---
res:
  bibo_abstract:
  - 'BceRS and PsdRS are paralogous two-component systems in Bacillus subtilis controlling
    the response to antimicrobial peptides. In the presence of extracellular bacitracin
    and nisin, respectively, the two response regulators (RRs) bind their target promoters,
    PbceA or PpsdA, resulting in a strong up-regulation of target gene expression
    and ultimately antibiotic resistance. Despite high sequence similarity between
    the RRs BceR and PsdR and their known binding sites, no cross-regulation has been
    observed between them. We therefore investigated the specificity determinants
    of PbceA and PpsdA that ensure the insulation of these two paralogous pathways
    at the RR–promoter interface. In vivo and in vitro analyses demonstrate that the
    regulatory regions within these two promoters contain three important elements:
    in addition to the known (main) binding site, we identified a linker region and
    a secondary binding site that are crucial for functionality. Initial binding to
    the high-affinity, low-specificity main binding site is a prerequisite for the
    subsequent highly specific binding of a second RR dimer to the low-affinity secondary
    binding site. In addition to this hierarchical cooperative binding, discrimination
    requires a competition of the two RRs for their respective binding site mediated
    by only slight differences in binding affinities.@eng'
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Chong
      foaf_name: Fang, Chong
      foaf_surname: Fang
  - foaf_Person:
      foaf_givenName: Anna A
      foaf_name: Nagy-Staron, Anna A
      foaf_surname: Nagy-Staron
      foaf_workInfoHomepage: http://www.librecat.org/personId=3ABC5BA6-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0002-1391-8377
  - foaf_Person:
      foaf_givenName: Martin
      foaf_name: Grafe, Martin
      foaf_surname: Grafe
  - foaf_Person:
      foaf_givenName: Ralf
      foaf_name: Heermann, Ralf
      foaf_surname: Heermann
  - foaf_Person:
      foaf_givenName: Kirsten
      foaf_name: Jung, Kirsten
      foaf_surname: Jung
  - foaf_Person:
      foaf_givenName: Susanne
      foaf_name: Gebhard, Susanne
      foaf_surname: Gebhard
  - foaf_Person:
      foaf_givenName: Thorsten
      foaf_name: Mascher, Thorsten
      foaf_surname: Mascher
  bibo_doi: 10.1111/mmi.13597
  bibo_issue: '1'
  bibo_volume: 104
  dct_date: 2017^xs_gYear
  dct_identifier:
  - UT:000398059200002
  dct_isPartOf:
  - http://id.crossref.org/issn/ 0950-382X
  dct_language: eng
  dct_publisher: Wiley-Blackwell@
  dct_title: Insulation and wiring specificity of BceR like response regulators and
    their target promoters in Bacillus subtilis@
...