--- res: bibo_abstract: - The generation of a correctly-sized cerebral cortex with all-embracing neuronal and glial cell-type diversity critically depends on faithful radial glial progenitor (RGP) cell proliferation/differentiation programs. Temporal RGP lineage progression is regulated by Polycomb Repressive Complex 2 (PRC2) and loss of PRC2 activity results in severe neurogenesis defects and microcephaly. How PRC2-dependent gene expression instructs RGP lineage progression is unknown. Here we utilize Mosaic Analysis with Double Markers (MADM)-based single cell technology and demonstrate that PRC2 is not cell-autonomously required in neurogenic RGPs but rather acts at the global tissue-wide level. Conversely, cortical astrocyte production and maturation is cell-autonomously controlled by PRC2-dependent transcriptional regulation. We thus reveal highly distinct and sequential PRC2 functions in RGP lineage progression that are dependent on complex interplays between intrinsic and tissue-wide properties. In a broader context our results imply a critical role for the genetic and cellular niche environment in neural stem cell behavior.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Nicole foaf_name: Amberg, Nicole foaf_surname: Amberg foaf_workInfoHomepage: http://www.librecat.org/personId=4CD6AAC6-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-3183-8207 - foaf_Person: foaf_givenName: Florian foaf_name: Pauler, Florian foaf_surname: Pauler foaf_workInfoHomepage: http://www.librecat.org/personId=48EA0138-F248-11E8-B48F-1D18A9856A87 - foaf_Person: foaf_givenName: Carmen foaf_name: Streicher, Carmen foaf_surname: Streicher foaf_workInfoHomepage: http://www.librecat.org/personId=36BCB99C-F248-11E8-B48F-1D18A9856A87 - foaf_Person: foaf_givenName: Simon foaf_name: Hippenmeyer, Simon foaf_surname: Hippenmeyer foaf_workInfoHomepage: http://www.librecat.org/personId=37B36620-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0003-2279-1061 bibo_doi: 10.1126/sciadv.abq1263 bibo_issue: '44' bibo_volume: 8 dct_date: 2022^xs_gYear dct_isPartOf: - http://id.crossref.org/issn/2375-2548 dct_language: eng dct_publisher: American Association for the Advancement of Science@ dct_title: Tissue-wide genetic and cellular landscape shapes the execution of sequential PRC2 functions in neural stem cell lineage progression@ ...