article published yes Kohgaku Eguchi author 2B7846DC-F248-11E8-B48F-1D18A9856A870000-0002-6170-2546 Jacqueline-Claire Montanaro-Punzengruber author 3786AB44-F248-11E8-B48F-1D18A9856A87 Elodie Le Monnier author 3B59276A-F248-11E8-B48F-1D18A9856A87 Ryuichi Shigemoto author 499F3ABC-F248-11E8-B48F-1D18A9856A870000-0001-8761-9444 RySh department In situ analysis of single channel subunit composition in neurons: physiological implication in synaptic plasticity and behaviour project LGI1 antibody-induced pathophysiology in synapses project Upon the arrival of action potentials at nerve terminals, neurotransmitters are released from synaptic vesicles (SVs) by exocytosis. CaV2.1, 2.2, and 2.3 are the major subunits of the voltage-gated calcium channel (VGCC) responsible for increasing intraterminal calcium levels and triggering SV exocytosis in the central nervous system (CNS) synapses. The two-dimensional analysis of CaV2 distributions using sodium dodecyl sulfate (SDS)-digested freeze-fracture replica labeling (SDS-FRL) has revealed their numbers, densities, and nanoscale clustering patterns in individual presynaptic active zones. The variation in these properties affects the coupling of VGCCs with calcium sensors on SVs, synaptic efficacy, and temporal precision of transmission. In this study, we summarize how the morphological parameters of CaV2 distribution obtained using SDS-FRL differ depending on the different types of synapses and could correspond to functional properties in synaptic transmission. https://research-explorer.ista.ac.at/download/10890/10911/2022_FrontiersNeuroanatomy_Eguchi.pdf application/pdfno Frontiers2022 eng 1662-5129 35280978 00076666270000110.3389/fnana.2022.846615 16 Eguchi K, Montanaro-Punzengruber J-C, Le Monnier E, Shigemoto R. 2022. The number and distinct clustering patterns of voltage-gated Calcium channels in nerve terminals. Frontiers in Neuroanatomy. 16, 846615. Eguchi, Kohgaku, et al. “The Number and Distinct Clustering Patterns of Voltage-Gated Calcium Channels in Nerve Terminals.” <i>Frontiers in Neuroanatomy</i>, vol. 16, 846615, Frontiers, 2022, doi:<a href="https://doi.org/10.3389/fnana.2022.846615">10.3389/fnana.2022.846615</a>. K. Eguchi, J.-C. Montanaro-Punzengruber, E. Le Monnier, and R. Shigemoto, “The number and distinct clustering patterns of voltage-gated Calcium channels in nerve terminals,” <i>Frontiers in Neuroanatomy</i>, vol. 16. Frontiers, 2022. Eguchi, Kohgaku, Jacqueline-Claire Montanaro-Punzengruber, Elodie Le Monnier, and Ryuichi Shigemoto. “The Number and Distinct Clustering Patterns of Voltage-Gated Calcium Channels in Nerve Terminals.” <i>Frontiers in Neuroanatomy</i>. Frontiers, 2022. <a href="https://doi.org/10.3389/fnana.2022.846615">https://doi.org/10.3389/fnana.2022.846615</a>. Eguchi K, Montanaro-Punzengruber J-C, Le Monnier E, Shigemoto R. The number and distinct clustering patterns of voltage-gated Calcium channels in nerve terminals. <i>Frontiers in Neuroanatomy</i>. 2022;16. doi:<a href="https://doi.org/10.3389/fnana.2022.846615">10.3389/fnana.2022.846615</a> Eguchi, K., Montanaro-Punzengruber, J.-C., Le Monnier, E., &#38; Shigemoto, R. (2022). The number and distinct clustering patterns of voltage-gated Calcium channels in nerve terminals. <i>Frontiers in Neuroanatomy</i>. Frontiers. <a href="https://doi.org/10.3389/fnana.2022.846615">https://doi.org/10.3389/fnana.2022.846615</a> K. Eguchi, J.-C. Montanaro-Punzengruber, E. Le Monnier, R. Shigemoto, Frontiers in Neuroanatomy 16 (2022). 108902022-03-20T23:01:39Z2026-04-16T08:18:54Z