---
res:
  bibo_abstract:
  - The proteomes of specialized structures, and the interactomes of proteins of interest,
    provide entry points to elucidate the functions of molecular machines. Here, we
    review a proximity-labeling strategy that uses the improved E. coli biotin ligase
    TurboID to characterize C. elegans protein complexes. Although the focus is on
    C. elegans neurons, the method is applicable regardless of cell type. We describe
    detailed extraction procedures that solubilize the bulk of C. elegans proteins
    and highlight the importance of tagging endogenous genes, to ensure physiological
    expression levels. We review issues associated with non-specific background noise
    and the importance of appropriate controls. As proof of principle, we review our
    analysis of the interactome of a presynaptic active zone protein, ELKS-1. Our
    aim is to provide a detailed protocol for TurboID-based proximity labeling in
    C. elegans and to highlight its potential and its limitations to characterize
    protein complexes and subcellular compartments in this animal.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Murat
      foaf_name: Artan, Murat
      foaf_surname: Artan
      foaf_workInfoHomepage: http://www.librecat.org/personId=C407B586-6052-11E9-B3AE-7006E6697425
    orcid: 0000-0001-8945-6992
  - foaf_Person:
      foaf_givenName: Mario
      foaf_name: de Bono, Mario
      foaf_surname: de Bono
      foaf_workInfoHomepage: http://www.librecat.org/personId=4E3FF80E-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0001-8347-0443
  bibo_doi: 10.1007/978-1-0716-2321-3_15
  bibo_volume: 181
  dct_date: 2022^xs_gYear
  dct_isPartOf:
  - http://id.crossref.org/issn/0893-2336
  - http://id.crossref.org/issn/1940-6045
  - http://id.crossref.org/issn/9781071623206
  dct_language: eng
  dct_publisher: Springer Nature@
  dct_title: Proteomic Analysis of C. Elegans Neurons Using TurboID-Based Proximity
    Labeling@
...