Nuclear envelope formation by chromatin-mediated reorganization of the endoplasmic reticulum

article Nuclear envelope formation by chromatin-mediated reorganization of the endoplasmic reticulum published yes Daniel J. Anderson author Martin W HETZER author 86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed0000-0002-2111-992X The formation of the nuclear envelope (NE) around chromatin is a major membrane-remodelling event that occurs during cell division of metazoa. It is unclear whether the nuclear membrane reforms by the fusion of NE fragments or if it re-emerges from an intact tubular network of the endoplasmic reticulum (ER). Here, we show that NE formation and expansion requires a tubular ER network and occurs efficiently in the presence of the membrane fusion inhibitor GTPγS. Chromatin recruitment of membranes, which is initiated by tubule-end binding, followed by the formation, expansion and sealing of flat membrane sheets, is mediated by DNA-binding proteins residing in the ER. Thus, chromatin plays an active role in reshaping of the ER during NE formation. Springer Nature2007 eng Cell Biology Nature Cell Biology 1465-7392 1476-4679 1782824910.1038/ncb1636 9101160-1166 yes Anderson DJ, Hetzer M. Nuclear envelope formation by chromatin-mediated reorganization of the endoplasmic reticulum. Nature Cell Biology. 2007;9(10):1160-1166. doi:<a href="https://doi.org/10.1038/ncb1636">10.1038/ncb1636</a> D.J. Anderson, M. Hetzer, Nature Cell Biology 9 (2007) 1160–1166. Anderson DJ, Hetzer M. 2007. Nuclear envelope formation by chromatin-mediated reorganization of the endoplasmic reticulum. Nature Cell Biology. 9(10), 1160–1166. Anderson, Daniel J., and Martin Hetzer. “Nuclear Envelope Formation by Chromatin-Mediated Reorganization of the Endoplasmic Reticulum.” Nature Cell Biology. Springer Nature, 2007. <a href="https://doi.org/10.1038/ncb1636">https://doi.org/10.1038/ncb1636</a>. Anderson, D. J., & Hetzer, M. (2007). Nuclear envelope formation by chromatin-mediated reorganization of the endoplasmic reticulum. Nature Cell Biology. Springer Nature. <a href="https://doi.org/10.1038/ncb1636">https://doi.org/10.1038/ncb1636</a> Anderson, Daniel J., and Martin Hetzer. “Nuclear Envelope Formation by Chromatin-Mediated Reorganization of the Endoplasmic Reticulum.” Nature Cell Biology, vol. 9, no. 10, Springer Nature, 2007, pp. 1160–66, doi:<a href="https://doi.org/10.1038/ncb1636">10.1038/ncb1636</a>. D. J. Anderson and M. Hetzer, “Nuclear envelope formation by chromatin-mediated reorganization of the endoplasmic reticulum,” Nature Cell Biology, vol. 9, no. 10. Springer Nature, pp. 1160–1166, 2007. 111152022-04-07T07:56:04Z2024-10-14T11:30:08Z