--- res: bibo_abstract: - The analysis of dynamic cellular processes such as plant cytokinesis stands and falls with live-cell time-lapse confocal imaging. Conventional approaches to time-lapse imaging of cell division in Arabidopsis root tips are tedious and have low throughput. Here, we describe a protocol for long-term time-lapse simultaneous imaging of multiple root tips on a vertical-stage confocal microscope with automated root tracking. We also provide modifications of the basic protocol to implement this imaging method in the analysis of genetic, pharmacological or laser ablation wounding-mediated experimental manipulations. Our method dramatically improves the efficiency of cell division time-lapse imaging by increasing the throughput, while reducing the person-hour requirements of such experiments.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Lukas foaf_name: Hörmayer, Lukas foaf_surname: Hörmayer foaf_workInfoHomepage: http://www.librecat.org/personId=2EEE7A2A-F248-11E8-B48F-1D18A9856A87 - foaf_Person: foaf_givenName: Jiří foaf_name: Friml, Jiří foaf_surname: Friml foaf_workInfoHomepage: http://www.librecat.org/personId=4159519E-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-8302-7596 - foaf_Person: foaf_givenName: Matous foaf_name: Glanc, Matous foaf_surname: Glanc foaf_workInfoHomepage: http://www.librecat.org/personId=1AE1EA24-02D0-11E9-9BAA-DAF4881429F2 orcid: 0000-0003-0619-7783 bibo_doi: 10.1007/978-1-0716-1744-1_6 bibo_volume: 2382 dct_date: 2021^xs_gYear dct_isPartOf: - http://id.crossref.org/issn/1064-3745 - http://id.crossref.org/issn/1940-6029 - http://id.crossref.org/issn/978-1-0716-1743-4 dct_language: eng dct_publisher: Humana Press@ dct_title: Automated time-lapse imaging and manipulation of cell divisions in Arabidopsis roots by vertical-stage confocal microscopy@ fabio_hasPubmedId: '34705235' ...