---
res:
  bibo_abstract:
  - With more than 80 members worldwide, the Orthobunyavirus genus in the Peribunyaviridae
    family is a large genus of enveloped RNA viruses, many of which are emerging pathogens
    in humans and livestock. How orthobunyaviruses (OBVs) penetrate and infect mammalian
    host cells remains poorly characterized. Here, we investigated the entry mechanisms
    of the OBV Germiston (GERV). Viral particles were visualized by cryo-electron
    microscopy and appeared roughly spherical with an average diameter of 98 nm. Labeling
    of the virus with fluorescent dyes did not adversely affect its infectivity and
    allowed the monitoring of single particles in fixed and live cells. Using this
    approach, we found that endocytic internalization of bound viruses was asynchronous
    and occurred within 30-40 min. The virus entered Rab5a+ early endosomes and, subsequently,
    late endosomal vacuoles containing Rab7a but not LAMP-1. Infectious entry did
    not require proteolytic cleavage, and endosomal acidification was sufficient and
    necessary for viral fusion. Acid-activated penetration began 15-25 min after initiation
    of virus internalization and relied on maturation of early endosomes to late endosomes.
    The optimal pH for viral membrane fusion was slightly below 6.0, and penetration
    was hampered when the potassium influx was abolished. Overall, our study provides
    real-time visualization of GERV entry into host cells and demonstrates the importance
    of late endosomal maturation in facilitating OBV penetration.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Stefan
      foaf_name: Windhaber, Stefan
      foaf_surname: Windhaber
  - foaf_Person:
      foaf_givenName: Qilin
      foaf_name: Xin, Qilin
      foaf_surname: Xin
  - foaf_Person:
      foaf_givenName: Zina M.
      foaf_name: Uckeley, Zina M.
      foaf_surname: Uckeley
  - foaf_Person:
      foaf_givenName: Jana
      foaf_name: Koch, Jana
      foaf_surname: Koch
  - foaf_Person:
      foaf_givenName: Martin
      foaf_name: Obr, Martin
      foaf_surname: Obr
      foaf_workInfoHomepage: http://www.librecat.org/personId=4741CA5A-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0003-1756-6564
  - foaf_Person:
      foaf_givenName: Céline
      foaf_name: Garnier, Céline
      foaf_surname: Garnier
  - foaf_Person:
      foaf_givenName: Catherine
      foaf_name: Luengo-Guyonnot, Catherine
      foaf_surname: Luengo-Guyonnot
  - foaf_Person:
      foaf_givenName: Maëva
      foaf_name: Duboeuf, Maëva
      foaf_surname: Duboeuf
  - foaf_Person:
      foaf_givenName: Florian KM
      foaf_name: Schur, Florian KM
      foaf_surname: Schur
      foaf_workInfoHomepage: http://www.librecat.org/personId=48AD8942-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0003-4790-8078
  - foaf_Person:
      foaf_givenName: Pierre-Yves
      foaf_name: Lozach, Pierre-Yves
      foaf_surname: Lozach
  bibo_doi: 10.1128/jvi.02146-21
  bibo_issue: '5'
  bibo_volume: 96
  dct_date: 2022^xs_gYear
  dct_identifier:
  - UT:000779305000033
  dct_isPartOf:
  - http://id.crossref.org/issn/0022-538X
  - http://id.crossref.org/issn/1098-5514
  dct_language: eng
  dct_publisher: American Society for Microbiology@
  dct_subject:
  - virology
  - insect science
  - immunology
  - microbiology
  dct_title: The Orthobunyavirus Germiston enters host cells from late endosomes@
  fabio_hasPubmedId: '35019710'
...