---
_id: '11114'
abstract:
- lang: eng
text: We present a miniaturized pull-down method for the detection of protein-protein
interactions using standard affinity chromatography reagents. Binding events between
different proteins, which are color-coded with quantum dots (QDs), are visualized
on single affinity chromatography beads by fluorescence microscopy. The use of
QDs for single molecule detection allows the simultaneous analysis of multiple
protein-protein binding events and reduces the amount of time and material needed
to perform a pull-down experiment.
article_number: e2061
article_processing_charge: No
article_type: original
author:
- first_name: Roberta
full_name: Schulte, Roberta
last_name: Schulte
- first_name: Jessica
full_name: Talamas, Jessica
last_name: Talamas
- first_name: Christine
full_name: Doucet, Christine
last_name: Doucet
- first_name: Martin W
full_name: HETZER, Martin W
id: 86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed
last_name: HETZER
orcid: 0000-0002-2111-992X
citation:
ama: Schulte R, Talamas J, Doucet C, Hetzer M. Single bead affinity detection (SINBAD)
for the analysis of protein-protein interactions. PLoS ONE. 2008;3(4).
doi:10.1371/journal.pone.0002061
apa: Schulte, R., Talamas, J., Doucet, C., & Hetzer, M. (2008). Single bead
affinity detection (SINBAD) for the analysis of protein-protein interactions.
PLoS ONE. Public Library of Science. https://doi.org/10.1371/journal.pone.0002061
chicago: Schulte, Roberta, Jessica Talamas, Christine Doucet, and Martin Hetzer.
“Single Bead Affinity Detection (SINBAD) for the Analysis of Protein-Protein Interactions.”
PLoS ONE. Public Library of Science, 2008. https://doi.org/10.1371/journal.pone.0002061.
ieee: R. Schulte, J. Talamas, C. Doucet, and M. Hetzer, “Single bead affinity detection
(SINBAD) for the analysis of protein-protein interactions,” PLoS ONE, vol.
3, no. 4. Public Library of Science, 2008.
ista: Schulte R, Talamas J, Doucet C, Hetzer M. 2008. Single bead affinity detection
(SINBAD) for the analysis of protein-protein interactions. PLoS ONE. 3(4), e2061.
mla: Schulte, Roberta, et al. “Single Bead Affinity Detection (SINBAD) for the Analysis
of Protein-Protein Interactions.” PLoS ONE, vol. 3, no. 4, e2061, Public
Library of Science, 2008, doi:10.1371/journal.pone.0002061.
short: R. Schulte, J. Talamas, C. Doucet, M. Hetzer, PLoS ONE 3 (2008).
date_created: 2022-04-07T07:55:57Z
date_published: 2008-04-30T00:00:00Z
date_updated: 2022-07-18T08:56:36Z
day: '30'
doi: 10.1371/journal.pone.0002061
extern: '1'
external_id:
pmid:
- '18446240'
intvolume: ' 3'
issue: '4'
keyword:
- Multidisciplinary
language:
- iso: eng
main_file_link:
- open_access: '1'
url: ' https://doi.org/10.1371/journal.pone.0002061'
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
publication: PLoS ONE
publication_identifier:
issn:
- 1932-6203
publication_status: published
publisher: Public Library of Science
quality_controlled: '1'
scopus_import: '1'
status: public
title: Single bead affinity detection (SINBAD) for the analysis of protein-protein
interactions
type: journal_article
user_id: 72615eeb-f1f3-11ec-aa25-d4573ddc34fd
volume: 3
year: '2008'
...