---
_id: '10717'
abstract:
- lang: eng
  text: Much of what we know about the role of auxin in plant development derives
    from exogenous manipulations of auxin distribution and signaling, using inhibitors,
    auxins and auxin analogs. In this context, synthetic auxin analogs, such as 1-Naphtalene
    Acetic Acid (1-NAA), are often favored over the endogenous auxin indole-3-acetic
    acid (IAA), in part due to their higher stability. While such auxin analogs have
    proven to be instrumental to reveal the various faces of auxin, they display in
    some cases distinct bioactivities compared to IAA. Here, we focused on the effect
    of auxin analogs on the accumulation of PIN proteins in Brefeldin A-sensitive
    endosomal aggregations (BFA bodies), and the correlation with the ability to elicit
    Ca 2+ responses. For a set of commonly used auxin analogs, we evaluated if auxin-analog
    induced Ca 2+ signaling inhibits PIN accumulation. Not all auxin analogs elicited
    a Ca 2+ response, and their differential ability to elicit Ca 2+ responses correlated
    partially with their ability to inhibit BFA-body formation. However, in tir1/afb
    and cngc14, 1-NAA-induced Ca 2+ signaling was strongly impaired, yet 1-NAA still
    could inhibit PIN accumulation in BFA bodies. This demonstrates that TIR1/AFB-CNGC14-dependent
    Ca 2+ signaling does not inhibit BFA body formation in Arabidopsis roots.
acknowledgement: "We thank Joerg Kudla (WWU Munster, Germany), Petra Dietrich (F.A.
  University of Erlangen-Nurnberg, Germany) for sharing published materials, and NASC
  for providing seeds. We thank Veronique Storme for help with the statistical analyses.
  Part of the imaging analysis was carried out at NOLIMITS, an advanced imaging facility
  established by the University of Milan.\r\nThis work was supported by grants of
  the China Scholarship Council (CSC) to RW and JC; Fonds Wetenschappelijk Onderzoek
  (FWO) to TB and (G002220N) SV; the special research fund of Ghent University to
  EH; the Deutsche Forschungsgemeinschaft (DFG) through Grants within FOR964 (MK and
  KS); Piano di Sviluppo di Ateneo 2019 (University of Milan) to AC; the European
  Research Council (ERC) T-Rex project 682436 to DVD; the ERC ETAP project 742985
  to JF, and by a PhD fellowship from the University of Milan to MG."
article_number: erac019
article_processing_charge: No
article_type: original
author:
- first_name: R
  full_name: Wang, R
  last_name: Wang
- first_name: E
  full_name: Himschoot, E
  last_name: Himschoot
- first_name: M
  full_name: Grenzi, M
  last_name: Grenzi
- first_name: J
  full_name: Chen, J
  last_name: Chen
- first_name: A
  full_name: Safi, A
  last_name: Safi
- first_name: M
  full_name: Krebs, M
  last_name: Krebs
- first_name: K
  full_name: Schumacher, K
  last_name: Schumacher
- first_name: MK
  full_name: Nowack, MK
  last_name: Nowack
- first_name: W
  full_name: Moeder, W
  last_name: Moeder
- first_name: K
  full_name: Yoshioka, K
  last_name: Yoshioka
- first_name: D
  full_name: Van Damme, D
  last_name: Van Damme
- first_name: I
  full_name: De Smet, I
  last_name: De Smet
- first_name: D
  full_name: Geelen, D
  last_name: Geelen
- first_name: T
  full_name: Beeckman, T
  last_name: Beeckman
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
- first_name: A
  full_name: Costa, A
  last_name: Costa
- first_name: S
  full_name: Vanneste, S
  last_name: Vanneste
citation:
  ama: Wang R, Himschoot E, Grenzi M, et al. Auxin analog-induced Ca2+ signaling is
    independent of inhibition of endosomal aggregation in Arabidopsis roots. <i>Journal
    of Experimental Botany</i>. 2022;73(8). doi:<a href="https://doi.org/10.1093/jxb/erac019">10.1093/jxb/erac019</a>
  apa: Wang, R., Himschoot, E., Grenzi, M., Chen, J., Safi, A., Krebs, M., … Vanneste,
    S. (2022). Auxin analog-induced Ca2+ signaling is independent of inhibition of
    endosomal aggregation in Arabidopsis roots. <i>Journal of Experimental Botany</i>.
    Oxford University Press. <a href="https://doi.org/10.1093/jxb/erac019">https://doi.org/10.1093/jxb/erac019</a>
  chicago: Wang, R, E Himschoot, M Grenzi, J Chen, A Safi, M Krebs, K Schumacher,
    et al. “Auxin Analog-Induced Ca2+ Signaling Is Independent of Inhibition of Endosomal
    Aggregation in Arabidopsis Roots.” <i>Journal of Experimental Botany</i>. Oxford
    University Press, 2022. <a href="https://doi.org/10.1093/jxb/erac019">https://doi.org/10.1093/jxb/erac019</a>.
  ieee: R. Wang <i>et al.</i>, “Auxin analog-induced Ca2+ signaling is independent
    of inhibition of endosomal aggregation in Arabidopsis roots,” <i>Journal of Experimental
    Botany</i>, vol. 73, no. 8. Oxford University Press, 2022.
  ista: Wang R, Himschoot E, Grenzi M, Chen J, Safi A, Krebs M, Schumacher K, Nowack
    M, Moeder W, Yoshioka K, Van Damme D, De Smet I, Geelen D, Beeckman T, Friml J,
    Costa A, Vanneste S. 2022. Auxin analog-induced Ca2+ signaling is independent
    of inhibition of endosomal aggregation in Arabidopsis roots. Journal of Experimental
    Botany. 73(8), erac019.
  mla: Wang, R., et al. “Auxin Analog-Induced Ca2+ Signaling Is Independent of Inhibition
    of Endosomal Aggregation in Arabidopsis Roots.” <i>Journal of Experimental Botany</i>,
    vol. 73, no. 8, erac019, Oxford University Press, 2022, doi:<a href="https://doi.org/10.1093/jxb/erac019">10.1093/jxb/erac019</a>.
  short: R. Wang, E. Himschoot, M. Grenzi, J. Chen, A. Safi, M. Krebs, K. Schumacher,
    M. Nowack, W. Moeder, K. Yoshioka, D. Van Damme, I. De Smet, D. Geelen, T. Beeckman,
    J. Friml, A. Costa, S. Vanneste, Journal of Experimental Botany 73 (2022).
date_created: 2022-02-03T09:19:01Z
date_published: 2022-04-18T00:00:00Z
date_updated: 2025-05-14T11:06:37Z
day: '18'
department:
- _id: JiFr
doi: 10.1093/jxb/erac019
ec_funded: 1
external_id:
  isi:
  - '000764220900001'
  pmid:
  - '35085386'
intvolume: '        73'
isi: 1
issue: '8'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://biblio.ugent.be/publication/8738721
month: '04'
oa: 1
oa_version: Submitted Version
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '742985'
  name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Journal of Experimental Botany
publication_identifier:
  eissn:
  - 1460-2431
  issn:
  - 0022-0957
publication_status: published
publisher: Oxford University Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: Auxin analog-induced Ca2+ signaling is independent of inhibition of endosomal
  aggregation in Arabidopsis roots
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 73
year: '2022'
...