Catabolism of strigolactones by a carboxylesterase

article Catabolism of strigolactones by a carboxylesterase published yes Enjun Xu author Liang Chai author Shiqi Zhang author Ruixue Yu author Xixi Zhang author 61A66458-47E9-11EA-85BA-8AEAAF14E49A0000-0001-7048-4627 Chongyi Xu author Yuxin Hu author JiFr department Strigolactones (SLs) are carotenoid-derived plant hormones that control shoot branching and communications between host plants and symbiotic fungi or root parasitic plants. Extensive studies have identified the key components participating in SL biosynthesis and signalling, whereas the catabolism or deactivation of endogenous SLs in planta remains largely unknown. Here, we report that the Arabidopsis carboxylesterase 15 (AtCXE15) and its orthologues function as efficient hydrolases of SLs. We show that overexpression of AtCXE15 promotes shoot branching by dampening SL-inhibited axillary bud outgrowth. We further demonstrate that AtCXE15 could bind and efficiently hydrolyse SLs both in vitro and in planta. We also provide evidence that AtCXE15 is capable of catalysing hydrolysis of diverse SL analogues and that such CXE15-dependent catabolism of SLs is evolutionarily conserved in seed plants. These results disclose a catalytic mechanism underlying homoeostatic regulation of SLs in plants, which also provides a rational approach to spatial-temporally manipulate the endogenous SLs and thus architecture of crops and ornamental plants. https://research-explorer.ista.ac.at/download/10326/18864/Accepted version_Xu et al.,2021 Catabolism of strigolactones by a carboxylesterase.pdf application/pdfno Springer Nature2021 eng Nature Plants 2055-0278 34764442 00071740800000210.1038/s41477-021-01011-y 71495–1504 Xu E, Chai L, Zhang S, Yu R, Zhang X, Xu C, Hu Y. 2021. Catabolism of strigolactones by a carboxylesterase. Nature Plants. 7, 1495–1504. Xu E, Chai L, Zhang S, et al. Catabolism of strigolactones by a carboxylesterase. Nature Plants. 2021;7:1495–1504. doi:<a href="https://doi.org/10.1038/s41477-021-01011-y">10.1038/s41477-021-01011-y</a> Xu, E., Chai, L., Zhang, S., Yu, R., Zhang, X., Xu, C., & Hu, Y. (2021). Catabolism of strigolactones by a carboxylesterase. Nature Plants. Springer Nature. <a href="https://doi.org/10.1038/s41477-021-01011-y">https://doi.org/10.1038/s41477-021-01011-y</a> E. Xu et al., “Catabolism of strigolactones by a carboxylesterase,” Nature Plants, vol. 7. Springer Nature, pp. 1495–1504, 2021. E. Xu, L. Chai, S. Zhang, R. Yu, X. Zhang, C. Xu, Y. Hu, Nature Plants 7 (2021) 1495–1504. Xu, Enjun, Liang Chai, Shiqi Zhang, Ruixue Yu, Xixi Zhang, Chongyi Xu, and Yuxin Hu. “Catabolism of Strigolactones by a Carboxylesterase.” Nature Plants. Springer Nature, 2021. <a href="https://doi.org/10.1038/s41477-021-01011-y">https://doi.org/10.1038/s41477-021-01011-y</a>. Xu, Enjun, et al. “Catabolism of Strigolactones by a Carboxylesterase.” Nature Plants, vol. 7, Springer Nature, 2021, pp. 1495–1504, doi:<a href="https://doi.org/10.1038/s41477-021-01011-y">10.1038/s41477-021-01011-y</a>. 103262021-11-21T23:01:30Z2025-01-21T12:42:52Z