--- res: bibo_abstract: - During metazoan development, the temporal pattern of morphogen signaling is critical for organizing cell fates in space and time. Yet, tools for temporally controlling morphogen signaling within the embryo are still scarce. Here, we developed a photoactivatable Nodal receptor to determine how the temporal pattern of Nodal signaling affects cell fate specification during zebrafish gastrulation. By using this receptor to manipulate the duration of Nodal signaling in vivo by light, we show that extended Nodal signaling within the organizer promotes prechordal plate specification and suppresses endoderm differentiation. Endoderm differentiation is suppressed by extended Nodal signaling inducing expression of the transcriptional repressor goosecoid (gsc) in prechordal plate progenitors, which in turn restrains Nodal signaling from upregulating the endoderm differentiation gene sox17 within these cells. Thus, optogenetic manipulation of Nodal signaling identifies a critical role of Nodal signaling duration for organizer cell fate specification during gastrulation.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Keisuke foaf_name: Sako, Keisuke foaf_surname: Sako foaf_workInfoHomepage: http://www.librecat.org/personId=3BED66BE-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-6453-8075 - foaf_Person: foaf_givenName: Saurabh foaf_name: Pradhan, Saurabh foaf_surname: Pradhan - foaf_Person: foaf_givenName: Vanessa foaf_name: Barone, Vanessa foaf_surname: Barone foaf_workInfoHomepage: http://www.librecat.org/personId=419EECCC-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0003-2676-3367 - foaf_Person: foaf_givenName: Álvaro foaf_name: Inglés Prieto, Álvaro foaf_surname: Inglés Prieto foaf_workInfoHomepage: http://www.librecat.org/personId=2A9DB292-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-5409-8571 - foaf_Person: foaf_givenName: Patrick foaf_name: Mueller, Patrick foaf_surname: Mueller - foaf_Person: foaf_givenName: Verena foaf_name: Ruprecht, Verena foaf_surname: Ruprecht foaf_workInfoHomepage: http://www.librecat.org/personId=4D71A03A-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0003-4088-8633 - foaf_Person: foaf_givenName: Daniel foaf_name: Capek, Daniel foaf_surname: Capek foaf_workInfoHomepage: http://www.librecat.org/personId=31C42484-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0001-5199-9940 - foaf_Person: foaf_givenName: Sanjeev foaf_name: Galande, Sanjeev foaf_surname: Galande - foaf_Person: foaf_givenName: Harald L foaf_name: Janovjak, Harald L foaf_surname: Janovjak foaf_workInfoHomepage: http://www.librecat.org/personId=33BA6C30-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-8023-9315 - foaf_Person: foaf_givenName: Carl-Philipp J foaf_name: Heisenberg, Carl-Philipp J foaf_surname: Heisenberg foaf_workInfoHomepage: http://www.librecat.org/personId=39427864-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-0912-4566 bibo_doi: 10.1016/j.celrep.2016.06.036 bibo_issue: '3' bibo_volume: 16 dct_date: 2016^xs_gYear dct_language: eng dct_publisher: Cell Press@ dct_title: Optogenetic control of nodal signaling reveals a temporal pattern of nodal signaling regulating cell fate specification during gastrulation@ ...