---
res:
  bibo_abstract:
  - "AMPA receptors (AMPARs) mediate fast excitatory neurotransmission and their role
    is\r\nimplicated in complex processes such as learning and memory and various
    neurological\r\ndiseases. These receptors are composed of different subunits and
    the subunit composition can\r\naffect channel properties, receptor trafficking
    and interaction with other associated proteins.\r\nUsing the high sensitivity
    SDS-digested freeze-fracture replica labeling (SDS-FRL) for\r\nelectron microscopy
    I investigated the number, density, and localization of AMPAR subunits,\r\nGluA1,
    GluA2, GluA3, and GluA1-3 (panAMPA) in pyramidal cells in the CA1 area of mouse\r\nhippocampus.
    I have found that the immunogold labeling for all of these subunits in the\r\npostsynaptic
    sites was highest in stratum radiatum and lowest in stratum lacunosummoleculare.
    The labeling density for the all subunits in the extrasynaptic sites showed a
    gradual\r\nincrease from the pyramidal cell soma towards the distal part of stratum
    radiatum. The densities\r\nof extrasynaptic GluA1, GluA2 and panAMPA labeling
    reached 10-15% of synaptic densities,\r\nwhile the ratio of extrasynaptic labeling
    for GluA3 was significantly lower compared than those\r\nfor other subunits. The
    labeling patterns for GluA1, GluA2 and GluA1-3 are similar and their\r\ndensities
    were higher in the periphery than center of synapses. In contrast, the GluA3-\r\ncontaining
    receptors were more centrally localized compared to the GluA1- and GluA2-\r\ncontaining
    receptors.\r\nThe hippocampus plays a central role in learning and memory. Contextual
    learning has been\r\nshown to require the delivery of AMPA receptors to CA1 synapses
    in the dorsal hippocampus.\r\nHowever, proximodistal heterogeneity of this plasticity
    and particular contribution of different\r\nAMPA receptor subunits are not fully
    understood. By combining inhibitory avoidance task, a\r\nhippocampus-dependent
    contextual fear-learning paradigm, with SDS-FRL, I have revealed an\r\nincrease
    in synaptic density specific to GluA1-containing AMPA receptors in the CA1 area.\r\nThe
    intrasynaptic distribution of GluA1 also changed from the periphery to center-preferred\r\npattern.
    Furthermore, this synaptic plasticity was evident selectively in stratum radiatum
    but\r\nnot stratum oriens, and in the CA1 subregion proximal but not distal to
    CA2. These findings\r\nfurther contribute to our understanding of how specific
    hippocampal subregions and AMPA\r\nreceptor subunits are involved in physiological
    learning.\r\nAlthough the immunolabeling results above shed light on subunit-specific
    plasticity in\r\nAMPAR distribution, no tools to visualize and study the subunit
    composition at the single\r\nchannel level in situ have been available. Electron
    microscopy with conventional immunogold\r\nlabeling approaches has limitations
    in the single channel analysis because of the large size of\r\nantibodies and
    steric hindrance hampering multiple subunit labeling of single channels. I\r\nmanaged
    to develop a new chemical labeling system using a short peptide tag and small\r\nsynthetic
    probes, which form specific covalent bond with a cysteine residue in the tag fused
    to\r\nproteins of interest (reactive tag system). I additionally made substantial
    progress into adapting\r\nthis system for AMPA receptor subunits.@eng"
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Marijo
      foaf_name: Jevtic, Marijo
      foaf_surname: Jevtic
      foaf_workInfoHomepage: http://www.librecat.org/personId=4BE3BC94-F248-11E8-B48F-1D18A9856A87
  bibo_doi: 10.15479/at:ista:11393
  dct_date: 2022^xs_gYear
  dct_isPartOf:
  - http://id.crossref.org/issn/2663-337X
  dct_language: eng
  dct_publisher: Institute of Science and Technology Austria@
  dct_title: Contextual fear learning induced changes in AMPA receptor subtypes along
    the proximodistal axis in dorsal hippocampus@
...