{"publication_status":"published","external_id":{"pmid":["25778917"]},"year":"2015","volume":208,"publisher":"Rockefeller University Press","day":"16","language":[{"iso":"eng"}],"date_updated":"2022-07-18T08:43:00Z","_id":"11075","publication":"Journal of Cell Biology","issue":"6","doi":"10.1083/jcb.201410047","page":"671-681","extern":"1","title":"The nucleoporin gp210/Nup210 controls muscle differentiation by regulating nuclear envelope/ER homeostasis","status":"public","month":"03","type":"journal_article","publication_identifier":{"issn":["0021-9525"],"eissn":["1540-8140"]},"article_processing_charge":"No","user_id":"72615eeb-f1f3-11ec-aa25-d4573ddc34fd","article_type":"original","date_created":"2022-04-07T07:49:10Z","oa_version":"Published Version","keyword":["Cell Biology"],"date_published":"2015-03-16T00:00:00Z","scopus_import":"1","quality_controlled":"1","pmid":1,"abstract":[{"text":"Previously, we identified the nucleoporin gp210/Nup210 as a critical regulator of muscle and neuronal differentiation, but how this nucleoporin exerts its function and whether it modulates nuclear pore complex (NPC) activity remain unknown. Here, we show that gp210/Nup210 mediates muscle cell differentiation in vitro via its conserved N-terminal domain that extends into the perinuclear space. Removal of the C-terminal domain, which partially mislocalizes gp210/Nup210 away from NPCs, efficiently rescues the differentiation defect caused by the knockdown of endogenous gp210/Nup210. Unexpectedly, a gp210/Nup210 mutant lacking the NPC-targeting transmembrane and C-terminal domains is sufficient for C2C12 myoblast differentiation. We demonstrate that the endoplasmic reticulum (ER) stress-specific caspase cascade is exacerbated during Nup210 depletion and that blocking ER stress-mediated apoptosis rescues differentiation of Nup210-deficient cells. Our results suggest that the role of gp210/Nup210 in cell differentiation is mediated by its large luminal domain, which can act independently of NPC association and appears to play a pivotal role in the maintenance of nuclear envelope/ER homeostasis.","lang":"eng"}],"author":[{"first_name":"J. Sebastian","full_name":"Gomez-Cavazos, J. Sebastian","last_name":"Gomez-Cavazos"},{"last_name":"HETZER","full_name":"HETZER, Martin W","orcid":"0000-0002-2111-992X","id":"86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed","first_name":"Martin W"}],"intvolume":"       208","citation":{"chicago":"Gomez-Cavazos, J. Sebastian, and Martin Hetzer. “The Nucleoporin Gp210/Nup210 Controls Muscle Differentiation by Regulating Nuclear Envelope/ER Homeostasis.” <i>Journal of Cell Biology</i>. Rockefeller University Press, 2015. <a href=\"https://doi.org/10.1083/jcb.201410047\">https://doi.org/10.1083/jcb.201410047</a>.","ieee":"J. S. Gomez-Cavazos and M. Hetzer, “The nucleoporin gp210/Nup210 controls muscle differentiation by regulating nuclear envelope/ER homeostasis,” <i>Journal of Cell Biology</i>, vol. 208, no. 6. Rockefeller University Press, pp. 671–681, 2015.","apa":"Gomez-Cavazos, J. S., &#38; Hetzer, M. (2015). The nucleoporin gp210/Nup210 controls muscle differentiation by regulating nuclear envelope/ER homeostasis. <i>Journal of Cell Biology</i>. Rockefeller University Press. <a href=\"https://doi.org/10.1083/jcb.201410047\">https://doi.org/10.1083/jcb.201410047</a>","mla":"Gomez-Cavazos, J. Sebastian, and Martin Hetzer. “The Nucleoporin Gp210/Nup210 Controls Muscle Differentiation by Regulating Nuclear Envelope/ER Homeostasis.” <i>Journal of Cell Biology</i>, vol. 208, no. 6, Rockefeller University Press, 2015, pp. 671–81, doi:<a href=\"https://doi.org/10.1083/jcb.201410047\">10.1083/jcb.201410047</a>.","ista":"Gomez-Cavazos JS, Hetzer M. 2015. The nucleoporin gp210/Nup210 controls muscle differentiation by regulating nuclear envelope/ER homeostasis. Journal of Cell Biology. 208(6), 671–681.","short":"J.S. Gomez-Cavazos, M. Hetzer, Journal of Cell Biology 208 (2015) 671–681.","ama":"Gomez-Cavazos JS, Hetzer M. The nucleoporin gp210/Nup210 controls muscle differentiation by regulating nuclear envelope/ER homeostasis. <i>Journal of Cell Biology</i>. 2015;208(6):671-681. doi:<a href=\"https://doi.org/10.1083/jcb.201410047\">10.1083/jcb.201410047</a>"}}