---
_id: '10321'
abstract:
- lang: eng
  text: Mosaic analysis with double markers (MADM) technology enables the generation
    of genetic mosaic tissue in mice. MADM enables concomitant fluorescent cell labeling
    and introduction of a mutation of a gene of interest with single-cell resolution.
    This protocol highlights major steps for the generation of genetic mosaic tissue
    and the isolation and processing of respective tissues for downstream histological
    analysis. For complete details on the use and execution of this protocol, please
    refer to Contreras et al. (2021).
acknowledged_ssus:
- _id: Bio
- _id: PreCl
acknowledgement: This research was supported by the Scientific Service Units (SSU)
  at IST Austria through resources provided by the Bioimaging (BIF) and Preclinical
  Facilities (PCF). We particularly thank Mohammad Goudarzi for assistance with photography
  of mouse perfusion and dissection. N.A. received support from FWF Firnberg-Programm
  (T 1031). This work was also supported by IST Austria institutional funds; FWF SFB
  F78 to S.H.; and the European Research Council (ERC) under the European Union’s
  Horizon 2020 research and innovation programme (grant agreement no. 725780 LinPro)
  to S.H.
article_number: '100939'
article_processing_charge: Yes
article_type: original
author:
- first_name: Nicole
  full_name: Amberg, Nicole
  id: 4CD6AAC6-F248-11E8-B48F-1D18A9856A87
  last_name: Amberg
  orcid: 0000-0002-3183-8207
- first_name: Simon
  full_name: Hippenmeyer, Simon
  id: 37B36620-F248-11E8-B48F-1D18A9856A87
  last_name: Hippenmeyer
  orcid: 0000-0003-2279-1061
citation:
  ama: Amberg N, Hippenmeyer S. Genetic mosaic dissection of candidate genes in mice
    using mosaic analysis with double markers. <i>STAR Protocols</i>. 2021;2(4). doi:<a
    href="https://doi.org/10.1016/j.xpro.2021.100939">10.1016/j.xpro.2021.100939</a>
  apa: Amberg, N., &#38; Hippenmeyer, S. (2021). Genetic mosaic dissection of candidate
    genes in mice using mosaic analysis with double markers. <i>STAR Protocols</i>.
    Cell Press. <a href="https://doi.org/10.1016/j.xpro.2021.100939">https://doi.org/10.1016/j.xpro.2021.100939</a>
  chicago: Amberg, Nicole, and Simon Hippenmeyer. “Genetic Mosaic Dissection of Candidate
    Genes in Mice Using Mosaic Analysis with Double Markers.” <i>STAR Protocols</i>.
    Cell Press, 2021. <a href="https://doi.org/10.1016/j.xpro.2021.100939">https://doi.org/10.1016/j.xpro.2021.100939</a>.
  ieee: N. Amberg and S. Hippenmeyer, “Genetic mosaic dissection of candidate genes
    in mice using mosaic analysis with double markers,” <i>STAR Protocols</i>, vol.
    2, no. 4. Cell Press, 2021.
  ista: Amberg N, Hippenmeyer S. 2021. Genetic mosaic dissection of candidate genes
    in mice using mosaic analysis with double markers. STAR Protocols. 2(4), 100939.
  mla: Amberg, Nicole, and Simon Hippenmeyer. “Genetic Mosaic Dissection of Candidate
    Genes in Mice Using Mosaic Analysis with Double Markers.” <i>STAR Protocols</i>,
    vol. 2, no. 4, 100939, Cell Press, 2021, doi:<a href="https://doi.org/10.1016/j.xpro.2021.100939">10.1016/j.xpro.2021.100939</a>.
  short: N. Amberg, S. Hippenmeyer, STAR Protocols 2 (2021).
corr_author: '1'
date_created: 2021-11-21T23:01:28Z
date_published: 2021-11-10T00:00:00Z
date_updated: 2025-04-15T08:23:07Z
day: '10'
ddc:
- '573'
department:
- _id: SiHi
doi: 10.1016/j.xpro.2021.100939
ec_funded: 1
file:
- access_level: open_access
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  content_type: application/pdf
  creator: cchlebak
  date_created: 2021-11-22T08:23:58Z
  date_updated: 2021-11-22T08:23:58Z
  file_id: '10329'
  file_name: 2021_STARProtocols_Amberg.pdf
  file_size: 7309464
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file_date_updated: 2021-11-22T08:23:58Z
has_accepted_license: '1'
intvolume: '         2'
issue: '4'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '11'
oa: 1
oa_version: Published Version
project:
- _id: 260018B0-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '725780'
  name: Principles of Neural Stem Cell Lineage Progression in Cerebral Cortex Development
- _id: 268F8446-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: T01031
  name: Role of Eed in neural stem cell lineage progression
- _id: 059F6AB4-7A3F-11EA-A408-12923DDC885E
  grant_number: F7805
  name: Stem Cell Modulation in Neural Development and Regeneration/ P05-Molecular
    Mechanisms of Neural Stem Cell Lineage Progression
publication: STAR Protocols
publication_identifier:
  eissn:
  - 2666-1667
publication_status: published
publisher: Cell Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: Genetic mosaic dissection of candidate genes in mice using mosaic analysis
  with double markers
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 2
year: '2021'
...