---
res:
  bibo_abstract:
  - Aging of the circulatory system correlates with the pathogenesis of a large spectrum
    of diseases. However, it is largely unknown which factors drive the age-dependent
    or pathological decline of the vasculature and how vascular defects relate to
    tissue aging. The goal of the study is to design a multianalytical approach to
    identify how the cellular microenvironment (i.e., fibroblasts) and serum from
    healthy donors of different ages or Alzheimer disease (AD) patients can modulate
    the functionality of organ-specific vascular endothelial cells (VECs). Long-living
    human microvascular networks embedding VECs and fibroblasts from skin biopsies
    are generated. RNA-seq, secretome analyses, and microfluidic assays demonstrate
    that fibroblasts from young donors restore the functionality of aged endothelial
    cells, an effect also achieved by serum from young donors. New biomarkers of vascular
    aging are validated in human biopsies and it is shown that young serum induces
    angiopoietin-like-4, which can restore compromised vascular barriers. This strategy
    is then employed to characterize transcriptional/functional changes induced on
    the blood–brain barrier by AD serum, demonstrating the importance of PTP4A3 in
    the regulation of permeability. Features of vascular degeneration during aging
    and AD are recapitulated, and a tool to identify novel biomarkers that can be
    exploited to develop future therapeutics modulating vascular function is established.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Simone
      foaf_name: Bersini, Simone
      foaf_surname: Bersini
  - foaf_Person:
      foaf_givenName: Rafael
      foaf_name: Arrojo e Drigo, Rafael
      foaf_surname: Arrojo e Drigo
  - foaf_Person:
      foaf_givenName: Ling
      foaf_name: Huang, Ling
      foaf_surname: Huang
  - foaf_Person:
      foaf_givenName: Maxim N.
      foaf_name: Shokhirev, Maxim N.
      foaf_surname: Shokhirev
  - foaf_Person:
      foaf_givenName: Martin W
      foaf_name: HETZER, Martin W
      foaf_surname: HETZER
      foaf_workInfoHomepage: http://www.librecat.org/personId=86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed
    orcid: 0000-0002-2111-992X
  bibo_doi: 10.1002/adbi.202000044
  bibo_issue: '5'
  bibo_volume: 4
  dct_date: 2020^xs_gYear
  dct_isPartOf:
  - http://id.crossref.org/issn/2366-7478
  - http://id.crossref.org/issn/2366-7478
  dct_language: eng
  dct_publisher: Wiley@
  dct_subject:
  - General Biochemistry
  - Genetics and Molecular Biology
  - Biomedical Engineering
  - Biomaterials
  dct_title: Transcriptional and functional changes of the human microvasculature
    during physiological aging and Alzheimer disease@
  fabio_hasPubmedId: '32402127'
...