---
_id: '1084'
abstract:
- lang: eng
  text: 'BceRS and PsdRS are paralogous two-component systems in Bacillus subtilis
    controlling the response to antimicrobial peptides. In the presence of extracellular
    bacitracin and nisin, respectively, the two response regulators (RRs) bind their
    target promoters, PbceA or PpsdA, resulting in a strong up-regulation of target
    gene expression and ultimately antibiotic resistance. Despite high sequence similarity
    between the RRs BceR and PsdR and their known binding sites, no cross-regulation
    has been observed between them. We therefore investigated the specificity determinants
    of PbceA and PpsdA that ensure the insulation of these two paralogous pathways
    at the RR–promoter interface. In vivo and in vitro analyses demonstrate that the
    regulatory regions within these two promoters contain three important elements:
    in addition to the known (main) binding site, we identified a linker region and
    a secondary binding site that are crucial for functionality. Initial binding to
    the high-affinity, low-specificity main binding site is a prerequisite for the
    subsequent highly specific binding of a second RR dimer to the low-affinity secondary
    binding site. In addition to this hierarchical cooperative binding, discrimination
    requires a competition of the two RRs for their respective binding site mediated
    by only slight differences in binding affinities.'
article_processing_charge: No
author:
- first_name: Chong
  full_name: Fang, Chong
  last_name: Fang
- first_name: Anna A
  full_name: Nagy-Staron, Anna A
  id: 3ABC5BA6-F248-11E8-B48F-1D18A9856A87
  last_name: Nagy-Staron
  orcid: 0000-0002-1391-8377
- first_name: Martin
  full_name: Grafe, Martin
  last_name: Grafe
- first_name: Ralf
  full_name: Heermann, Ralf
  last_name: Heermann
- first_name: Kirsten
  full_name: Jung, Kirsten
  last_name: Jung
- first_name: Susanne
  full_name: Gebhard, Susanne
  last_name: Gebhard
- first_name: Thorsten
  full_name: Mascher, Thorsten
  last_name: Mascher
citation:
  ama: Fang C, Nagy-Staron AA, Grafe M, et al. Insulation and wiring specificity of
    BceR like response regulators and their target promoters in Bacillus subtilis.
    <i>Molecular Microbiology</i>. 2017;104(1):16-31. doi:<a href="https://doi.org/10.1111/mmi.13597">10.1111/mmi.13597</a>
  apa: Fang, C., Nagy-Staron, A. A., Grafe, M., Heermann, R., Jung, K., Gebhard, S.,
    &#38; Mascher, T. (2017). Insulation and wiring specificity of BceR like response
    regulators and their target promoters in Bacillus subtilis. <i>Molecular Microbiology</i>.
    Wiley-Blackwell. <a href="https://doi.org/10.1111/mmi.13597">https://doi.org/10.1111/mmi.13597</a>
  chicago: Fang, Chong, Anna A Nagy-Staron, Martin Grafe, Ralf Heermann, Kirsten Jung,
    Susanne Gebhard, and Thorsten Mascher. “Insulation and Wiring Specificity of BceR
    like Response Regulators and Their Target Promoters in Bacillus Subtilis.” <i>Molecular
    Microbiology</i>. Wiley-Blackwell, 2017. <a href="https://doi.org/10.1111/mmi.13597">https://doi.org/10.1111/mmi.13597</a>.
  ieee: C. Fang <i>et al.</i>, “Insulation and wiring specificity of BceR like response
    regulators and their target promoters in Bacillus subtilis,” <i>Molecular Microbiology</i>,
    vol. 104, no. 1. Wiley-Blackwell, pp. 16–31, 2017.
  ista: Fang C, Nagy-Staron AA, Grafe M, Heermann R, Jung K, Gebhard S, Mascher T.
    2017. Insulation and wiring specificity of BceR like response regulators and their
    target promoters in Bacillus subtilis. Molecular Microbiology. 104(1), 16–31.
  mla: Fang, Chong, et al. “Insulation and Wiring Specificity of BceR like Response
    Regulators and Their Target Promoters in Bacillus Subtilis.” <i>Molecular Microbiology</i>,
    vol. 104, no. 1, Wiley-Blackwell, 2017, pp. 16–31, doi:<a href="https://doi.org/10.1111/mmi.13597">10.1111/mmi.13597</a>.
  short: C. Fang, A.A. Nagy-Staron, M. Grafe, R. Heermann, K. Jung, S. Gebhard, T.
    Mascher, Molecular Microbiology 104 (2017) 16–31.
date_created: 2018-12-11T11:50:03Z
date_published: 2017-04-01T00:00:00Z
date_updated: 2026-04-16T09:56:09Z
day: '01'
department:
- _id: CaGu
doi: 10.1111/mmi.13597
external_id:
  isi:
  - '000398059200002'
intvolume: '       104'
isi: 1
issue: '1'
language:
- iso: eng
month: '04'
oa_version: None
page: 16 - 31
publication: Molecular Microbiology
publication_identifier:
  issn:
  - ' 0950-382X'
publication_status: published
publisher: Wiley-Blackwell
publist_id: '6294'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Insulation and wiring specificity of BceR like response regulators and their
  target promoters in Bacillus subtilis
type: journal_article
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
volume: 104
year: '2017'
...