---
_id: '10349'
abstract:
- lang: eng
  text: Sulfolobus acidocaldarius is the closest experimentally tractable archaeal
    relative of eukaryotes and, despite lacking obvious cyclin-dependent kinase and
    cyclin homologs, has an ordered eukaryote-like cell cycle with distinct phases
    of DNA replication and division. Here, in exploring the mechanism of cell division
    in S. acidocaldarius, we identify a role for the archaeal proteasome in regulating
    the transition from the end of one cell cycle to the beginning of the next. Further,
    we identify the archaeal ESCRT-III homolog, CdvB, as a key target of the proteasome
    and show that its degradation triggers division by allowing constriction of the
    CdvB1:CdvB2 ESCRT-III division ring. These findings offer a minimal mechanism
    for ESCRT-III–mediated membrane remodeling and point to a conserved role for the
    proteasome in eukaryotic and archaeal cell cycle control.
acknowledgement: "We thank the MRC LMCB at UCL for their support; the flow cytometry
  STP at the Francis Crick Institute for assistance, with special thanks to S. Purewal
  and D. Davis; C. Bertoli for mentorship\r\nand advice; J. M. Garcia-Arcos for help
  early on in this project; the entire Baum lab for their input throughout the project;
  the Albers lab for advice and reagents, with special thanks to M. Van Wolferen and
  S. Albers; the members of the Wellcome consortium for archaeal cytoskeleton studies
  for advice and comments; and J. Löwe, S. Oliferenko, M. Balasubramanian, and D.
  Gerlich for discussions and advice on the manuscript. N.P.R. and S.B. would like
  to thank N. Rzechorzek, A. Simon, and S. Anjum for discussion and advice."
article_processing_charge: No
article_type: original
author:
- first_name: Gabriel
  full_name: Tarrason Risa, Gabriel
  last_name: Tarrason Risa
- first_name: Fredrik
  full_name: Hurtig, Fredrik
  last_name: Hurtig
- first_name: Sian
  full_name: Bray, Sian
  last_name: Bray
- first_name: Anne E.
  full_name: Hafner, Anne E.
  last_name: Hafner
- first_name: Lena
  full_name: Harker-Kirschneck, Lena
  last_name: Harker-Kirschneck
- first_name: Peter
  full_name: Faull, Peter
  last_name: Faull
- first_name: Colin
  full_name: Davis, Colin
  last_name: Davis
- first_name: Dimitra
  full_name: Papatziamou, Dimitra
  last_name: Papatziamou
- first_name: Delyan R.
  full_name: Mutavchiev, Delyan R.
  last_name: Mutavchiev
- first_name: Catherine
  full_name: Fan, Catherine
  last_name: Fan
- first_name: Leticia
  full_name: Meneguello, Leticia
  last_name: Meneguello
- first_name: Andre
  full_name: Arashiro Pulschen, Andre
  last_name: Arashiro Pulschen
- first_name: Gautam
  full_name: Dey, Gautam
  last_name: Dey
- first_name: Siân
  full_name: Culley, Siân
  last_name: Culley
- first_name: Mairi
  full_name: Kilkenny, Mairi
  last_name: Kilkenny
- first_name: Diorge P.
  full_name: Souza, Diorge P.
  last_name: Souza
- first_name: Luca
  full_name: Pellegrini, Luca
  last_name: Pellegrini
- first_name: Robertus A. M.
  full_name: de Bruin, Robertus A. M.
  last_name: de Bruin
- first_name: Ricardo
  full_name: Henriques, Ricardo
  last_name: Henriques
- first_name: Ambrosius P.
  full_name: Snijders, Ambrosius P.
  last_name: Snijders
- first_name: Anđela
  full_name: Šarić, Anđela
  id: bf63d406-f056-11eb-b41d-f263a6566d8b
  last_name: Šarić
  orcid: 0000-0002-7854-2139
- first_name: Ann-Christin
  full_name: Lindås, Ann-Christin
  last_name: Lindås
- first_name: Nicholas P.
  full_name: Robinson, Nicholas P.
  last_name: Robinson
- first_name: Buzz
  full_name: Baum, Buzz
  last_name: Baum
citation:
  ama: Tarrason Risa G, Hurtig F, Bray S, et al. The proteasome controls ESCRT-III–mediated
    cell division in an archaeon. <i>Science</i>. 2020;369(6504). doi:<a href="https://doi.org/10.1126/science.aaz2532">10.1126/science.aaz2532</a>
  apa: Tarrason Risa, G., Hurtig, F., Bray, S., Hafner, A. E., Harker-Kirschneck,
    L., Faull, P., … Baum, B. (2020). The proteasome controls ESCRT-III–mediated cell
    division in an archaeon. <i>Science</i>. American Association for the Advancement
    of Science. <a href="https://doi.org/10.1126/science.aaz2532">https://doi.org/10.1126/science.aaz2532</a>
  chicago: Tarrason Risa, Gabriel, Fredrik Hurtig, Sian Bray, Anne E. Hafner, Lena
    Harker-Kirschneck, Peter Faull, Colin Davis, et al. “The Proteasome Controls ESCRT-III–Mediated
    Cell Division in an Archaeon.” <i>Science</i>. American Association for the Advancement
    of Science, 2020. <a href="https://doi.org/10.1126/science.aaz2532">https://doi.org/10.1126/science.aaz2532</a>.
  ieee: G. Tarrason Risa <i>et al.</i>, “The proteasome controls ESCRT-III–mediated
    cell division in an archaeon,” <i>Science</i>, vol. 369, no. 6504. American Association
    for the Advancement of Science, 2020.
  ista: Tarrason Risa G, Hurtig F, Bray S, Hafner AE, Harker-Kirschneck L, Faull P,
    Davis C, Papatziamou D, Mutavchiev DR, Fan C, Meneguello L, Arashiro Pulschen
    A, Dey G, Culley S, Kilkenny M, Souza DP, Pellegrini L, de Bruin RAM, Henriques
    R, Snijders AP, Šarić A, Lindås A-C, Robinson NP, Baum B. 2020. The proteasome
    controls ESCRT-III–mediated cell division in an archaeon. Science. 369(6504).
  mla: Tarrason Risa, Gabriel, et al. “The Proteasome Controls ESCRT-III–Mediated
    Cell Division in an Archaeon.” <i>Science</i>, vol. 369, no. 6504, American Association
    for the Advancement of Science, 2020, doi:<a href="https://doi.org/10.1126/science.aaz2532">10.1126/science.aaz2532</a>.
  short: G. Tarrason Risa, F. Hurtig, S. Bray, A.E. Hafner, L. Harker-Kirschneck,
    P. Faull, C. Davis, D. Papatziamou, D.R. Mutavchiev, C. Fan, L. Meneguello, A.
    Arashiro Pulschen, G. Dey, S. Culley, M. Kilkenny, D.P. Souza, L. Pellegrini,
    R.A.M. de Bruin, R. Henriques, A.P. Snijders, A. Šarić, A.-C. Lindås, N.P. Robinson,
    B. Baum, Science 369 (2020).
date_created: 2021-11-26T08:21:34Z
date_published: 2020-08-07T00:00:00Z
date_updated: 2021-11-26T08:58:33Z
day: '07'
doi: 10.1126/science.aaz2532
extern: '1'
external_id:
  pmid:
  - '32764038'
intvolume: '       369'
issue: '6504'
keyword:
- multidisciplinary
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://www.biorxiv.org/content/10.1101/774273v1
month: '08'
oa: 1
oa_version: Preprint
pmid: 1
publication: Science
publication_identifier:
  eissn:
  - 1095-9203
  issn:
  - 0036-8075
publication_status: published
publisher: American Association for the Advancement of Science
quality_controlled: '1'
scopus_import: '1'
status: public
title: The proteasome controls ESCRT-III–mediated cell division in an archaeon
type: journal_article
user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9
volume: 369
year: '2020'
...