---
_id: '11052'
abstract:
- lang: eng
  text: In order to combat molecular damage, most cellular proteins undergo rapid
    turnover. We have previously identified large nuclear protein assemblies that
    can persist for years in post-mitotic tissues and are subject to age-related decline.
    Here, we report that mitochondria can be long lived in the mouse brain and reveal
    that specific mitochondrial proteins have half-lives longer than the average proteome.
    These mitochondrial long-lived proteins (mitoLLPs) are core components of the
    electron transport chain (ETC) and display increased longevity in respiratory
    supercomplexes. We find that COX7C, a mitoLLP that forms a stable contact site
    between complexes I and IV, is required for complex IV and supercomplex assembly.
    Remarkably, even upon depletion of COX7C transcripts, ETC function is maintained
    for days, effectively uncoupling mitochondrial function from ongoing transcription
    of its mitoLLPs. Our results suggest that modulating protein longevity within
    the ETC is critical for mitochondrial proteome maintenance and the robustness
    of mitochondrial function.
article_processing_charge: No
article_type: original
author:
- first_name: Shefali
  full_name: Krishna, Shefali
  last_name: Krishna
- first_name: Rafael
  full_name: Arrojo e Drigo, Rafael
  last_name: Arrojo e Drigo
- first_name: Juliana S.
  full_name: Capitanio, Juliana S.
  last_name: Capitanio
- first_name: Ranjan
  full_name: Ramachandra, Ranjan
  last_name: Ramachandra
- first_name: Mark
  full_name: Ellisman, Mark
  last_name: Ellisman
- first_name: Martin W
  full_name: HETZER, Martin W
  id: 86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed
  last_name: HETZER
  orcid: 0000-0002-2111-992X
citation:
  ama: Krishna S, Arrojo e Drigo R, Capitanio JS, Ramachandra R, Ellisman M, Hetzer
    M. Identification of long-lived proteins in the mitochondria reveals increased
    stability of the electron transport chain. <i>Developmental Cell</i>. 2021;56(21):P2952-2965.e9.
    doi:<a href="https://doi.org/10.1016/j.devcel.2021.10.008">10.1016/j.devcel.2021.10.008</a>
  apa: Krishna, S., Arrojo e Drigo, R., Capitanio, J. S., Ramachandra, R., Ellisman,
    M., &#38; Hetzer, M. (2021). Identification of long-lived proteins in the mitochondria
    reveals increased stability of the electron transport chain. <i>Developmental
    Cell</i>. Elsevier. <a href="https://doi.org/10.1016/j.devcel.2021.10.008">https://doi.org/10.1016/j.devcel.2021.10.008</a>
  chicago: Krishna, Shefali, Rafael Arrojo e Drigo, Juliana S. Capitanio, Ranjan Ramachandra,
    Mark Ellisman, and Martin Hetzer. “Identification of Long-Lived Proteins in the
    Mitochondria Reveals Increased Stability of the Electron Transport Chain.” <i>Developmental
    Cell</i>. Elsevier, 2021. <a href="https://doi.org/10.1016/j.devcel.2021.10.008">https://doi.org/10.1016/j.devcel.2021.10.008</a>.
  ieee: S. Krishna, R. Arrojo e Drigo, J. S. Capitanio, R. Ramachandra, M. Ellisman,
    and M. Hetzer, “Identification of long-lived proteins in the mitochondria reveals
    increased stability of the electron transport chain,” <i>Developmental Cell</i>,
    vol. 56, no. 21. Elsevier, p. P2952–2965.e9, 2021.
  ista: Krishna S, Arrojo e Drigo R, Capitanio JS, Ramachandra R, Ellisman M, Hetzer
    M. 2021. Identification of long-lived proteins in the mitochondria reveals increased
    stability of the electron transport chain. Developmental Cell. 56(21), P2952–2965.e9.
  mla: Krishna, Shefali, et al. “Identification of Long-Lived Proteins in the Mitochondria
    Reveals Increased Stability of the Electron Transport Chain.” <i>Developmental
    Cell</i>, vol. 56, no. 21, Elsevier, 2021, p. P2952–2965.e9, doi:<a href="https://doi.org/10.1016/j.devcel.2021.10.008">10.1016/j.devcel.2021.10.008</a>.
  short: S. Krishna, R. Arrojo e Drigo, J.S. Capitanio, R. Ramachandra, M. Ellisman,
    M. Hetzer, Developmental Cell 56 (2021) P2952–2965.e9.
date_created: 2022-04-07T07:43:14Z
date_published: 2021-11-08T00:00:00Z
date_updated: 2025-12-15T10:01:56Z
day: '08'
department:
- _id: MaHe
doi: 10.1016/j.devcel.2021.10.008
extern: '1'
external_id:
  pmid:
  - '34715012'
fulldoi: https://doi.org/10.1016/j.devcel.2021.10.008
intvolume: '        56'
issue: '21'
keyword:
- Developmental Biology
- Cell Biology
- General Biochemistry
- Genetics and Molecular Biology
- Molecular Biology
language:
- iso: eng
month: '11'
oa_version: None
page: P2952-2965.e9
pmid: 1
publication: Developmental Cell
publication_identifier:
  issn:
  - 1534-5807
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Identification of long-lived proteins in the mitochondria reveals increased
  stability of the electron transport chain
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 56
year: '2021'
...