Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assembly HETZER, Martin W ; https://orcid.org/0000-0002-2111-992X Meyer, Hemmo H. Walther, Tobias C. Bilbao-Cortes, Daniel Warren, Graham Mattaj, Iain W. Cell Biology Although nuclear envelope (NE) assembly is known to require the GTPase Ran, the membrane fusion machinery involved is uncharacterized. NE assembly involves formation of a reticular network on chromatin, fusion of this network into a closed NE and subsequent expansion. Here we show that p97, an AAA-ATPase previously implicated in fusion of Golgi and transitional endoplasmic reticulum (ER) membranes together with the adaptor p47, has two discrete functions in NE assembly. Formation of a closed NE requires the p97–Ufd1–Npl4 complex, not previously implicated in membrane fusion. Subsequent NE growth involves a p97–p47 complex. This study provides the first insights into the molecular mechanisms and specificity of fusion events involved in NE formation. Springer Nature 2001 info:eu-repo/semantics/article doc-type:article text http://purl.org/coar/resource_type/c_2df8fbb1 https://research-explorer.ista.ac.at/record/11125 Hetzer M, Meyer HH, Walther TC, Bilbao-Cortes D, Warren G, Mattaj IW. Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assembly. <i>Nature Cell Biology</i>. 2001;3(12):1086-1091. doi:<a href="https://doi.org/10.1038/ncb1201-1086">10.1038/ncb1201-1086</a> eng info:eu-repo/semantics/altIdentifier/doi/10.1038/ncb1201-1086 info:eu-repo/semantics/altIdentifier/issn/1465-7392 info:eu-repo/semantics/altIdentifier/e-issn/1476-4679 info:eu-repo/semantics/altIdentifier/pmid/11781570 info:eu-repo/semantics/closedAccess