{"pmid":1,"external_id":{"pmid":["18446240"]},"date_published":"2008-04-30T00:00:00Z","user_id":"72615eeb-f1f3-11ec-aa25-d4573ddc34fd","article_type":"original","oa":1,"quality_controlled":"1","language":[{"iso":"eng"}],"date_updated":"2022-07-18T08:56:36Z","publication_status":"published","extern":"1","publisher":"Public Library of Science","type":"journal_article","article_processing_charge":"No","article_number":"e2061","year":"2008","citation":{"mla":"Schulte, Roberta, et al. “Single Bead Affinity Detection (SINBAD) for the Analysis of Protein-Protein Interactions.” <i>PLoS ONE</i>, vol. 3, no. 4, e2061, Public Library of Science, 2008, doi:<a href=\"https://doi.org/10.1371/journal.pone.0002061\">10.1371/journal.pone.0002061</a>.","ieee":"R. Schulte, J. Talamas, C. Doucet, and M. Hetzer, “Single bead affinity detection (SINBAD) for the analysis of protein-protein interactions,” <i>PLoS ONE</i>, vol. 3, no. 4. Public Library of Science, 2008.","apa":"Schulte, R., Talamas, J., Doucet, C., &#38; Hetzer, M. (2008). Single bead affinity detection (SINBAD) for the analysis of protein-protein interactions. <i>PLoS ONE</i>. Public Library of Science. <a href=\"https://doi.org/10.1371/journal.pone.0002061\">https://doi.org/10.1371/journal.pone.0002061</a>","ama":"Schulte R, Talamas J, Doucet C, Hetzer M. Single bead affinity detection (SINBAD) for the analysis of protein-protein interactions. <i>PLoS ONE</i>. 2008;3(4). doi:<a href=\"https://doi.org/10.1371/journal.pone.0002061\">10.1371/journal.pone.0002061</a>","chicago":"Schulte, Roberta, Jessica Talamas, Christine Doucet, and Martin Hetzer. “Single Bead Affinity Detection (SINBAD) for the Analysis of Protein-Protein Interactions.” <i>PLoS ONE</i>. Public Library of Science, 2008. <a href=\"https://doi.org/10.1371/journal.pone.0002061\">https://doi.org/10.1371/journal.pone.0002061</a>.","ista":"Schulte R, Talamas J, Doucet C, Hetzer M. 2008. Single bead affinity detection (SINBAD) for the analysis of protein-protein interactions. PLoS ONE. 3(4), e2061.","short":"R. Schulte, J. Talamas, C. Doucet, M. Hetzer, PLoS ONE 3 (2008)."},"issue":"4","date_created":"2022-04-07T07:55:57Z","author":[{"full_name":"Schulte, Roberta","last_name":"Schulte","first_name":"Roberta"},{"full_name":"Talamas, Jessica","last_name":"Talamas","first_name":"Jessica"},{"full_name":"Doucet, Christine","last_name":"Doucet","first_name":"Christine"},{"orcid":"0000-0002-2111-992X","last_name":"HETZER","full_name":"HETZER, Martin W","id":"86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed","first_name":"Martin W"}],"scopus_import":"1","intvolume":"         3","day":"30","title":"Single bead affinity detection (SINBAD) for the analysis of protein-protein interactions","main_file_link":[{"open_access":"1","url":" https://doi.org/10.1371/journal.pone.0002061"}],"abstract":[{"text":"We present a miniaturized pull-down method for the detection of protein-protein interactions using standard affinity chromatography reagents. Binding events between different proteins, which are color-coded with quantum dots (QDs), are visualized on single affinity chromatography beads by fluorescence microscopy. The use of QDs for single molecule detection allows the simultaneous analysis of multiple protein-protein binding events and reduces the amount of time and material needed to perform a pull-down experiment.","lang":"eng"}],"doi":"10.1371/journal.pone.0002061","publication_identifier":{"issn":["1932-6203"]},"month":"04","keyword":["Multidisciplinary"],"publication":"PLoS ONE","volume":3,"oa_version":"Published Version","status":"public","_id":"11114"}