---
res:
  bibo_abstract:
  - One of the key questions in understanding plant development is how single cells
    behave in a larger context of the tissue. Therefore, it requires the observation
    of the whole organ with a high spatial- as well as temporal resolution over prolonged
    periods of time, which may cause photo-toxic effects. This protocol shows a plant
    sample preparation method for light-sheet microscopy, which is characterized by
    mounting the plant vertically on the surface of a gel. The plant is mounted in
    such a way that the roots are submerged in a liquid medium while the leaves remain
    in the air. In order to ensure photosynthetic activity of the plant, a custom-made
    lighting system illuminates the leaves. To keep the roots in darkness the water
    surface is covered with sheets of black plastic foil. This method allows long-term
    imaging of plant organ development in standardized conditions. @eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Daniel
      foaf_name: Von Wangenheim, Daniel
      foaf_surname: Von Wangenheim
      foaf_workInfoHomepage: http://www.librecat.org/personId=49E91952-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0002-6862-1247
  - foaf_Person:
      foaf_givenName: Robert
      foaf_name: Hauschild, Robert
      foaf_surname: Hauschild
      foaf_workInfoHomepage: http://www.librecat.org/personId=4E01D6B4-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0001-9843-3522
  - foaf_Person:
      foaf_givenName: Jirí
      foaf_name: Friml, Jirí
      foaf_surname: Friml
      foaf_workInfoHomepage: http://www.librecat.org/personId=4159519E-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0002-8302-7596
  bibo_doi: 10.3791/55044
  bibo_issue: '119'
  bibo_volume: 2017
  dct_date: 2017^xs_gYear
  dct_identifier:
  - UT:000397847200041
  dct_language: eng
  dct_publisher: Journal of Visualized Experiments@
  dct_title: Light sheet fluorescence microscopy of plant roots growing on the surface
    of a gel@
...