---
_id: '11075'
abstract:
- lang: eng
  text: Previously, we identified the nucleoporin gp210/Nup210 as a critical regulator
    of muscle and neuronal differentiation, but how this nucleoporin exerts its function
    and whether it modulates nuclear pore complex (NPC) activity remain unknown. Here,
    we show that gp210/Nup210 mediates muscle cell differentiation in vitro via its
    conserved N-terminal domain that extends into the perinuclear space. Removal of
    the C-terminal domain, which partially mislocalizes gp210/Nup210 away from NPCs,
    efficiently rescues the differentiation defect caused by the knockdown of endogenous
    gp210/Nup210. Unexpectedly, a gp210/Nup210 mutant lacking the NPC-targeting transmembrane
    and C-terminal domains is sufficient for C2C12 myoblast differentiation. We demonstrate
    that the endoplasmic reticulum (ER) stress-specific caspase cascade is exacerbated
    during Nup210 depletion and that blocking ER stress-mediated apoptosis rescues
    differentiation of Nup210-deficient cells. Our results suggest that the role of
    gp210/Nup210 in cell differentiation is mediated by its large luminal domain,
    which can act independently of NPC association and appears to play a pivotal role
    in the maintenance of nuclear envelope/ER homeostasis.
article_processing_charge: No
article_type: original
author:
- first_name: J. Sebastian
  full_name: Gomez-Cavazos, J. Sebastian
  last_name: Gomez-Cavazos
- first_name: Martin W
  full_name: HETZER, Martin W
  id: 86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed
  last_name: HETZER
  orcid: 0000-0002-2111-992X
citation:
  ama: Gomez-Cavazos JS, Hetzer M. The nucleoporin gp210/Nup210 controls muscle differentiation
    by regulating nuclear envelope/ER homeostasis. <i>Journal of Cell Biology</i>.
    2015;208(6):671-681. doi:<a href="https://doi.org/10.1083/jcb.201410047">10.1083/jcb.201410047</a>
  apa: Gomez-Cavazos, J. S., &#38; Hetzer, M. (2015). The nucleoporin gp210/Nup210
    controls muscle differentiation by regulating nuclear envelope/ER homeostasis.
    <i>Journal of Cell Biology</i>. Rockefeller University Press. <a href="https://doi.org/10.1083/jcb.201410047">https://doi.org/10.1083/jcb.201410047</a>
  chicago: Gomez-Cavazos, J. Sebastian, and Martin Hetzer. “The Nucleoporin Gp210/Nup210
    Controls Muscle Differentiation by Regulating Nuclear Envelope/ER Homeostasis.”
    <i>Journal of Cell Biology</i>. Rockefeller University Press, 2015. <a href="https://doi.org/10.1083/jcb.201410047">https://doi.org/10.1083/jcb.201410047</a>.
  ieee: J. S. Gomez-Cavazos and M. Hetzer, “The nucleoporin gp210/Nup210 controls
    muscle differentiation by regulating nuclear envelope/ER homeostasis,” <i>Journal
    of Cell Biology</i>, vol. 208, no. 6. Rockefeller University Press, pp. 671–681,
    2015.
  ista: Gomez-Cavazos JS, Hetzer M. 2015. The nucleoporin gp210/Nup210 controls muscle
    differentiation by regulating nuclear envelope/ER homeostasis. Journal of Cell
    Biology. 208(6), 671–681.
  mla: Gomez-Cavazos, J. Sebastian, and Martin Hetzer. “The Nucleoporin Gp210/Nup210
    Controls Muscle Differentiation by Regulating Nuclear Envelope/ER Homeostasis.”
    <i>Journal of Cell Biology</i>, vol. 208, no. 6, Rockefeller University Press,
    2015, pp. 671–81, doi:<a href="https://doi.org/10.1083/jcb.201410047">10.1083/jcb.201410047</a>.
  short: J.S. Gomez-Cavazos, M. Hetzer, Journal of Cell Biology 208 (2015) 671–681.
date_created: 2022-04-07T07:49:10Z
date_published: 2015-03-16T00:00:00Z
date_updated: 2024-10-14T11:22:26Z
day: '16'
doi: 10.1083/jcb.201410047
extern: '1'
external_id:
  pmid:
  - '25778917'
intvolume: '       208'
issue: '6'
keyword:
- Cell Biology
language:
- iso: eng
month: '03'
oa_version: Published Version
page: 671-681
pmid: 1
publication: Journal of Cell Biology
publication_identifier:
  eissn:
  - 1540-8140
  issn:
  - 0021-9525
publication_status: published
publisher: Rockefeller University Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: The nucleoporin gp210/Nup210 controls muscle differentiation by regulating
  nuclear envelope/ER homeostasis
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 208
year: '2015'
...