--- res: bibo_abstract: - Predicting function from sequence is a central problem of biology. Currently, this is possible only locally in a narrow mutational neighborhood around a wildtype sequence rather than globally from any sequence. Using random mutant libraries, we developed a biophysical model that accounts for multiple features of σ70 binding bacterial promoters to predict constitutive gene expression levels from any sequence. We experimentally and theoretically estimated that 10–20% of random sequences lead to expression and ~80% of non-expressing sequences are one mutation away from a functional promoter. The potential for generating expression from random sequences is so pervasive that selection acts against σ70-RNA polymerase binding sites even within inter-genic, promoter-containing regions. This pervasiveness of σ70-binding sites implies that emergence of promoters is not the limiting step in gene regulatory evolution. Ultimately, the inclusion of novel features of promoter function into a mechanistic model enabled not only more accurate predictions of gene expression levels, but also identified that promoters evolve more rapidly than previously thought.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Mato foaf_name: Lagator, Mato foaf_surname: Lagator foaf_workInfoHomepage: http://www.librecat.org/personId=345D25EC-F248-11E8-B48F-1D18A9856A87 - foaf_Person: foaf_givenName: Srdjan foaf_name: Sarikas, Srdjan foaf_surname: Sarikas foaf_workInfoHomepage: http://www.librecat.org/personId=35F0286E-F248-11E8-B48F-1D18A9856A87 - foaf_Person: foaf_givenName: Magdalena foaf_name: Steinrueck, Magdalena foaf_surname: Steinrueck - foaf_Person: foaf_givenName: David foaf_name: Toledo-Aparicio, David foaf_surname: Toledo-Aparicio - foaf_Person: foaf_givenName: Jonathan P foaf_name: Bollback, Jonathan P foaf_surname: Bollback foaf_workInfoHomepage: http://www.librecat.org/personId=2C6FA9CC-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-4624-4612 - foaf_Person: foaf_givenName: Calin C foaf_name: Guet, Calin C foaf_surname: Guet foaf_workInfoHomepage: http://www.librecat.org/personId=47F8433E-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0001-6220-2052 - foaf_Person: foaf_givenName: Gašper foaf_name: Tkačik, Gašper foaf_surname: Tkačik foaf_workInfoHomepage: http://www.librecat.org/personId=3D494DCA-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-6699-1455 bibo_doi: 10.7554/eLife.64543 bibo_volume: 11 dct_date: 2022^xs_gYear dct_identifier: - UT:000751104400001 dct_isPartOf: - http://id.crossref.org/issn/2050-084X dct_language: eng dct_publisher: eLife Sciences Publications@ dct_title: Predicting bacterial promoter function and evolution from random sequences@ fabio_hasPubmedId: '35080492' ...