---
_id: '11122'
abstract:
- lang: eng
  text: Nuclear pore complexes (NPCs) are large multiprotein assemblies that allow
    traffic between the cytoplasm and the nucleus. During mitosis in higher eukaryotes,
    the Nuclear Envelope (NE) breaks down and NPCs disassemble. How NPCs reassemble
    and incorporate into the NE upon mitotic exit is poorly understood. We demonstrate
    a function for the conserved Nup107-160 complex in this process. Partial in vivo
    depletion of Nup133 or Nup107 via RNAi in HeLa cells resulted in reduced levels
    of multiple nucleoporins and decreased NPC density in the NE. Immunodepletion
    of the entire Nup107-160 complex from in vitro nuclear assembly reactions produced
    nuclei with a continuous NE but no NPCs. This phenotype was reversible only if
    Nup107-160 complex was readded before closed NE formation. Depletion also prevented
    association of FG-repeat nucleoporins with chromatin. We propose a stepwise model
    in which postmitotic NPC assembly initiates on chromatin via early recruitment
    of the Nup107-160 complex.
article_processing_charge: No
article_type: original
author:
- first_name: Tobias C.
  full_name: Walther, Tobias C.
  last_name: Walther
- first_name: Annabelle
  full_name: Alves, Annabelle
  last_name: Alves
- first_name: Helen
  full_name: Pickersgill, Helen
  last_name: Pickersgill
- first_name: Isabelle
  full_name: Loı̈odice, Isabelle
  last_name: Loı̈odice
- first_name: Martin W
  full_name: HETZER, Martin W
  id: 86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed
  last_name: HETZER
  orcid: 0000-0002-2111-992X
- first_name: Vincent
  full_name: Galy, Vincent
  last_name: Galy
- first_name: Bastian B.
  full_name: Hülsmann, Bastian B.
  last_name: Hülsmann
- first_name: Thomas
  full_name: Köcher, Thomas
  last_name: Köcher
- first_name: Matthias
  full_name: Wilm, Matthias
  last_name: Wilm
- first_name: Terry
  full_name: Allen, Terry
  last_name: Allen
- first_name: Iain W.
  full_name: Mattaj, Iain W.
  last_name: Mattaj
- first_name: Valérie
  full_name: Doye, Valérie
  last_name: Doye
citation:
  ama: Walther TC, Alves A, Pickersgill H, et al. The conserved Nup107-160 complex
    is critical for nuclear pore complex assembly. <i>Cell</i>. 2003;113(2):195-206.
    doi:<a href="https://doi.org/10.1016/s0092-8674(03)00235-6">10.1016/s0092-8674(03)00235-6</a>
  apa: Walther, T. C., Alves, A., Pickersgill, H., Loı̈odice, I., Hetzer, M., Galy,
    V., … Doye, V. (2003). The conserved Nup107-160 complex is critical for nuclear
    pore complex assembly. <i>Cell</i>. Elsevier. <a href="https://doi.org/10.1016/s0092-8674(03)00235-6">https://doi.org/10.1016/s0092-8674(03)00235-6</a>
  chicago: Walther, Tobias C., Annabelle Alves, Helen Pickersgill, Isabelle Loı̈odice,
    Martin Hetzer, Vincent Galy, Bastian B. Hülsmann, et al. “The Conserved Nup107-160
    Complex Is Critical for Nuclear Pore Complex Assembly.” <i>Cell</i>. Elsevier,
    2003. <a href="https://doi.org/10.1016/s0092-8674(03)00235-6">https://doi.org/10.1016/s0092-8674(03)00235-6</a>.
  ieee: T. C. Walther <i>et al.</i>, “The conserved Nup107-160 complex is critical
    for nuclear pore complex assembly,” <i>Cell</i>, vol. 113, no. 2. Elsevier, pp.
    195–206, 2003.
  ista: Walther TC, Alves A, Pickersgill H, Loı̈odice I, Hetzer M, Galy V, Hülsmann
    BB, Köcher T, Wilm M, Allen T, Mattaj IW, Doye V. 2003. The conserved Nup107-160
    complex is critical for nuclear pore complex assembly. Cell. 113(2), 195–206.
  mla: Walther, Tobias C., et al. “The Conserved Nup107-160 Complex Is Critical for
    Nuclear Pore Complex Assembly.” <i>Cell</i>, vol. 113, no. 2, Elsevier, 2003,
    pp. 195–206, doi:<a href="https://doi.org/10.1016/s0092-8674(03)00235-6">10.1016/s0092-8674(03)00235-6</a>.
  short: T.C. Walther, A. Alves, H. Pickersgill, I. Loı̈odice, M. Hetzer, V. Galy,
    B.B. Hülsmann, T. Köcher, M. Wilm, T. Allen, I.W. Mattaj, V. Doye, Cell 113 (2003)
    195–206.
date_created: 2022-04-07T07:57:10Z
date_published: 2003-04-17T00:00:00Z
date_updated: 2022-07-18T08:57:42Z
day: '17'
doi: 10.1016/s0092-8674(03)00235-6
extern: '1'
external_id:
  pmid:
  - '12705868'
intvolume: '       113'
issue: '2'
keyword:
- General Biochemistry
- Genetics and Molecular Biology
language:
- iso: eng
month: '04'
oa_version: Published Version
page: 195-206
pmid: 1
publication: Cell
publication_identifier:
  issn:
  - 0092-8674
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: The conserved Nup107-160 complex is critical for nuclear pore complex assembly
type: journal_article
user_id: 72615eeb-f1f3-11ec-aa25-d4573ddc34fd
volume: 113
year: '2003'
...