--- _id: '1059' abstract: - lang: eng text: A range of bright and photostable rhodamines and carbopyronines with absorption maxima in the range of λ=500-630 nm were prepared, and enabled the specific labeling of cytoskeletal filaments using HaloTag technology followed by staining with 1 μm solutions of the dye-ligand conjugates. The synthesis, photophysical parameters, fluorogenic behavior, and structure-property relationships of the new dyes are discussed. Light microscopy with stimulated emission depletion (STED) provided one- and two-color images of living cells with an optical resolution of 40-60 nm. acknowledgement: We thank Prof. Y. Okada (RIKEN Quantitative Biology Center, Osaka, Japan) for the gift of β-tubulin-Halo plasmid, T. Gilat and Dr. E. Rothermel (MPIBPC, Göttingen, Germany) for cell culture and transfection, M. Pulst, J. Bienert (MPIBPC), Dr. M. John, Dr. H. Frauendorf, and co-workers (Institut für Organische und Biomolekulare Chemie, Georg-August-Universität, Göttingen, Germany) for UV/Vis, NMR, and ESI-MS spectra, Prof. M. L. Bossi (University of Buenos-Aires, Argentina) for measuring fluorescence lifetimes, and Dr. S. Vos and Prof. P. Cramer (MPIBPC) for access to a Tecan microplate reader. S.W.H. acknowledges a grant from the Bundesministerium für Bildung und Forschung (BMBF 513) within the program “Optische Technologien für Biowissenschaften und Gesundheit” (FKZ 13N11066). J.G.D. was supported by funds from the People Programme (Marie Curie Actions) of the European Union's Seventh Framework Programme (FP7/2007–2013; REA grant agreement PIEF-GA-2011-299283). article_processing_charge: No author: - first_name: Alexey full_name: Butkevich, Alexey last_name: Butkevich - first_name: Gyuzel full_name: Mitronova, Gyuzel last_name: Mitronova - first_name: Sven full_name: Sidenstein, Sven last_name: Sidenstein - first_name: Jessica full_name: Klocke, Jessica last_name: Klocke - first_name: Dirk full_name: Kamin, Dirk last_name: Kamin - first_name: Dirk full_name: Meineke, Dirk last_name: Meineke - first_name: Elisa full_name: D'Este, Elisa last_name: D'Este - first_name: Philip full_name: Kraemer, Philip last_name: Kraemer - first_name: Johann G full_name: Danzl, Johann G id: 42EFD3B6-F248-11E8-B48F-1D18A9856A87 last_name: Danzl orcid: 0000-0001-8559-3973 - first_name: Vladimir full_name: Belov, Vladimir last_name: Belov - first_name: Stefan full_name: Hell, Stefan last_name: Hell citation: ama: Butkevich A, Mitronova G, Sidenstein S, et al. Fluorescent rhodamines and fluorogenic carbopyronines for super-resolution STED microscopy in living cells. Angewandte Chemie - International Edition. 2016;55(10):3290-3294. doi:10.1002/anie.201511018 apa: Butkevich, A., Mitronova, G., Sidenstein, S., Klocke, J., Kamin, D., Meineke, D., … Hell, S. (2016). Fluorescent rhodamines and fluorogenic carbopyronines for super-resolution STED microscopy in living cells. Angewandte Chemie - International Edition. Wiley-Blackwell. https://doi.org/10.1002/anie.201511018 chicago: Butkevich, Alexey, Gyuzel Mitronova, Sven Sidenstein, Jessica Klocke, Dirk Kamin, Dirk Meineke, Elisa D’Este, et al. “Fluorescent Rhodamines and Fluorogenic Carbopyronines for Super-Resolution STED Microscopy in Living Cells.” Angewandte Chemie - International Edition. Wiley-Blackwell, 2016. https://doi.org/10.1002/anie.201511018. ieee: A. Butkevich et al., “Fluorescent rhodamines and fluorogenic carbopyronines for super-resolution STED microscopy in living cells,” Angewandte Chemie - International Edition, vol. 55, no. 10. Wiley-Blackwell, pp. 3290–3294, 2016. ista: Butkevich A, Mitronova G, Sidenstein S, Klocke J, Kamin D, Meineke D, D’Este E, Kraemer P, Danzl JG, Belov V, Hell S. 2016. Fluorescent rhodamines and fluorogenic carbopyronines for super-resolution STED microscopy in living cells. Angewandte Chemie - International Edition. 55(10), 3290–3294. mla: Butkevich, Alexey, et al. “Fluorescent Rhodamines and Fluorogenic Carbopyronines for Super-Resolution STED Microscopy in Living Cells.” Angewandte Chemie - International Edition, vol. 55, no. 10, Wiley-Blackwell, 2016, pp. 3290–94, doi:10.1002/anie.201511018. short: A. Butkevich, G. Mitronova, S. Sidenstein, J. Klocke, D. Kamin, D. Meineke, E. D’Este, P. Kraemer, J.G. Danzl, V. Belov, S. Hell, Angewandte Chemie - International Edition 55 (2016) 3290–3294. date_created: 2018-12-11T11:49:55Z date_published: 2016-03-01T00:00:00Z date_updated: 2021-01-12T06:47:59Z day: '01' doi: 10.1002/anie.201511018 extern: '1' intvolume: ' 55' issue: '10' language: - iso: eng license: https://creativecommons.org/licenses/by-nc-nd/4.0/ month: '03' oa_version: None page: 3290 - 3294 publication: Angewandte Chemie - International Edition publication_status: published publisher: Wiley-Blackwell publist_id: '6330' status: public title: Fluorescent rhodamines and fluorogenic carbopyronines for super-resolution STED microscopy in living cells tmp: image: /images/cc_by_nc_nd.png legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) short: CC BY-NC-ND (4.0) type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 55 year: '2016' ...