---
res:
  bibo_abstract:
  - Genetically encoded tags have introduced extensive lines of application from purification
    of tagged proteins to their visualization at the single molecular, cellular, histological
    and whole-body levels. Combined with other rapidly developing technologies such
    as clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated
    protein 9 (Cas9) system, proteomics, super-resolution microscopy and proximity
    labeling, a large variety of genetically encoded tags have been developed in the
    last two decades. In this review, I focus on the current status of tag development
    for electron microscopic (EM) visualization of proteins with metal particle labeling.
    Compared with conventional immunoelectron microscopy using gold particles, tag-mediated
    metal particle labeling has several advantages that could potentially improve
    the sensitivity, spatial and temporal resolution, and applicability to a wide
    range of proteins of interest (POIs). It may enable researchers to detect single
    molecules in situ, allowing the quantitative measurement of absolute numbers and
    exact localization patterns of POI in the ultrastructural context. Thus, genetically
    encoded tags for EM could revolutionize the field as green fluorescence protein
    did for light microscopy, although we still have many challenges to overcome before
    reaching this goal.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Ryuichi
      foaf_name: Shigemoto, Ryuichi
      foaf_surname: Shigemoto
      foaf_workInfoHomepage: http://www.librecat.org/personId=499F3ABC-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0001-8761-9444
  bibo_doi: 10.1093/jmicro/dfab048
  bibo_issue: Supplement_1
  bibo_volume: 71
  dct_date: 2022^xs_gYear
  dct_identifier:
  - UT:000768384100011
  dct_isPartOf:
  - http://id.crossref.org/issn/2050-5698
  - http://id.crossref.org/issn/2050-5701
  dct_language: eng
  dct_publisher: Oxford University Press@
  dct_title: Electron microscopic visualization of single molecules by tag-mediated
    metal particle labeling@
  fabio_hasPubmedId: '35275179'
...