---
res:
  bibo_abstract:
  - "Adeno-associated viruses (AAVs) are widely used to deliver genetic material in
    vivo to distinct cell types such as neurons or glial cells, allowing for targeted
    manipulation. Transduction of microglia is mostly excluded from this strategy,
    likely due to the cells’ heterogeneous state upon environmental changes, which
    makes AAV design challenging. Here, we established the retina as a model system
    for microglial AAV validation and optimization. First, we show that AAV2/6 transduced
    microglia in both synaptic layers, where layer preference corresponds to the intravitreal
    or subretinal delivery method. Surprisingly, we observed significantly enhanced
    microglial transduction during photoreceptor degeneration. Thus, we modified the
    AAV6 capsid to reduce heparin binding by introducing four point mutations (K531E,
    R576Q, K493S, and K459S), resulting in increased microglial transduction in the
    outer plexiform layer. Finally, to improve microglial-specific transduction, we
    validated a Cre-dependent transgene delivery cassette for use in combination with
    the Cx3cr1CreERT2 mouse line. Together, our results provide a foundation for future
    studies optimizing AAV-mediated microglia transduction and highlight that environmental
    conditions influence microglial transduction efficiency.\r\n@eng"
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Margaret E
      foaf_name: Maes, Margaret E
      foaf_surname: Maes
      foaf_workInfoHomepage: http://www.librecat.org/personId=3838F452-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0001-9642-1085
  - foaf_Person:
      foaf_givenName: Gabriele M.
      foaf_name: Wögenstein, Gabriele M.
      foaf_surname: Wögenstein
  - foaf_Person:
      foaf_givenName: Gloria
      foaf_name: Colombo, Gloria
      foaf_surname: Colombo
      foaf_workInfoHomepage: http://www.librecat.org/personId=3483CF6C-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0001-9434-8902
  - foaf_Person:
      foaf_givenName: Raquel
      foaf_name: Casado Polanco, Raquel
      foaf_surname: Casado Polanco
      foaf_workInfoHomepage: http://www.librecat.org/personId=15240fc1-dbcd-11ea-9d1d-ac5a786425fd
    orcid: 0000-0001-8293-4568
  - foaf_Person:
      foaf_givenName: Sandra
      foaf_name: Siegert, Sandra
      foaf_surname: Siegert
      foaf_workInfoHomepage: http://www.librecat.org/personId=36ACD32E-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0001-8635-0877
  bibo_doi: 10.1016/j.omtm.2021.09.006
  bibo_volume: 23
  dct_date: 2021^xs_gYear
  dct_identifier:
  - UT:000748748500019
  dct_isPartOf:
  - http://id.crossref.org/issn/2329-0501
  dct_language: eng
  dct_publisher: Elsevier@
  dct_title: Optimizing AAV2/6 microglial targeting identified enhanced efficiency
    in the photoreceptor degenerative environment@
...