---
_id: '10316'
abstract:
- lang: eng
  text: A key attribute of persistent or recurring bacterial infections is the ability
    of the pathogen to evade the host’s immune response. Many Enterobacteriaceae express
    type 1 pili, a pre-adapted virulence trait, to invade host epithelial cells and
    establish persistent infections. However, the molecular mechanisms and strategies
    by which bacteria actively circumvent the immune response of the host remain poorly
    understood. Here, we identified CD14, the major co-receptor for lipopolysaccharide
    detection, on dendritic cells as a previously undescribed binding partner of FimH,
    the protein located at the tip of the type 1 pilus of Escherichia coli. The FimH
    amino acids involved in CD14 binding are highly conserved across pathogenic and
    non-pathogenic strains. Binding of pathogenic bacteria to CD14 lead to reduced
    dendritic cell migration and blunted expression of co-stimulatory molecules, both
    rate-limiting factors of T cell activation. While defining an active molecular
    mechanism of immune evasion by pathogens, the interaction between FimH and CD14
    represents a potential target to interfere with persistent and recurrent infections,
    such as urinary tract infections or Crohn’s disease.
acknowledged_ssus:
- _id: Bio
- _id: PreCl
- _id: EM-Fac
acknowledgement: We thank Ulrich Dobrindt for providing UPEC strain CFT073, Vlad Gavra
  and Maximilian Götz, Bor Kavčič, Jonna Alanko and Eva Kiermaier for help with experiments
  and Robert Hauschild, Julian Stopp and Saren Tasciyan for help with data analysis.
  We thank the IST Austria Scientific Service Units, especially the Bioimaging facility,
  the Preclinical facility and the Electron microscopy facility for technical support,
  Jakob Wallner and all members of the Guet and Sixt lab for fruitful discussions
  and Daria Siekhaus for critically reading the manuscript. This work was supported
  by grants from the Austrian Research Promotion Agency (FEMtech 868984) to I.G.,
  the European Research Council (CoG 724373) and the Austrian Science Fund (FWF P29911)
  to M.S.
article_processing_charge: No
author:
- first_name: Kathrin
  full_name: Tomasek, Kathrin
  id: 3AEC8556-F248-11E8-B48F-1D18A9856A87
  last_name: Tomasek
  orcid: 0000-0003-3768-877X
- first_name: Alexander F
  full_name: Leithner, Alexander F
  id: 3B1B77E4-F248-11E8-B48F-1D18A9856A87
  last_name: Leithner
  orcid: 0000-0002-1073-744X
- first_name: Ivana
  full_name: Glatzová, Ivana
  id: 727b3c7d-4939-11ec-89b3-b9b0750ab74d
  last_name: Glatzová
- first_name: Michael S.
  full_name: Lukesch, Michael S.
  last_name: Lukesch
- first_name: Calin C
  full_name: Guet, Calin C
  id: 47F8433E-F248-11E8-B48F-1D18A9856A87
  last_name: Guet
  orcid: 0000-0001-6220-2052
- first_name: Michael K
  full_name: Sixt, Michael K
  id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
  last_name: Sixt
  orcid: 0000-0002-4561-241X
citation:
  ama: Tomasek K, Leithner AF, Glatzová I, Lukesch MS, Guet CC, Sixt MK. Type 1 piliated
    uropathogenic Escherichia coli hijack the host immune response by binding to CD14.
    <i>bioRxiv</i>. doi:<a href="https://doi.org/10.1101/2021.10.18.464770">10.1101/2021.10.18.464770</a>
  apa: Tomasek, K., Leithner, A. F., Glatzová, I., Lukesch, M. S., Guet, C. C., &#38;
    Sixt, M. K. (n.d.). Type 1 piliated uropathogenic Escherichia coli hijack the
    host immune response by binding to CD14. <i>bioRxiv</i>. <a href="https://doi.org/10.1101/2021.10.18.464770">https://doi.org/10.1101/2021.10.18.464770</a>
  chicago: Tomasek, Kathrin, Alexander F Leithner, Ivana Glatzová, Michael S. Lukesch,
    Calin C Guet, and Michael K Sixt. “Type 1 Piliated Uropathogenic Escherichia Coli
    Hijack the Host Immune Response by Binding to CD14.” <i>BioRxiv</i>, n.d. <a href="https://doi.org/10.1101/2021.10.18.464770">https://doi.org/10.1101/2021.10.18.464770</a>.
  ieee: K. Tomasek, A. F. Leithner, I. Glatzová, M. S. Lukesch, C. C. Guet, and M.
    K. Sixt, “Type 1 piliated uropathogenic Escherichia coli hijack the host immune
    response by binding to CD14,” <i>bioRxiv</i>. .
  ista: Tomasek K, Leithner AF, Glatzová I, Lukesch MS, Guet CC, Sixt MK. Type 1 piliated
    uropathogenic Escherichia coli hijack the host immune response by binding to CD14.
    bioRxiv, <a href="https://doi.org/10.1101/2021.10.18.464770">10.1101/2021.10.18.464770</a>.
  mla: Tomasek, Kathrin, et al. “Type 1 Piliated Uropathogenic Escherichia Coli Hijack
    the Host Immune Response by Binding to CD14.” <i>BioRxiv</i>, doi:<a href="https://doi.org/10.1101/2021.10.18.464770">10.1101/2021.10.18.464770</a>.
  short: K. Tomasek, A.F. Leithner, I. Glatzová, M.S. Lukesch, C.C. Guet, M.K. Sixt,
    BioRxiv (n.d.).
corr_author: '1'
das_tickbox: '1'
date_created: 2021-11-19T12:24:16Z
date_published: 2021-10-18T00:00:00Z
date_updated: 2026-07-14T22:31:12Z
day: '18'
department:
- _id: CaGu
- _id: MiSi
doi: 10.1101/2021.10.18.464770
ec_funded: 1
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://www.biorxiv.org/content/10.1101/2021.10.18.464770v1
month: '10'
oa: 1
oa_version: Preprint
project:
- _id: 25FE9508-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '724373'
  name: Cellular Navigation Along Spatial Gradients
- _id: 26018E70-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: P29911
  name: Mechanical adaptation of lamellipodial actin
publication: bioRxiv
publication_status: draft
related_material:
  record:
  - id: '11843'
    relation: later_version
    status: public
  - id: '10307'
    relation: dissertation_contains
    status: public
status: public
title: Type 1 piliated uropathogenic Escherichia coli hijack the host immune response
  by binding to CD14
type: preprint
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2021'
...