---
_id: '10703'
abstract:
- lang: eng
  text: 'When crawling through the body, leukocytes often traverse tissues that are
    densely packed with extracellular matrix and other cells, and this raises the
    question: How do leukocytes overcome compressive mechanical loads? Here, we show
    that the actin cortex of leukocytes is mechanoresponsive and that this responsiveness
    requires neither force sensing via the nucleus nor adhesive interactions with
    a substrate. Upon global compression of the cell body as well as local indentation
    of the plasma membrane, Wiskott-Aldrich syndrome protein (WASp) assembles into
    dot-like structures, providing activation platforms for Arp2/3 nucleated actin
    patches. These patches locally push against the external load, which can be obstructing
    collagen fibers or other cells, and thereby create space to facilitate forward
    locomotion. We show in vitro and in vivo that this WASp function is rate limiting
    for ameboid leukocyte migration in dense but not in loose environments and is
    required for trafficking through diverse tissues such as skin and lymph nodes.'
acknowledged_ssus:
- _id: LifeSc
- _id: Bio
- _id: EM-Fac
acknowledgement: We thank N. Darwish-Miranda, F. Leite, F.P. Assen, and A. Eichner
  for advice and help with experiments. We thank J. Renkawitz, E. Kiermaier, A. Juanes
  Garcia, and M. Avellaneda for critical reading of the manuscript. We thank M. Driscoll
  for advice on fluorescent labeling of collagen gels. This research was supported
  by the Scientific Service Units (SSUs) of IST Austria through resources provided
  by Molecular Biology Services/Lab Support Facility (LSF)/Bioimaging Facility/Electron
  Microscopy Facility. This work was funded by grants from the European Research Council
  ( CoG 724373 ) and the Austrian Science Foundation (FWF) to M.S. F.G. received funding
  from the European Union’s Horizon 2020 research and innovation program under the
  Marie Skłodowska-Curie grant agreement no. 747687.
article_processing_charge: No
article_type: original
author:
- first_name: Florian
  full_name: Gaertner, Florian
  last_name: Gaertner
- first_name: Patricia
  full_name: Dos Reis Rodrigues, Patricia
  id: 26E95904-5160-11E9-9C0B-C5B0DC97E90F
  last_name: Dos Reis Rodrigues
  orcid: 0000-0003-1681-508X
- first_name: Ingrid
  full_name: De Vries, Ingrid
  id: 4C7D837E-F248-11E8-B48F-1D18A9856A87
  last_name: De Vries
- first_name: Miroslav
  full_name: Hons, Miroslav
  id: 4167FE56-F248-11E8-B48F-1D18A9856A87
  last_name: Hons
  orcid: 0000-0002-6625-3348
- first_name: Juan
  full_name: Aguilera, Juan
  last_name: Aguilera
- first_name: Michael
  full_name: Riedl, Michael
  id: 3BE60946-F248-11E8-B48F-1D18A9856A87
  last_name: Riedl
  orcid: 0000-0003-4844-6311
- first_name: Alexander F
  full_name: Leithner, Alexander F
  id: 3B1B77E4-F248-11E8-B48F-1D18A9856A87
  last_name: Leithner
  orcid: 0000-0002-1073-744X
- first_name: Saren
  full_name: Tasciyan, Saren
  id: 4323B49C-F248-11E8-B48F-1D18A9856A87
  last_name: Tasciyan
  orcid: 0000-0003-1671-393X
- first_name: Aglaja
  full_name: Kopf, Aglaja
  id: 31DAC7B6-F248-11E8-B48F-1D18A9856A87
  last_name: Kopf
  orcid: 0000-0002-2187-6656
- first_name: Jack
  full_name: Merrin, Jack
  id: 4515C308-F248-11E8-B48F-1D18A9856A87
  last_name: Merrin
  orcid: 0000-0001-5145-4609
- first_name: Vanessa
  full_name: Zheden, Vanessa
  id: 39C5A68A-F248-11E8-B48F-1D18A9856A87
  last_name: Zheden
  orcid: 0000-0002-9438-4783
- first_name: Walter
  full_name: Kaufmann, Walter
  id: 3F99E422-F248-11E8-B48F-1D18A9856A87
  last_name: Kaufmann
  orcid: 0000-0001-9735-5315
- first_name: Robert
  full_name: Hauschild, Robert
  id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
  last_name: Hauschild
  orcid: 0000-0001-9843-3522
- first_name: Michael K
  full_name: Sixt, Michael K
  id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
  last_name: Sixt
  orcid: 0000-0002-6620-9179
citation:
  ama: Gaertner F, Dos Reis Rodrigues P, de Vries I, et al. WASp triggers mechanosensitive
    actin patches to facilitate immune cell migration in dense tissues. <i>Developmental
    Cell</i>. 2022;57(1):47-62.e9. doi:<a href="https://doi.org/10.1016/j.devcel.2021.11.024">10.1016/j.devcel.2021.11.024</a>
  apa: Gaertner, F., Dos Reis Rodrigues, P., de Vries, I., Hons, M., Aguilera, J.,
    Riedl, M., … Sixt, M. K. (2022). WASp triggers mechanosensitive actin patches
    to facilitate immune cell migration in dense tissues. <i>Developmental Cell</i>.
    Cell Press. <a href="https://doi.org/10.1016/j.devcel.2021.11.024">https://doi.org/10.1016/j.devcel.2021.11.024</a>
  chicago: Gaertner, Florian, Patricia Dos Reis Rodrigues, Ingrid de Vries, Miroslav
    Hons, Juan Aguilera, Michael Riedl, Alexander F Leithner, et al. “WASp Triggers
    Mechanosensitive Actin Patches to Facilitate Immune Cell Migration in Dense Tissues.”
    <i>Developmental Cell</i>. Cell Press, 2022. <a href="https://doi.org/10.1016/j.devcel.2021.11.024">https://doi.org/10.1016/j.devcel.2021.11.024</a>.
  ieee: F. Gaertner <i>et al.</i>, “WASp triggers mechanosensitive actin patches to
    facilitate immune cell migration in dense tissues,” <i>Developmental Cell</i>,
    vol. 57, no. 1. Cell Press, p. 47–62.e9, 2022.
  ista: Gaertner F, Dos Reis Rodrigues P, de Vries I, Hons M, Aguilera J, Riedl M,
    Leithner AF, Tasciyan S, Kopf A, Merrin J, Zheden V, Kaufmann W, Hauschild R,
    Sixt MK. 2022. WASp triggers mechanosensitive actin patches to facilitate immune
    cell migration in dense tissues. Developmental Cell. 57(1), 47–62.e9.
  mla: Gaertner, Florian, et al. “WASp Triggers Mechanosensitive Actin Patches to
    Facilitate Immune Cell Migration in Dense Tissues.” <i>Developmental Cell</i>,
    vol. 57, no. 1, Cell Press, 2022, p. 47–62.e9, doi:<a href="https://doi.org/10.1016/j.devcel.2021.11.024">10.1016/j.devcel.2021.11.024</a>.
  short: F. Gaertner, P. Dos Reis Rodrigues, I. de Vries, M. Hons, J. Aguilera, M.
    Riedl, A.F. Leithner, S. Tasciyan, A. Kopf, J. Merrin, V. Zheden, W. Kaufmann,
    R. Hauschild, M.K. Sixt, Developmental Cell 57 (2022) 47–62.e9.
corr_author: '1'
date_created: 2022-01-30T23:01:33Z
date_published: 2022-01-10T00:00:00Z
date_updated: 2026-03-16T23:30:23Z
day: '10'
ddc:
- '570'
department:
- _id: MiSi
- _id: EM-Fac
- _id: NanoFab
- _id: BjHo
doi: 10.1016/j.devcel.2021.11.024
ec_funded: 1
external_id:
  isi:
  - '000768933800005'
  pmid:
  - '34919802'
intvolume: '        57'
isi: 1
issue: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://www.sciencedirect.com/science/article/pii/S1534580721009497
month: '01'
oa: 1
oa_version: Published Version
page: 47-62.e9
pmid: 1
project:
- _id: 260AA4E2-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '747687'
  name: Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells
- _id: 25FE9508-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '724373'
  name: Cellular Navigation Along Spatial Gradients
publication: Developmental Cell
publication_identifier:
  eissn:
  - 1878-1551
  issn:
  - 1534-5807
publication_status: published
publisher: Cell Press
quality_controlled: '1'
related_material:
  record:
  - id: '12726'
    relation: dissertation_contains
    status: public
  - id: '14530'
    relation: dissertation_contains
    status: public
  - id: '20149'
    relation: dissertation_contains
    status: public
  - id: '12401'
    relation: dissertation_contains
    status: public
scopus_import: '1'
status: public
title: WASp triggers mechanosensitive actin patches to facilitate immune cell migration
  in dense tissues
tmp:
  image: /images/cc_by_nc_nd.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
    (CC BY-NC-ND 4.0)
  short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9
volume: 57
year: '2022'
...