---
res:
  bibo_abstract:
  - 5-Carboxycytosine (5caC) is a rare epigenetic modification found in nucleic acids
    of all domains of life. Despite its sparse genomic abundance, 5caC is presumed
    to play essential regulatory roles in transcription, maintenance and base-excision
    processes in DNA. In this work, we utilize nuclear magnetic resonance (NMR) spectroscopy
    to address the effects of 5caC incorporation into canonical DNA strands at multiple
    pH and temperature conditions. Our results demonstrate that 5caC has a pH-dependent
    global destabilizing and a base-pair mobility enhancing local impact on dsDNA,
    albeit without any detectable influence on the ground-state B-DNA structure. Measurement
    of hybridization thermodynamics and kinetics of 5caC-bearing DNA duplexes highlighted
    how acidic environment (pH 5.8 and 4.7) destabilizes the double-stranded structure
    by ∼10–20 kJ mol–1 at 37 °C when compared to the same sample at neutral pH. Protonation
    of 5caC results in a lower activation energy for the dissociation process and
    a higher barrier for annealing. Studies on conformational exchange on the microsecond
    time scale regime revealed a sharply localized base-pair motion involving exclusively
    the modified site and its immediate surroundings. By direct comparison with canonical
    and 5-formylcytosine (5fC)-edited strands, we were able to address the impact
    of the two most oxidized naturally occurring cytosine derivatives in the genome.
    These insights on 5caC’s subtle sensitivity to acidic pH contribute to the long-standing
    questions of its capacity as a substrate in base excision repair processes and
    its purpose as an independent, stable epigenetic mark.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Romeo C. A.
      foaf_name: Dubini, Romeo C. A.
      foaf_surname: Dubini
  - foaf_Person:
      foaf_givenName: Eva
      foaf_name: Korytiaková, Eva
      foaf_surname: Korytiaková
  - foaf_Person:
      foaf_givenName: Thea
      foaf_name: Schinkel, Thea
      foaf_surname: Schinkel
  - foaf_Person:
      foaf_givenName: Pia
      foaf_name: Heinrichs, Pia
      foaf_surname: Heinrichs
  - foaf_Person:
      foaf_givenName: Thomas
      foaf_name: Carell, Thomas
      foaf_surname: Carell
  - foaf_Person:
      foaf_givenName: Petra
      foaf_name: Rovo, Petra
      foaf_surname: Rovo
      foaf_workInfoHomepage: http://www.librecat.org/personId=c316e53f-b965-11eb-b128-bb26acc59c00
    orcid: 0000-0001-8729-7326
  bibo_doi: 10.1021/acsphyschemau.1c00050
  bibo_issue: '3'
  bibo_volume: 2
  dct_date: 2022^xs_gYear
  dct_isPartOf:
  - http://id.crossref.org/issn/2694-2445
  dct_language: eng
  dct_publisher: American Chemical Society@
  dct_title: 1H NMR chemical exchange techniques reveal local and global effects of
    oxidized cytosine derivatives@
  fabio_hasPubmedId: '35637781'
...