Phase-locked inhibition, but not excitation, underlies hippocampal ripple oscillations in awake mice in vivo

article Phase-locked inhibition, but not excitation, underlies hippocampal ripple oscillations in awake mice in vivo published yes Jian Gan author 3614E438-F248-11E8-B48F-1D18A9856A87 Shih-Ming Weng author 2F9C5AC8-F248-11E8-B48F-1D18A9856A87 Alejandro Pernia-Andrade author 36963E98-F248-11E8-B48F-1D18A9856A87 Jozsef L Csicsvari author 3FA14672-F248-11E8-B48F-1D18A9856A870000-0002-5193-4036 Peter M Jonas author 353C1B58-F248-11E8-B48F-1D18A9856A870000-0001-5001-4804 PeJo department JoCs department Mechanisms of transmitter release at GABAergic synapses project Nanophysiology of fast-spiking, parvalbumin-expressing GABAergic interneurons project Sharp wave-ripple (SWR) oscillations play a key role in memory consolidation during non-rapid eye movement sleep, immobility, and consummatory behavior. However, whether temporally modulated synaptic excitation or inhibition underlies the ripples is controversial. To address this question, we performed simultaneous recordings of excitatory and inhibitory postsynaptic currents (EPSCs and IPSCs) and local field potentials (LFPs) in the CA1 region of awake mice in vivo. During SWRs, inhibition dominated over excitation, with a peak conductance ratio of 4.1 ± 0.5. Furthermore, the amplitude of SWR-associated IPSCs was positively correlated with SWR magnitude, whereas that of EPSCs was not. Finally, phase analysis indicated that IPSCs were phase-locked to individual ripple cycles, whereas EPSCs were uniformly distributed in phase space. Optogenetic inhibition indicated that PV+ interneurons provided a major contribution to SWR-associated IPSCs. Thus, phasic inhibition, but not excitation, shapes SWR oscillations in the hippocampal CA1 region in vivo. https://research-explorer.ista.ac.at/download/1118/4719/IST-2017-752-v1+1_1-s2.0-S0896627316309606-main.pdf application/pdfno Elsevier2017 eng Neuron 00039642820001010.1016/j.neuron.2016.12.018 932308 - 314 Gan, Jian, Shih-Ming Weng, Alejandro Pernia-Andrade, Jozsef L Csicsvari, and Peter M Jonas. “Phase-Locked Inhibition, but Not Excitation, Underlies Hippocampal Ripple Oscillations in Awake Mice in Vivo.” Neuron. Elsevier, 2017. <a href="https://doi.org/10.1016/j.neuron.2016.12.018">https://doi.org/10.1016/j.neuron.2016.12.018</a>. Gan, J., Weng, S.-M., Pernia-Andrade, A., Csicsvari, J. L., & Jonas, P. M. (2017). Phase-locked inhibition, but not excitation, underlies hippocampal ripple oscillations in awake mice in vivo. Neuron. Elsevier. <a href="https://doi.org/10.1016/j.neuron.2016.12.018">https://doi.org/10.1016/j.neuron.2016.12.018</a> J. Gan, S.-M. Weng, A. Pernia-Andrade, J.L. Csicsvari, P.M. Jonas, Neuron 93 (2017) 308–314. Gan, Jian, et al. “Phase-Locked Inhibition, but Not Excitation, Underlies Hippocampal Ripple Oscillations in Awake Mice in Vivo.” Neuron, vol. 93, no. 2, Elsevier, 2017, pp. 308–14, doi:<a href="https://doi.org/10.1016/j.neuron.2016.12.018">10.1016/j.neuron.2016.12.018</a>. Gan J, Weng S-M, Pernia-Andrade A, Csicsvari JL, Jonas PM. Phase-locked inhibition, but not excitation, underlies hippocampal ripple oscillations in awake mice in vivo. Neuron. 2017;93(2):308-314. doi:<a href="https://doi.org/10.1016/j.neuron.2016.12.018">10.1016/j.neuron.2016.12.018</a> Gan J, Weng S-M, Pernia-Andrade A, Csicsvari JL, Jonas PM. 2017. Phase-locked inhibition, but not excitation, underlies hippocampal ripple oscillations in awake mice in vivo. Neuron. 93(2), 308–314. J. Gan, S.-M. Weng, A. Pernia-Andrade, J. L. Csicsvari, and P. M. Jonas, “Phase-locked inhibition, but not excitation, underlies hippocampal ripple oscillations in awake mice in vivo,” Neuron, vol. 93, no. 2. Elsevier, pp. 308–314, 2017. 11182018-12-11T11:50:15Z2025-04-15T07:20:01Z