Light sheet fluorescence microscopy of plant roots growing on the surface of a gel

article Light sheet fluorescence microscopy of plant roots growing on the surface of a gel published Daniel Von Wangenheim author 49E91952-F248-11E8-B48F-1D18A9856A870000-0002-6862-1247 Robert Hauschild author 4E01D6B4-F248-11E8-B48F-1D18A9856A870000-0001-9843-3522 Jirí Friml author 4159519E-F248-11E8-B48F-1D18A9856A870000-0002-8302-7596 JiFr department Bio department International IST Postdoc Fellowship Programme project Polarity and subcellular dynamics in plants project One of the key questions in understanding plant development is how single cells behave in a larger context of the tissue. Therefore, it requires the observation of the whole organ with a high spatial- as well as temporal resolution over prolonged periods of time, which may cause photo-toxic effects. This protocol shows a plant sample preparation method for light-sheet microscopy, which is characterized by mounting the plant vertically on the surface of a gel. The plant is mounted in such a way that the roots are submerged in a liquid medium while the leaves remain in the air. In order to ensure photosynthetic activity of the plant, a custom-made lighting system illuminates the leaves. To keep the roots in darkness the water surface is covered with sheets of black plastic foil. This method allows long-term imaging of plant organ development in standardized conditions. https://research-explorer.ista.ac.at/download/1078/5219/IST-2017-808-v1+1_2017_VWangenheim_list.pdf application/pdfno https://research-explorer.ista.ac.at/download/1078/5220/IST-2017-808-v1+2_2017_VWangenheim_article.pdf application/pdfno MyJove Corporation2017 eng Journal of Visualized Experiments 00039784720004110.3791/55044 2017119 https://research-explorer.ista.ac.at/record/5565 von Wangenheim D, Hauschild R, Friml J. Light sheet fluorescence microscopy of plant roots growing on the surface of a gel. Journal of Visualized Experiments. 2017;2017(119). doi:<a href="https://doi.org/10.3791/55044">10.3791/55044</a> D. von Wangenheim, R. Hauschild, and J. Friml, “Light sheet fluorescence microscopy of plant roots growing on the surface of a gel,” Journal of Visualized Experiments, vol. 2017, no. 119. MyJove Corporation, 2017. von Wangenheim, D., Hauschild, R., & Friml, J. (2017). Light sheet fluorescence microscopy of plant roots growing on the surface of a gel. Journal of Visualized Experiments. MyJove Corporation. <a href="https://doi.org/10.3791/55044">https://doi.org/10.3791/55044</a> von Wangenheim D, Hauschild R, Friml J. 2017. Light sheet fluorescence microscopy of plant roots growing on the surface of a gel. Journal of Visualized Experiments. 2017(119), e55044. D. von Wangenheim, R. Hauschild, J. Friml, Journal of Visualized Experiments 2017 (2017). Wangenheim, Daniel von, Robert Hauschild, and Jiří Friml. “Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel.” Journal of Visualized Experiments. MyJove Corporation, 2017. <a href="https://doi.org/10.3791/55044">https://doi.org/10.3791/55044</a>. von Wangenheim, Daniel, et al. “Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel.” Journal of Visualized Experiments, vol. 2017, no. 119, e55044, MyJove Corporation, 2017, doi:<a href="https://doi.org/10.3791/55044">10.3791/55044</a>. 10782018-12-11T11:50:01Z2026-08-12T14:11:08Z