---
_id: '10606'
abstract:
- lang: eng
text: Cell division orientation is thought to result from a competition between
cell geometry and polarity domains controlling the position of the mitotic spindle
during mitosis. Depending on the level of cell shape anisotropy or the strength
of the polarity domain, one dominates the other and determines the orientation
of the spindle. Whether and how such competition is also at work to determine
unequal cell division (UCD), producing daughter cells of different size, remains
unclear. Here, we show that cell geometry and polarity domains cooperate, rather
than compete, in positioning the cleavage plane during UCDs in early ascidian
embryos. We found that the UCDs and their orientation at the ascidian third cleavage
rely on the spindle tilting in an anisotropic cell shape, and cortical polarity
domains exerting different effects on spindle astral microtubules. By systematically
varying mitotic cell shape, we could modulate the effect of attractive and repulsive
polarity domains and consequently generate predicted daughter cell size asymmetries
and position. We therefore propose that the spindle position during UCD is set
by the combined activities of cell geometry and polarity domains, where cell geometry
modulates the effect of cortical polarity domain(s).
acknowledged_ssus:
- _id: NanoFab
- _id: Bio
acknowledgement: 'We thank members of the Heisenberg and McDougall groups for technical
advice and discussion. We are grateful to the Bioimaging and Nanofabrication facilities
of IST Austria and the Imaging Platform (PIM) and animal facility (CRB) of Institut
de la Mer de Villefranche (IMEV), which is supported by EMBRC-France, whose French
state funds are managed by the ANR within the Investments of the Future program
under reference ANR-10-INBS-0, for continuous support. This work was supported by
a collaborative grant from the French Government funding agency Agence National
de la Recherche to McDougall (ANR ''MorCell'': ANR-17-CE 13-0028) and the Austrian
Science Fund to Heisenberg (FWF: I 3601-B27).'
article_number: e75639
article_processing_charge: No
article_type: original
author:
- first_name: Benoit G
full_name: Godard, Benoit G
id: 33280250-F248-11E8-B48F-1D18A9856A87
last_name: Godard
- first_name: Remi
full_name: Dumollard, Remi
last_name: Dumollard
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
- first_name: Alex
full_name: Mcdougall, Alex
last_name: Mcdougall
citation:
ama: Godard BG, Dumollard R, Heisenberg C-PJ, Mcdougall A. Combined effect of cell
geometry and polarity domains determines the orientation of unequal division.
eLife. 2021;10. doi:10.7554/eLife.75639
apa: Godard, B. G., Dumollard, R., Heisenberg, C.-P. J., & Mcdougall, A. (2021).
Combined effect of cell geometry and polarity domains determines the orientation
of unequal division. ELife. eLife Sciences Publications. https://doi.org/10.7554/eLife.75639
chicago: Godard, Benoit G, Remi Dumollard, Carl-Philipp J Heisenberg, and Alex Mcdougall.
“Combined Effect of Cell Geometry and Polarity Domains Determines the Orientation
of Unequal Division.” ELife. eLife Sciences Publications, 2021. https://doi.org/10.7554/eLife.75639.
ieee: B. G. Godard, R. Dumollard, C.-P. J. Heisenberg, and A. Mcdougall, “Combined
effect of cell geometry and polarity domains determines the orientation of unequal
division,” eLife, vol. 10. eLife Sciences Publications, 2021.
ista: Godard BG, Dumollard R, Heisenberg C-PJ, Mcdougall A. 2021. Combined effect
of cell geometry and polarity domains determines the orientation of unequal division.
eLife. 10, e75639.
mla: Godard, Benoit G., et al. “Combined Effect of Cell Geometry and Polarity Domains
Determines the Orientation of Unequal Division.” ELife, vol. 10, e75639,
eLife Sciences Publications, 2021, doi:10.7554/eLife.75639.
short: B.G. Godard, R. Dumollard, C.-P.J. Heisenberg, A. Mcdougall, ELife 10 (2021).
date_created: 2022-01-09T23:01:26Z
date_published: 2021-12-21T00:00:00Z
date_updated: 2023-08-17T06:32:44Z
day: '21'
ddc:
- '570'
department:
- _id: CaHe
doi: 10.7554/eLife.75639
external_id:
isi:
- '000733610100001'
file:
- access_level: open_access
checksum: 759c7a873d554c48a6639e6350746ca6
content_type: application/pdf
creator: alisjak
date_created: 2022-01-10T09:40:37Z
date_updated: 2022-01-10T09:40:37Z
file_id: '10611'
file_name: 2021_eLife_Godard.pdf
file_size: 7769934
relation: main_file
success: 1
file_date_updated: 2022-01-10T09:40:37Z
has_accepted_license: '1'
intvolume: ' 10'
isi: 1
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
project:
- _id: 2646861A-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03601
name: Control of embryonic cleavage pattern
publication: eLife
publication_identifier:
eissn:
- 2050-084X
publication_status: published
publisher: eLife Sciences Publications
quality_controlled: '1'
scopus_import: '1'
status: public
title: Combined effect of cell geometry and polarity domains determines the orientation
of unequal division
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 10
year: '2021'
...