---
_id: '11082'
abstract:
- lang: eng
  text: The nuclear pore complex (NPC) plays a critical role in gene expression by
    mediating import of transcription regulators into the nucleus and export of RNA
    transcripts to the cytoplasm. Emerging evidence suggests that in addition to mediating
    transport, a subset of nucleoporins (Nups) engage in transcriptional activation
    and elongation at genomic loci that are not associated with NPCs. The underlying
    mechanism and regulation of Nup mobility on and off nuclear pores remain unclear.
    Here we show that Nup50 is a mobile Nup with a pronounced presence both at the
    NPC and in the nucleoplasm that can move between these different localizations.
    Strikingly, the dynamic behavior of Nup50 in both locations is dependent on active
    transcription by RNA polymerase II and requires the N-terminal half of the protein,
    which contains importin α– and Nup153-binding domains. However, Nup50 dynamics
    are independent of importin α, Nup153, and Nup98, even though the latter two proteins
    also exhibit transcription-dependent mobility. Of interest, depletion of Nup50
    from C2C12 myoblasts does not affect cell proliferation but inhibits differentiation
    into myotubes. Taken together, our results suggest a transport-independent role
    for Nup50 in chromatin biology that occurs away from the NPC.
article_processing_charge: No
article_type: original
author:
- first_name: Abigail L.
  full_name: Buchwalter, Abigail L.
  last_name: Buchwalter
- first_name: Yun
  full_name: Liang, Yun
  last_name: Liang
- first_name: Martin W
  full_name: HETZER, Martin W
  id: 86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed
  last_name: HETZER
  orcid: 0000-0002-2111-992X
citation:
  ama: Buchwalter AL, Liang Y, Hetzer M. Nup50 is required for cell differentiation
    and exhibits transcription-dependent dynamics. <i>Molecular Biology of the Cell</i>.
    2014;25(16):2472-2484. doi:<a href="https://doi.org/10.1091/mbc.e14-04-0865">10.1091/mbc.e14-04-0865</a>
  apa: Buchwalter, A. L., Liang, Y., &#38; Hetzer, M. (2014). Nup50 is required for
    cell differentiation and exhibits transcription-dependent dynamics. <i>Molecular
    Biology of the Cell</i>. American Society for Cell Biology. <a href="https://doi.org/10.1091/mbc.e14-04-0865">https://doi.org/10.1091/mbc.e14-04-0865</a>
  chicago: Buchwalter, Abigail L., Yun Liang, and Martin Hetzer. “Nup50 Is Required
    for Cell Differentiation and Exhibits Transcription-Dependent Dynamics.” <i>Molecular
    Biology of the Cell</i>. American Society for Cell Biology, 2014. <a href="https://doi.org/10.1091/mbc.e14-04-0865">https://doi.org/10.1091/mbc.e14-04-0865</a>.
  ieee: A. L. Buchwalter, Y. Liang, and M. Hetzer, “Nup50 is required for cell differentiation
    and exhibits transcription-dependent dynamics,” <i>Molecular Biology of the Cell</i>,
    vol. 25, no. 16. American Society for Cell Biology, pp. 2472–2484, 2014.
  ista: Buchwalter AL, Liang Y, Hetzer M. 2014. Nup50 is required for cell differentiation
    and exhibits transcription-dependent dynamics. Molecular Biology of the Cell.
    25(16), 2472–2484.
  mla: Buchwalter, Abigail L., et al. “Nup50 Is Required for Cell Differentiation
    and Exhibits Transcription-Dependent Dynamics.” <i>Molecular Biology of the Cell</i>,
    vol. 25, no. 16, American Society for Cell Biology, 2014, pp. 2472–84, doi:<a
    href="https://doi.org/10.1091/mbc.e14-04-0865">10.1091/mbc.e14-04-0865</a>.
  short: A.L. Buchwalter, Y. Liang, M. Hetzer, Molecular Biology of the Cell 25 (2014)
    2472–2484.
date_created: 2022-04-07T07:50:24Z
date_published: 2014-08-15T00:00:00Z
date_updated: 2024-10-14T11:23:34Z
day: '15'
doi: 10.1091/mbc.e14-04-0865
extern: '1'
intvolume: '        25'
issue: '16'
keyword:
- Cell Biology
- Molecular Biology
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1091/mbc.e14-04-0865
month: '08'
oa: 1
oa_version: Published Version
page: 2472-2484
publication: Molecular Biology of the Cell
publication_identifier:
  issn:
  - 1059-1524
  - 1939-4586
publication_status: published
publisher: American Society for Cell Biology
quality_controlled: '1'
scopus_import: '1'
status: public
title: Nup50 is required for cell differentiation and exhibits transcription-dependent
  dynamics
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 25
year: '2014'
...