nematode
    Caenorhabditis elegans is one of the most intensely studied organisms in biology,
    offering many advantages for biochemistry. Using the highly active biotin ligase
    TurboID, we optimize here a proximity labeling protocol for C. elegans. An advantage
    of TurboID is that biotin's high affinity for streptavidin means biotin-labeled
    proteins can be affinity-purified under harsh denaturing conditions. By combining
    extensive sonication with aggressive denaturation using SDS and urea, we achieved
    near-complete solubilization of worm proteins. We then used this protocol to characterize
    the proteomes of the worm gut, muscle, skin, and nervous system. Neurons are among
    the smallest C. elegans cells. To probe the method's sensitivity, we expressed
    TurboID exclusively in the two AFD neurons and showed that the protocol could
    identify known and previously unknown proteins expressed selectively in AFD. The
    active zones of synapses are composed of a protein matrix that is difficult to
    solubilize and purify. To test if our protocol could solubilize active zone proteins,
    we knocked TurboID into the endogenous elks-1 gene, which encodes a presynaptic
    active zone protein. We identified many known ELKS-1-interacting active zone proteins,
    as well as previously uncharacterized synaptic proteins. Versatile vectors and
    the inherent advantages of using C. elegans, including fast growth and the ability
    to rapidly make and functionally test knock-ins, make proximity labeling a valuable
    addition to the armory of this model organism.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Murat
      foaf_name: Artan, Murat
      foaf_surname: Artan
      foaf_workInfoHomepage: http://www.librecat.org/personId=C407B586-6052-11E9-B3AE-7006E6697425
    orcid: 0000-0001-8945-6992
  - foaf_Person:
      foaf_givenName: Stephen
      foaf_name: Barratt, Stephen
      foaf_surname: Barratt
      foaf_workInfoHomepage: http://www.librecat.org/personId=57740d2b-2a88-11ec-97cf-d9e6d1b39677
  - foaf_Person:
      foaf_givenName: Sean M.
      foaf_name: Flynn, Sean M.
      foaf_surname: Flynn
  - foaf_Person:
      foaf_givenName: Farida
      foaf_name: Begum, Farida
      foaf_surname: Begum
  - foaf_Person:
      foaf_givenName: Mark
      foaf_name: Skehel, Mark
      foaf_surname: Skehel
  - foaf_Person:
      foaf_givenName: Armel
      foaf_name: Nicolas, Armel
      foaf_surname: Nicolas
      foaf_workInfoHomepage: http://www.librecat.org/personId=2A103192-F248-11E8-B48F-1D18A9856A87
  - foaf_Person:
      foaf_givenName: Mario
      foaf_name: De Bono, Mario
      foaf_surname: De Bono
      foaf_workInfoHomepage: http://www.librecat.org/personId=4E3FF80E-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0001-8347-0443
  bibo_doi: 10.1016/J.JBC.2021.101094
  bibo_issue: '3'
  bibo_volume: 297
  dct_date: 2021^xs_gYear
  dct_identifier:
  - UT:000706409200006
  dct_isPartOf:
  - http://id.crossref.org/issn/0021-9258
  - http://id.crossref.org/issn/1083-351X
  dct_language: eng
  dct_publisher: Elsevier@
  dct_title: Interactome analysis of Caenorhabditis elegans synapses by TurboID-based
    proximity labeling@
...