article published yes Keisuke Sako author 3BED66BE-F248-11E8-B48F-1D18A9856A870000-0002-6453-8075 Saurabh Pradhan author Vanessa Barone author 419EECCC-F248-11E8-B48F-1D18A9856A870000-0003-2676-3367 Álvaro Inglés Prieto author 2A9DB292-F248-11E8-B48F-1D18A9856A870000-0002-5409-8571 Patrick Mueller author Verena Ruprecht author 4D71A03A-F248-11E8-B48F-1D18A9856A870000-0003-4088-8633 Daniel Capek author 31C42484-F248-11E8-B48F-1D18A9856A870000-0001-5199-9940 Sanjeev Galande author Harald L Janovjak author 33BA6C30-F248-11E8-B48F-1D18A9856A870000-0002-8023-9315 Carl-Philipp J Heisenberg author 39427864-F248-11E8-B48F-1D18A9856A870000-0002-0912-4566 CaHe department HaJa department Cell- and Tissue Mechanics in Zebrafish Germ Layer Formation project Cell Cortex and Germ Layer Formation in Zebrafish Gastrulation project Microbial Ion Channels for Synthetic Neurobiology project During metazoan development, the temporal pattern of morphogen signaling is critical for organizing cell fates in space and time. Yet, tools for temporally controlling morphogen signaling within the embryo are still scarce. Here, we developed a photoactivatable Nodal receptor to determine how the temporal pattern of Nodal signaling affects cell fate specification during zebrafish gastrulation. By using this receptor to manipulate the duration of Nodal signaling in vivo by light, we show that extended Nodal signaling within the organizer promotes prechordal plate specification and suppresses endoderm differentiation. Endoderm differentiation is suppressed by extended Nodal signaling inducing expression of the transcriptional repressor goosecoid (gsc) in prechordal plate progenitors, which in turn restrains Nodal signaling from upregulating the endoderm differentiation gene sox17 within these cells. Thus, optogenetic manipulation of Nodal signaling identifies a critical role of Nodal signaling duration for organizer cell fate specification during gastrulation. https://research-explorer.ista.ac.at/download/1100/4857/IST-2017-754-v1+1_1-s2.0-S2211124716307768-main.pdf application/pdfno Cell Press2016 eng 00038026420002410.1016/j.celrep.2016.06.036 163866 - 877 https://research-explorer.ista.ac.at/record/961 https://research-explorer.ista.ac.at/record/50 Sako, Keisuke, Saurabh Pradhan, Vanessa Barone, Álvaro Inglés Prieto, Patrick Mueller, Verena Ruprecht, Daniel Capek, Sanjeev Galande, Harald L Janovjak, and Carl-Philipp J Heisenberg. “Optogenetic Control of Nodal Signaling Reveals a Temporal Pattern of Nodal Signaling Regulating Cell Fate Specification during Gastrulation.” <i>Cell Reports</i>. Cell Press, 2016. <a href="https://doi.org/10.1016/j.celrep.2016.06.036">https://doi.org/10.1016/j.celrep.2016.06.036</a>. Sako K, Pradhan S, Barone V, et al. Optogenetic control of nodal signaling reveals a temporal pattern of nodal signaling regulating cell fate specification during gastrulation. <i>Cell Reports</i>. 2016;16(3):866-877. doi:<a href="https://doi.org/10.1016/j.celrep.2016.06.036">10.1016/j.celrep.2016.06.036</a> Sako, K., Pradhan, S., Barone, V., Inglés Prieto, Á., Mueller, P., Ruprecht, V., … Heisenberg, C.-P. J. (2016). Optogenetic control of nodal signaling reveals a temporal pattern of nodal signaling regulating cell fate specification during gastrulation. <i>Cell Reports</i>. Cell Press. <a href="https://doi.org/10.1016/j.celrep.2016.06.036">https://doi.org/10.1016/j.celrep.2016.06.036</a> Sako, Keisuke, et al. “Optogenetic Control of Nodal Signaling Reveals a Temporal Pattern of Nodal Signaling Regulating Cell Fate Specification during Gastrulation.” <i>Cell Reports</i>, vol. 16, no. 3, Cell Press, 2016, pp. 866–77, doi:<a href="https://doi.org/10.1016/j.celrep.2016.06.036">10.1016/j.celrep.2016.06.036</a>. K. Sako <i>et al.</i>, “Optogenetic control of nodal signaling reveals a temporal pattern of nodal signaling regulating cell fate specification during gastrulation,” <i>Cell Reports</i>, vol. 16, no. 3. Cell Press, pp. 866–877, 2016. Sako K, Pradhan S, Barone V, Inglés Prieto Á, Mueller P, Ruprecht V, Capek D, Galande S, Janovjak HL, Heisenberg C-PJ. 2016. Optogenetic control of nodal signaling reveals a temporal pattern of nodal signaling regulating cell fate specification during gastrulation. Cell Reports. 16(3), 866–877. K. Sako, S. Pradhan, V. Barone, Á. Inglés Prieto, P. Mueller, V. Ruprecht, D. Capek, S. Galande, H.L. Janovjak, C.-P.J. Heisenberg, Cell Reports 16 (2016) 866–877. 11002018-12-11T11:50:08Z2026-05-12T22:31:15Z