---
res:
  bibo_abstract:
  - Vascular dysfunctions are a common feature of multiple age-related diseases. However,
    modeling healthy and pathological aging of the human vasculature represents an
    unresolved experimental challenge. Here, we generated induced vascular endothelial
    cells (iVECs) and smooth muscle cells (iSMCs) by direct reprogramming of healthy
    human fibroblasts from donors of different ages and Hutchinson-Gilford Progeria
    Syndrome (HGPS) patients. iVECs induced from old donors revealed upregulation
    of GSTM1 and PALD1, genes linked to oxidative stress, inflammation and endothelial
    junction stability, as vascular aging markers. A functional assay performed on
    PALD1 KD VECs demonstrated a recovery in vascular permeability. We found that
    iSMCs from HGPS donors overexpressed bone morphogenetic protein (BMP)−4, which
    plays a key role in both vascular calcification and endothelial barrier damage
    observed in HGPS. Strikingly, BMP4 concentrations are higher in serum from HGPS
    vs. age-matched mice. Furthermore, targeting BMP4 with blocking antibody recovered
    the functionality of the vascular barrier in vitro, hence representing a potential
    future therapeutic strategy to limit cardiovascular dysfunction in HGPS. These
    results show that iVECs and iSMCs retain disease-related signatures, allowing
    modeling of vascular aging and HGPS in vitro.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Simone
      foaf_name: Bersini, Simone
      foaf_surname: Bersini
  - foaf_Person:
      foaf_givenName: Roberta
      foaf_name: Schulte, Roberta
      foaf_surname: Schulte
  - foaf_Person:
      foaf_givenName: Ling
      foaf_name: Huang, Ling
      foaf_surname: Huang
  - foaf_Person:
      foaf_givenName: Hannah
      foaf_name: Tsai, Hannah
      foaf_surname: Tsai
  - foaf_Person:
      foaf_givenName: Martin W
      foaf_name: HETZER, Martin W
      foaf_surname: HETZER
      foaf_workInfoHomepage: http://www.librecat.org/personId=86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed
    orcid: 0000-0002-2111-992X
  bibo_doi: 10.7554/elife.54383
  bibo_volume: 9
  dct_date: 2020^xs_gYear
  dct_isPartOf:
  - http://id.crossref.org/issn/2050-084X
  dct_language: eng
  dct_publisher: eLife Sciences Publications@
  dct_subject:
  - General Immunology and Microbiology
  - General Biochemistry
  - Genetics and Molecular Biology
  - General Medicine
  - General Neuroscience
  dct_title: Direct reprogramming of human smooth muscle and vascular endothelial
    cells reveals defects associated with aging and Hutchinson-Gilford progeria syndrome@
  fabio_hasPubmedId: '32896271'
...