---
res:
  bibo_abstract:
  - Studies of protein fitness landscapes reveal biophysical constraints guiding protein
    evolution and empower prediction of functional proteins. However, generalisation
    of these findings is limited due to scarceness of systematic data on fitness landscapes
    of proteins with a defined evolutionary relationship. We characterized the fitness
    peaks of four orthologous fluorescent proteins with a broad range of sequence
    divergence. While two of the four studied fitness peaks were sharp, the other
    two were considerably flatter, being almost entirely free of epistatic interactions.
    Mutationally robust proteins, characterized by a flat fitness peak, were not optimal
    templates for machine-learning-driven protein design – instead, predictions were
    more accurate for fragile proteins with epistatic landscapes. Our work paves insights
    for practical application of fitness landscape heterogeneity in protein engineering.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Louisa
      foaf_name: Gonzalez Somermeyer, Louisa
      foaf_surname: Gonzalez Somermeyer
      foaf_workInfoHomepage: http://www.librecat.org/personId=4720D23C-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0001-9139-5383
  - foaf_Person:
      foaf_givenName: Aubin
      foaf_name: Fleiss, Aubin
      foaf_surname: Fleiss
  - foaf_Person:
      foaf_givenName: Alexander S
      foaf_name: Mishin, Alexander S
      foaf_surname: Mishin
  - foaf_Person:
      foaf_givenName: Nina G
      foaf_name: Bozhanova, Nina G
      foaf_surname: Bozhanova
  - foaf_Person:
      foaf_givenName: Anna A
      foaf_name: Igolkina, Anna A
      foaf_surname: Igolkina
  - foaf_Person:
      foaf_givenName: Jens
      foaf_name: Meiler, Jens
      foaf_surname: Meiler
  - foaf_Person:
      foaf_givenName: Maria-Elisenda
      foaf_name: Alaball Pujol, Maria-Elisenda
      foaf_surname: Alaball Pujol
  - foaf_Person:
      foaf_givenName: Ekaterina V
      foaf_name: Putintseva, Ekaterina V
      foaf_surname: Putintseva
  - foaf_Person:
      foaf_givenName: Karen S
      foaf_name: Sarkisyan, Karen S
      foaf_surname: Sarkisyan
  - foaf_Person:
      foaf_givenName: Fyodor
      foaf_name: Kondrashov, Fyodor
      foaf_surname: Kondrashov
      foaf_workInfoHomepage: http://www.librecat.org/personId=44FDEF62-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0001-8243-4694
  bibo_doi: 10.7554/elife.75842
  bibo_volume: 11
  dct_date: 2022^xs_gYear
  dct_identifier:
  - UT:000799197200001
  dct_isPartOf:
  - http://id.crossref.org/issn/2050-084X
  dct_language: eng
  dct_publisher: eLife Sciences Publications@
  dct_subject:
  - General Immunology and Microbiology
  - General Biochemistry
  - Genetics and Molecular Biology
  - General Medicine
  - General Neuroscience
  dct_title: Heterogeneity of the GFP fitness landscape and data-driven protein design@
  fabio_hasPubmedId: '35510622'
...