{"external_id":{"isi":["000397847200041"]},"month":"01","publisher":"Journal of Visualized Experiments","language":[{"iso":"eng"}],"date_updated":"2025-04-15T06:50:23Z","day":"18","date_created":"2018-12-11T11:50:01Z","status":"public","volume":2017,"pubrep_id":"808","oa_version":"Published Version","article_processing_charge":"No","title":"Light sheet fluorescence microscopy of plant roots growing on the surface of a gel","acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"Bio"}],"date_published":"2017-01-18T00:00:00Z","type":"journal_article","abstract":[{"text":"One of the key questions in understanding plant development is how single cells behave in a larger context of the tissue. Therefore, it requires the observation of the whole organ with a high spatial- as well as temporal resolution over prolonged periods of time, which may cause photo-toxic effects. This protocol shows a plant sample preparation method for light-sheet microscopy, which is characterized by mounting the plant vertically on the surface of a gel. The plant is mounted in such a way that the roots are submerged in a liquid medium while the leaves remain in the air. In order to ensure photosynthetic activity of the plant, a custom-made lighting system illuminates the leaves. To keep the roots in darkness the water surface is covered with sheets of black plastic foil. This method allows long-term imaging of plant organ development in standardized conditions. ","lang":"eng"}],"scopus_import":"1","related_material":{"record":[{"id":"5565","status":"public","relation":"popular_science"}]},"file_date_updated":"2018-12-12T10:16:32Z","publication_status":"published","publist_id":"6302","doi":"10.3791/55044","ec_funded":1,"intvolume":"      2017","issue":"119","article_number":"e55044","citation":{"apa":"von Wangenheim, D., Hauschild, R., &#38; Friml, J. (2017). Light sheet fluorescence microscopy of plant roots growing on the surface of a gel. <i>Journal of Visualized Experiments JoVE</i>. Journal of Visualized Experiments. <a href=\"https://doi.org/10.3791/55044\">https://doi.org/10.3791/55044</a>","mla":"von Wangenheim, Daniel, et al. “Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel.” <i>Journal of Visualized Experiments JoVE</i>, vol. 2017, no. 119, e55044, Journal of Visualized Experiments, 2017, doi:<a href=\"https://doi.org/10.3791/55044\">10.3791/55044</a>.","ieee":"D. von Wangenheim, R. Hauschild, and J. Friml, “Light sheet fluorescence microscopy of plant roots growing on the surface of a gel,” <i>Journal of visualized experiments JoVE</i>, vol. 2017, no. 119. Journal of Visualized Experiments, 2017.","ama":"von Wangenheim D, Hauschild R, Friml J. Light sheet fluorescence microscopy of plant roots growing on the surface of a gel. <i>Journal of visualized experiments JoVE</i>. 2017;2017(119). doi:<a href=\"https://doi.org/10.3791/55044\">10.3791/55044</a>","ista":"von Wangenheim D, Hauschild R, Friml J. 2017. Light sheet fluorescence microscopy of plant roots growing on the surface of a gel. Journal of visualized experiments JoVE. 2017(119), e55044.","chicago":"Wangenheim, Daniel von, Robert Hauschild, and Jiří Friml. “Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel.” <i>Journal of Visualized Experiments JoVE</i>. Journal of Visualized Experiments, 2017. <a href=\"https://doi.org/10.3791/55044\">https://doi.org/10.3791/55044</a>.","short":"D. von Wangenheim, R. Hauschild, J. Friml, Journal of Visualized Experiments JoVE 2017 (2017)."},"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","ddc":["580"],"department":[{"_id":"JiFr"},{"_id":"Bio"}],"oa":1,"_id":"1078","file":[{"file_name":"IST-2017-808-v1+1_2017_VWangenheim_list.pdf","file_size":57678,"creator":"system","access_level":"open_access","date_created":"2018-12-12T10:16:31Z","file_id":"5219","date_updated":"2018-12-12T10:16:31Z","content_type":"application/pdf","relation":"main_file"},{"content_type":"application/pdf","relation":"main_file","date_updated":"2018-12-12T10:16:32Z","date_created":"2018-12-12T10:16:32Z","file_id":"5220","file_name":"IST-2017-808-v1+2_2017_VWangenheim_article.pdf","access_level":"open_access","creator":"system","file_size":1317820}],"author":[{"full_name":"Von Wangenheim, Daniel","last_name":"Von Wangenheim","first_name":"Daniel","orcid":"0000-0002-6862-1247","id":"49E91952-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Hauschild","full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","first_name":"Robert"},{"last_name":"Friml","full_name":"Friml, Jirí","first_name":"Jirí","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"year":"2017","has_accepted_license":"1","publication":"Journal of visualized experiments JoVE","isi":1,"project":[{"_id":"25681D80-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","name":"International IST Postdoc Fellowship Programme","grant_number":"291734"},{"grant_number":"282300","call_identifier":"FP7","_id":"25716A02-B435-11E9-9278-68D0E5697425","name":"Polarity and subcellular dynamics in plants"}]}