--- res: bibo_abstract: - Mutations are acquired frequently, such that each cell's genome inscribes its history of cell divisions. Common genomic alterations involve loss of heterozygosity (LOH). LOH accumulates throughout the genome, offering large encoding capacity for inferring cell lineage. Using only single-cell RNA sequencing (scRNA-seq) of mouse brain cells, we found that LOH events spanning multiple genes are revealed as tracts of monoallelically expressed, constitutionally heterozygous single-nucleotide variants (SNVs). We simultaneously inferred cell lineage and marked developmental time points based on X chromosome inactivation and the total number of LOH events while identifying cell types from gene expression patterns. Our results are consistent with progenitor cells giving rise to multiple cortical cell types through stereotyped expansion and distinct waves of neurogenesis. This type of retrospective analysis could be incorporated into scRNA-seq pipelines and, compared with experimental approaches for determining lineage in model organisms, is applicable where genetic engineering is prohibited, such as humans.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Donovan J. foaf_name: Anderson, Donovan J. foaf_surname: Anderson - foaf_Person: foaf_givenName: Florian foaf_name: Pauler, Florian foaf_surname: Pauler foaf_workInfoHomepage: http://www.librecat.org/personId=48EA0138-F248-11E8-B48F-1D18A9856A87 - foaf_Person: foaf_givenName: Aaron foaf_name: Mckenna, Aaron foaf_surname: Mckenna - foaf_Person: foaf_givenName: Jay foaf_name: Shendure, Jay foaf_surname: Shendure - foaf_Person: foaf_givenName: Simon foaf_name: Hippenmeyer, Simon foaf_surname: Hippenmeyer foaf_workInfoHomepage: http://www.librecat.org/personId=37B36620-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0003-2279-1061 - foaf_Person: foaf_givenName: Marshall S. foaf_name: Horwitz, Marshall S. foaf_surname: Horwitz bibo_doi: 10.1016/j.cels.2022.03.006 bibo_issue: '6' bibo_volume: 13 dct_date: 2022^xs_gYear dct_identifier: - UT:000814124400002 dct_isPartOf: - http://id.crossref.org/issn/2405-4712 - http://id.crossref.org/issn/2405-4720 dct_language: eng dct_publisher: Elsevier@ dct_title: Simultaneous brain cell type and lineage determined by scRNA-seq reveals stereotyped cortical development@ fabio_hasPubmedId: '35452605' ...