---
_id: '11125'
abstract:
- lang: eng
  text: Although nuclear envelope (NE) assembly is known to require the GTPase Ran,
    the membrane fusion machinery involved is uncharacterized. NE assembly involves
    formation of a reticular network on chromatin, fusion of this network into a closed
    NE and subsequent expansion. Here we show that p97, an AAA-ATPase previously implicated
    in fusion of Golgi and transitional endoplasmic reticulum (ER) membranes together
    with the adaptor p47, has two discrete functions in NE assembly. Formation of
    a closed NE requires the p97–Ufd1–Npl4 complex, not previously implicated in membrane
    fusion. Subsequent NE growth involves a p97–p47 complex. This study provides the
    first insights into the molecular mechanisms and specificity of fusion events
    involved in NE formation.
article_processing_charge: No
article_type: original
author:
- first_name: Martin W
  full_name: HETZER, Martin W
  id: 86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed
  last_name: HETZER
  orcid: 0000-0002-2111-992X
- first_name: Hemmo H.
  full_name: Meyer, Hemmo H.
  last_name: Meyer
- first_name: Tobias C.
  full_name: Walther, Tobias C.
  last_name: Walther
- first_name: Daniel
  full_name: Bilbao-Cortes, Daniel
  last_name: Bilbao-Cortes
- first_name: Graham
  full_name: Warren, Graham
  last_name: Warren
- first_name: Iain W.
  full_name: Mattaj, Iain W.
  last_name: Mattaj
citation:
  ama: Hetzer M, Meyer HH, Walther TC, Bilbao-Cortes D, Warren G, Mattaj IW. Distinct
    AAA-ATPase p97 complexes function in discrete steps of nuclear assembly. <i>Nature
    Cell Biology</i>. 2001;3(12):1086-1091. doi:<a href="https://doi.org/10.1038/ncb1201-1086">10.1038/ncb1201-1086</a>
  apa: Hetzer, M., Meyer, H. H., Walther, T. C., Bilbao-Cortes, D., Warren, G., &#38;
    Mattaj, I. W. (2001). Distinct AAA-ATPase p97 complexes function in discrete steps
    of nuclear assembly. <i>Nature Cell Biology</i>. Springer Nature. <a href="https://doi.org/10.1038/ncb1201-1086">https://doi.org/10.1038/ncb1201-1086</a>
  chicago: Hetzer, Martin, Hemmo H. Meyer, Tobias C. Walther, Daniel Bilbao-Cortes,
    Graham Warren, and Iain W. Mattaj. “Distinct AAA-ATPase P97 Complexes Function
    in Discrete Steps of Nuclear Assembly.” <i>Nature Cell Biology</i>. Springer Nature,
    2001. <a href="https://doi.org/10.1038/ncb1201-1086">https://doi.org/10.1038/ncb1201-1086</a>.
  ieee: M. Hetzer, H. H. Meyer, T. C. Walther, D. Bilbao-Cortes, G. Warren, and I.
    W. Mattaj, “Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear
    assembly,” <i>Nature Cell Biology</i>, vol. 3, no. 12. Springer Nature, pp. 1086–1091,
    2001.
  ista: Hetzer M, Meyer HH, Walther TC, Bilbao-Cortes D, Warren G, Mattaj IW. 2001.
    Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assembly.
    Nature Cell Biology. 3(12), 1086–1091.
  mla: Hetzer, Martin, et al. “Distinct AAA-ATPase P97 Complexes Function in Discrete
    Steps of Nuclear Assembly.” <i>Nature Cell Biology</i>, vol. 3, no. 12, Springer
    Nature, 2001, pp. 1086–91, doi:<a href="https://doi.org/10.1038/ncb1201-1086">10.1038/ncb1201-1086</a>.
  short: M. Hetzer, H.H. Meyer, T.C. Walther, D. Bilbao-Cortes, G. Warren, I.W. Mattaj,
    Nature Cell Biology 3 (2001) 1086–1091.
date_created: 2022-04-07T07:57:42Z
date_published: 2001-11-02T00:00:00Z
date_updated: 2022-07-18T08:58:07Z
day: '02'
doi: 10.1038/ncb1201-1086
extern: '1'
external_id:
  pmid:
  - '11781570'
intvolume: '         3'
issue: '12'
keyword:
- Cell Biology
language:
- iso: eng
month: '11'
oa_version: None
page: 1086-1091
pmid: 1
publication: Nature Cell Biology
publication_identifier:
  eissn:
  - 1476-4679
  issn:
  - 1465-7392
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assembly
type: journal_article
user_id: 72615eeb-f1f3-11ec-aa25-d4573ddc34fd
volume: 3
year: '2001'
...