Nuclear envelope formation by chromatin-mediated reorganization of the endoplasmic reticulum Anderson, Daniel J. HETZER, Martin W ; https://orcid.org/0000-0002-2111-992X Cell Biology The formation of the nuclear envelope (NE) around chromatin is a major membrane-remodelling event that occurs during cell division of metazoa. It is unclear whether the nuclear membrane reforms by the fusion of NE fragments or if it re-emerges from an intact tubular network of the endoplasmic reticulum (ER). Here, we show that NE formation and expansion requires a tubular ER network and occurs efficiently in the presence of the membrane fusion inhibitor GTPγS. Chromatin recruitment of membranes, which is initiated by tubule-end binding, followed by the formation, expansion and sealing of flat membrane sheets, is mediated by DNA-binding proteins residing in the ER. Thus, chromatin plays an active role in reshaping of the ER during NE formation. Springer Nature 2007 info:eu-repo/semantics/article doc-type:article text http://purl.org/coar/resource_type/c_2df8fbb1 https://research-explorer.ista.ac.at/record/11115 Anderson DJ, Hetzer M. Nuclear envelope formation by chromatin-mediated reorganization of the endoplasmic reticulum. <i>Nature Cell Biology</i>. 2007;9(10):1160-1166. doi:<a href="https://doi.org/10.1038/ncb1636">10.1038/ncb1636</a> eng info:eu-repo/semantics/altIdentifier/doi/10.1038/ncb1636 info:eu-repo/semantics/altIdentifier/issn/1465-7392 info:eu-repo/semantics/altIdentifier/e-issn/1476-4679 info:eu-repo/semantics/altIdentifier/pmid/17828249 info:eu-repo/semantics/closedAccess