--- _id: '11052' abstract: - lang: eng text: In order to combat molecular damage, most cellular proteins undergo rapid turnover. We have previously identified large nuclear protein assemblies that can persist for years in post-mitotic tissues and are subject to age-related decline. Here, we report that mitochondria can be long lived in the mouse brain and reveal that specific mitochondrial proteins have half-lives longer than the average proteome. These mitochondrial long-lived proteins (mitoLLPs) are core components of the electron transport chain (ETC) and display increased longevity in respiratory supercomplexes. We find that COX7C, a mitoLLP that forms a stable contact site between complexes I and IV, is required for complex IV and supercomplex assembly. Remarkably, even upon depletion of COX7C transcripts, ETC function is maintained for days, effectively uncoupling mitochondrial function from ongoing transcription of its mitoLLPs. Our results suggest that modulating protein longevity within the ETC is critical for mitochondrial proteome maintenance and the robustness of mitochondrial function. article_processing_charge: No article_type: original author: - first_name: Shefali full_name: Krishna, Shefali last_name: Krishna - first_name: Rafael full_name: Arrojo e Drigo, Rafael last_name: Arrojo e Drigo - first_name: Juliana S. full_name: Capitanio, Juliana S. last_name: Capitanio - first_name: Ranjan full_name: Ramachandra, Ranjan last_name: Ramachandra - first_name: Mark full_name: Ellisman, Mark last_name: Ellisman - first_name: Martin W full_name: HETZER, Martin W id: 86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed last_name: HETZER orcid: 0000-0002-2111-992X citation: ama: Krishna S, Arrojo e Drigo R, Capitanio JS, Ramachandra R, Ellisman M, Hetzer M. Identification of long-lived proteins in the mitochondria reveals increased stability of the electron transport chain. Developmental Cell. 2021;56(21):P2952-2965.e9. doi:10.1016/j.devcel.2021.10.008 apa: Krishna, S., Arrojo e Drigo, R., Capitanio, J. S., Ramachandra, R., Ellisman, M., & Hetzer, M. (2021). Identification of long-lived proteins in the mitochondria reveals increased stability of the electron transport chain. Developmental Cell. Elsevier. https://doi.org/10.1016/j.devcel.2021.10.008 chicago: Krishna, Shefali, Rafael Arrojo e Drigo, Juliana S. Capitanio, Ranjan Ramachandra, Mark Ellisman, and Martin Hetzer. “Identification of Long-Lived Proteins in the Mitochondria Reveals Increased Stability of the Electron Transport Chain.” Developmental Cell. Elsevier, 2021. https://doi.org/10.1016/j.devcel.2021.10.008. ieee: S. Krishna, R. Arrojo e Drigo, J. S. Capitanio, R. Ramachandra, M. Ellisman, and M. Hetzer, “Identification of long-lived proteins in the mitochondria reveals increased stability of the electron transport chain,” Developmental Cell, vol. 56, no. 21. Elsevier, p. P2952–2965.e9, 2021. ista: Krishna S, Arrojo e Drigo R, Capitanio JS, Ramachandra R, Ellisman M, Hetzer M. 2021. Identification of long-lived proteins in the mitochondria reveals increased stability of the electron transport chain. Developmental Cell. 56(21), P2952–2965.e9. mla: Krishna, Shefali, et al. “Identification of Long-Lived Proteins in the Mitochondria Reveals Increased Stability of the Electron Transport Chain.” Developmental Cell, vol. 56, no. 21, Elsevier, 2021, p. P2952–2965.e9, doi:10.1016/j.devcel.2021.10.008. short: S. Krishna, R. Arrojo e Drigo, J.S. Capitanio, R. Ramachandra, M. Ellisman, M. Hetzer, Developmental Cell 56 (2021) P2952–2965.e9. date_created: 2022-04-07T07:43:14Z date_published: 2021-11-08T00:00:00Z date_updated: 2022-07-18T08:26:38Z day: '08' doi: 10.1016/j.devcel.2021.10.008 extern: '1' external_id: pmid: - '34715012' intvolume: ' 56' issue: '21' keyword: - Developmental Biology - Cell Biology - General Biochemistry - Genetics and Molecular Biology - Molecular Biology language: - iso: eng month: '11' oa_version: None page: P2952-2965.e9 pmid: 1 publication: Developmental Cell publication_identifier: issn: - 1534-5807 publication_status: published publisher: Elsevier quality_controlled: '1' scopus_import: '1' status: public title: Identification of long-lived proteins in the mitochondria reveals increased stability of the electron transport chain type: journal_article user_id: 72615eeb-f1f3-11ec-aa25-d4573ddc34fd volume: 56 year: '2021' ...