article published yes Bernadette Basilico author 36035796-5ACA-11E9-A75E-7AF2E56974250000-0003-1843-3173 Laura Ferrucci author Patrizia Ratano author Maria T. Golia author Alfonso Grimaldi author Maria Rosito author Valentina Ferretti author Ingrid Reverte author Caterina Sanchini author Maria C. Marrone author Maria Giubettini author Valeria De Turris author Debora Salerno author Stefano Garofalo author Marie‐Kim St‐Pierre author Micael Carrier author Massimiliano Renzi author Francesca Pagani author Brijesh Modi author Marcello Raspa author Ferdinando Scavizzi author Cornelius T. Gross author Silvia Marinelli author Marie‐Ève Tremblay author Daniele Caprioli author Laura Maggi author Cristina Limatola author Silvia Di Angelantonio author Davide Ragozzino author GaNo department Microglia cells are active players in regulating synaptic development and plasticity in the brain. However, how they influence the normal functioning of synapses is largely unknown. In this study, we characterized the effects of pharmacological microglia depletion, achieved by administration of PLX5622, on hippocampal CA3-CA1 synapses of adult wild type mice. Following microglial depletion, we observed a reduction of spontaneous and evoked glutamatergic activity associated with a decrease of dendritic spine density. We also observed the appearance of immature synaptic features and higher levels of plasticity. Microglia depleted mice showed a deficit in the acquisition of the Novel Object Recognition task. These events were accompanied by hippocampal astrogliosis, although in the absence ofneuroinflammatory condition. PLX-induced synaptic changes were absent in Cx3cr1−/− mice, highlighting the role of CX3CL1/CX3CR1 axis in microglia control of synaptic functioning. Remarkably, microglia repopulation after PLX5622 withdrawal was associated with the recovery of hippocampal synapses and learning functions. Altogether, these data demonstrate that microglia contribute to normal synaptic functioning in the adult brain and that their removal induces reversible changes in organization and activity of glutamatergic synapses. https://research-explorer.ista.ac.at/download/10818/10819/2021_Glia_Basilico.pdf application/pdfno https://creativecommons.org/licenses/by-nc/4.0/ Wiley2022 eng Cellular and Molecular NeuroscienceNeurology 0894-1491 1098-1136 34661306 00070802580000110.1002/glia.24101 701173-195 B. Basilico, L. Ferrucci, P. Ratano, M.T. Golia, A. Grimaldi, M. Rosito, V. Ferretti, I. Reverte, C. Sanchini, M.C. Marrone, M. Giubettini, V. De Turris, D. Salerno, S. Garofalo, M. St‐Pierre, M. Carrier, M. Renzi, F. Pagani, B. Modi, M. Raspa, F. Scavizzi, C.T. Gross, S. Marinelli, M. Tremblay, D. Caprioli, L. Maggi, C. Limatola, S. Di Angelantonio, D. Ragozzino, Glia 70 (2022) 173–195. Basilico, Bernadette, Laura Ferrucci, Patrizia Ratano, Maria T. Golia, Alfonso Grimaldi, Maria Rosito, Valentina Ferretti, et al. “Microglia Control Glutamatergic Synapses in the Adult Mouse Hippocampus.” <i>Glia</i>. Wiley, 2022. <a href="https://doi.org/10.1002/glia.24101">https://doi.org/10.1002/glia.24101</a>. Basilico, B., Ferrucci, L., Ratano, P., Golia, M. T., Grimaldi, A., Rosito, M., … Ragozzino, D. (2022). Microglia control glutamatergic synapses in the adult mouse hippocampus. <i>Glia</i>. Wiley. <a href="https://doi.org/10.1002/glia.24101">https://doi.org/10.1002/glia.24101</a> Basilico B, Ferrucci L, Ratano P, Golia MT, Grimaldi A, Rosito M, Ferretti V, Reverte I, Sanchini C, Marrone MC, Giubettini M, De Turris V, Salerno D, Garofalo S, St‐Pierre M, Carrier M, Renzi M, Pagani F, Modi B, Raspa M, Scavizzi F, Gross CT, Marinelli S, Tremblay M, Caprioli D, Maggi L, Limatola C, Di Angelantonio S, Ragozzino D. 2022. Microglia control glutamatergic synapses in the adult mouse hippocampus. Glia. 70(1), 173–195. Basilico, Bernadette, et al. “Microglia Control Glutamatergic Synapses in the Adult Mouse Hippocampus.” <i>Glia</i>, vol. 70, no. 1, Wiley, 2022, pp. 173–95, doi:<a href="https://doi.org/10.1002/glia.24101">10.1002/glia.24101</a>. Basilico B, Ferrucci L, Ratano P, et al. Microglia control glutamatergic synapses in the adult mouse hippocampus. <i>Glia</i>. 2022;70(1):173-195. doi:<a href="https://doi.org/10.1002/glia.24101">10.1002/glia.24101</a> B. Basilico <i>et al.</i>, “Microglia control glutamatergic synapses in the adult mouse hippocampus,” <i>Glia</i>, vol. 70, no. 1. Wiley, pp. 173–195, 2022. 108182022-03-04T08:53:37Z2024-10-09T21:04:02Z