---
res:
  bibo_abstract:
  - Optical sensors based on the phenomenon of Förster resonance energy transfer (FRET)
    are powerful tools that have advanced the study of small molecules in biological
    systems. However, sensor construction is not trivial and often requires multiple
    rounds of engineering or an ability to screen large numbers of variants. A method
    that would allow the accurate rational design of FRET sensors would expedite the
    production of biologically useful sensors. Here, we present Rangefinder, a computational
    algorithm that allows rapid in silico screening of dye attachment sites in a ligand-binding
    protein for the conjugation of a dye molecule to act as a Förster acceptor for
    a fused fluorescent protein. We present three ratiometric fluorescent sensors
    designed with Rangefinder, including a maltose sensor with a dynamic range of
    >300% and the first sensors for the most abundant sialic acid in human cells,
    N-acetylneuraminic acid. Provided a ligand-binding protein exists, it is our expectation
    that this model will facilitate the design of an optical sensor for any small
    molecule of interest.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Joshua
      foaf_name: Mitchell, Joshua
      foaf_surname: Mitchell
  - foaf_Person:
      foaf_givenName: Jason
      foaf_name: Whitfield, Jason
      foaf_surname: Whitfield
  - foaf_Person:
      foaf_givenName: William
      foaf_name: Zhang, William
      foaf_surname: Zhang
  - foaf_Person:
      foaf_givenName: Christian
      foaf_name: Henneberger, Christian
      foaf_surname: Henneberger
  - foaf_Person:
      foaf_givenName: Harald L
      foaf_name: Janovjak, Harald L
      foaf_surname: Janovjak
      foaf_workInfoHomepage: http://www.librecat.org/personId=33BA6C30-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0002-8023-9315
  - foaf_Person:
      foaf_givenName: Megan
      foaf_name: O'Mara, Megan
      foaf_surname: O'Mara
  - foaf_Person:
      foaf_givenName: Colin
      foaf_name: Jackson, Colin
      foaf_surname: Jackson
  bibo_doi: 10.1021/acssensors.6b00576
  bibo_issue: '11'
  bibo_volume: 1
  dct_date: 2016^xs_gYear
  dct_identifier:
  - UT:000388914800003
  dct_language: eng
  dct_publisher: American Chemical Society@
  dct_title: 'Rangefinder: A semisynthetic FRET sensor design algorithm@'
...