--- _id: '11127' abstract: - lang: eng text: Nuclear formation in Xenopus egg extracts requires cytosol and is inhibited by GTPγS, indicating a requirement for GTPase activity. Nuclear envelope (NE) vesicle fusion is extensively inhibited by GTPγS and two mutant forms of the Ran GTPase, Q69L and T24N. Depletion of either Ran or RCC1, the exchange factor for Ran, from the assembly reaction also inhibits this step of NE formation. Ran depletion can be complemented by the addition of Ran loaded with either GTP or GDP but not with GTPγS. RCC1 depletion is only complemented by RCC1 itself or by RanGTP. Thus, generation of RanGTP by RCC1 and GTP hydrolysis by Ran are both required for the extensive membrane fusion events that lead to NE formation. article_processing_charge: No article_type: original author: - first_name: Martin W full_name: HETZER, Martin W id: 86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed last_name: HETZER orcid: 0000-0002-2111-992X - first_name: Daniel full_name: Bilbao-Cortés, Daniel last_name: Bilbao-Cortés - first_name: Tobias C full_name: Walther, Tobias C last_name: Walther - first_name: Oliver J full_name: Gruss, Oliver J last_name: Gruss - first_name: Iain W full_name: Mattaj, Iain W last_name: Mattaj citation: ama: Hetzer M, Bilbao-Cortés D, Walther TC, Gruss OJ, Mattaj IW. GTP hydrolysis by Ran is required for nuclear envelope assembly. Molecular Cell. 2000;5(6):1013-1024. doi:10.1016/s1097-2765(00)80266-x apa: Hetzer, M., Bilbao-Cortés, D., Walther, T. C., Gruss, O. J., & Mattaj, I. W. (2000). GTP hydrolysis by Ran is required for nuclear envelope assembly. Molecular Cell. Elsevier. https://doi.org/10.1016/s1097-2765(00)80266-x chicago: Hetzer, Martin, Daniel Bilbao-Cortés, Tobias C Walther, Oliver J Gruss, and Iain W Mattaj. “GTP Hydrolysis by Ran Is Required for Nuclear Envelope Assembly.” Molecular Cell. Elsevier, 2000. https://doi.org/10.1016/s1097-2765(00)80266-x. ieee: M. Hetzer, D. Bilbao-Cortés, T. C. Walther, O. J. Gruss, and I. W. Mattaj, “GTP hydrolysis by Ran is required for nuclear envelope assembly,” Molecular Cell, vol. 5, no. 6. Elsevier, pp. 1013–1024, 2000. ista: Hetzer M, Bilbao-Cortés D, Walther TC, Gruss OJ, Mattaj IW. 2000. GTP hydrolysis by Ran is required for nuclear envelope assembly. Molecular Cell. 5(6), 1013–1024. mla: Hetzer, Martin, et al. “GTP Hydrolysis by Ran Is Required for Nuclear Envelope Assembly.” Molecular Cell, vol. 5, no. 6, Elsevier, 2000, pp. 1013–24, doi:10.1016/s1097-2765(00)80266-x. short: M. Hetzer, D. Bilbao-Cortés, T.C. Walther, O.J. Gruss, I.W. Mattaj, Molecular Cell 5 (2000) 1013–1024. date_created: 2022-04-07T07:57:59Z date_published: 2000-06-01T00:00:00Z date_updated: 2022-07-18T08:58:31Z day: '01' doi: 10.1016/s1097-2765(00)80266-x extern: '1' external_id: pmid: - '10911995' intvolume: ' 5' issue: '6' keyword: - Cell Biology - Molecular Biology language: - iso: eng main_file_link: - open_access: '1' url: https://doi.org/10.1016/S1097-2765(00)80266-X month: '06' oa: 1 oa_version: Published Version page: 1013-1024 pmid: 1 publication: Molecular Cell publication_identifier: issn: - 1097-2765 publication_status: published publisher: Elsevier quality_controlled: '1' scopus_import: '1' status: public title: GTP hydrolysis by Ran is required for nuclear envelope assembly type: journal_article user_id: 72615eeb-f1f3-11ec-aa25-d4573ddc34fd volume: 5 year: '2000' ...