--- res: bibo_abstract: - Despite the growing interest in using chemical genetics in plant research, small molecule target identification remains a major challenge. The cellular thermal shift assay coupled with high-resolution mass spectrometry (CETSA MS) that monitors changes in the thermal stability of proteins caused by their interactions with small molecules, other proteins, or posttranslational modifications, allows the discovery of drug targets or the study of protein–metabolite and protein–protein interactions mainly in mammalian cells. To showcase the applicability of this method in plants, we applied CETSA MS to intact Arabidopsis thaliana cells and identified the thermal proteome of the plant-specific glycogen synthase kinase 3 (GSK3) inhibitor, bikinin. A comparison between the thermal and the phosphoproteomes of bikinin revealed the auxin efflux carrier PIN-FORMED1 (PIN1) as a substrate of the Arabidopsis GSK3s that negatively regulate the brassinosteroid signaling. We established that PIN1 phosphorylation by the GSK3s is essential for maintaining its intracellular polarity that is required for auxin-mediated regulation of vascular patterning in the leaf, thus revealing cross-talk between brassinosteroid and auxin signaling.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Qing foaf_name: Lu, Qing foaf_surname: Lu - foaf_Person: foaf_givenName: Yonghong foaf_name: Zhang, Yonghong foaf_surname: Zhang - foaf_Person: foaf_givenName: Joakim foaf_name: Hellner, Joakim foaf_surname: Hellner - foaf_Person: foaf_givenName: Caterina foaf_name: Giannini, Caterina foaf_surname: Giannini foaf_workInfoHomepage: http://www.librecat.org/personId=e3fdddd5-f6e0-11ea-865d-ca99ee6367f4 - foaf_Person: foaf_givenName: Xiangyu foaf_name: Xu, Xiangyu foaf_surname: Xu - foaf_Person: foaf_givenName: Jarne foaf_name: Pauwels, Jarne foaf_surname: Pauwels - foaf_Person: foaf_givenName: Qian foaf_name: Ma, Qian foaf_surname: Ma - foaf_Person: foaf_givenName: Wim foaf_name: Dejonghe, Wim foaf_surname: Dejonghe - foaf_Person: foaf_givenName: Huibin foaf_name: Han, Huibin foaf_surname: Han foaf_workInfoHomepage: http://www.librecat.org/personId=31435098-F248-11E8-B48F-1D18A9856A87 - foaf_Person: foaf_givenName: Brigitte foaf_name: Van De Cotte, Brigitte foaf_surname: Van De Cotte - foaf_Person: foaf_givenName: Francis foaf_name: Impens, Francis foaf_surname: Impens - foaf_Person: foaf_givenName: Kris foaf_name: Gevaert, Kris foaf_surname: Gevaert - foaf_Person: foaf_givenName: Ive foaf_name: De Smet, Ive foaf_surname: De Smet - foaf_Person: foaf_givenName: Jiří foaf_name: Friml, Jiří foaf_surname: Friml foaf_workInfoHomepage: http://www.librecat.org/personId=4159519E-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-8302-7596 - foaf_Person: foaf_givenName: Daniel Martinez foaf_name: Molina, Daniel Martinez foaf_surname: Molina - foaf_Person: foaf_givenName: Eugenia foaf_name: Russinova, Eugenia foaf_surname: Russinova bibo_doi: 10.1073/pnas.2118220119 bibo_issue: '11' bibo_volume: 119 dct_date: 2022^xs_gYear dct_identifier: - UT:000771756300008 dct_isPartOf: - http://id.crossref.org/issn/1091-6490 dct_language: eng dct_publisher: Proceedings of the National Academy of Sciences@ dct_title: Proteome-wide cellular thermal shift assay reveals unexpected cross-talk between brassinosteroid and auxin signaling@ fabio_hasPubmedId: '35254915' ...