article published yes Shamsi Emtenani author 49D32318-F248-11E8-B48F-1D18A9856A870000-0001-6981-6938 Elliot T Martin author Attila György author 3BCEDBE0-F248-11E8-B48F-1D18A9856A870000-0002-1819-198X Julia Bicher author 3CCBB46E-F248-11E8-B48F-1D18A9856A87 Jakob-Wendelin Genger author Thomas Köcher author Maria Akhmanova author 3425EC26-F248-11E8-B48F-1D18A9856A870000-0003-1522-3162 Mariana Pereira Guarda author 6de81d9d-e2f2-11eb-945a-af8bc2a60b260000-0001-8238-480X Marko Roblek author 3047D808-F248-11E8-B48F-1D18A9856A870000-0001-9588-1389 Andreas Bergthaler author Thomas R Hurd author Prashanth Rangan author Daria E Siekhaus author 3D224B9E-F248-11E8-B48F-1D18A9856A870000-0001-8323-8353 DaSi department LoSw department Investigating the role of transporters in invasive migration through junctions project Modeling epithelial tissue mechanics during cell invasion project The role of Drosophila TNF alpha in immune cell invasion project Cellular metabolism must adapt to changing demands to enable homeostasis. During immune responses or cancer metastasis, cells leading migration into challenging environments require an energy boost, but what controls this capacity is unclear. Here, we study a previously uncharacterized nuclear protein, Atossa (encoded by CG9005), which supports macrophage invasion into the germband of Drosophila by controlling cellular metabolism. First, nuclear Atossa increases mRNA levels of Porthos, a DEAD-box protein, and of two metabolic enzymes, lysine-α-ketoglutarate reductase (LKR/SDH) and NADPH glyoxylate reductase (GR/HPR), thus enhancing mitochondrial bioenergetics. Then Porthos supports ribosome assembly and thereby raises the translational efficiency of a subset of mRNAs, including those affecting mitochondrial functions, the electron transport chain, and metabolism. Mitochondrial respiration measurements, metabolomics, and live imaging indicate that Atossa and Porthos power up OxPhos and energy production to promote the forging of a path into tissues by leading macrophages. Since many crucial physiological responses require increases in mitochondrial energy output, this previously undescribed genetic program may modulate a wide range of cellular behaviors. https://research-explorer.ista.ac.at/download/10918/10919/Macrophage mitochondrial bioenergetics and tissue invasion are boosted by an Atossa-Porthos axis in Drosopila.pdf application/pdfno Embo Press2022 eng 1460-2075 35319107 00077195700000110.15252/embj.2021109049 41 Emtenani, Shamsi, et al. “Macrophage Mitochondrial Bioenergetics and Tissue Invasion Are Boosted by an Atossa-Porthos Axis in Drosophila.” <i>The Embo Journal</i>, vol. 41, e109049, Embo Press, 2022, doi:<a href="https://doi.org/10.15252/embj.2021109049">10.15252/embj.2021109049</a>. S. Emtenani, E.T. Martin, A. György, J. Bicher, J.-W. Genger, T. Köcher, M. Akhmanova, M. Pereira Guarda, M. Roblek, A. Bergthaler, T.R. Hurd, P. Rangan, D.E. Siekhaus, The Embo Journal 41 (2022). Emtenani, Shamsi, Elliot T Martin, Attila György, Julia Bicher, Jakob-Wendelin Genger, Thomas Köcher, Maria Akhmanova, et al. “Macrophage Mitochondrial Bioenergetics and Tissue Invasion Are Boosted by an Atossa-Porthos Axis in Drosophila.” <i>The Embo Journal</i>. Embo Press, 2022. <a href="https://doi.org/10.15252/embj.2021109049">https://doi.org/10.15252/embj.2021109049</a>. Emtenani, S., Martin, E. T., György, A., Bicher, J., Genger, J.-W., Köcher, T., … Siekhaus, D. E. (2022). Macrophage mitochondrial bioenergetics and tissue invasion are boosted by an Atossa-Porthos axis in Drosophila. <i>The Embo Journal</i>. Embo Press. <a href="https://doi.org/10.15252/embj.2021109049">https://doi.org/10.15252/embj.2021109049</a> Emtenani S, Martin ET, György A, Bicher J, Genger J-W, Köcher T, Akhmanova M, Pereira Guarda M, Roblek M, Bergthaler A, Hurd TR, Rangan P, Siekhaus DE. 2022. Macrophage mitochondrial bioenergetics and tissue invasion are boosted by an Atossa-Porthos axis in Drosophila. The Embo Journal. 41, e109049. Emtenani S, Martin ET, György A, et al. Macrophage mitochondrial bioenergetics and tissue invasion are boosted by an Atossa-Porthos axis in Drosophila. <i>The Embo Journal</i>. 2022;41. doi:<a href="https://doi.org/10.15252/embj.2021109049">10.15252/embj.2021109049</a> S. Emtenani <i>et al.</i>, “Macrophage mitochondrial bioenergetics and tissue invasion are boosted by an Atossa-Porthos axis in Drosophila,” <i>The Embo Journal</i>, vol. 41. Embo Press, 2022. 109182022-03-24T13:23:09Z2025-06-12T06:20:16Z